3zfp - Revision history

OCA at 11:00, 20 December 2023

OCA — Wed, 20 Dec 2023 11:00:51 GMT

←Older revision		Revision as of 11:00, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pestivirus_strain_d32~~/~~00_hobi~~ Pestivirus strain ~~d32~~/~~00_hobi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pestivirus_strain_D32/00_HoBi Pestivirus strain D32/00_HoBi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3zfn\|3zfn]]~~, ~~[[3zfo\|3zfo]], [[3zfq\|3zfq]], [[3zfr\|3zfr]], [[3zft\|3zft]], [[3zfu\|3zfu]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [https://pdbe.org/3zfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [https://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [https://pdbe.org/3zfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [https://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5L4B1_9FLAV Q5L4B1_9FLAV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pestivirus strain ~~d32~~/~~00_hobi~~]]	+	[[Category: Pestivirus strain D32/00_HoBi]]
-	[[Category: Auer, B]]	+	[[Category: Auer B]]
-	[[Category: Brandstetter, H]]	+	[[Category: Brandstetter H]]
-	[[Category: Koll, M]]	+	[[Category: Koll M]]
-	[[Category: Schindler, S]]	+	[[Category: Schindler S]]
-	[[Category: Schneider, R]]	+	[[Category: Schneider R]]
-	[[Category: Sponring, M]]	+	[[Category: Sponring M]]
-	[[Category: Zogg, T]]	+	[[Category: Zogg T]]
-	~~[[Category: Auto-processing cysteine protease]]~~	+
-	~~[[Category: Auto-proteolysis]]~~	+
-	~~[[Category: Convergent evolution]]~~	+
-	~~[[Category: Host-pathogen interaction]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydroxide-dependent catalysis]]~~	+
-	~~[[Category: Immune modulation]]~~	+
-	~~[[Category: In cis- cleavage]]~~	+
-	~~[[Category: Pestivirus]]~~	+
-	~~[[Category: Viral protease~~]]	+

OCA at 05:45, 10 August 2022

OCA — Wed, 10 Aug 2022 05:45:11 GMT

←Older revision		Revision as of 05:45, 10 August 2022
Line 3:		Line 3:
	<StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zfn\|3zfn]], [[3zfo\|3zfo]], [[3zfq\|3zfq]], [[3zfr\|3zfr]], [[3zft\|3zft]], [[3zfu\|3zfu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zfn\|3zfn]], [[3zfo\|3zfo]], [[3zfq\|3zfq]], [[3zfr\|3zfr]], [[3zft\|3zft]], [[3zfu\|3zfu]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [~~http~~://pdbe.org/3zfp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [https://pdbe.org/3zfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [https://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Nonstructural protein\|Nonstructural protein]]	+	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:46, 17 July 2019

OCA — Wed, 17 Jul 2019 11:46:02 GMT

←Older revision		Revision as of 11:46, 17 July 2019
Line 1:		Line 1:

	==Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag==		==Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag==
-	<StructureSection load='3zfp' size='340' side='right' caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>	+	<StructureSection load='3zfp' size='340' side='right'caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pestivirus strain d32/00_hobi]]		[[Category: Pestivirus strain d32/00_hobi]]
	[[Category: Auer, B]]		[[Category: Auer, B]]

OCA at 16:56, 5 August 2016

OCA — Fri, 05 Aug 2016 16:56:52 GMT

←Older revision		Revision as of 16:56, 5 August 2016
Line 1:		Line 1:
-	{{STRUCTURE_3zfp\| PDB=3zfp \| SCENE= }}
-	===Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag===
-	{{ABSTRACT_PUBMED_23643950}}

-	==~~About this Structure~~==	+	==Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag==
-	[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA].	+	<StructureSection load='3zfp' size='340' side='right' caption='[[3zfp]], [[Resolution\|resolution]] 1.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zfn\|3zfn]], [[3zfo\|3zfo]], [[3zfq\|3zfq]], [[3zfr\|3zfr]], [[3zft\|3zft]], [[3zfu\|3zfu]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfp OCA], [http://pdbe.org/3zfp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zfp RCSB], [http://www.ebi.ac.uk/pdbsum/3zfp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfp ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Npro is a key effector protein of pestiviruses such as bovine viral diarrhea virus and abolishes host cell antiviral defense mechanisms. Synthesized as the N-terminal part of the viral polyprotein, Npro releases itself via an autoproteolytic cleavage, triggering its immunological functions. However, the mechanisms of its proteolytic action and its immune escape were unclear. Here, we present the crystal structures of Npro to 1.25 A resolution. Structures of pre- and postcleavage intermediates identify three catalytically relevant elements. The trapping of the putative catalytic water reveals its distinct roles as a base, acid, and nucleophile. The presentation of the substrate further explains the enigmatic latency of the protease, ensuring a single in cis cleavage. Additionally, we identified a zinc-free, disulfide-linked conformation of the TRASH motif, an interaction hub of immune factors. The structure opens additional opportunities in utilizing Npro as an autocleaving fusion protein and as a pharmaceutical target.

-	~~==Reference==~~	+	Crystal Structures of the Viral Protease Npro Imply Distinct Roles for the Catalytic Water in Catalysis.,Zogg T, Sponring M, Schindler S, Koll M, Schneider R, Brandstetter H, Auer B Structure. 2013 Apr 30. pii: S0969-2126(13)00112-3. doi:, 10.1016/j.str.2013.04.003. PMID:23643950<ref>PMID:23643950</ref>
-	<ref ~~group="xtra"~~>PMID:~~023643950~~</ref><~~references group~~="~~xtra~~"/><references/>	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3zfp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nonstructural protein\|Nonstructural protein]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pestivirus strain d32/00_hobi]]		[[Category: Pestivirus strain d32/00_hobi]]
-	[[Category: Auer, B.]]	+	[[Category: Auer, B]]
-	[[Category: Brandstetter, H.]]	+	[[Category: Brandstetter, H]]
-	[[Category: Koll, M.]]	+	[[Category: Koll, M]]
-	[[Category: Schindler, S.]]	+	[[Category: Schindler, S]]
-	[[Category: Schneider, R.]]	+	[[Category: Schneider, R]]
-	[[Category: Sponring, M.]]	+	[[Category: Sponring, M]]
-	[[Category: Zogg, T.]]	+	[[Category: Zogg, T]]
	[[Category: Auto-processing cysteine protease]]		[[Category: Auto-processing cysteine protease]]
	[[Category: Auto-proteolysis]]		[[Category: Auto-proteolysis]]

OCA at 07:42, 20 June 2013

OCA — Thu, 20 Jun 2013 07:42:23 GMT

←Older revision		Revision as of 07:42, 20 June 2013
Line 5:		Line 5:
	==About this Structure==		==About this Structure==
	[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA].		[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023643950</ref><references group="xtra"/><references/>
	[[Category: Pestivirus strain d32/00_hobi]]		[[Category: Pestivirus strain d32/00_hobi]]
	[[Category: Auer, B.]]		[[Category: Auer, B.]]

OCA at 07:17, 16 May 2013

OCA — Thu, 16 May 2013 07:17:42 GMT

←Older revision		Revision as of 07:17, 16 May 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3zfp\| PDB=3zfp \| SCENE= }}
		+	===Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag===
		+	{{ABSTRACT_PUBMED_23643950}}

-	~~The entry~~ 3zfp is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[3zfp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pestivirus_strain_d32/00_hobi Pestivirus strain d32/00_hobi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFP OCA].
-	~~Authors~~: ~~Zogg~~, T., ~~Sponring~~, M., Schindler, S.~~, Koll, M.,~~ Schneider, R., ~~Brandstetter, H~~., ~~Auer, B~~.	+	[[Category: Pestivirus strain d32/00_hobi]]
-		+	[[Category: Auer, B.]]
-	~~Description~~: ~~Crystal structure of product~~-~~like, processed N~~-~~terminal protease Npro with internal His~~-~~Tag~~	+	[[Category: Brandstetter, H.]]
		+	[[Category: Koll, M.]]
		+	[[Category: Schindler, S.]]
		+	[[Category: Schneider, R.]]
		+	[[Category: Sponring, M.]]
		+	[[Category: Zogg, T.]]
		+	[[Category: Auto-processing cysteine protease]]
		+	[[Category: Auto-proteolysis]]
		+	[[Category: Convergent evolution]]
		+	[[Category: Host-pathogen interaction]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydroxide-dependent catalysis]]
		+	[[Category: Immune modulation]]
		+	[[Category: In cis- cleavage]]
		+	[[Category: Pestivirus]]
		+	[[Category: Viral protease]]

OCA at 08:38, 2 January 2013

OCA — Wed, 02 Jan 2013 08:38:40 GMT

←Older revision		Revision as of 08:38, 2 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zfp is ON HOLD	+	The entry 3zfp is ON HOLD until Paper Publication

	Authors: Zogg, T., Sponring, M., Schindler, S., Koll, M., Schneider, R., Brandstetter, H., Auer, B.		Authors: Zogg, T., Sponring, M., Schindler, S., Koll, M., Schneider, R., Brandstetter, H., Auer, B.

	Description: Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag		Description: Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag

OCA: Protected "3zfp" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2012 10:49:48 GMT

Protected "3zfp" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:49, 19 December 2012

OCA: New page: '''Unreleased structure''' The entry 3zfp is ON HOLD Authors: Zogg, T., Sponring, M., Schindler, S., Koll, M., Schneider, R., Brandstetter, H., Auer, B. Description: Crystal structure ...

OCA — Wed, 19 Dec 2012 10:49:47 GMT

New page: '''Unreleased structure''' The entry 3zfp is ON HOLD Authors: Zogg, T., Sponring, M., Schindler, S., Koll, M., Schneider, R., Brandstetter, H., Auer, B. Description: Crystal structure ...

New page

'''Unreleased structure'''

The entry 3zfp is ON HOLD

Authors: Zogg, T., Sponring, M., Schindler, S., Koll, M., Schneider, R., Brandstetter, H., Auer, B.

Description: Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag