3zvc - Revision history

OCA at 11:13, 20 December 2023

OCA — Wed, 20 Dec 2023 11:13:59 GMT

←Older revision		Revision as of 11:13, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zvc' size='340' side='right'caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3zvc' size='340' side='right'caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ev-d68 Ev-d68~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_D68 Enterovirus D68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [https://pdbe.org/3zvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [https://www.ebi.ac.uk/pdbsum/3zvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [https://pdbe.org/3zvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [https://www.ebi.ac.uk/pdbsum/3zvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~A1E4A3_9ENTO A1E4A3_9ENTO~~]] ~~Protein 2C associates~~ with and induces ~~structural rearrangements~~ of ~~intracellular membranes~~. ~~It displays~~ RNA~~-binding~~, ~~nucleotide~~ binding and ~~NTPase activities~~ (By similarity).[~~SAAS~~:~~SAAS000199_004_016611~~] ~~Protein 3C is a cysteine~~ protease that ~~generates mature~~ viral proteins ~~from~~ the ~~precursor~~ polyprotein. ~~In addition~~ to ~~its proteolytic activity~~, ~~it binds~~ to ~~viral RNA~~, and ~~thus influences~~ viral genome replication. RNA and ~~substrate bind co~~-~~operatively~~ to the ~~protease~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_042266~~] RNA-directed RNA polymerase 3D-~~POL replicates~~ genomic and ~~antigenomic~~ RNA ~~by recognizing replications specific signals~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_010047~~]	+	[https://www.uniprot.org/uniprot/A1E4A3_HED68 A1E4A3_HED68] Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU (By similarity). The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome (By similarity). Following genome release from the infecting virion in the cytoplasm, the VPg-RNA linkage is probably removed by host TDP2 (By similarity). During the late stage of the replication cycle, host TDP2 is excluded from sites of viral RNA synthesis and encapsidation, allowing for the generation of progeny virions.[ARBA:ARBA00024846] Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step.[RuleBase:RU364118] Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks.[RuleBase:RU364118] Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm.[RuleBase:RU364118] Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation (By similarity). Allows the capsid to remain inactive before the maturation step.[ARBA:ARBA00025202] Protease 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins.[RuleBase:RU364118] Protease 3C: Major viral protease that mediates proteolytic processing of the polyprotein. Cleaves host EIF5B, contributing to host translation shutoff. Cleaves also host PABPC1, contributing to host translation shutoff.[RuleBase:RU364118] Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication.[RuleBase:RU364118] Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3.[RuleBase:RU364118] Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the disassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface.[RuleBase:RU364118] Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity.[RuleBase:RU364118] Protein 3CD: Involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. Protein 3CD binds to the 5'UTR of the viral genome.[RuleBase:RU364118] RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated.[RuleBase:RU364118] Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome.[RuleBase:RU364118]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ev-d68~~]]	+	[[Category: Enterovirus D68]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Picornain 3C]]~~	+	[[Category: Hilgenfeld R]]
-	[[Category: Hilgenfeld, R]]	+	[[Category: Mesters JR]]
-	[[Category: Mesters~~, J R~~]]	+	[[Category: Perbandt M]]
-	[[Category: Perbandt, M]]	+	[[Category: Tan J]]
-	[[Category: Tan, J]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Michael inhibitor~~]]	+

OCA at 07:20, 18 August 2022

OCA — Thu, 18 Aug 2022 07:20:48 GMT

←Older revision		Revision as of 07:20, 18 August 2022
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	<StructureSection load='3zvc' size='340' side='right'caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3zvc' size='340' side='right'caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ev-d68 Ev-d68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ev-d68 Ev-d68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [~~http~~://pdbe.org/3zvc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zvc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [https://pdbe.org/3zvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [https://www.ebi.ac.uk/pdbsum/3zvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/A1E4A3_9ENTO A1E4A3_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]	+	[[https://www.uniprot.org/uniprot/A1E4A3_9ENTO A1E4A3_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Virus protease\|Virus protease]]	+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:31, 10 May 2019

OCA — Fri, 10 May 2019 12:31:49 GMT

←Older revision		Revision as of 12:31, 10 May 2019
Line 1:		Line 1:

	==3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82==		==3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82==
-	<StructureSection load='3zvc' size='340' side='right' caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3zvc' size='340' side='right'caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-d68 Ev-d68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-d68 Ev-d68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[3C protease\|3C protease]]	+	*[[Virus protease\|Virus protease]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Ev-d68]]		[[Category: Ev-d68]]
		+	[[Category: Large Structures]]
	[[Category: Picornain 3C]]		[[Category: Picornain 3C]]
	[[Category: Hilgenfeld, R]]		[[Category: Hilgenfeld, R]]

OCA at 10:55, 5 August 2016

OCA — Fri, 05 Aug 2016 10:55:31 GMT

←Older revision		Revision as of 10:55, 5 August 2016
Line 1:		Line 1:
		+
	==3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82==		==3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82==
	<StructureSection load='3zvc' size='340' side='right' caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3zvc' size='340' side='right' caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_enterovirus_68 Human enterovirus 68~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-d68 Ev-d68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [http://www.ebi.ac.uk/pdbsum/3zvc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [http://pdbe.org/3zvc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [http://www.ebi.ac.uk/pdbsum/3zvc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zvc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3C protease\|3C protease]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human enterovirus 68~~]]	+	[[Category: Ev-d68]]
	[[Category: Picornain 3C]]		[[Category: Picornain 3C]]
	[[Category: Hilgenfeld, R]]		[[Category: Hilgenfeld, R]]

OCA at 18:12, 25 December 2014

OCA — Thu, 25 Dec 2014 18:12:52 GMT

←Older revision		Revision as of 18:12, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [http://www.ebi.ac.uk/pdbsum/3zvc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [http://www.ebi.ac.uk/pdbsum/3zvc PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A1E4A3_9ENTO A1E4A3_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:13, 21 December 2014

OCA — Sun, 21 Dec 2014 09:13:00 GMT

←Older revision		Revision as of 09:13, 21 December 2014
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-	~~{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}~~	+	==3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82==
-	===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===	+	<StructureSection load='3zvc' size='340' side='right' caption='[[3zvc]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	{~~{ABSTRACT_PUBMED_23388726}~~}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_68 Human enterovirus 68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G82:ETHYL+(5S,8S,11R)-8-BENZYL-5-(3-TERT-BUTOXY-3-OXOPROPYL)-3,6,9-TRIOXO-11-{[(3S)-2-OXOPYRROLIDIN-3-YL]METHYL}-1-PHENYL-2-OXA-4,7,10-TRIAZATETRADECAN-14-OATE'>G82</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zv8\|3zv8]], [[3zva\|3zva]], [[3zve\|3zve]], [[3zvf\|3zvf]], [[3zvb\|3zvb]], [[3zvg\|3zvg]], [[3zv9\|3zv9]], [[3zvd\|3zvd]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zvc RCSB], [http://www.ebi.ac.uk/pdbsum/3zvc PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the cleavage specificity and the crystal structure of the 3C protease of enterovirus 68 (EV68 3C(pro)). The protease exhibits a typical chymotrypsin fold with a Cys...His...Glu catalytic triad; its three-dimensional structure is closely related to that of the 3C(pro) of rhinovirus 2 as well as to that of poliovirus. The phylogenetic position of the EV68 3C(pro) between the corresponding enzymes of rhinoviruses on the one hand and enteroviruses on the other prompted us to use the crystal structure for the design of irreversible inhibitors, with the goal of discovering broad-spectrum antiviral compounds. We synthesized a series of peptidic alpha,beta-unsaturated ethyl esters of increasing length and for each inhibitor candidate, we determined a crystal structure of its complex with the EV68 3C(pro), which served as the basis for the next design round. To exhibit inhibitory activity, compounds must span at least P3 to P1' ; the most potent inhibitors comprise P4 to P1' . Inhibitory activities were found against the purified 3C protease of EV68 as well as with replicons for poliovirus and EV71 (EC(50) = 0.5 muM for the best compound). Antiviral activities were determined using cell cultures infected with EV71, poliovirus, echovirus 11, and various rhinovirus serotypes. The most potent inhibitor, SG85, exhibited activity with EC(50) values of approximately 180 nM against EV71 and approximately 60 nM against human rhinovirus 14 in a live virus-cell-based assay. Even the shorter SG75, spanning only P3 to P1' , displayed significant activity (EC(50) = 2 to 5 muM) against various rhinoviruses.

-	~~==Function==~~	+	3C protease of enterovirus 68: Structure-based design of Michael acceptor inhibitors and their broad-spectrum antiviral effects against picornaviruses.,Tan J, George S, Kusov Y, Perbandt M, Anemuller S, Mesters JR, Norder H, Coutard B, Lacroix C, Leyssen P, Neyts J, Hilgenfeld R J Virol. 2013 Feb 6. PMID:23388726<ref>PMID:23388726</ref>
-	~~[[http~~:~~//www.uniprot.org/uniprot/A1E4A3_9ENTO A1E4A3_9ENTO]] Protein 2C associates with~~ and ~~induces structural rearrangements of intracellular membranes. It displays RNA~~-~~binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein~~. ~~In addition to its proteolytic activity~~, ~~it binds to viral RNA~~, ~~and thus influences viral genome replication~~. ~~RNA and substrate bind co-operatively to the protease (By similarity)~~.~~[SAAS~~:~~SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS~~:~~SAAS000199_004_010047]~~	+

-	==~~About this Structure~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3zvc]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Human_enterovirus_68 Human enterovirus 68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA].~~	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Human enterovirus 68]]		[[Category: Human enterovirus 68]]
	[[Category: Picornain 3C]]		[[Category: Picornain 3C]]
-	[[Category: Hilgenfeld, R.]]	+	[[Category: Hilgenfeld, R]]
-	[[Category: Mesters, J R.]]	+	[[Category: Mesters, J R]]
-	[[Category: Perbandt, M.]]	+	[[Category: Perbandt, M]]
-	[[Category: Tan, J.]]	+	[[Category: Tan, J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Michael inhibitor]]		[[Category: Michael inhibitor]]

OCA at 21:43, 10 April 2013

OCA — Wed, 10 Apr 2013 21:43:23 GMT

←Older revision		Revision as of 21:43, 10 April 2013
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-	[[Image:3zvc.png\|left\|200px]]
-
	{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}		{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}
-
	===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===		===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===
		+	{{ABSTRACT_PUBMED_23388726}}

-	~~{{ABSTRACT_PUBMED_23388726}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/A1E4A3_9ENTO A1E4A3_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

	==About this Structure==		==About this Structure==

OCA at 13:38, 27 February 2013

OCA — Wed, 27 Feb 2013 13:38:01 GMT

←Older revision		Revision as of 13:38, 27 February 2013
Line 1:		Line 1:
-	[[Image:3zvc.~~jpg~~\|left\|200px]]	+	[[Image:3zvc.png\|left\|200px]]

	{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}		{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}
Line 5:		Line 5:
	===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===		===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===

		+	{{ABSTRACT_PUBMED_23388726}}

	==About this Structure==		==About this Structure==
-	[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_enterovirus~~ Human enterovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA].	+	[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_68 Human enterovirus 68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA].
-	[[Category: Human enterovirus]]	+	[[Category: Human enterovirus 68]]
	[[Category: Picornain 3C]]		[[Category: Picornain 3C]]
	[[Category: Hilgenfeld, R.]]		[[Category: Hilgenfeld, R.]]

OCA at 05:30, 8 August 2012

OCA — Wed, 08 Aug 2012 05:30:37 GMT

←Older revision		Revision as of 05:30, 8 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zvc.jpg\|left\|200px]]

-	~~The entry~~ 3zvc ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3zvc\| PDB=3zvc \| SCENE= }}

-	~~Authors: Tan, J., Perbandt, M., Mesters, J.R., Hilgenfeld, R.~~	+	===3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82===

-	~~Description~~: 3C ~~protease of Enterovirus 68 complexed with~~ Michael ~~receptor~~ inhibitor 82	+
		+	==About this Structure==
		+	[[3zvc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus Human enterovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVC OCA].
		+	[[Category: Human enterovirus]]
		+	[[Category: Picornain 3C]]
		+	[[Category: Hilgenfeld, R.]]
		+	[[Category: Mesters, J R.]]
		+	[[Category: Perbandt, M.]]
		+	[[Category: Tan, J.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Michael inhibitor]]

OCA at 08:17, 29 February 2012

OCA — Wed, 29 Feb 2012 08:17:48 GMT

←Older revision		Revision as of 08:17, 29 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zvc is ON HOLD	+	The entry 3zvc is ON HOLD until Paper Publication

	Authors: Tan, J., Perbandt, M., Mesters, J.R., Hilgenfeld, R.		Authors: Tan, J., Perbandt, M., Mesters, J.R., Hilgenfeld, R.

	Description: 3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82		Description: 3C protease of Enterovirus 68 complexed with Michael receptor inhibitor 82