3zqx - Revision history

OCA at 07:13, 1 May 2024

OCA — Wed, 01 May 2024 07:13:19 GMT

←Older revision		Revision as of 07:13, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='3zqx' size='340' side='right'caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3zqx' size='340' side='right'caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.04Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2ylk\|2ylk]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [https://pdbe.org/3zqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [https://www.ebi.ac.uk/pdbsum/3zqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [https://pdbe.org/3zqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [https://www.ebi.ac.uk/pdbsum/3zqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqx ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q59325_ACETH Q59325_ACETH]
-	The crystal structure of the family 3b carbohydrate-binding module (CBM3b) of the cellulosomal multimodular hydrolytic enzyme cellobiohydrolase 9A (Cbh9A) from Clostridium thermocellum has been determined. Cbh9A CBM3b crystallized in space group P4(1) with four molecules in the asymmetric unit and diffracted to a resolution of 2.20 A using synchrotron radiation. The structure was determined by molecular replacement using C. thermocellum Cel9V CBM3b' (PDB entry 2wnx) as a model. The C. thermocellum Cbh9A CBM3b molecule forms a nine-stranded antiparallel beta-sandwich similar to other family 3 carbohydrate-binding modules (CBMs). It has a short planar array of two aromatic residues that are assumed to bind cellulose, yet it lacks the ability to bind cellulose. The molecule contains a shallow groove of unknown function that characterizes other family 3 CBMs with high sequence homology. In addition, it contains a calcium-binding site formed by a group of amino-acid residues that are highly conserved in similar structures. After determination of the three-dimensional structure of Cbh9A CBM3b, the site-specific N126W mutant was produced with the intention of enhancing the cellulose-binding ability of the CBM. Cbh9A CBM3b(N126W) crystallized in space group P4(1)2(1)2, with one molecule in the asymmetric unit. The crystals diffracted to 1.04 A resolution using synchrotron radiation. The structure of Cbh9A CBM3b(N126W) revealed incorporation of the mutated Trp126 into the putative cellulose-binding strip of residues. Cellulose-binding experiments demonstrated the ability of Cbh9A CBM3b(N126W) to bind cellulose owing to the mutation. This is the first report of the engineered conversion of a non-cellulose-binding CBM3 to a binding CBM3 by site-directed mutagenesis. The three-dimensional structure of Cbh9A CBM3b(N126W) provided a structural correlation with cellulose-binding ability, revealing a longer planar array of definitive cellulose-binding residues.	+
-		+
-	A single mutation reforms the binding activity of an adhesion-deficient family 3 carbohydrate-binding module.,Yaniv O, Petkun S, Shimon LJ, Bayer EA, Lamed R, Frolow F Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):~~819-28. Epub 2012 Jun 15. PMID:22751667<ref>PMID:22751667<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3zqx" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acetivibrio thermocellus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bayer~~, E A~~]]	+	[[Category: Bayer EA]]
-	[[Category: Frolow, F]]	+	[[Category: Frolow F]]
-	[[Category: Lamed, R]]	+	[[Category: Lamed R]]
-	[[Category: Petkun, S]]	+	[[Category: Petkun S]]
-	[[Category: Shimon~~, L J.W~~]]	+	[[Category: Shimon LJW]]
-	[[Category: Yaniv, O]]	+	[[Category: Yaniv O]]
-	~~[[Category: Cellulose binding protein]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:11, 18 August 2022

OCA — Thu, 18 Aug 2022 07:11:10 GMT

←Older revision		Revision as of 07:11, 18 August 2022
Line 1:		Line 1:

	==Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum==		==Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum==
-	<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>	+	<StructureSection load='3zqx' size='340' side='right'caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ylk\|2ylk]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [~~http~~://pdbe.org/3zqx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zqx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [https://pdbe.org/3zqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [https://www.ebi.ac.uk/pdbsum/3zqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Frolow, F]]		[[Category: Frolow, F]]

OCA at 16:21, 27 February 2019

OCA — Wed, 27 Feb 2019 16:21:09 GMT

←Older revision		Revision as of 16:21, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_27405 Atcc 27405~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 27405]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Frolow, F]]		[[Category: Frolow, F]]

OCA at 13:21, 11 August 2016

OCA — Thu, 11 Aug 2016 13:21:52 GMT

←Older revision		Revision as of 13:21, 11 August 2016
Line 1:		Line 1:
		+
	==Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum==		==Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum==
	<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_thermocellum Clostridium thermocellum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27405 Atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-~~cellobiosidase~~ Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [http://www.ebi.ac.uk/pdbsum/3zqx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [http://pdbe.org/3zqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [http://www.ebi.ac.uk/pdbsum/3zqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zqx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cellulose 1,4-beta-cellobiosidase]]~~	+	[[Category: Atcc 27405]]
-	~~[[Category: Clostridium thermocellum~~]]	+
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Frolow, F]]		[[Category: Frolow, F]]

OCA at 10:19, 4 January 2015

OCA — Sun, 04 Jan 2015 10:19:10 GMT

←Older revision		Revision as of 10:19, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~3zqx.~~png~~\|~~left~~\|~~200px~~]]	+	==Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum==
		+	<StructureSection load='3zqx' size='340' side='right' caption='[[3zqx]], [[Resolution\|resolution]] 1.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ylk\|2ylk]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zqx RCSB], [http://www.ebi.ac.uk/pdbsum/3zqx PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the family 3b carbohydrate-binding module (CBM3b) of the cellulosomal multimodular hydrolytic enzyme cellobiohydrolase 9A (Cbh9A) from Clostridium thermocellum has been determined. Cbh9A CBM3b crystallized in space group P4(1) with four molecules in the asymmetric unit and diffracted to a resolution of 2.20 A using synchrotron radiation. The structure was determined by molecular replacement using C. thermocellum Cel9V CBM3b' (PDB entry 2wnx) as a model. The C. thermocellum Cbh9A CBM3b molecule forms a nine-stranded antiparallel beta-sandwich similar to other family 3 carbohydrate-binding modules (CBMs). It has a short planar array of two aromatic residues that are assumed to bind cellulose, yet it lacks the ability to bind cellulose. The molecule contains a shallow groove of unknown function that characterizes other family 3 CBMs with high sequence homology. In addition, it contains a calcium-binding site formed by a group of amino-acid residues that are highly conserved in similar structures. After determination of the three-dimensional structure of Cbh9A CBM3b, the site-specific N126W mutant was produced with the intention of enhancing the cellulose-binding ability of the CBM. Cbh9A CBM3b(N126W) crystallized in space group P4(1)2(1)2, with one molecule in the asymmetric unit. The crystals diffracted to 1.04 A resolution using synchrotron radiation. The structure of Cbh9A CBM3b(N126W) revealed incorporation of the mutated Trp126 into the putative cellulose-binding strip of residues. Cellulose-binding experiments demonstrated the ability of Cbh9A CBM3b(N126W) to bind cellulose owing to the mutation. This is the first report of the engineered conversion of a non-cellulose-binding CBM3 to a binding CBM3 by site-directed mutagenesis. The three-dimensional structure of Cbh9A CBM3b(N126W) provided a structural correlation with cellulose-binding ability, revealing a longer planar array of definitive cellulose-binding residues.

-	~~{{STRUCTURE_3zqx\| PDB=3zqx \| SCENE= }}~~	+	A single mutation reforms the binding activity of an adhesion-deficient family 3 carbohydrate-binding module.,Yaniv O, Petkun S, Shimon LJ, Bayer EA, Lamed R, Frolow F Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):819-28. Epub 2012 Jun 15. PMID:22751667<ref>PMID:22751667</ref>

-	~~===Carbohydrate-binding module CBM3b from~~ the ~~cellulosomal cellobiohydrolase 9A from Clostridium thermocellum===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22751667}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3zqx]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_thermocellum Clostridium thermocellum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022751667<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
-	[[Category: Bayer, E A.]]	+	[[Category: Bayer, E A]]
-	[[Category: Frolow, F.]]	+	[[Category: Frolow, F]]
-	[[Category: Lamed, R.]]	+	[[Category: Lamed, R]]
-	[[Category: Petkun, S.]]	+	[[Category: Petkun, S]]
-	[[Category: Shimon, L J.W.]]	+	[[Category: Shimon, L J.W]]
-	[[Category: Yaniv, O.]]	+	[[Category: Yaniv, O]]
	[[Category: Cellulose binding protein]]		[[Category: Cellulose binding protein]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:32, 11 July 2012

OCA — Wed, 11 Jul 2012 07:32:20 GMT

←Older revision		Revision as of 07:32, 11 July 2012
Line 5:		Line 5:
	===Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum===		===Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum===

		+	{{ABSTRACT_PUBMED_22751667}}

	==About this Structure==		==About this Structure==
	[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA].		[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022751667</ref><references group="xtra"/>
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]

OCA at 04:44, 27 June 2012

OCA — Wed, 27 Jun 2012 04:44:35 GMT

←Older revision		Revision as of 04:44, 27 June 2012
Line 1:		Line 1:
-	[[Image:3zqx.~~jpg~~\|left\|200px]]	+	[[Image:3zqx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3zqx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3zqx\| PDB=3zqx \| SCENE= }}		{{STRUCTURE_3zqx\| PDB=3zqx \| SCENE= }}

OCA at 09:24, 25 April 2012

OCA — Wed, 25 Apr 2012 09:24:24 GMT

←Older revision		Revision as of 09:24, 25 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zqx.jpg\|left\|200px]]

-	The ~~entry 3zqx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zqx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zqx\| PDB=3zqx \| SCENE= }}

-	~~Authors: Yaniv, O., Petkun, S., Shimon, L.J.W., Bayer, E.A., Lamed, R., Frolow, F.~~	+	===Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum===

-	~~Description~~: ~~Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A~~ from Clostridium thermocellum	+
		+	==About this Structure==
		+	[[3zqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQX OCA].
		+	[[Category: Cellulose 1,4-beta-cellobiosidase]]
		+	[[Category: Clostridium thermocellum]]
		+	[[Category: Bayer, E A.]]
		+	[[Category: Frolow, F.]]
		+	[[Category: Lamed, R.]]
		+	[[Category: Petkun, S.]]
		+	[[Category: Shimon, L J.W.]]
		+	[[Category: Yaniv, O.]]
		+	[[Category: Cellulose binding protein]]
		+	[[Category: Hydrolase]]

OCA at 18:31, 6 July 2011

OCA — Wed, 06 Jul 2011 18:31:44 GMT

←Older revision		Revision as of 18:31, 6 July 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zqx is ON HOLD	+	The entry 3zqx is ON HOLD until Paper Publication

	Authors: Yaniv, O., Petkun, S., Shimon, L.J.W., Bayer, E.A., Lamed, R., Frolow, F.		Authors: Yaniv, O., Petkun, S., Shimon, L.J.W., Bayer, E.A., Lamed, R., Frolow, F.

	Description: Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum		Description: Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum

OCA: Protected "3zqx" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2011 16:25:55 GMT

Protected "3zqx" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:25, 22 June 2011