3zxl - Revision history

OCA at 11:15, 20 December 2023

OCA — Wed, 20 Dec 2023 11:15:32 GMT

←Older revision		Revision as of 11:15, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zxl' size='340' side='right'caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='3zxl' size='340' side='right'caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_16454 Atcc 16454~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=B3P:2~~-[~~3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO~~]~~-2-HYDROXYMETHYL-PROPANE-~~1~~,3-DIOL'>B3P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.871Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3zxj\|3zxj]], [[3zxk\|3zxk]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Non~~-~~reducing_end_alpha~~-L-~~arabinofuranosidase Non~~-~~reducing end alpha~~-L-~~arabinofuranosidase~~]~~, with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.~~2.1~~.55~~ 3~~.2.1.55]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [https://pdbe.org/3zxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [https://www.ebi.ac.uk/pdbsum/3zxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zxl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [https://pdbe.org/3zxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [https://www.ebi.ac.uk/pdbsum/3zxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zxl ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 16454~~]]	+	[[Category: Humicola insolens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Non-reducing end alpha-L-arabinofuranosidase]]~~	+	[[Category: Gilbert HJ]]
-	[[Category: Gilbert~~, H J~~]]	+	[[Category: Jackson A]]
-	[[Category: Jackson, A]]	+	[[Category: Krogh KBRM]]
-	[[Category: Krogh~~, K B.R M~~]]	+	[[Category: Lewis RJ]]
-	[[Category: Lewis~~, R J~~]]	+	[[Category: Marles-Wright J]]
-	[[Category: Marles-Wright, J]]	+	[[Category: McKee LS]]
-	[[Category: McKee~~, L S~~]]	+	[[Category: Pena MJ]]
-	[[Category: Pena~~, M J~~]]	+	[[Category: Rogowski A]]
-	[[Category: Rogowski, A]]	+	[[Category: Skjot M]]
-	[[Category: Skjot, M]]	+	[[Category: Vikso-Nielsen A]]
-	[[Category: Vikso-Nielsen, A]]	+	[[Category: York WS]]
-	[[Category: York~~, W S]]~~	+
-	~~[[Category: Arabinosidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Xylosidase~~]]	+

OCA at 07:25, 18 August 2022

OCA — Thu, 18 Aug 2022 07:25:51 GMT

←Older revision		Revision as of 07:25, 18 August 2022
Line 3:		Line 3:
	<StructureSection load='3zxl' size='340' side='right'caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='3zxl' size='340' side='right'caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zxj\|3zxj]], [[3zxk\|3zxk]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Non-reducing_end_alpha-L-arabinofuranosidase Non-reducing end alpha-L-arabinofuranosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-reducing_end_alpha-L-arabinofuranosidase Non-reducing end alpha-L-arabinofuranosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [~~http~~://pdbe.org/3zxl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zxl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zxl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [https://pdbe.org/3zxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [https://www.ebi.ac.uk/pdbsum/3zxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zxl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:01, 29 January 2020

OCA — Wed, 29 Jan 2020 17:01:54 GMT

←Older revision		Revision as of 17:01, 29 January 2020
Line 1:		Line 1:

	==Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity==		==Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity==
-	<StructureSection load='3zxl' size='340' side='right' caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>	+	<StructureSection load='3zxl' size='340' side='right'caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 16454]]		[[Category: Atcc 16454]]
		+	[[Category: Large Structures]]
	[[Category: Non-reducing end alpha-L-arabinofuranosidase]]		[[Category: Non-reducing end alpha-L-arabinofuranosidase]]
	[[Category: Gilbert, H J]]		[[Category: Gilbert, H J]]

OCA at 06:35, 4 April 2018

OCA — Wed, 04 Apr 2018 06:35:17 GMT

←Older revision		Revision as of 06:35, 4 April 2018
Line 29:		Line 29:
	[[Category: Lewis, R J]]		[[Category: Lewis, R J]]
	[[Category: Marles-Wright, J]]		[[Category: Marles-Wright, J]]
-	[[Category: ~~Mckee~~, L S]]	+	[[Category: McKee, L S]]
	[[Category: Pena, M J]]		[[Category: Pena, M J]]
	[[Category: Rogowski, A]]		[[Category: Rogowski, A]]

OCA at 20:57, 11 August 2016

OCA — Thu, 11 Aug 2016 20:57:03 GMT

←Older revision		Revision as of 20:57, 11 August 2016
Line 1:		Line 1:
		+
	==Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity==		==Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity==
	<StructureSection load='3zxl' size='340' side='right' caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='3zxl' size='340' side='right' caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Humicola_insolens Humicola insolens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Alpha~~-N-arabinofuranosidase ~~Alpha~~-N-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-reducing_end_alpha-L-arabinofuranosidase Non-reducing end alpha-L-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [http://www.ebi.ac.uk/pdbsum/3zxl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [http://pdbe.org/3zxl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [http://www.ebi.ac.uk/pdbsum/3zxl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zxl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zxl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alpha-N-arabinofuranosidase~~]]	+	[[Category: Atcc 16454]]
-	[[Category: ~~Humicola insolens~~]]	+	[[Category: Non-reducing end alpha-L-arabinofuranosidase]]
	[[Category: Gilbert, H J]]		[[Category: Gilbert, H J]]
	[[Category: Jackson, A]]		[[Category: Jackson, A]]

OCA at 10:42, 4 January 2015

OCA — Sun, 04 Jan 2015 10:42:33 GMT

←Older revision		Revision as of 10:42, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-N-arabinofuranosidase Alpha-N-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-N-arabinofuranosidase Alpha-N-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [http://www.ebi.ac.uk/pdbsum/3zxl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [http://www.ebi.ac.uk/pdbsum/3zxl PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	[[Category: Alpha-N-arabinofuranosidase]]		[[Category: Alpha-N-arabinofuranosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]
-	[[Category: Gilbert, H J.]]	+	[[Category: Gilbert, H J]]
-	[[Category: Jackson, A.]]	+	[[Category: Jackson, A]]
-	[[Category: Krogh, K B.R M.]]	+	[[Category: Krogh, K B.R M]]
-	[[Category: Lewis, R J.]]	+	[[Category: Lewis, R J]]
-	[[Category: Marles-Wright, J.]]	+	[[Category: Marles-Wright, J]]
-	[[Category: Mckee, L S.]]	+	[[Category: Mckee, L S]]
-	[[Category: Pena, M J.]]	+	[[Category: Pena, M J]]
-	[[Category: Rogowski, A.]]	+	[[Category: Rogowski, A]]
-	[[Category: Skjot, M.]]	+	[[Category: Skjot, M]]
-	[[Category: Vikso-Nielsen, A.]]	+	[[Category: Vikso-Nielsen, A]]
-	[[Category: York, W S.]]	+	[[Category: York, W S]]
	[[Category: Arabinosidase]]		[[Category: Arabinosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Xylosidase]]		[[Category: Xylosidase]]

OCA at 07:50, 5 June 2014

OCA — Thu, 05 Jun 2014 07:50:56 GMT

←Older revision		Revision as of 07:50, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3zxl.~~png~~\|~~left~~\|~~200px~~]]	+	==Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity==
		+	<StructureSection load='3zxl' size='340' side='right' caption='[[3zxl]], [[Resolution\|resolution]] 1.87Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZXL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zxj\|3zxj]], [[3zxk\|3zxk]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-N-arabinofuranosidase Alpha-N-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zxl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zxl RCSB], [http://www.ebi.ac.uk/pdbsum/3zxl PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. The major polysaccharides of the plant cell wall are cellulose and xylan, a highly decorated beta-1,4-xylopyranose polymer. Glycoside hydrolases displaying multiple catalytic functions may simplify the enzymes required to degrade plant cell walls, increasing the industrial potential of these composite structures. Here we test the hypothesis that glycoside hydrolase family 43 (GH43) provides a suitable scaffold for introducing additional catalytic functions into enzymes that target complex structures in the plant cell wall. We report the crystal structure of Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase that hydrolyses O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. HiAXHd3 displays an N-terminal five-bladed beta-propeller domain and a C-terminal beta-sandwich domain. The interface between the domains comprises a xylan binding cleft that houses the active site pocket. Substrate specificity is conferred by a shallow arabinose binding pocket adjacent to the deep active site pocket, and through the orientation of the xylan backbone. Modification of the rim of the active site introduces endo-xylanase activity, whereas the resultant enzyme variant, Y166A, retains arabinofuranosidase activity. These data show that the active site of HiAXHd3 is tuned to hydrolyse arabinofuranosyl or xylosyl linkages, and it is the topology of the distal regions of the substrate binding surface that confers specificity. This report demonstrates that GH43 provides a platform for generating bespoke multifunctional enzymes that target industrially significant complex substrates, exemplified by the plant cell wall.

-	<!--	+	Introducing endo-xylanase activity into an exo-acting arabinofuranosidase that targets side chains.,McKee LS, Pena MJ, Rogowski A, Jackson A, Lewis RJ, York WS, Krogh KB, Vikso-Nielsen A, Skjot M, Gilbert HJ, Marles-Wright J Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6537-42. Epub 2012 Apr 6. PMID:22492980<ref>PMID:22492980</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3zxl"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3zxl\| PDB=3zxl \| SCENE= }}~~	+

-	~~===Engineering the active site~~ of ~~a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22492980}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22492980 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22492980}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3zxl]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022492980<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Alpha-N-arabinofuranosidase]]		[[Category: Alpha-N-arabinofuranosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]

OCA at 18:13, 2 May 2012

OCA — Wed, 02 May 2012 18:13:32 GMT

←Older revision		Revision as of 18:13, 2 May 2012
Line 1:		Line 1:
-	[[Image:3zxl.~~jpg~~\|left\|200px]]	+	[[Image:3zxl.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity===		===Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22492980}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22492980 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22492980}}

	==About this Structure==		==About this Structure==
	[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA].		[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022492980</ref><references group="xtra"/>
	[[Category: Alpha-N-arabinofuranosidase]]		[[Category: Alpha-N-arabinofuranosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]

OCA at 07:59, 18 April 2012

OCA — Wed, 18 Apr 2012 07:59:04 GMT

←Older revision		Revision as of 07:59, 18 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zxl.jpg\|left\|200px]]

-	The ~~entry 3zxl~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zxl", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zxl\| PDB=3zxl \| SCENE= }}

-	~~Authors: McKee, L.S., Pena, M.J., Rogowski, A., Jackson, A., Lewis, R.J., York, W.S., Krogh, K.B.R.M., Vikso~~-~~Nielsen, A., Skjot, M., Gilbert, H.J., Marles~~-~~Wright, J.~~	+	===Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity===

-	~~Description: Engineering the active site of~~ a ~~GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo~~-~~xylanase and exo~~-arabinofuranosidase ~~activity~~	+
		+	==About this Structure==
		+	[[3zxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZXL OCA].
		+	[[Category: Alpha-N-arabinofuranosidase]]
		+	[[Category: Humicola insolens]]
		+	[[Category: Gilbert, H J.]]
		+	[[Category: Jackson, A.]]
		+	[[Category: Krogh, K B.R M.]]
		+	[[Category: Lewis, R J.]]
		+	[[Category: Marles-Wright, J.]]
		+	[[Category: Mckee, L S.]]
		+	[[Category: Pena, M J.]]
		+	[[Category: Rogowski, A.]]
		+	[[Category: Skjot, M.]]
		+	[[Category: Vikso-Nielsen, A.]]
		+	[[Category: York, W S.]]
		+	[[Category: Arabinosidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Xylosidase]]

OCA at 05:53, 7 September 2011

OCA — Wed, 07 Sep 2011 05:53:54 GMT

←Older revision		Revision as of 05:53, 7 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zxl is ON HOLD	+	The entry 3zxl is ON HOLD until Paper Publication

	Authors: McKee, L.S., Pena, M.J., Rogowski, A., Jackson, A., Lewis, R.J., York, W.S., Krogh, K.B.R.M., Vikso-Nielsen, A., Skjot, M., Gilbert, H.J., Marles-Wright, J.		Authors: McKee, L.S., Pena, M.J., Rogowski, A., Jackson, A., Lewis, R.J., York, W.S., Krogh, K.B.R.M., Vikso-Nielsen, A., Skjot, M., Gilbert, H.J., Marles-Wright, J.

	Description: Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo-xylanase and exo-arabinofuranosidase activity		Description: Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo-xylanase and exo-arabinofuranosidase activity