3x0w - Revision history

OCA at 13:38, 8 November 2023

OCA — Wed, 08 Nov 2023 13:38:32 GMT

←Older revision		Revision as of 13:38, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==		==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
-	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]]' scene=''>	+	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.71Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lysosome is the final destination for degradation of endocytic cargo, plasma membrane constituents, and intracellular components sequestered by macroautophagy. Fusion of endosomes and autophagosomes with the lysosome depends on the GTPase Rab7 and the homotypic fusion and protein sorting (HOPS) complex, but adaptor proteins that link endocytic and autophagy pathways with lysosomes are poorly characterized. Herein, we show that Pleckstrin homology domain containing protein family member 1 (PLEKHM1) directly interacts with HOPS complex and contains a LC3-interacting region (LIR) that mediates its binding to autophagosomal membranes. Depletion of PLEKHM1 blocks lysosomal degradation of endocytic (EGFR) cargo and enhances presentation of MHC class I molecules. Moreover, genetic loss of PLEKHM1 impedes autophagy flux upon mTOR inhibition and PLEKHM1 regulates clearance of protein aggregates in an autophagy- and LIR-dependent manner. PLEKHM1 is thus a multivalent endocytic adaptor involved in the lysosome fusion events controlling selective and nonselective autophagy pathways.
		+
		+	PLEKHM1 Regulates Autophagosome-Lysosome Fusion through HOPS Complex and LC3/GABARAP Proteins.,McEwan DG, Popovic D, Gubas A, Terawaki S, Suzuki H, Stadel D, Coxon FP, Miranda de Stegmann D, Bhogaraju S, Maddi K, Kirchof A, Gatti E, Helfrich MH, Wakatsuki S, Behrends C, Pierre P, Dikic I Mol Cell. 2015 Jan 8;57(1):39-54. doi: 10.1016/j.molcel.2014.11.006. Epub 2014, Dec 11. PMID:25498145<ref>PMID:25498145</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]		*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Behrends C]]		[[Category: Behrends C]]

OCA at 10:40, 31 August 2022

OCA — Wed, 31 Aug 2022 10:40:26 GMT

←Older revision		Revision as of 10:40, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==		==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
-	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]]~~, [[Resolution\|resolution]] 2.71Å~~' scene=''>	+	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3x0w]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The lysosome is the final destination for degradation of endocytic cargo, plasma membrane constituents, and intracellular components sequestered by macroautophagy. Fusion of endosomes and autophagosomes with the lysosome depends on the GTPase Rab7 and the homotypic fusion and protein sorting (HOPS) complex, but adaptor proteins that link endocytic and autophagy pathways with lysosomes are poorly characterized. Herein, we show that Pleckstrin homology domain containing protein family member 1 (PLEKHM1) directly interacts with HOPS complex and contains a LC3-interacting region (LIR) that mediates its binding to autophagosomal membranes. Depletion of PLEKHM1 blocks lysosomal degradation of endocytic (EGFR) cargo and enhances presentation of MHC class I molecules. Moreover, genetic loss of PLEKHM1 impedes autophagy flux upon mTOR inhibition and PLEKHM1 regulates clearance of protein aggregates in an autophagy- and LIR-dependent manner. PLEKHM1 is thus a multivalent endocytic adaptor involved in the lysosome fusion events controlling selective and nonselective autophagy pathways.
-
-	PLEKHM1 Regulates Autophagosome-Lysosome Fusion through HOPS Complex and LC3/GABARAP Proteins.,McEwan DG, Popovic D, Gubas A, Terawaki S, Suzuki H, Stadel D, Coxon FP, Miranda de Stegmann D, Bhogaraju S, Maddi K, Kirchof A, Gatti E, Helfrich MH, Wakatsuki S, Behrends C, Pierre P, Dikic I Mol Cell. 2015 Jan 8;57(1):39-54. doi: 10.1016/j.molcel.2014.11.006. Epub 2014, Dec 11. PMID:25498145<ref>PMID:25498145</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]		*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Behrends, C]]	+	[[Category: Behrends C]]
-	[[Category: Bhogaraju, S]]	+	[[Category: Bhogaraju S]]
-	[[Category: Coxon, F]]	+	[[Category: Coxon F]]
-	[[Category: Dikic, I]]	+	[[Category: Dikic I]]
-	[[Category: Gatti, E]]	+	[[Category: Gatti E]]
-	[[Category: Gubas, A]]	+	[[Category: Gubas A]]
-	[[Category: Helfrich~~, M H~~]]	+	[[Category: Helfrich MH]]
-	[[Category: Kirchhoff, A]]	+	[[Category: Kirchhoff A]]
-	[[Category: Maddi, K]]	+	[[Category: Maddi K]]
-	[[Category: McEwan~~, D G~~]]	+	[[Category: McEwan DG]]
-	[[Category: Pierre, P]]	+	[[Category: Pierre P]]
-	[[Category: Popovic, D]]	+	[[Category: Popovic D]]
-	[[Category: Stadel, D]]	+	[[Category: Stadel D]]
-	[[Category: Stegmann~~, D M~~]]	+	[[Category: Stegmann DM]]
-	[[Category: Suzuki, H]]	+	[[Category: Suzuki H]]
-	[[Category: Terawaki, S]]	+	[[Category: Terawaki S]]
-	[[Category: Wakatsuki, S]]	+	[[Category: Wakatsuki S]]
-	~~[[Category: Autophagy]]~~	+
-	~~[[Category: Plekhm1]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Ubiquitin-like fold~~]]	+

OCA at 05:01, 25 August 2022

OCA — Thu, 25 Aug 2022 05:01:03 GMT

←Older revision		Revision as of 05:01, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Behrends, C]]		[[Category: Behrends, C]]

OCA at 05:38, 10 August 2022

OCA — Wed, 10 Aug 2022 05:38:09 GMT

←Older revision		Revision as of 05:38, 10 August 2022
Line 1:		Line 1:

	==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==		==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
-	<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>	+	<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3X0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [~~http~~://pdbe.org/3x0w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).	+	[[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Microtubule-associated protein\|Microtubule-associated protein]]	+	*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Behrends, C]]		[[Category: Behrends, C]]
	[[Category: Bhogaraju, S]]		[[Category: Bhogaraju, S]]

OCA at 17:12, 27 February 2019

OCA — Wed, 27 Feb 2019 17:12:00 GMT

←Older revision		Revision as of 17:12, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [http://pdbe.org/3x0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [http://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [http://pdbe.org/3x0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [http://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Microtubule-associated protein\|Microtubule-associated protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Behrends, C]]		[[Category: Behrends, C]]
	[[Category: Bhogaraju, S]]		[[Category: Bhogaraju, S]]

OCA at 21:25, 11 August 2016

OCA — Thu, 11 Aug 2016 21:25:21 GMT

←Older revision		Revision as of 21:25, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==		==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
	<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0W FirstGlance]. <br>		<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [http://www.ebi.ac.uk/pdbsum/3x0w PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [http://pdbe.org/3x0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [http://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:36, 14 January 2015

OCA — Wed, 14 Jan 2015 13:36:02 GMT

←Older revision		Revision as of 13:36, 14 January 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
		+	<StructureSection load='3x0w' size='340' side='right' caption='[[3x0w]], [[Resolution\|resolution]] 2.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [http://www.ebi.ac.uk/pdbsum/3x0w PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lysosome is the final destination for degradation of endocytic cargo, plasma membrane constituents, and intracellular components sequestered by macroautophagy. Fusion of endosomes and autophagosomes with the lysosome depends on the GTPase Rab7 and the homotypic fusion and protein sorting (HOPS) complex, but adaptor proteins that link endocytic and autophagy pathways with lysosomes are poorly characterized. Herein, we show that Pleckstrin homology domain containing protein family member 1 (PLEKHM1) directly interacts with HOPS complex and contains a LC3-interacting region (LIR) that mediates its binding to autophagosomal membranes. Depletion of PLEKHM1 blocks lysosomal degradation of endocytic (EGFR) cargo and enhances presentation of MHC class I molecules. Moreover, genetic loss of PLEKHM1 impedes autophagy flux upon mTOR inhibition and PLEKHM1 regulates clearance of protein aggregates in an autophagy- and LIR-dependent manner. PLEKHM1 is thus a multivalent endocytic adaptor involved in the lysosome fusion events controlling selective and nonselective autophagy pathways.

-	~~The entry 3x0w is ON HOLD~~	+	PLEKHM1 Regulates Autophagosome-Lysosome Fusion through HOPS Complex and LC3/GABARAP Proteins.,McEwan DG, Popovic D, Gubas A, Terawaki S, Suzuki H, Stadel D, Coxon FP, Miranda de Stegmann D, Bhogaraju S, Maddi K, Kirchof A, Gatti E, Helfrich MH, Wakatsuki S, Behrends C, Pierre P, Dikic I Mol Cell. 2015 Jan 8;57(1):39-54. doi: 10.1016/j.molcel.2014.11.006. Epub 2014, Dec 11. PMID:25498145<ref>PMID:25498145</ref>

-	~~Authors: Suzuki~~, H.~~, McEwan, D.G., Popovic, D., Gubas, A., Terawaki,~~ S.~~, Stadel, D., Coxon, F., Stegmann, D.M., Bhogaraju, S., Maddi, K., Kirchhoff, A., Gatti, E., Helfrich, M.H., Behrends, C., Pierre, P., Dikic, I., Wakatsuki, S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Maddi~~, K]]	+	__TOC__
-	[[Category: ~~Stegmann~~, ~~D.M~~]]	+	</StructureSection>
		+	[[Category: Behrends, C]]
		+	[[Category: Bhogaraju, S]]
		+	[[Category: Coxon, F]]
	[[Category: Dikic, I]]		[[Category: Dikic, I]]
-	[[Category: ~~Terawaki~~, ~~S]]~~	+	[[Category: Gatti, E]]
-	~~[[Category: Mcewan, D.G]]~~	+
-	~~[[Category: Coxon, F~~]]	+
	[[Category: Gubas, A]]		[[Category: Gubas, A]]
-	[[Category: Helfrich, M.H]]	+	[[Category: Helfrich, M H]]
-	[[Category: ~~Bhogaraju~~, S]]	+	[[Category: Kirchhoff, A]]
		+	[[Category: Maddi, K]]
		+	[[Category: McEwan, D G]]
	[[Category: Pierre, P]]		[[Category: Pierre, P]]
	[[Category: Popovic, D]]		[[Category: Popovic, D]]
-	[[Category: Wakatsuki, S]]
-	[[Category: Gatti, E]]
-	[[Category: Kirchhoff, A]]
-	[[Category: Behrends, C]]
	[[Category: Stadel, D]]		[[Category: Stadel, D]]
		+	[[Category: Stegmann, D M]]
	[[Category: Suzuki, H]]		[[Category: Suzuki, H]]
		+	[[Category: Terawaki, S]]
		+	[[Category: Wakatsuki, S]]
		+	[[Category: Autophagy]]
		+	[[Category: Plekhm1]]
		+	[[Category: Protein binding]]
		+	[[Category: Ubiquitin-like fold]]

OCA at 13:12, 24 December 2014

OCA — Wed, 24 Dec 2014 13:12:28 GMT

←Older revision		Revision as of 13:12, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119		Description: Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119
		+	[[Category: Unreleased Structures]]
		+	[[Category: Maddi, K]]
		+	[[Category: Stegmann, D.M]]
		+	[[Category: Dikic, I]]
		+	[[Category: Terawaki, S]]
		+	[[Category: Mcewan, D.G]]
		+	[[Category: Coxon, F]]
		+	[[Category: Gubas, A]]
		+	[[Category: Helfrich, M.H]]
		+	[[Category: Bhogaraju, S]]
		+	[[Category: Pierre, P]]
		+	[[Category: Popovic, D]]
		+	[[Category: Wakatsuki, S]]
		+	[[Category: Gatti, E]]
		+	[[Category: Kirchhoff, A]]
		+	[[Category: Behrends, C]]
		+	[[Category: Stadel, D]]
		+	[[Category: Suzuki, H]]

OCA: Protected "3x0w" [edit=sysop:move=sysop]

OCA — Wed, 29 Oct 2014 09:43:25 GMT

Protected "3x0w" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:43, 29 October 2014

OCA: New page: '''Unreleased structure''' The entry 3x0w is ON HOLD Authors: Suzuki, H., McEwan, D.G., Popovic, D., Gubas, A., Terawaki, S., Stadel, D., Coxon, F., Stegmann, D.M., Bhogaraju, S., Maddi...

OCA — Wed, 29 Oct 2014 09:43:23 GMT

New page: '''Unreleased structure''' The entry 3x0w is ON HOLD Authors: Suzuki, H., McEwan, D.G., Popovic, D., Gubas, A., Terawaki, S., Stadel, D., Coxon, F., Stegmann, D.M., Bhogaraju, S., Maddi...

New page

'''Unreleased structure'''

The entry 3x0w is ON HOLD

Authors: Suzuki, H., McEwan, D.G., Popovic, D., Gubas, A., Terawaki, S., Stadel, D., Coxon, F., Stegmann, D.M., Bhogaraju, S., Maddi, K., Kirchhoff, A., Gatti, E., Helfrich, M.H., Behrends, C., Pierre, P., Dikic, I., Wakatsuki, S.

Description: Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119