3zzq - Revision history

OCA at 11:16, 20 December 2023

OCA — Wed, 20 Dec 2023 11:16:52 GMT

←Older revision		Revision as of 11:16, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zzq' size='340' side='right'caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3zzq' size='340' side='right'caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1wap\|1wap]], [[3zzl\|3zzl]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [https://pdbe.org/3zzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [https://www.ebi.ac.uk/pdbsum/3zzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [https://pdbe.org/3zzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [https://www.ebi.ac.uk/pdbsum/3zzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MTRB_BACSU MTRB_BACSU]] Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.<ref>PMID:1551827</ref>	+	[https://www.uniprot.org/uniprot/MTRB_BACSU MTRB_BACSU] Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.<ref>PMID:1551827</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Antson~~, A A~~]]	+	[[Category: Antson AA]]
-	[[Category: Chen, C]]	+	[[Category: Chen C]]
-	[[Category: Dodson~~, G G~~]]	+	[[Category: Dodson GG]]
-	[[Category: Gollnick, P]]	+	[[Category: Gollnick P]]
-	[[Category: Merlino, N]]	+	[[Category: Merlino N]]
-	[[Category: Shevtsov~~, M B~~]]	+	[[Category: Shevtsov MB]]
-	[[Category: Smits, C]]	+	[[Category: Smits C]]
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation~~]]	+

OCA at 07:30, 18 August 2022

OCA — Thu, 18 Aug 2022 07:30:47 GMT

←Older revision		Revision as of 07:30, 18 August 2022
Line 1:		Line 1:

	==Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)==		==Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)==
-	<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3zzq' size='340' side='right'caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wap\|1wap]], [[3zzl\|3zzl]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [~~http~~://pdbe.org/3zzq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zzq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [https://pdbe.org/3zzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [https://www.ebi.ac.uk/pdbsum/3zzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MTRB_BACSU MTRB_BACSU]] Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.<ref>PMID:1551827</ref>	+	[[https://www.uniprot.org/uniprot/MTRB_BACSU MTRB_BACSU]] Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.<ref>PMID:1551827</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Antson, A A]]		[[Category: Antson, A A]]
	[[Category: Chen, C]]		[[Category: Chen, C]]

OCA at 16:19, 27 February 2019

OCA — Wed, 27 Feb 2019 16:19:47 GMT

←Older revision		Revision as of 16:19, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Antson, A A]]		[[Category: Antson, A A]]
	[[Category: Chen, C]]		[[Category: Chen, C]]

OCA at 09:28, 19 October 2016

OCA — Wed, 19 Oct 2016 09:28:32 GMT

←Older revision		Revision as of 09:28, 19 October 2016
Line 1:		Line 1:

-	==~~ENGINEERED~~ 12-~~SUBUNIT BACILLUS SUBTILIS TRP~~ RNA-~~BINDING ATTENUATION PROTEIN~~ (TRAP)==	+	==Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)==
	<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 21:04, 5 August 2016

OCA — Fri, 05 Aug 2016 21:04:48 GMT

←Older revision		Revision as of 21:04, 5 August 2016
Line 1:		Line 1:
		+
	==ENGINEERED 12-SUBUNIT BACILLUS SUBTILIS TRP RNA-BINDING ATTENUATION PROTEIN (TRAP)==		==ENGINEERED 12-SUBUNIT BACILLUS SUBTILIS TRP RNA-BINDING ATTENUATION PROTEIN (TRAP)==
	<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [http://www.ebi.ac.uk/pdbsum/3zzq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [http://pdbe.org/3zzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [http://www.ebi.ac.uk/pdbsum/3zzq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zzq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Antson, A A]]		[[Category: Antson, A A]]
	[[Category: Chen, C]]		[[Category: Chen, C]]

OCA at 21:53, 25 December 2014

OCA — Thu, 25 Dec 2014 21:53:53 GMT

←Older revision		Revision as of 21:53, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [http://www.ebi.ac.uk/pdbsum/3zzq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [http://www.ebi.ac.uk/pdbsum/3zzq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MTRB_BACSU MTRB_BACSU]] Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.<ref>PMID:1551827</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	How to Change the Oligomeric State of a Circular Protein Assembly: Switch from 11-Subunit to 12-Subunit TRAP Suggests a General Mechanism.,Chen CS, Smits C, Dodson GG, Shevtsov MB, Merlino N, Gollnick P, Antson AA PLoS One. 2011;6(10):e25296. Epub 2011 Oct 3. PMID:21984911<ref>PMID:21984911</ref>		How to Change the Oligomeric State of a Circular Protein Assembly: Switch from 11-Subunit to 12-Subunit TRAP Suggests a General Mechanism.,Chen CS, Smits C, Dodson GG, Shevtsov MB, Merlino N, Gollnick P, Antson AA PLoS One. 2011;6(10):e25296. Epub 2011 Oct 3. PMID:21984911<ref>PMID:21984911</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Tryptophan RNA-binding attenuation protein\|Tryptophan RNA-binding attenuation protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Antson, A A.]]	+	[[Category: Antson, A A]]
-	[[Category: Chen, C.]]	+	[[Category: Chen, C]]
-	[[Category: Dodson, G G.]]	+	[[Category: Dodson, G G]]
-	[[Category: Gollnick, P.]]	+	[[Category: Gollnick, P]]
-	[[Category: Merlino, N.]]	+	[[Category: Merlino, N]]
-	[[Category: Shevtsov, M B.]]	+	[[Category: Shevtsov, M B]]
-	[[Category: Smits, C.]]	+	[[Category: Smits, C]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 07:18, 5 June 2014

OCA — Thu, 05 Jun 2014 07:18:22 GMT

←Older revision		Revision as of 07:18, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3zzq.~~png~~\|~~left~~\|~~200px~~]]	+	==ENGINEERED 12-SUBUNIT BACILLUS SUBTILIS TRP RNA-BINDING ATTENUATION PROTEIN (TRAP)==
		+	<StructureSection load='3zzq' size='340' side='right' caption='[[3zzq]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wap\|1wap]], [[3zzl\|3zzl]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzq RCSB], [http://www.ebi.ac.uk/pdbsum/3zzq PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligomeric states has prevented engineering such transitions. In this study we used the bacterial transcription regulator TRAP as a model system to investigate the features that define the oligomeric state of a circular protein and to question how the subunit number could be manipulated. METHODOLOGY/PRINCIPAL FINDINGS: We find that while Bacillus subtilis and Bacillus stearothermophilus TRAP form 11-subunit oligomers, the Bacillus halodurans TRAP exclusively forms 12-subunit assemblies. Significantly, the two states of TRAP are related by a simple rigid body rotation of individual subunits around inter-subunit axes. We tested if such a rotation could be induced by insertion or deletion mutations at the subunit interface. Using wild type 11-subunit TRAP, we demonstrate that removal of five C-terminal residues at the outer side of the inter-subunit axis or extension of an amino acid side chain at the opposite, inner side, increased the subunit number from 11 to 12. Our findings are supported by crystal structures of TRAP oligomers and by native mass spectrometry data. CONCLUSIONS/SIGNIFICANCE: The subunit number of the TRAP oligomer can be manipulated by introducing deletion or addition mutations at the subunit interface. An analysis of available and emerging structural data on alternative oligomeric states indicates that the same principles may also apply to the subunit number of other circular assemblies suggesting that the deletion/addition approach could be used generally to engineer transitions between different oligomeric states.

-	<!--	+	How to Change the Oligomeric State of a Circular Protein Assembly: Switch from 11-Subunit to 12-Subunit TRAP Suggests a General Mechanism.,Chen CS, Smits C, Dodson GG, Shevtsov MB, Merlino N, Gollnick P, Antson AA PLoS One. 2011;6(10):e25296. Epub 2011 Oct 3. PMID:21984911<ref>PMID:21984911</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3zzq"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3zzq\| PDB=3zzq \| SCENE= }}~~	+

-	~~===ENGINEERED 12-SUBUNIT BACILLUS SUBTILIS TRP RNA-BINDING ATTENUATION PROTEIN (TRAP)===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21984911}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21984911 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21984911}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3zzq]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021984911<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Antson, A A.]]		[[Category: Antson, A A.]]

OCA at 07:22, 30 October 2011

OCA — Sun, 30 Oct 2011 07:22:21 GMT

←Older revision		Revision as of 07:22, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zzq.png\|left\|200px]]

-	The ~~entry 3zzq~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zzq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zzq\| PDB=3zzq \| SCENE= }}

-	~~Authors: Chen, C., Smits, C., Dodson, G.G., Shevtsov, M.B., Merlino, N., Gollnick, P., Antson, A.A.~~	+	===ENGINEERED 12-SUBUNIT BACILLUS SUBTILIS TRP RNA-BINDING ATTENUATION PROTEIN (TRAP)===

-	~~Description~~: ~~Engineered 12~~-~~subunit~~ Bacillus subtilis ~~trp RNA~~-~~binding attenuation protein (TRAP)~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21984911}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21984911 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21984911}}
		+
		+	==About this Structure==
		+	[[3zzq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021984911</ref><references group="xtra"/>
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Antson, A A.]]
		+	[[Category: Chen, C.]]
		+	[[Category: Dodson, G G.]]
		+	[[Category: Gollnick, P.]]
		+	[[Category: Merlino, N.]]
		+	[[Category: Shevtsov, M B.]]
		+	[[Category: Smits, C.]]
		+	[[Category: Protein engineering]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription regulation]]

OCA: Protected "3zzq" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2011 06:09:57 GMT

Protected "3zzq" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:09, 14 September 2011

OCA: New page: '''Unreleased structure''' The entry 3zzq is ON HOLD until Paper Publication Authors: Chen, C., Smits, C., Dodson, G.G., Shevtsov, M.B., Merlino, N., Gollnick, P., Antson, A.A. Descrip...

OCA — Wed, 14 Sep 2011 06:09:56 GMT

New page: '''Unreleased structure''' The entry 3zzq is ON HOLD until Paper Publication Authors: Chen, C., Smits, C., Dodson, G.G., Shevtsov, M.B., Merlino, N., Gollnick, P., Antson, A.A. Descrip...

New page

'''Unreleased structure'''

The entry 3zzq is ON HOLD until Paper Publication

Authors: Chen, C., Smits, C., Dodson, G.G., Shevtsov, M.B., Merlino, N., Gollnick, P., Antson, A.A.

Description: Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)