3wyq - Revision history

OCA at 19:22, 29 May 2024

OCA — Wed, 29 May 2024 19:22:47 GMT

←Older revision		Revision as of 19:22, 29 May 2024
Line 1:		Line 1:

	==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==		==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==
-	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]]' scene=''>	+	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [https://pdbe.org/3wyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [https://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [https://pdbe.org/3wyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [https://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We previously created a low-immunogenic core streptavidin mutant No. 314 (LISA-314) by replacing six amino-acid residues for use as a delivery tool for an antibody multistep pre-targeting process (Yumura et al., Protein science, 22, 213-221, 2013). Here, we performed high-resolution X-ray structural analyses of LISA-314 and wild-type streptavidin to investigate the effect of substitutions on the protein function and the three-dimensional structure. LISA-314 forms a tetramer in the same manner as wild-type streptavidin. The binding mode of d-biotin in LISA-314 is also completely identical to that in wild-type streptavidin, and conformational changes were observed mostly at the side chains of substituted sites. Any large conformational changes corresponding to the reduction of B factors around the substituted sites were not observed. These results demonstrated the LISA-314 acquired low immunogenicity without losing structural properties of original wild-type streptavidin.
		+
		+	Crystal structure of streptavidin mutant with low immunogenicity.,Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T, Sugiyama A J Biosci Bioeng. 2014 Nov 26. pii: S1389-1723(14)00403-4. doi:, 10.1016/j.jbiosc.2014.10.025. PMID:25434833<ref>PMID:25434833</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wyq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces avidinii]]
	[[Category: Doi H]]		[[Category: Doi H]]
	[[Category: Inoue T]]		[[Category: Inoue T]]

OCA at 10:38, 31 August 2022

OCA — Wed, 31 Aug 2022 10:38:39 GMT

←Older revision		Revision as of 10:38, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==		==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==
-	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]]~~, [[Resolution\|resolution]] 1.00Å~~' scene=''>	+	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3wyq]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [https://pdbe.org/3wyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [https://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wyp\|3wyp]]</div></td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [https://pdbe.org/3wyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [https://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We previously created a low-immunogenic core streptavidin mutant No. 314 (LISA-314) by replacing six amino-acid residues for use as a delivery tool for an antibody multistep pre-targeting process (Yumura et al., Protein science, 22, 213-221, 2013). Here, we performed high-resolution X-ray structural analyses of LISA-314 and wild-type streptavidin to investigate the effect of substitutions on the protein function and the three-dimensional structure. LISA-314 forms a tetramer in the same manner as wild-type streptavidin. The binding mode of d-biotin in LISA-314 is also completely identical to that in wild-type streptavidin, and conformational changes were observed mostly at the side chains of substituted sites. Any large conformational changes corresponding to the reduction of B factors around the substituted sites were not observed. These results demonstrated the LISA-314 acquired low immunogenicity without losing structural properties of original wild-type streptavidin.
-
-	Crystal structure of streptavidin mutant with low immunogenicity.,Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T, Sugiyama A J Biosci Bioeng. 2014 Nov 26. pii: S1389-1723(14)00403-4. doi:, 10.1016/j.jbiosc.2014.10.025. PMID:25434833<ref>PMID:25434833</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3wyq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Doi, H]]	+	[[Category: Doi H]]
-	[[Category: Inoue, T]]	+	[[Category: Inoue T]]
-	[[Category: Kawamura, T]]	+	[[Category: Kawamura T]]
-	[[Category: Kawato, T]]	+	[[Category: Kawato T]]
-	[[Category: Kodama, T]]	+	[[Category: Kodama T]]
-	[[Category: Matsumura, H]]	+	[[Category: Matsumura H]]
-	[[Category: Meshizuka, T]]	+	[[Category: Meshizuka T]]
-	[[Category: Mizohata, E]]	+	[[Category: Mizohata E]]
-	[[Category: Sugiyama, A]]	+	[[Category: Sugiyama A]]
-	[[Category: Tsumoto, K]]	+	[[Category: Tsumoto K]]
-	[[Category: Yumura, K]]	+	[[Category: Yumura K]]
-	~~[[Category: Beta-barrel]]~~	+
-	~~[[Category: Biotin binding protein~~]]	+

OCA at 05:00, 25 August 2022

OCA — Thu, 25 Aug 2022 05:00:17 GMT

←Older revision		Revision as of 05:00, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wyp\|3wyp]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wyp\|3wyp]]</div></td></tr>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Doi, H]]		[[Category: Doi, H]]

OCA at 05:35, 10 August 2022

OCA — Wed, 10 Aug 2022 05:35:54 GMT

←Older revision		Revision as of 05:35, 10 August 2022
Line 1:		Line 1:

	==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==		==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==
-	<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>	+	<StructureSection load='3wyq' size='340' side='right'caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wyp\|3wyp]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [~~http~~://pdbe.org/3wyq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [https://pdbe.org/3wyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [https://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3wyq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3wyq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Doi, H]]		[[Category: Doi, H]]
	[[Category: Inoue, T]]		[[Category: Inoue, T]]

OCA at 09:45, 21 February 2019

OCA — Thu, 21 Feb 2019 09:45:16 GMT

←Older revision		Revision as of 09:45, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
	[[Category: Doi, H]]		[[Category: Doi, H]]
	[[Category: Inoue, T]]		[[Category: Inoue, T]]

OCA at 07:48, 5 August 2016

OCA — Fri, 05 Aug 2016 07:48:04 GMT

←Older revision		Revision as of 07:48, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==		==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==
	<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [http://www.ebi.ac.uk/pdbsum/3wyq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [http://pdbe.org/3wyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [http://www.ebi.ac.uk/pdbsum/3wyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wyq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:59, 24 December 2014

OCA — Wed, 24 Dec 2014 10:59:00 GMT

←Older revision		Revision as of 10:59, 24 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution==
		+	<StructureSection load='3wyq' size='340' side='right' caption='[[3wyq]], [[Resolution\|resolution]] 1.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wyq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WYQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wyp\|3wyp]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wyq RCSB], [http://www.ebi.ac.uk/pdbsum/3wyq PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We previously created a low-immunogenic core streptavidin mutant No. 314 (LISA-314) by replacing six amino-acid residues for use as a delivery tool for an antibody multistep pre-targeting process (Yumura et al., Protein science, 22, 213-221, 2013). Here, we performed high-resolution X-ray structural analyses of LISA-314 and wild-type streptavidin to investigate the effect of substitutions on the protein function and the three-dimensional structure. LISA-314 forms a tetramer in the same manner as wild-type streptavidin. The binding mode of d-biotin in LISA-314 is also completely identical to that in wild-type streptavidin, and conformational changes were observed mostly at the side chains of substituted sites. Any large conformational changes corresponding to the reduction of B factors around the substituted sites were not observed. These results demonstrated the LISA-314 acquired low immunogenicity without losing structural properties of original wild-type streptavidin.

-	~~The entry 3wyq is ON HOLD~~	+	Crystal structure of streptavidin mutant with low immunogenicity.,Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T, Sugiyama A J Biosci Bioeng. 2014 Nov 26. pii: S1389-1723(14)00403-4. doi:, 10.1016/j.jbiosc.2014.10.025. PMID:25434833<ref>PMID:25434833</ref>

-	~~Authors: Kawato~~, T.~~, Mizohata, E~~.~~, Meshizuka, T~~., Doi, H., Kawamura, T~~., Matsumura, H., Yumura, K., Tsumoto, K.~~, Kodama, T., ~~Inoue~~, T., Sugiyama, A.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the low~~-~~immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Doi, H]]
		+	[[Category: Inoue, T]]
		+	[[Category: Kawamura, T]]
		+	[[Category: Kawato, T]]
		+	[[Category: Kodama, T]]
		+	[[Category: Matsumura, H]]
		+	[[Category: Meshizuka, T]]
		+	[[Category: Mizohata, E]]
		+	[[Category: Sugiyama, A]]
		+	[[Category: Tsumoto, K]]
		+	[[Category: Yumura, K]]
		+	[[Category: Beta-barrel]]
		+	[[Category: Biotin binding protein]]

OCA: Protected "3wyq" [edit=sysop:move=sysop]

OCA — Wed, 24 Sep 2014 07:37:17 GMT

Protected "3wyq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:37, 24 September 2014

OCA: New page: '''Unreleased structure''' The entry 3wyq is ON HOLD Authors: Kawato, T., Mizohata, E., Meshizuka, T., Doi, H., Kawamura, T., Matsumura, H., Yumura, K., Tsumoto, K., Kodama, T., Inoue, ...

OCA — Wed, 24 Sep 2014 07:37:16 GMT

New page: '''Unreleased structure''' The entry 3wyq is ON HOLD Authors: Kawato, T., Mizohata, E., Meshizuka, T., Doi, H., Kawamura, T., Matsumura, H., Yumura, K., Tsumoto, K., Kodama, T., Inoue, ...

New page

'''Unreleased structure'''

The entry 3wyq is ON HOLD

Authors: Kawato, T., Mizohata, E., Meshizuka, T., Doi, H., Kawamura, T., Matsumura, H., Yumura, K., Tsumoto, K., Kodama, T., Inoue, T., Sugiyama, A.

Description: Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution