3wxj - Revision history

OCA at 08:47, 20 March 2024

OCA — Wed, 20 Mar 2024 08:47:43 GMT

←Older revision		Revision as of 08:47, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3wxj' size='340' side='right'caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3wxj' size='340' side='right'caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Trybg Trybg~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_gambiense Trypanosoma brucei gambiense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=G3P:SN~~-~~GLYCEROL-3-PHOSPHATE'>G3P</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gk ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~31285 TRYBG])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [https://pdbe.org/3wxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [https://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [https://pdbe.org/3wxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [https://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/D3KVM3_TRYBG D3KVM3_TRYBG]
-	The glycerol kinase (GK) of African human trypanosomes is compartmentalized in their glycosomes. Unlike the host GK, which under physiological conditions catalyzes only the forward reaction (ATP-dependent glycerol phosphorylation), trypanosome GK can additionally catalyze the reverse reaction. In fact, owing to this unique reverse catalysis, GK is potentially essential for the parasites survival in the human host, hence a promising drug target. The mechanism of its reverse catalysis was unknown; therefore, it was not clear if this ability was purely due to its localization in the organelles or whether structure-based catalytic differences also contribute. To investigate this lack of information, the X-ray crystal structure of this protein was determined up to 1.90 A resolution, in its unligated form and in complex with three natural ligands. These data, in conjunction with results from structure-guided mutagenesis suggests that the trypanosome GK is possibly a transiently autophosphorylating threonine kinase, with the catalytic site formed by non-conserved residues. Our results provide a series of structural peculiarities of this enzyme, and gives unexpected insight into the reverse catalysis mechanism. Together, they provide an encouraging molecular framework for the development of trypanosome GK-specific inhibitors, which may lead to the design of new and safer trypanocidal drug(s).	+
-		+
-	Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase.,Balogun EO, Inaoka DK, Shiba T, Kido Y, Tsuge C, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Michels PA, Kita K, Harada S Mol Microbiol. 2014 Dec;94(6):~~1315-29. doi: 10.1111~~/~~mmi.12831. Epub 2014 Nov 4. PMID:25315291<ref>PMID:25315291<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3wxj" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Glycerol kinase\|Glycerol kinase]]		*[[Glycerol kinase\|Glycerol kinase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glycerol kinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Trybg~~]]	+	[[Category: Trypanosoma brucei gambiense]]
-	[[Category: Aoki, T]]	+	[[Category: Aoki T]]
-	[[Category: Balogun~~, E O~~]]	+	[[Category: Balogun EO]]
-	[[Category: Harada, S]]	+	[[Category: Harada S]]
-	[[Category: Honma, T]]	+	[[Category: Honma T]]
-	[[Category: Inaoka~~, D K~~]]	+	[[Category: Inaoka DK]]
-	[[Category: Inoue, M]]	+	[[Category: Inoue M]]
-	[[Category: Kido, Y]]	+	[[Category: Kido Y]]
-	[[Category: Kita, K]]	+	[[Category: Kita K]]
-	[[Category: Matsuoka, S]]	+	[[Category: Matsuoka S]]
-	[[Category: Michels~~, P A.M~~]]	+	[[Category: Michels PAM]]
-	[[Category: Nara, T]]	+	[[Category: Nara T]]
-	[[Category: Shiba, T]]	+	[[Category: Shiba T]]
-	[[Category: Tanaka, A]]	+	[[Category: Tanaka A]]
-	[[Category: Tsuge, C]]	+	[[Category: Tsuge C]]
-	~~[[Category: Glycosome]]~~	+
-	~~[[Category: Sugar kinase superfamily]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Trypanosoma~~]]	+

OCA at 05:34, 10 August 2022

OCA — Wed, 10 Aug 2022 05:34:43 GMT

←Older revision		Revision as of 05:34, 10 August 2022
Line 1:		Line 1:

	==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==		==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==
-	<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3wxj' size='340' side='right'caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trybg Trybg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trybg Trybg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gk ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31285 TRYBG])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gk ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31285 TRYBG])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [~~http~~://pdbe.org/3wxj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [https://pdbe.org/3wxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [https://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Glycerol kinase]]		[[Category: Glycerol kinase]]
		+	[[Category: Large Structures]]
	[[Category: Trybg]]		[[Category: Trybg]]
	[[Category: Aoki, T]]		[[Category: Aoki, T]]

OCA at 09:38, 21 February 2019

OCA — Thu, 21 Feb 2019 09:38:24 GMT

←Older revision		Revision as of 09:38, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybg Trybg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31285 TRYBG])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://pdbe.org/3wxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://pdbe.org/3wxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
Line 26:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Glycerol kinase]]		[[Category: Glycerol kinase]]
		+	[[Category: Trybg]]
	[[Category: Aoki, T]]		[[Category: Aoki, T]]
	[[Category: Balogun, E O]]		[[Category: Balogun, E O]]

OCA at 15:20, 5 August 2016

OCA — Fri, 05 Aug 2016 15:20:55 GMT

←Older revision		Revision as of 15:20, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==		==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==
	<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://pdbe.org/3wxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wxj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycerol kinase\|Glycerol kinase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:46, 31 December 2014

OCA — Wed, 31 Dec 2014 14:46:25 GMT

←Older revision		Revision as of 14:46, 31 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==
		+	<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wxi\|3wxi]], [[3wxk\|3wxk]], [[3wxl\|3wxl]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The glycerol kinase (GK) of African human trypanosomes is compartmentalized in their glycosomes. Unlike the host GK, which under physiological conditions catalyzes only the forward reaction (ATP-dependent glycerol phosphorylation), trypanosome GK can additionally catalyze the reverse reaction. In fact, owing to this unique reverse catalysis, GK is potentially essential for the parasites survival in the human host, hence a promising drug target. The mechanism of its reverse catalysis was unknown; therefore, it was not clear if this ability was purely due to its localization in the organelles or whether structure-based catalytic differences also contribute. To investigate this lack of information, the X-ray crystal structure of this protein was determined up to 1.90 A resolution, in its unligated form and in complex with three natural ligands. These data, in conjunction with results from structure-guided mutagenesis suggests that the trypanosome GK is possibly a transiently autophosphorylating threonine kinase, with the catalytic site formed by non-conserved residues. Our results provide a series of structural peculiarities of this enzyme, and gives unexpected insight into the reverse catalysis mechanism. Together, they provide an encouraging molecular framework for the development of trypanosome GK-specific inhibitors, which may lead to the design of new and safer trypanocidal drug(s).

-	~~The entry 3wxj is ON HOLD~~	+	Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase.,Balogun EO, Inaoka DK, Shiba T, Kido Y, Tsuge C, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Michels PA, Kita K, Harada S Mol Microbiol. 2014 Dec;94(6):1315-29. doi: 10.1111/mmi.12831. Epub 2014 Nov 4. PMID:25315291<ref>PMID:25315291</ref>

-	~~Authors: Balogun~~, E.~~O., Inaoka, D.K., Shiba, T., Kido, Y., Tsuge, C., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka,~~ S.~~, Michels, P.A.M., Kita, K., Harada, S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of trypanosoma brucei gambiense glycerol~~ kinase ~~in complex with glycerol 3-phosphate~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glycerol kinase]]
		+	[[Category: Aoki, T]]
		+	[[Category: Balogun, E O]]
		+	[[Category: Harada, S]]
	[[Category: Honma, T]]		[[Category: Honma, T]]
		+	[[Category: Inaoka, D K]]
		+	[[Category: Inoue, M]]
		+	[[Category: Kido, Y]]
		+	[[Category: Kita, K]]
	[[Category: Matsuoka, S]]		[[Category: Matsuoka, S]]
		+	[[Category: Michels, P A.M]]
		+	[[Category: Nara, T]]
	[[Category: Shiba, T]]		[[Category: Shiba, T]]
-	[[Category: ~~Inaoka~~, ~~D.K]]~~	+	[[Category: Tanaka, A]]
-	~~[[Category: Kita, K]]~~	+
-	~~[[Category: Michels, P.~~A.M]]	+
	[[Category: Tsuge, C]]		[[Category: Tsuge, C]]
-	[[Category: ~~Nara, T~~]]	+	[[Category: Glycosome]]
-	[[Category: ~~Balogun, E.O~~]]	+	[[Category: Sugar kinase superfamily]]
-	[[Category: ~~Tanaka, A~~]]	+	[[Category: Transferase]]
-	[[Category: ~~Harada, S]]~~	+	[[Category: Trypanosoma]]
-	~~[[Category: Inoue, M]]~~	+
-	~~[[Category: Aoki, T]]~~	+
-	~~[[Category: Kido, Y~~]]	+

OCA at 13:04, 24 December 2014

OCA — Wed, 24 Dec 2014 13:04:39 GMT

←Older revision		Revision as of 13:04, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate		Description: Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate
		+	[[Category: Unreleased Structures]]
		+	[[Category: Honma, T]]
		+	[[Category: Matsuoka, S]]
		+	[[Category: Shiba, T]]
		+	[[Category: Inaoka, D.K]]
		+	[[Category: Kita, K]]
		+	[[Category: Michels, P.A.M]]
		+	[[Category: Tsuge, C]]
		+	[[Category: Nara, T]]
		+	[[Category: Balogun, E.O]]
		+	[[Category: Tanaka, A]]
		+	[[Category: Harada, S]]
		+	[[Category: Inoue, M]]
		+	[[Category: Aoki, T]]
		+	[[Category: Kido, Y]]

OCA at 06:12, 27 August 2014

OCA — Wed, 27 Aug 2014 06:12:24 GMT

←Older revision		Revision as of 06:12, 27 August 2014
Line 3:		Line 3:
	The entry 3wxj is ON HOLD		The entry 3wxj is ON HOLD

-	Authors: Balogun, E.O. , Inaoka, D.K. , Shiba, T. , Kido, Y. , Tsuge, C. , Nara, T. , Aoki, T. , Honma, T. , Tanaka, A. , Inoue, M. , Matsuoka, S. , Michels, P.A.M. , Kita, K. , Harada, S.	+	Authors: Balogun, E.O., Inaoka, D.K., Shiba, T., Kido, Y., Tsuge, C., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka, S., Michels, P.A.M., Kita, K., Harada, S.

-	Description: ~~CRYSTAL STRUCTURE OF TRYPANOSOMA BRUCEI GAMBIENSE GLYCEROL KINASE IN COMPLEX WITH GLYCEROL~~ 3-~~PHOSPHATE~~	+	Description: Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate

OCA: Protected "3wxj" [edit=sysop:move=sysop]

OCA — Wed, 13 Aug 2014 07:02:08 GMT

Protected "3wxj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:02, 13 August 2014

OCA: New page: '''Unreleased structure''' The entry 3wxj is ON HOLD Authors: Balogun, E.O. , Inaoka, D.K. , Shiba, T. , Kido, Y. , Tsuge, C. , Nara, T. , Aoki, T. , Honma, T. , Tanaka, A. , Inoue, M. ...

OCA — Wed, 13 Aug 2014 07:02:06 GMT

New page: '''Unreleased structure''' The entry 3wxj is ON HOLD Authors: Balogun, E.O. , Inaoka, D.K. , Shiba, T. , Kido, Y. , Tsuge, C. , Nara, T. , Aoki, T. , Honma, T. , Tanaka, A. , Inoue, M. ...

New page

'''Unreleased structure'''

The entry 3wxj is ON HOLD

Authors: Balogun, E.O. , Inaoka, D.K. , Shiba, T. , Kido, Y. , Tsuge, C. , Nara, T. , Aoki, T. , Honma, T. , Tanaka, A. , Inoue, M. , Matsuoka, S. , Michels, P.A.M. , Kita, K. , Harada, S.

Description: CRYSTAL STRUCTURE OF TRYPANOSOMA BRUCEI GAMBIENSE GLYCEROL KINASE IN COMPLEX WITH GLYCEROL 3-PHOSPHATE