3zs9 - Revision history

OCA at 11:11, 20 December 2023

OCA — Wed, 20 Dec 2023 11:11:26 GMT

←Older revision		Revision as of 11:11, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zs9' size='340' side='right'caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3zs9' size='340' side='right'caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.095Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2woj\|2woj]], [[3zs8\|3zs8]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [https://pdbe.org/3zs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [https://www.ebi.ac.uk/pdbsum/3zs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zs9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [https://pdbe.org/3zs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [https://www.ebi.ac.uk/pdbsum/3zs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zs9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[https://www.uniprot.org/uniprot/GET2_YEAST GET2_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.[HAMAP-Rule:MF_03114]<ref>PMID:10929718</ref> <ref>PMID:12586695</ref> <ref>PMID:12615994</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21719644</ref> <ref>PMID:21835666</ref>	+	[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arsenite-transporting ATPase]]
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bove, E]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Dobosz, M]]	+	[[Category: Bove E]]
-	[[Category: Hegde~~, R S~~]]	+	[[Category: Dobosz M]]
-	[[Category: Keenan~~, R J~~]]	+	[[Category: Hegde RS]]
-	[[Category: Mariappan, M]]	+	[[Category: Keenan RJ]]
-	[[Category: Mateja, A]]	+	[[Category: Mariappan M]]
-	~~[[Category: Hydrolase-transport protein complex]]~~	+	[[Category: Mateja A]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Targeting factor~~]]	+

OCA at 07:14, 18 August 2022

OCA — Thu, 18 Aug 2022 07:14:02 GMT

←Older revision		Revision as of 07:14, 18 August 2022
Line 1:		Line 1:

	==S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment==		==S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment==
-	<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3zs9' size='340' side='right'caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2woj\|2woj]], [[3zs8\|3zs8]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2woj\|2woj]], [[3zs8\|3zs8]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [~~http~~://pdbe.org/3zs9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zs9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zs9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [https://pdbe.org/3zs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [https://www.ebi.ac.uk/pdbsum/3zs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zs9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[~~http~~://www.uniprot.org/uniprot/GET2_YEAST GET2_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.[HAMAP-Rule:MF_03114]<ref>PMID:10929718</ref> <ref>PMID:12586695</ref> <ref>PMID:12615994</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21719644</ref> <ref>PMID:21835666</ref>	+	[[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[https://www.uniprot.org/uniprot/GET2_YEAST GET2_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.[HAMAP-Rule:MF_03114]<ref>PMID:10929718</ref> <ref>PMID:12586695</ref> <ref>PMID:12615994</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21719644</ref> <ref>PMID:21835666</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[ATPase\|ATPase]]	+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Bove, E]]		[[Category: Bove, E]]
	[[Category: Dobosz, M]]		[[Category: Dobosz, M]]

OCA at 17:02, 27 February 2019

OCA — Wed, 27 Feb 2019 17:02:03 GMT

←Older revision		Revision as of 17:02, 27 February 2019
Line 1:		Line 1:

-	==S. ~~CEREVISIAE GET3~~-ADP-~~ALF4~~- ~~COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT~~==	+	==S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment==
	<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 15:21, 5 August 2016

OCA — Fri, 05 Aug 2016 15:21:59 GMT

←Older revision		Revision as of 15:21, 5 August 2016
Line 1:		Line 1:
		+
	==S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT==		==S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT==
	<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2woj\|2woj]], [[3zs8\|3zs8]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2woj\|2woj]], [[3zs8\|3zs8]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zs9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [http://pdbe.org/3zs9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zs9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zs9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zs9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Bove, E]]		[[Category: Bove, E]]
	[[Category: Dobosz, M]]		[[Category: Dobosz, M]]

OCA at 19:53, 25 December 2014

OCA — Thu, 25 Dec 2014 19:53:26 GMT

←Older revision		Revision as of 19:53, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zs9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zs9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[http://www.uniprot.org/uniprot/GET2_YEAST GET2_YEAST]] Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.[HAMAP-Rule:MF_03114]<ref>PMID:10929718</ref> <ref>PMID:12586695</ref> <ref>PMID:12615994</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21719644</ref> <ref>PMID:21835666</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:42, 9 December 2014

OCA — Tue, 09 Dec 2014 17:42:03 GMT

←Older revision		Revision as of 17:42, 9 December 2014
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-	[[~~Image:~~3zs9.~~png~~\|~~left~~\|~~200px~~]]	+	==S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT==
		+	<StructureSection load='3zs9' size='340' side='right' caption='[[3zs9]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZS9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2woj\|2woj]], [[3zs8\|3zs8]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zs9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zs9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zs9 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) membrane proteins destined for the endoplasmic reticulum are chaperoned by cytosolic targeting factors that deliver them to a membrane receptor for insertion. Although a basic framework for TA protein recognition is now emerging, the decisive targeting and membrane insertion steps are not understood. Here we reconstitute the TA protein insertion cycle with purified components, present crystal structures of key complexes between these components and perform mutational analyses based on the structures. We show that a committed targeting complex, formed by a TA protein bound to the chaperone ATPase Get3, is initially recruited to the membrane through an interaction with Get2. Once the targeting complex has been recruited, Get1 interacts with Get3 to drive TA protein release in an ATPase-dependent reaction. After releasing its TA protein cargo, the now-vacant Get3 recycles back to the cytosol concomitant with ATP binding. This work provides a detailed structural and mechanistic framework for the minimal TA protein insertion cycle.

-	~~{{STRUCTURE_3zs9\| PDB=3zs9 \| SCENE= }}~~	+	The mechanism of membrane-associated steps in tail-anchored protein insertion.,Mariappan M, Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJ Nature. 2011 Aug 24;477(7362):61-6. doi: 10.1038/nature10362. PMID:21866104<ref>PMID:21866104</ref>

-	~~===S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21866104}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3zs9]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].~~	+

	==See Also==		==See Also==
	*[[ATPase\|ATPase]]		*[[ATPase\|ATPase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021866104<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Bove, E.]]	+	[[Category: Bove, E]]
-	[[Category: Dobosz, M.]]	+	[[Category: Dobosz, M]]
-	[[Category: Hegde, R S.]]	+	[[Category: Hegde, R S]]
-	[[Category: Keenan, R J.]]	+	[[Category: Keenan, R J]]
-	[[Category: Mariappan, M.]]	+	[[Category: Mariappan, M]]
-	[[Category: Mateja, A.]]	+	[[Category: Mateja, A]]
	[[Category: Hydrolase-transport protein complex]]		[[Category: Hydrolase-transport protein complex]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Targeting factor]]		[[Category: Targeting factor]]

OCA at 20:47, 20 October 2012

OCA — Sat, 20 Oct 2012 20:47:42 GMT

←Older revision		Revision as of 20:47, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3zs9]] is a 4 chain structure ~~of [[ATPase]]~~ with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].	+	[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].

	==See Also==		==See Also==

OCA at 11:38, 27 July 2012

OCA — Fri, 27 Jul 2012 11:38:37 GMT

←Older revision		Revision as of 11:38, 27 July 2012
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	[[Image:3zs9.png\|left\|200px]]		[[Image:3zs9.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3zs9\| PDB=3zs9 \| SCENE= }}		{{STRUCTURE_3zs9\| PDB=3zs9 \| SCENE= }}

	===S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT===		===S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21866104}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21866104 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21866104}}		{{ABSTRACT_PUBMED_21866104}}

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	==See Also==		==See Also==
-	*[[ATPase]]	+	*[[ATPase\|ATPase]]

	==Reference==		==Reference==

OCA at 10:51, 9 November 2011

OCA — Wed, 09 Nov 2011 10:51:16 GMT

←Older revision		Revision as of 10:51, 9 November 2011
Line 1:		Line 1:
-	[[Image:3zs9.~~jpg~~\|left\|200px]]	+	[[Image:3zs9.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].	+	[[3zs9]] is a 4 chain structure of [[ATPase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].
		+
		+	==See Also==
		+	*[[ATPase]]

	==Reference==		==Reference==

OCA at 06:10, 7 September 2011

OCA — Wed, 07 Sep 2011 06:10:53 GMT

←Older revision		Revision as of 06:10, 7 September 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3zs9.jpg\|left\|200px]]

-	The ~~entry 3zs9~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zs9", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zs9\| PDB=3zs9 \| SCENE= }}

-	~~Authors: Mariappan, M~~.~~, Mateja,~~ A~~., Dobosz, M., Bove, E., Hegde, R.S., Keenan, R.J.~~	+	===S. CEREVISIAE GET3-ADP-ALF4- COMPLEX WITH A CYTOSOLIC GET2 FRAGMENT===

-	~~Description~~: S. cerevisiae ~~Get3~~-~~ADP~~-~~AlF4~~-complex ~~with a cytosolic Get2 fragment~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21866104}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21866104 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21866104}}
		+
		+	==About this Structure==
		+	[[3zs9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZS9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021866104</ref><references group="xtra"/>
		+	[[Category: Arsenite-transporting ATPase]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Bove, E.]]
		+	[[Category: Dobosz, M.]]
		+	[[Category: Hegde, R S.]]
		+	[[Category: Keenan, R J.]]
		+	[[Category: Mariappan, M.]]
		+	[[Category: Mateja, A.]]
		+	[[Category: Hydrolase-transport protein complex]]
		+	[[Category: Membrane protein]]
		+	[[Category: Targeting factor]]