3x0h - Revision history

OCA: Redirecting to 5b5s

OCA — Fri, 09 Dec 2016 19:19:02 GMT

Redirecting to 5b5s

←Older revision		Revision as of 19:19, 9 December 2016
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-	~~==Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus==~~	+	#REDIRECT [[5b5s]] This PDB entry is obsolete and replaced by 5b5s
-	~~<StructureSection load='3x0h' size='340' side='right' caption='~~[[~~3x0h]], [[Resolution\|resolution]] 1.50Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[3x0h~~]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0H FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0h OCA], [http://pdbe.org/3x0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0h RCSB], [http://www.ebi.ac.uk/pdbsum/3x0h PDBsum]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Carbohydrate esterase catalyzes the de-O or de-N-acylation of substituted saccharides in plant cell walls~~ and thus has great potential for industrial biomass saccharification. We recently identified the putative carbohydrate esterase family 3 (CE3) from Talaromyces cellulolyticus. Here, we prepared the recombinant catalytic domain of the enzyme and crystallized it. The crystal structure was determined to 1.5 A resolution. From the structural analysis, it was elucidated that a n-octyl-beta-D-glucopyranoside bound to near the catalytic triad (Ser10, Asp179 and His182) and was buried in the active site cavity. Site-directed mutagenesis showed that the N-terminal disulfide bond located near the catalytic triad is involved in the activity and structural stability of the enzyme.	+
-		+
-	Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site.,Watanabe M, Fukada H, Inoue H, Ishikawa K FEBS Lett. 2015 May 8;589(11):1200-6. doi: 10.1016/j.febslet.2015.03.020. Epub, 2015 Mar 28. PMID:25825334<ref>PMID:25825334</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3x0h" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Ishikawa, K]]~~	+
-	~~[[Category: Watanabe, M]]~~	+
-	~~[[Category: Hydrolase]]~~	+

OCA at 05:26, 22 October 2015

OCA — Thu, 22 Oct 2015 05:26:09 GMT

←Older revision		Revision as of 05:26, 22 October 2015
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus==
		+	<StructureSection load='3x0h' size='340' side='right' caption='[[3x0h]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3x0h]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0h OCA], [http://pdbe.org/3x0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0h RCSB], [http://www.ebi.ac.uk/pdbsum/3x0h PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carbohydrate esterase catalyzes the de-O or de-N-acylation of substituted saccharides in plant cell walls and thus has great potential for industrial biomass saccharification. We recently identified the putative carbohydrate esterase family 3 (CE3) from Talaromyces cellulolyticus. Here, we prepared the recombinant catalytic domain of the enzyme and crystallized it. The crystal structure was determined to 1.5 A resolution. From the structural analysis, it was elucidated that a n-octyl-beta-D-glucopyranoside bound to near the catalytic triad (Ser10, Asp179 and His182) and was buried in the active site cavity. Site-directed mutagenesis showed that the N-terminal disulfide bond located near the catalytic triad is involved in the activity and structural stability of the enzyme.

-	~~The entry 3x0h is ON HOLD~~	+	Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site.,Watanabe M, Fukada H, Inoue H, Ishikawa K FEBS Lett. 2015 May 8;589(11):1200-6. doi: 10.1016/j.febslet.2015.03.020. Epub, 2015 Mar 28. PMID:25825334<ref>PMID:25825334</ref>

-	~~Authors: Watanabe~~, M.~~, Ishikawa, K~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus~~	+	<div class="pdbe-citations 3x0h" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ishikawa, K]]
	[[Category: Watanabe, M]]		[[Category: Watanabe, M]]
-	[[Category: ~~Ishikawa, K~~]]	+	[[Category: Hydrolase]]

OCA at 13:11, 24 December 2014

OCA — Wed, 24 Dec 2014 13:11:17 GMT

←Older revision		Revision as of 13:11, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus		Description: Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus
		+	[[Category: Unreleased Structures]]
		+	[[Category: Watanabe, M]]
		+	[[Category: Ishikawa, K]]

OCA: Protected "3x0h" [edit=sysop:move=sysop]

OCA — Wed, 22 Oct 2014 09:07:25 GMT

Protected "3x0h" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:07, 22 October 2014

OCA: New page: '''Unreleased structure''' The entry 3x0h is ON HOLD Authors: Watanabe, M., Ishikawa, K. Description: Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyti...

OCA — Wed, 22 Oct 2014 09:07:23 GMT

New page: '''Unreleased structure''' The entry 3x0h is ON HOLD Authors: Watanabe, M., Ishikawa, K. Description: Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyti...

New page

'''Unreleased structure'''

The entry 3x0h is ON HOLD

Authors: Watanabe, M., Ishikawa, K.

Description: Crystal structure of a carbohydrate esterase family 1 from Talaromyces cellulolyticus