3zki - Revision history

OCA at 11:04, 20 December 2023

OCA — Wed, 20 Dec 2023 11:04:25 GMT

←Older revision		Revision as of 11:04, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zki' size='340' side='right'caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3zki' size='340' side='right'caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [https://pdbe.org/3zki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [https://www.ebi.ac.uk/pdbsum/3zki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zki ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [https://pdbe.org/3zki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [https://www.ebi.ac.uk/pdbsum/3zki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zki ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>	+	[https://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Memapsin 1]]~~	+	[[Category: Banner DW]]
-	[[Category: Banner~~, D W~~]]	+	[[Category: Benz J]]
-	[[Category: Benz, J]]	+	[[Category: Kuglstatter A]]
-	[[Category: Kuglstatter, A]]	+	[[Category: Ruf A]]
-	[[Category: Ruf, A]]	+	[[Category: Stihle M]]
-	[[Category: Stihle, M]]	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 05:49, 10 August 2022

OCA — Wed, 10 Aug 2022 05:49:41 GMT

←Older revision		Revision as of 05:49, 10 August 2022
Line 1:		Line 1:

	==BACE2 MUTANT STRUCTURE WITH LIGAND==		==BACE2 MUTANT STRUCTURE WITH LIGAND==
-	<StructureSection load='3zki' size='340' side='right' caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='3zki' size='340' side='right'caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [~~http~~://pdbe.org/3zki PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zki PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zki ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [https://pdbe.org/3zki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [https://www.ebi.ac.uk/pdbsum/3zki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zki ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>	+	[[https://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Beta secretase\|Beta secretase]]	+	*[[Beta secretase 3D structures\|Beta secretase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Memapsin 1]]		[[Category: Memapsin 1]]
	[[Category: Banner, D W]]		[[Category: Banner, D W]]

OCA at 07:37, 5 August 2016

OCA — Fri, 05 Aug 2016 07:37:18 GMT

←Older revision		Revision as of 07:37, 5 August 2016
Line 1:		Line 1:
		+
	==BACE2 MUTANT STRUCTURE WITH LIGAND==		==BACE2 MUTANT STRUCTURE WITH LIGAND==
	<StructureSection load='3zki' size='340' side='right' caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3zki' size='340' side='right' caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [http://www.ebi.ac.uk/pdbsum/3zki PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [http://pdbe.org/3zki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [http://www.ebi.ac.uk/pdbsum/3zki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zki ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zki" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Memapsin 1]]		[[Category: Memapsin 1]]
	[[Category: Banner, D W]]		[[Category: Banner, D W]]

OCA at 16:57, 25 December 2014

OCA — Thu, 25 Dec 2014 16:57:17 GMT

←Older revision		Revision as of 16:57, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [http://www.ebi.ac.uk/pdbsum/3zki PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [http://www.ebi.ac.uk/pdbsum/3zki PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:56, 21 December 2014

OCA — Sun, 21 Dec 2014 09:56:29 GMT

←Older revision		Revision as of 09:56, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3zki~~\| ~~PDB~~=3zki \| ~~SCENE~~= }}	+	==BACE2 MUTANT STRUCTURE WITH LIGAND==
-	===~~BACE2 MUTANT STRUCTURE WITH LIGAND~~===	+	<StructureSection load='3zki' size='340' side='right' caption='[[3zki]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23695257}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zki]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZKI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WZV:5-(2,2,2-TRIFLUORO-ETHOXY)-PYRIDINE-2-CARBOXYLIC+ACID+[3-((S)-2-AMINO-1,4-DIMETHYL-6-OXO-1,4,5,6-TETRAHYDRO-PYRIMIDIN-4-YL)-PHENYL]-AMIDE'>WZV</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zkg\|3zkg]], [[3zkm\|3zkm]], [[3zkn\|3zkn]], [[3zkq\|3zkq]], [[3zkx\|3zkx]], [[3zl7\|3zl7]], [[3zov\|3zov]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Memapsin_1 Memapsin 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.45 3.4.23.45] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zki OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zki RCSB], [http://www.ebi.ac.uk/pdbsum/3zki PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The aspartic protease BACE2 is responsible for the shedding of the transmembrane protein Tmem27 from the surface of pancreatic beta-cells, which leads to inactivation of the beta-cell proliferating activity of Tmem27. This role of BACE2 in the control of beta-cell maintenance suggests BACE2 as a drug target for diabetes. Inhibition of BACE2 has recently been shown to lead to improved control of glucose homeostasis and to increased insulin levels in insulin-resistant mice. BACE2 has 52% sequence identity to the well studied Alzheimer's disease target enzyme beta-secretase (BACE1). High-resolution BACE2 structures would contribute significantly to the investigation of this enzyme as either a drug target or anti-target. Surface mutagenesis, BACE2-binding antibody Fab fragments, single-domain camelid antibody VHH fragments (Xaperones) and Fyn-kinase-derived SH3 domains (Fynomers) were used as crystallization helpers to obtain the first high-resolution structures of BACE2. Eight crystal structures in six different packing environments define an ensemble of low-energy conformations available to the enzyme. Here, the different strategies used for raising and selecting BACE2 binders for cocrystallization are described and the crystallization success, crystal quality and the time and resources needed to obtain suitable crystals are compared.

-	~~==Function==~~	+	Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones.,Banner DW, Gsell B, Benz J, Bertschinger J, Burger D, Brack S, Cuppuleri S, Debulpaep M, Gast A, Grabulovski D, Hennig M, Hilpert H, Huber W, Kuglstatter A, Kusznir E, Laeremans T, Matile H, Miscenic C, Rufer AC, Schlatter D, Steyaert J, Stihle M, Thoma R, Weber M, Ruf A Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1124-37. doi:, 10.1107/S0907444913006574. Epub 2013 May 15. PMID:23695257<ref>PMID:23695257</ref>
-	~~[[http://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for~~ the ~~proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690~~ and ~~691~~, ~~leading to the generation~~ and ~~extracellular release of beta-cleaved soluble APP~~, ~~and a corresponding cell-associated~~ C-~~terminal fragment which is later released by gamma-secretase~~. ~~It has also been shown that it can cleave APP between residues 671 and 672~~.~~<ref>PMID:10591213<~~/~~ref> <ref>~~PMID:~~11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref>~~ <ref>PMID:~~16816112~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3zki]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023695257</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Beta secretase\|Beta secretase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Memapsin 1]]		[[Category: Memapsin 1]]
-	[[Category: Banner, D W.]]	+	[[Category: Banner, D W]]
-	[[Category: Benz, J.]]	+	[[Category: Benz, J]]
-	[[Category: Kuglstatter, A.]]	+	[[Category: Kuglstatter, A]]
-	[[Category: Ruf, A.]]	+	[[Category: Ruf, A]]
-	[[Category: Stihle, M.]]	+	[[Category: Stihle, M]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 16:03, 19 June 2013

OCA — Wed, 19 Jun 2013 16:03:07 GMT

←Older revision		Revision as of 16:03, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3zki\| PDB=3zki \| SCENE= }}
		+	===BACE2 MUTANT STRUCTURE WITH LIGAND===
		+	{{ABSTRACT_PUBMED_23695257}}

-	~~The entry 3zki~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/BACE2_HUMAN BACE2_HUMAN]] Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.<ref>PMID:10591213</ref> <ref>PMID:11083922</ref> <ref>PMID:15857888</ref> <ref>PMID:11423558</ref> <ref>PMID:16816112</ref>

-	~~Authors~~: ~~Banner, D~~.W.~~, Kuglstatter, A~~.~~, Benz, J~~.~~, Stihle, M~~.~~, Ruf, A~~.	+	==About this Structure==
		+	[[3zki]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKI OCA].

-	~~Description~~: ~~BACE2 MUTANT STRUCTURE WITH LIGAND~~	+	==Reference==
		+	<ref group="xtra">PMID:023695257</ref><references group="xtra"/><references/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Memapsin 1]]
		+	[[Category: Banner, D W.]]
		+	[[Category: Benz, J.]]
		+	[[Category: Kuglstatter, A.]]
		+	[[Category: Ruf, A.]]
		+	[[Category: Stihle, M.]]
		+	[[Category: Hydrolase]]

OCA at 16:03, 20 March 2013

OCA — Wed, 20 Mar 2013 16:03:12 GMT

←Older revision		Revision as of 16:03, 20 March 2013
Line 3:		Line 3:
	The entry 3zki is ON HOLD until Paper Publication		The entry 3zki is ON HOLD until Paper Publication

-	Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski, D., Gsell, B., Hilpert, H., Huber, W., Kusznir, E., Laeremans, T., Matile, H., Rufer, A., Schlatter, D., Steyeart, J., Stihle~~, M., Thoma, R., Weber~~, M., Ruf, A.	+	Authors: Banner, D.W., Kuglstatter, A., Benz, J., Stihle, M., Ruf, A.

	Description: BACE2 MUTANT STRUCTURE WITH LIGAND		Description: BACE2 MUTANT STRUCTURE WITH LIGAND

OCA at 13:12, 20 February 2013

OCA — Wed, 20 Feb 2013 13:12:13 GMT

←Older revision		Revision as of 13:12, 20 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zki is ON HOLD	+	The entry 3zki is ON HOLD until Paper Publication

	Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski, D., Gsell, B., Hilpert, H., Huber, W., Kusznir, E., Laeremans, T., Matile, H., Rufer, A., Schlatter, D., Steyeart, J., Stihle, M., Thoma, R., Weber, M., Ruf, A.		Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski, D., Gsell, B., Hilpert, H., Huber, W., Kusznir, E., Laeremans, T., Matile, H., Rufer, A., Schlatter, D., Steyeart, J., Stihle, M., Thoma, R., Weber, M., Ruf, A.

	Description: BACE2 MUTANT STRUCTURE WITH LIGAND		Description: BACE2 MUTANT STRUCTURE WITH LIGAND

OCA: Protected "3zki" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2013 11:49:31 GMT

Protected "3zki" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:49, 30 January 2013

OCA: New page: '''Unreleased structure''' The entry 3zki is ON HOLD Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski,...

OCA — Wed, 30 Jan 2013 11:49:29 GMT

New page: '''Unreleased structure''' The entry 3zki is ON HOLD Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski,...

New page

'''Unreleased structure'''

The entry 3zki is ON HOLD

Authors: Banner, D.W., Kuglstatter, A., Benz, J., Bertschinger, J., Burger, D., Cuppuleri, S., Debulpaep, M., Gast, A., Grabulovski, D., Gsell, B., Hilpert, H., Huber, W., Kusznir, E., Laeremans, T., Matile, H., Rufer, A., Schlatter, D., Steyeart, J., Stihle, M., Thoma, R., Weber, M., Ruf, A.

Description: BACE2 MUTANT STRUCTURE WITH LIGAND