3zur - Revision history

OCA at 11:13, 20 December 2023

OCA — Wed, 20 Dec 2023 11:13:36 GMT

←Older revision		Revision as of 11:13, 20 December 2023
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	<StructureSection load='3zur' size='340' side='right'caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3zur' size='340' side='right'caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.71Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [https://pdbe.org/3zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [https://www.ebi.ac.uk/pdbsum/3zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zur ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [https://pdbe.org/3zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [https://www.ebi.ac.uk/pdbsum/3zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zur ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~BXA1_CLOBO BXA1_CLOBO]~~] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.	+	[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.[https://www.uniprot.org/uniprot/SNP25_HUMAN SNP25_HUMAN] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bontoxilysin~~]]	+	[[Category: Clostridium botulinum]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acharya~~, K R~~]]	+	[[Category: Acharya KR]]
-	[[Category: Chaddock~~, J A~~]]	+	[[Category: Chaddock JA]]
-	[[Category: Masuyer, G]]	+	[[Category: Masuyer G]]
-	[[Category: Stancombe, P]]	+	[[Category: Stancombe P]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-signaling protein complex]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Snare~~]]	+

OCA at 07:19, 18 August 2022

OCA — Thu, 18 Aug 2022 07:19:37 GMT

←Older revision		Revision as of 07:19, 18 August 2022
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	==Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A==		==Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A==
-	<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>	+	<StructureSection load='3zur' size='340' side='right'caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [~~http~~://pdbe.org/3zur PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zur PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zur ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [https://pdbe.org/3zur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [https://www.ebi.ac.uk/pdbsum/3zur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zur ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.	+	[[https://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
	[[Category: Chaddock, J A]]		[[Category: Chaddock, J A]]

OCA at 16:35, 27 February 2019

OCA — Wed, 27 Feb 2019 16:35:43 GMT

←Older revision		Revision as of 16:35, 27 February 2019
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	<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
		+	[[Category: Human]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
	[[Category: Chaddock, J A]]		[[Category: Chaddock, J A]]

OCA at 13:31, 15 March 2017

OCA — Wed, 15 Mar 2017 13:31:45 GMT

←Older revision		Revision as of 13:31, 15 March 2017
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	<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]

OCA at 16:01, 4 August 2016

OCA — Thu, 04 Aug 2016 16:01:22 GMT

←Older revision		Revision as of 16:01, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A==		==Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A==
	<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [http://www.ebi.ac.uk/pdbsum/3zur PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [http://pdbe.org/3zur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [http://www.ebi.ac.uk/pdbsum/3zur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zur ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zur" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:50, 25 December 2014

OCA — Thu, 25 Dec 2014 10:50:07 GMT

←Older revision		Revision as of 10:50, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [http://www.ebi.ac.uk/pdbsum/3zur PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [http://www.ebi.ac.uk/pdbsum/3zur PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BXA1_CLOBO BXA1_CLOBO]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: Acharya, K R.]]	+	[[Category: Acharya, K R]]
-	[[Category: Chaddock, J A.]]	+	[[Category: Chaddock, J A]]
-	[[Category: Masuyer, G.]]	+	[[Category: Masuyer, G]]
-	[[Category: Stancombe, P.]]	+	[[Category: Stancombe, P]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase-signaling protein complex]]		[[Category: Hydrolase-signaling protein complex]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
	[[Category: Snare]]		[[Category: Snare]]

OCA at 07:38, 5 June 2014

OCA — Thu, 05 Jun 2014 07:38:31 GMT

←Older revision		Revision as of 07:38, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3zur.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A==
		+	<StructureSection load='3zur' size='340' side='right' caption='[[3zur]], [[Resolution\|resolution]] 2.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zur]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kil\|1kil]], [[1xtg\|1xtg]], [[1uee\|1uee]], [[2w2d\|2w2d]], [[2vu9\|2vu9]], [[3bta\|3bta]], [[1xtf\|1xtf]], [[2vua\|2vua]], [[3zuq\|3zuq]], [[3zus\|3zus]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zur RCSB], [http://www.ebi.ac.uk/pdbsum/3zur PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Targeted secretion inhibitors (TSIs) are a new class of engineered biopharmaceutical molecules derived from the botulinum neurotoxins (BoNTs). They consist of the metalloprotease light chain (LC) and translocation domain (Hn) of BoNT; they thus lack the native toxicity towards motor neurons but are able to target soluble N-ethylmaleimide-sensitive fusion protein attachment receptor (SNARE) proteins. These functional fragment (LHn) derivatives are expressed as single-chain proteins and require post-translational activation into di-chain molecules for function. A range of BoNT derivatives have been produced to demonstrate the successful use of engineered SNARE substrate peptides at the LC-Hn interface that gives these molecules self-activating capabilities. Alternatively, recognition sites for specific exoproteases can be engineered to allow controlled activation. Here, the crystal structures of three LHn derivatives are reported between 2.7 and 3.0 A resolution. Two of these molecules are derivatives of serotype A that contain a SNARE peptide. Additionally, a third structure corresponds to LHn serotype B that includes peptide linkers at the exoprotease activation site. In all three cases the added engineered segments could not be modelled owing to disorder. However, these structures highlight the strong interactions holding the LHn fold together despite the inclusion of significant polypeptide sequences at the LC-Hn interface.

-	~~<!--~~	+	Structures of engineered Clostridium botulinum neurotoxin derivatives.,Masuyer G, Stancombe P, Chaddock JA, Acharya KR Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1466-72., Epub 2011 Nov 25. PMID:22139146<ref>PMID:22139146</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3zur"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3zur\| PDB=3zur \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22139146}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22139146 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+	[[Category: Bacillus botulinus van ermengem 1896]]
-	~~{{ABSTRACT_PUBMED_22139146}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3zur]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Clostridium_botulinum,_homo_sapiens Clostridium botulinum, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022139146<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: Clostridium botulinum, homo sapiens]]
	[[Category: Acharya, K R.]]		[[Category: Acharya, K R.]]
	[[Category: Chaddock, J A.]]		[[Category: Chaddock, J A.]]

OCA at 06:56, 28 March 2012

OCA — Wed, 28 Mar 2012 06:56:14 GMT

←Older revision		Revision as of 06:56, 28 March 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zur.png\|left\|200px]]

-	The ~~entry 3zur~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zur", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zur\| PDB=3zur \| SCENE= }}

-	~~Authors: Masuyer~~, ~~G., Stancombe, P., Chaddock, J.~~A~~., Acharya, K.R.~~	+	===Crystal structure of an engineered botulinum neurotoxin type A- SNARE23 derivative, LC0-A-SNAP25-Hn-A===

-	~~Description~~: ~~Crystal~~ structure ~~of an engineered~~ botulinum ~~neurotoxin type A-SNARE23 derivative~~, ~~LC0~~-A-~~SNAP25-Hn-A~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22139146}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22139146 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22139146}}
		+
		+	==About this Structure==
		+	[[3zur]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum,_homo_sapiens Clostridium botulinum, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022139146</ref><references group="xtra"/>
		+	[[Category: Bontoxilysin]]
		+	[[Category: Clostridium botulinum, homo sapiens]]
		+	[[Category: Acharya, K R.]]
		+	[[Category: Chaddock, J A.]]
		+	[[Category: Masuyer, G.]]
		+	[[Category: Stancombe, P.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-signaling protein complex]]
		+	[[Category: Protein engineering]]
		+	[[Category: Snare]]

OCA at 08:29, 3 August 2011

OCA — Wed, 03 Aug 2011 08:29:50 GMT

←Older revision		Revision as of 08:29, 3 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zur is ON HOLD	+	The entry 3zur is ON HOLD until Paper Publication

	Authors: Masuyer, G., Stancombe, P., Chaddock, J.A., Acharya, K.R.		Authors: Masuyer, G., Stancombe, P., Chaddock, J.A., Acharya, K.R.

	Description: Crystal structure of an engineered botulinum neurotoxin type A-SNARE23 derivative, LC0-A-SNAP25-Hn-A		Description: Crystal structure of an engineered botulinum neurotoxin type A-SNARE23 derivative, LC0-A-SNAP25-Hn-A

OCA: Protected "3zur" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2011 05:49:18 GMT

Protected "3zur" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 27 July 2011