3zg1 - Revision history

OCA at 11:01, 20 December 2023

OCA — Wed, 20 Dec 2023 11:01:14 GMT

←Older revision		Revision as of 11:01, 20 December 2023
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	<StructureSection load='3zg1' size='340' side='right'caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3zg1' size='340' side='right'caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cupmc Cupmc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [https://pdbe.org/3zg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [https://www.ebi.ac.uk/pdbsum/3zg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zg1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [https://pdbe.org/3zg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [https://www.ebi.ac.uk/pdbsum/3zg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zg1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CNRR_RALME CNRR_RALME]~~] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.	+	[https://www.uniprot.org/uniprot/CNRR_CUPMC CNRR_CUPMC] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.<ref>PMID:10671463</ref> <ref>PMID:10671464</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cupmc~~]]	+	[[Category: Cupriavidus metallidurans CH34]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coves, J]]	+	[[Category: Coves J]]
-	[[Category: Girard, E]]	+	[[Category: Girard E]]
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Sensor protein]]~~	+
-	~~[[Category: Signal transduction~~]]	+

OCA at 05:45, 10 August 2022

OCA — Wed, 10 Aug 2022 05:45:34 GMT

←Older revision		Revision as of 05:45, 10 August 2022
Line 1:		Line 1:

	==NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS==		==NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS==
-	<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3zg1' size='340' side='right'caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [~~http~~://pdbe.org/3zg1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zg1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zg1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [https://pdbe.org/3zg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [https://www.ebi.ac.uk/pdbsum/3zg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zg1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.	+	[[https://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Cupmc]]		[[Category: Cupmc]]
		+	[[Category: Large Structures]]
	[[Category: Coves, J]]		[[Category: Coves, J]]
	[[Category: Girard, E]]		[[Category: Girard, E]]

OCA at 16:31, 27 February 2019

OCA — Wed, 27 Feb 2019 16:31:44 GMT

←Older revision		Revision as of 16:31, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cupriavidus metallidurans ch34~~]]	+	[[Category: Cupmc]]
	[[Category: Coves, J]]		[[Category: Coves, J]]
	[[Category: Girard, E]]		[[Category: Girard, E]]

OCA at 13:19, 5 August 2016

OCA — Fri, 05 Aug 2016 13:19:52 GMT

←Older revision		Revision as of 13:19, 5 August 2016
Line 1:		Line 1:
		+
	==NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS==		==NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS==
	<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [http://www.ebi.ac.uk/pdbsum/3zg1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [http://pdbe.org/3zg1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [http://www.ebi.ac.uk/pdbsum/3zg1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zg1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zg1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:48, 25 December 2014

OCA — Thu, 25 Dec 2014 02:48:40 GMT

←Older revision		Revision as of 02:48, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [http://www.ebi.ac.uk/pdbsum/3zg1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [http://www.ebi.ac.uk/pdbsum/3zg1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:12, 21 December 2014

OCA — Sun, 21 Dec 2014 09:12:43 GMT

←Older revision		Revision as of 09:12, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3zg1\| PDB=3zg1 \| SCENE= }}~~	+	==NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS==
-	===NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS===	+	<StructureSection load='3zg1' size='340' side='right' caption='[[3zg1]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24154823}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZG1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y39\|2y39]], [[2y3b\|2y3b]], [[2y3d\|2y3d]], [[2y3g\|2y3g]], [[2y3h\|2y3h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zg1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zg1 RCSB], [http://www.ebi.ac.uk/pdbsum/3zg1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	When CnrX, the periplasmic sensor protein in the CnrYXH transmembrane signal transduction complex of Cupriavidus metallidurans CH34, binds the cognate metal ions Ni(ii) or Co(ii), the ECF-type sigma factor CnrH is made available in the cytoplasm for the RNA-polymerase to initiate transcription at the cnrYp and cnrCp promoters. Ni(ii) or Co(ii) are sensed by a metal-binding site with a N3O2S coordination sphere with octahedral geometry, where S stands for the thioether sulfur of the only methionine (Met123) residue of CnrX. The M123A-CnrX derivative has dramatically reduced signal propagation in response to metal sensing while the X-ray structure of Ni-bound M123A-CnrXs showed that the metal-binding site was not affected by the mutation. Ni(ii) remained six-coordinate in M123A-CnrXs, with a water molecule replacing the sulfur as the sixth ligand. H32A-CnrXs, the soluble model of the wild-type membrane-anchored CnrX, was compared to the double mutants H32A-M123A-CnrXs and H32A-M123C-CnrXs to spectroscopically evaluate the role of this unique ligand in the binding site of Ni or Co. The Co- and Ni-bound forms of the protein display unusually blue-shifted visible spectra. TD-DFT calculations using structure-based models allowed identification and assignment of the electronic transitions of Co-bound form of the protein and its M123A derivative. Among them, the signature of the S-Co transition is distinguishable in the shoulder at 530 nm. In vitro affinity measurements point out the crucial role of Met123 in the selectivity for Ni or Co, and in vivo data support the conclusion that Met123 is a trigger of the signal transduction.

-	~~==Function==~~	+	Metal sensing and signal transduction by CnrX from Cupriavidus metallidurans CH34: role of the only methionine assessed by a functional, spectroscopic, and theoretical study.,Trepreau J, Grosse C, Mouesca JM, Sarret G, Girard E, Petit-Haertlein I, Kuennemann S, Desbourdes C, de Rosny E, Maillard AP, Nies DH, Coves J Metallomics. 2013 Oct 23. PMID:24154823<ref>PMID:24154823</ref>
-	~~[[http://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY~~ and ~~CrnR (~~CnrX~~) are needed for nickel induction of CnrH (PubMed~~:~~10671463)~~. ~~Has been suggested (PubMed:10671464) to bind nickel~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3zg1]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024154823</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cupriavidus metallidurans ch34]]		[[Category: Cupriavidus metallidurans ch34]]
-	[[Category: Coves, J.]]	+	[[Category: Coves, J]]
-	[[Category: Girard, E.]]	+	[[Category: Girard, E]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Sensor protein]]		[[Category: Sensor protein]]
	[[Category: Signal transduction]]		[[Category: Signal transduction]]

OCA at 05:38, 6 November 2013

OCA — Wed, 06 Nov 2013 05:38:34 GMT

←Older revision		Revision as of 05:38, 6 November 2013
Line 1:		Line 1:
	{{STRUCTURE_3zg1\| PDB=3zg1 \| SCENE= }}		{{STRUCTURE_3zg1\| PDB=3zg1 \| SCENE= }}
	===NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS===		===NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS===
		+	{{ABSTRACT_PUBMED_24154823}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA].		[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024154823</ref><references group="xtra"/><references/>
	[[Category: Cupriavidus metallidurans ch34]]		[[Category: Cupriavidus metallidurans ch34]]
	[[Category: Coves, J.]]		[[Category: Coves, J.]]

OCA at 06:50, 23 October 2013

OCA — Wed, 23 Oct 2013 06:50:50 GMT

←Older revision		Revision as of 06:50, 23 October 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3zg1\| PDB=3zg1 \| SCENE= }}
		+	===NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS===

-	~~The entry 3zg1~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.

-	~~Authors~~: ~~Girard, E~~., Coves, J.	+	==About this Structure==
-		+	[[3zg1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZG1 OCA].
-	~~Description~~: ~~NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS~~	+	[[Category: Cupriavidus metallidurans ch34]]
		+	[[Category: Coves, J.]]
		+	[[Category: Girard, E.]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Sensor protein]]
		+	[[Category: Signal transduction]]

OCA at 08:39, 2 January 2013

OCA — Wed, 02 Jan 2013 08:39:00 GMT

←Older revision		Revision as of 08:39, 2 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zg1 is ON HOLD	+	The entry 3zg1 is ON HOLD until Paper Publication

	Authors: Girard, E., Coves, J.		Authors: Girard, E., Coves, J.

	Description: NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS		Description: NI-BOUND FORM OF M123A MUTANT OF CUPRIAVIDUS METALLIDURANS CH34 CNRXS

OCA: Protected "3zg1" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2012 10:50:10 GMT

Protected "3zg1" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:50, 19 December 2012