3zth - Revision history

OCA at 07:16, 18 August 2022

OCA — Thu, 18 Aug 2022 07:16:46 GMT

←Older revision		Revision as of 07:16, 18 August 2022
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	==Crystal structure of Stu0660 of Streptococcus thermophilus==		==Crystal structure of Stu0660 of Streptococcus thermophilus==
-	<StructureSection load='3zth' size='340' side='right' caption='[[3zth]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='3zth' size='340' side='right'caption='[[3zth]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zth]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strt2 Strt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZTH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strt2 Strt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZTH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zth OCA], [~~http~~://pdbe.org/3zth PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zth RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zth PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zth ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zth OCA], [https://pdbe.org/3zth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zth RCSB], [https://www.ebi.ac.uk/pdbsum/3zth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zth ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strt2]]		[[Category: Strt2]]
	[[Category: Lee, K H]]		[[Category: Lee, K H]]

OCA at 18:59, 10 December 2016

OCA — Sat, 10 Dec 2016 18:59:12 GMT

←Older revision		Revision as of 18:59, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of Stu0660 of Streptococcus thermophilus==		==Crystal structure of Stu0660 of Streptococcus thermophilus==
	<StructureSection load='3zth' size='340' side='right' caption='[[3zth]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3zth' size='340' side='right' caption='[[3zth]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_thermophilus_lmg_18311 Streptococcus thermophilus lmg 18311~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZTH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strt2 Strt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZTH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zth OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zth RCSB], [http://www.ebi.ac.uk/pdbsum/3zth PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zth OCA], [http://pdbe.org/3zth PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zth RCSB], [http://www.ebi.ac.uk/pdbsum/3zth PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zth ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zth" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptococcus thermophilus lmg 18311~~]]	+	[[Category: Strt2]]
	[[Category: Lee, K H]]		[[Category: Lee, K H]]
	[[Category: Oh, B H]]		[[Category: Oh, B H]]

OCA at 17:21, 9 December 2014

OCA — Tue, 09 Dec 2014 17:21:32 GMT

←Older revision		Revision as of 17:21, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3zth.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Stu0660 of Streptococcus thermophilus==
		+	<StructureSection load='3zth' size='340' side='right' caption='[[3zth]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_thermophilus_lmg_18311 Streptococcus thermophilus lmg 18311]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZTH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zth OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zth RCSB], [http://www.ebi.ac.uk/pdbsum/3zth PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many prokaryotic organisms acquire immunity against foreign genetic material by incorporating a short segment of foreign DNA called spacer into chromosomal loci, termed clustered regularly interspaced short palindromic repeats (CRISPRs). The encoded RNAs are processed into small fragments that guide the silencing of the invading genetic elements. The CRISPR-associated (Cas) proteins are the main executioners of these processes. Herein, we report the crystal structure of Stu0660 of Streptococcus thermophilus, a Cas protein involved in the acquisition of new spacers. By homotetramerization, Stu0660 forms a central channel which is decorated with basic amino acids and binds linear double-stranded DNA (dsDNA), but not circular dsDNA. Despite undetectably low sequence similarity, two N-terminal domains of Stu0660 are similar to the entire structure of an Enterococcus faecalis Csn2 protein, which also forms a homotetramer and binds dsDNA. Thus, this work identifies a previously unknown group of Stu0660-like Csn2 proteins ( approximately 350 residues), which are larger than the known canonical Csn2 proteins ( approximately 220 residues) by containing an extra C-terminal domain. The commonly present central channel in the two subgroups appears as a design to selectively interact with linear dsDNA. Proteins 2012;. (c) 2012 Wiley Periodicals, Inc.

-	~~{{STRUCTURE_3zth\| PDB=3zth \| SCENE= }}~~	+	Identification, structural, and biochemical characterization of a group of large Csn2 proteins involved in CRISPR-mediated bacterial immunity.,Lee KH, Lee SG, Eun Lee K, Jeon H, Robinson H, Oh BH Proteins. 2012 Jul 2. doi: 10.1002/prot.24138. PMID:22753072<ref>PMID:22753072</ref>

-	~~===Crystal structure~~ of ~~Stu0660 of Streptococcus thermophilus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22753072}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3zth]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_thermophilus_lmg_18311 Streptococcus thermophilus lmg 18311]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022753072<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptococcus thermophilus lmg 18311]]		[[Category: Streptococcus thermophilus lmg 18311]]
-	[[Category: Lee, K H.]]	+	[[Category: Lee, K H]]
-	[[Category: Oh, B H.]]	+	[[Category: Oh, B H]]
-	[[Category: Robinson, H.]]	+	[[Category: Robinson, H]]
	[[Category: Ca]]		[[Category: Ca]]
	[[Category: Crispr]]		[[Category: Crispr]]
	[[Category: Dna binding]]		[[Category: Dna binding]]

OCA at 09:32, 31 October 2012

OCA — Wed, 31 Oct 2012 09:32:45 GMT

←Older revision		Revision as of 09:32, 31 October 2012
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	==About this Structure==		==About this Structure==
-	[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_thermophilus~~ Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].	+	[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_thermophilus_lmg_18311 Streptococcus thermophilus lmg 18311]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:022753072</ref><references group="xtra"/>		<ref group="xtra">PMID:022753072</ref><references group="xtra"/>
-	[[Category: Streptococcus thermophilus]]	+	[[Category: Streptococcus thermophilus lmg 18311]]
	[[Category: Lee, K H.]]		[[Category: Lee, K H.]]
	[[Category: Oh, B H.]]		[[Category: Oh, B H.]]

OCA at 04:25, 8 August 2012

OCA — Wed, 08 Aug 2012 04:25:56 GMT

←Older revision		Revision as of 04:25, 8 August 2012
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-	[[Image:3zth.~~jpg~~\|left\|200px]]	+	[[Image:3zth.png\|left\|200px]]

	{{STRUCTURE_3zth\| PDB=3zth \| SCENE= }}		{{STRUCTURE_3zth\| PDB=3zth \| SCENE= }}
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	===Crystal structure of Stu0660 of Streptococcus thermophilus===		===Crystal structure of Stu0660 of Streptococcus thermophilus===

		+	{{ABSTRACT_PUBMED_22753072}}

	==About this Structure==		==About this Structure==
	[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].		[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022753072</ref><references group="xtra"/>
	[[Category: Streptococcus thermophilus]]		[[Category: Streptococcus thermophilus]]
	[[Category: Lee, K H.]]		[[Category: Lee, K H.]]

OCA at 08:21, 11 July 2012

OCA — Wed, 11 Jul 2012 08:21:35 GMT

←Older revision		Revision as of 08:21, 11 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zth.jpg\|left\|200px]]

-	~~The entry~~ 3zth ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3zth\| PDB=3zth \| SCENE= }}

-	~~Authors: Lee, K.-H., Robinson, H., Oh, B.-H.~~	+	===Crystal structure of Stu0660 of Streptococcus thermophilus===

-	~~Description: Crystal~~ structure ~~of Stu0660 of~~ Streptococcus thermophilus	+
		+	==About this Structure==
		+	[[3zth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTH OCA].
		+	[[Category: Streptococcus thermophilus]]
		+	[[Category: Lee, K H.]]
		+	[[Category: Oh, B H.]]
		+	[[Category: Robinson, H.]]
		+	[[Category: Ca]]
		+	[[Category: Crispr]]
		+	[[Category: Dna binding]]

OCA at 05:40, 10 August 2011

OCA — Wed, 10 Aug 2011 05:40:33 GMT

←Older revision		Revision as of 05:40, 10 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zth is ON HOLD	+	The entry 3zth is ON HOLD until Paper Publication

	Authors: Lee, K.-H., Robinson, H., Oh, B.-H.		Authors: Lee, K.-H., Robinson, H., Oh, B.-H.

	Description: Crystal structure of Stu0660 of Streptococcus thermophilus		Description: Crystal structure of Stu0660 of Streptococcus thermophilus

OCA at 06:21, 20 July 2011

OCA — Wed, 20 Jul 2011 06:21:28 GMT

←Older revision		Revision as of 06:21, 20 July 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zth is ON HOLD ~~until sometime in the future~~	+	The entry 3zth is ON HOLD

	Authors: Lee, K.-H., Robinson, H., Oh, B.-H.		Authors: Lee, K.-H., Robinson, H., Oh, B.-H.

	Description: Crystal structure of Stu0660 of Streptococcus thermophilus		Description: Crystal structure of Stu0660 of Streptococcus thermophilus

OCA: Protected "3zth" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2011 06:58:06 GMT

Protected "3zth" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:58, 13 July 2011

OCA: New page: '''Unreleased structure''' The entry 3zth is ON HOLD until sometime in the future Authors: Lee, K.-H., Robinson, H., Oh, B.-H. Description: Crystal structure of Stu0660 of Streptococcu...

OCA — Wed, 13 Jul 2011 06:58:05 GMT

New page: '''Unreleased structure''' The entry 3zth is ON HOLD until sometime in the future Authors: Lee, K.-H., Robinson, H., Oh, B.-H. Description: Crystal structure of Stu0660 of Streptococcu...

New page

'''Unreleased structure'''

The entry 3zth is ON HOLD until sometime in the future

Authors: Lee, K.-H., Robinson, H., Oh, B.-H.

Description: Crystal structure of Stu0660 of Streptococcus thermophilus