3zyz - Revision history

OCA at 07:29, 18 August 2022

OCA — Thu, 18 Aug 2022 07:29:18 GMT

←Older revision		Revision as of 07:29, 18 August 2022
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	==Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.==		==Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.==
-	<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3zyz' size='340' side='right'caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zyz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZYZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zyz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZYZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zz1\|3zz1]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyz OCA], [~~http~~://pdbe.org/3zyz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zyz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zyz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zyz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyz OCA], [https://pdbe.org/3zyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zyz RCSB], [https://www.ebi.ac.uk/pdbsum/3zyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zyz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Beta-glucosidase\|Beta-glucosidase]]	+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
	[[Category: Hypocrea jecorina]]		[[Category: Hypocrea jecorina]]
		+	[[Category: Large Structures]]
	[[Category: Gudmundsson, M]]		[[Category: Gudmundsson, M]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]

OCA at 16:44, 27 February 2019

OCA — Wed, 27 Feb 2019 16:44:49 GMT

←Older revision		Revision as of 16:44, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zyz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trichoderma_reesei Trichoderma reesei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZYZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zyz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZYZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
-	[[Category: ~~Trichoderma reesei~~]]	+	[[Category: Hypocrea jecorina]]
	[[Category: Gudmundsson, M]]		[[Category: Gudmundsson, M]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]

OCA at 20:58, 11 August 2016

OCA — Thu, 11 Aug 2016 20:58:08 GMT

←Older revision		Revision as of 20:58, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.==		==Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.==
	<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zyz RCSB], [http://www.ebi.ac.uk/pdbsum/3zyz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyz OCA], [http://pdbe.org/3zyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zyz RCSB], [http://www.ebi.ac.uk/pdbsum/3zyz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zyz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zyz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:16, 19 November 2014

OCA — Wed, 19 Nov 2014 09:16:24 GMT

←Older revision		Revision as of 09:16, 19 November 2014
Line 25:		Line 25:
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
	[[Category: Trichoderma reesei]]		[[Category: Trichoderma reesei]]
-	[[Category: Gudmundsson, M.]]	+	[[Category: Gudmundsson, M]]
-	[[Category: Hansson, H.]]	+	[[Category: Hansson, H]]
-	[[Category: Kaper, T.]]	+	[[Category: Kaper, T]]
-	[[Category: Karkehabadi, S.]]	+	[[Category: Karkehabadi, S]]
-	[[Category: Kelemen, B.]]	+	[[Category: Kelemen, B]]
-	[[Category: Larenas, E.]]	+	[[Category: Larenas, E]]
-	[[Category: Mikkelsen, N E.]]	+	[[Category: Mikkelsen, N E]]
-	[[Category: Piens, K.]]	+	[[Category: Piens, K]]
-	[[Category: Sandgren, M.]]	+	[[Category: Sandgren, M]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:17, 22 October 2014

OCA — Wed, 22 Oct 2014 08:17:41 GMT

←Older revision		Revision as of 08:17, 22 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3zyz.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.==
		+	<StructureSection load='3zyz' size='340' side='right' caption='[[3zyz]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zyz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZYZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zz1\|3zz1]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zyz RCSB], [http://www.ebi.ac.uk/pdbsum/3zyz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulase mixtures from H. jecorina are commonly used for the saccharification of cellulose in biotechnical applications. The most abundant beta-glucosidase in the mesophilic fungus Hypocrea jecorina is HjCel3A, which hydrolyzes the beta-linkage between two adjacent molecules in dimers and short oligomers of glucose. It has been shown that enhanced levels of HjCel3A in H. jecorina cellulase mixtures benefit the conversion of cellulose to glucose. Biochemical characterization of HjCel3A shows that the enzyme efficiently hydrolyzes (1,4)- as well as (1,2)-, (1,3)-, and (1,6)- beta-D linked disaccharides. For crystallization studies, HjCel3A was produced in both H. jecorina (HjCel3A) and Pichia pastoris (Pp-HjCel3A). While the thermostabilities of HjCel3A and Pp-HjCel3A are the same, Pp-HjCel3A has a higher degree of N-linked glycosylation. Here, we present X-ray structures of HjCel3A with and without glucose bound in the active site. The structures have a three-domain architecture as previously observed for other glycoside hydrolase family 3 beta-glucosidases. Both production hosts resulted in HjCel3A structures that have N-linked glycosylations at Asn208 and Asn310. In H. jecorina-produced HjCel3A a single N-acetylglucosamine is present at both sites, while in Pp-HjCel3A the P. pastoris-produced HjCel3A enzyme the glycan chains consist of 8 or 4 saccharides, respectively. The glycosylations are involved in intermolecular contacts in the structures derived from either host. Due to the different sizes of the glycosylations, the interactions result in different crystal forms for the two protein forms.

-	~~{{STRUCTURE_3zyz\| PDB=3zyz \| SCENE= }}~~	+	Biochemical Characterization and Crystal Structures of a Fungal Family 3 beta-Glucosidase, Cel3A from Hypocrea jecorina.,Karkehabadi S, Helmich KE, Kaper T, Hansson H, Mikkelsen NE, Gudmundsson M, Piens K, Fujdala M, Banerjee G, Scott-Craig JS, Walton JD, Phillips GN Jr, Sandgren M J Biol Chem. 2014 Aug 27. pii: jbc.M114.587766. PMID:25164811<ref>PMID:25164811</ref>

-	~~===Crystal structure of a glycoside hydrolase family 3 beta-glucosidase~~, ~~Bgl1 from Hypocrea jecorina at 2~~.~~1A resolution~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	==~~About this Structure~~==	+	*[[Beta-glucosidase\|Beta-glucosidase]]
-	[[~~3zyz~~]] ~~is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA].~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
	[[Category: Trichoderma reesei]]		[[Category: Trichoderma reesei]]

OCA at 21:23, 12 December 2012

OCA — Wed, 12 Dec 2012 21:23:14 GMT

←Older revision		Revision as of 21:23, 12 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zyz.jpg\|left\|200px]]

-	~~The entry~~ 3zyz ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3zyz\| PDB=3zyz \| SCENE= }}

-	~~Authors: Sandgren~~, M.~~, Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S~~.	+	===Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.===

-	~~Description: Crystal structure of~~ a ~~glycoside hydrolase family 3 beta~~-glucosidase, ~~Bgl1 from Hypocrea jecorina at 2~~.~~1A resolution~~.	+
		+	==About this Structure==
		+	[[3zyz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYZ OCA].
		+	[[Category: Beta-glucosidase]]
		+	[[Category: Trichoderma reesei]]
		+	[[Category: Gudmundsson, M.]]
		+	[[Category: Hansson, H.]]
		+	[[Category: Kaper, T.]]
		+	[[Category: Karkehabadi, S.]]
		+	[[Category: Kelemen, B.]]
		+	[[Category: Larenas, E.]]
		+	[[Category: Mikkelsen, N E.]]
		+	[[Category: Piens, K.]]
		+	[[Category: Sandgren, M.]]
		+	[[Category: Hydrolase]]

OCA at 08:59, 30 October 2011

OCA — Sun, 30 Oct 2011 08:59:49 GMT

←Older revision		Revision as of 08:59, 30 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zyz is ON HOLD	+	The entry 3zyz is ON HOLD until Paper Publication

	Authors: Sandgren, M., Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S.		Authors: Sandgren, M., Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S.

	Description: Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.		Description: Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.

OCA: Protected "3zyz" [edit=sysop:move=sysop]

OCA — Wed, 07 Sep 2011 05:54:21 GMT

Protected "3zyz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:54, 7 September 2011

OCA: New page: '''Unreleased structure''' The entry 3zyz is ON HOLD Authors: Sandgren, M., Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S...

OCA — Wed, 07 Sep 2011 05:54:20 GMT

New page: '''Unreleased structure''' The entry 3zyz is ON HOLD Authors: Sandgren, M., Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S...

New page

'''Unreleased structure'''

The entry 3zyz is ON HOLD

Authors: Sandgren, M., Kaper, T., Mikkelsen, N.E., Hansson, H., Piens, K., Gudmundsson, M., Larenas, E., Kelemen, B., Karkehabadi, S.

Description: Crystal structure of a glycoside hydrolase family 3 beta-glucosidase, Bgl1 from Hypocrea jecorina at 2.1A resolution.