3zit - Revision history

OCA at 11:02, 20 December 2023

OCA — Wed, 20 Dec 2023 11:02:42 GMT

←Older revision		Revision as of 11:02, 20 December 2023
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	<StructureSection load='3zit' size='340' side='right'caption='[[3zit]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='3zit' size='340' side='right'caption='[[3zit]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_14579 Atcc 14579~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~3zij~~\|~~3zij~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.18Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zit OCA], [https://pdbe.org/3zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zit RCSB], [https://www.ebi.ac.uk/pdbsum/3zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zit OCA], [https://pdbe.org/3zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zit RCSB], [https://www.ebi.ac.uk/pdbsum/3zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zit ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q819J1_BACCR Q819J1_BACCR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 14579~~]]	+	[[Category: Bacillus cereus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andersson~~, K K~~]]	+	[[Category: Andersson KK]]
-	[[Category: Hammerstad, M]]	+	[[Category: Hammerstad M]]
-	[[Category: Rohr~~, A K]]~~	+	[[Category: Rohr AK]]
-	~~[[Category: Glutaredoxin]]~~	+
-	~~[[Category: Grx]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Trx~~]]	+

OCA at 05:47, 10 August 2022

OCA — Wed, 10 Aug 2022 05:47:55 GMT

←Older revision		Revision as of 05:47, 10 August 2022
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	==Crystal structure of the thioredoxin-like protein BC3987 mutant T8A==		==Crystal structure of the thioredoxin-like protein BC3987 mutant T8A==
-	<StructureSection load='3zit' size='340' side='right' caption='[[3zit]], [[Resolution\|resolution]] 1.18Å' scene=''>	+	<StructureSection load='3zit' size='340' side='right'caption='[[3zit]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zit]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIT FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zij\|3zij]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zij\|3zij]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zit OCA], [~~http~~://pdbe.org/3zit PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zit RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zit PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zit ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zit OCA], [https://pdbe.org/3zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zit RCSB], [https://www.ebi.ac.uk/pdbsum/3zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zit ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Thioredoxin\|Thioredoxin]]	+	*[[Thioredoxin 3D structures\|Thioredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 14579]]		[[Category: Atcc 14579]]
		+	[[Category: Large Structures]]
	[[Category: Andersson, K K]]		[[Category: Andersson, K K]]
	[[Category: Hammerstad, M]]		[[Category: Hammerstad, M]]

OCA at 16:27, 4 August 2016

OCA — Thu, 04 Aug 2016 16:27:19 GMT

←Older revision		Revision as of 16:27, 4 August 2016
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-	{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}
-	===Crystal structure of the thioredoxin-like protein BC3987 mutant T8A===
-	{{ABSTRACT_PUBMED_23936007}}

-	==~~About this Structure~~==	+	==Crystal structure of the thioredoxin-like protein BC3987 mutant T8A==
-	[[3zit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_cereus Bacillus cereus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA].	+	<StructureSection load='3zit' size='340' side='right' caption='[[3zit]], [[Resolution\|resolution]] 1.18Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZIT FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zij\|3zij]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zit OCA], [http://pdbe.org/3zit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zit RCSB], [http://www.ebi.ac.uk/pdbsum/3zit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zit ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thioredoxin-like proteins contain a characteristic C-x-x-C active site motif and are involved in a large number of biological processes ranging from electron transfer, cellular redox level maintenance, and regulation of cellular processes. The mechanism for deprotonation of the buried C-terminal active site cysteine in thioredoxin, necessary for dissociation of the mixed-disulfide intermediate that occurs under thiol/disulfide mediated electron transfer, is not well understood for all thioredoxin superfamily members. Here we have characterized a 8.7 kD thioredoxin (BC3987) from Bacillus cereus that unlike the typical thioredoxin appears to use the conserved Thr8 side chain near the unusual C-P-P-C active site to increase enzymatic activity by forming a hydrogen bond to the buried cysteine. Our hypothesis is based on biochemical assays and thiolate pKa titrations where the wild type and T8A mutant are compared, phylogenetic analysis of related thioredoxins, and QM/MM calculations with the BC3987 crystal structure as a precursor for modeling of reduced active sites. We suggest that our model applies to other thioredoxin subclasses with similar active site arrangements.

-	~~==Reference==~~	+	Tuning of thioredoxin redox properties by intramolecular hydrogen bonds.,Rohr AK, Hammerstad M, Andersson KK PLoS One. 2013 Jul 23;8(7):e69411. doi: 10.1371/journal.pone.0069411. Print 2013. PMID:23936007<ref>PMID:23936007</ref>
-	<ref ~~group="xtra"~~>PMID:~~023936007~~</ref><~~references group~~="~~xtra~~"/><references/>	+
-	[[Category: ~~Bacillus cereus~~]]	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: Andersson, K K.]]	+	</div>
-	[[Category: Hammerstad, M.]]	+	<div class="pdbe-citations 3zit" style="background-color:#fffaf0;"></div>
-	[[Category: Rohr, A K.]]	+
		+	==See Also==
		+	*[[Thioredoxin\|Thioredoxin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 14579]]
		+	[[Category: Andersson, K K]]
		+	[[Category: Hammerstad, M]]
		+	[[Category: Rohr, A K]]
	[[Category: Glutaredoxin]]		[[Category: Glutaredoxin]]
	[[Category: Grx]]		[[Category: Grx]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Trx]]		[[Category: Trx]]

OCA at 05:20, 28 August 2013

OCA — Wed, 28 Aug 2013 05:20:37 GMT

←Older revision		Revision as of 05:20, 28 August 2013
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-	[[Image:3zit.png\|left\|200px]]
-
	{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}		{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}
-
	===Crystal structure of the thioredoxin-like protein BC3987 mutant T8A===		===Crystal structure of the thioredoxin-like protein BC3987 mutant T8A===
-		+	{{ABSTRACT_PUBMED_23936007}}

	==About this Structure==		==About this Structure==
	[[3zit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA].		[[3zit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023936007</ref><references group="xtra"/><references/>
	[[Category: Bacillus cereus]]		[[Category: Bacillus cereus]]
	[[Category: Andersson, K K.]]		[[Category: Andersson, K K.]]

OCA at 12:48, 30 January 2013

OCA — Wed, 30 Jan 2013 12:48:14 GMT

←Older revision		Revision as of 12:48, 30 January 2013
Line 1:		Line 1:
-	[[Image:3zit.~~jpg~~\|left\|200px]]	+	[[Image:3zit.png\|left\|200px]]

	{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}		{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}

OCA at 11:55, 23 January 2013

OCA — Wed, 23 Jan 2013 11:55:34 GMT

←Older revision		Revision as of 11:55, 23 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zit.jpg\|left\|200px]]

-	~~The entry~~ 3zit ~~is ON HOLD~~	+	{{STRUCTURE_3zit\| PDB=3zit \| SCENE= }}

-	~~Authors: Rohr, A.K., Hammerstad, M., Andersson, K.K.~~	+	===Crystal structure of the thioredoxin-like protein BC3987 mutant T8A===

-	~~Description: Crystal~~ structure ~~of the thioredoxin~~-~~like protein BC3987 mutant T8A~~	+
		+	==About this Structure==
		+	[[3zit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIT OCA].
		+	[[Category: Bacillus cereus]]
		+	[[Category: Andersson, K K.]]
		+	[[Category: Hammerstad, M.]]
		+	[[Category: Rohr, A K.]]
		+	[[Category: Glutaredoxin]]
		+	[[Category: Grx]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Trx]]

OCA: Protected "3zit" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2013 08:12:30 GMT

Protected "3zit" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:12, 16 January 2013

OCA: New page: '''Unreleased structure''' The entry 3zit is ON HOLD Authors: Rohr, A.K., Hammerstad, M., Andersson, K.K. Description: Crystal structure of the thioredoxin-like protein BC3987 mutant T...

OCA — Wed, 16 Jan 2013 08:12:29 GMT

New page: '''Unreleased structure''' The entry 3zit is ON HOLD Authors: Rohr, A.K., Hammerstad, M., Andersson, K.K. Description: Crystal structure of the thioredoxin-like protein BC3987 mutant T...

New page

'''Unreleased structure'''

The entry 3zit is ON HOLD

Authors: Rohr, A.K., Hammerstad, M., Andersson, K.K.

Description: Crystal structure of the thioredoxin-like protein BC3987 mutant T8A