471d - Revision history

OCA at 19:06, 10 December 2016

OCA — Sat, 10 Dec 2016 19:06:22 GMT

←Older revision		Revision as of 19:06, 10 December 2016
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		+
	==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA==		==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA==
	<StructureSection load='471d' size='340' side='right' caption='[[471d]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='471d' size='340' side='right' caption='[[471d]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB], [http://www.ebi.ac.uk/pdbsum/471d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://pdbe.org/471d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB], [http://www.ebi.ac.uk/pdbsum/471d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=471d ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 471d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:38, 9 December 2014

OCA — Tue, 09 Dec 2014 17:38:22 GMT

←Older revision		Revision as of 17:38, 9 December 2014
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-	[[~~Image:~~471d.~~png~~\|~~left~~\|~~200px~~]]	+	==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA==
		+	<StructureSection load='471d' size='340' side='right' caption='[[471d]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[471d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471D FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB], [http://www.ebi.ac.uk/pdbsum/471d PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.

-	~~{{STRUCTURE_471d\| PDB=471d \| SCENE= }}~~	+	Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA.,Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355<ref>PMID:10360355</ref>

-	~~===CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10360355}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[471d]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=471D OCA].~~	+	</StructureSection>
-		+	[[Category: Cook, P D]]
-	==~~Reference~~==	+	[[Category: Egli, M]]
-	<~~ref group="xtra">PMID:010360355<~~/~~ref~~><~~references group="xtra"~~/>	+	[[Category: Inamati, G]]
-	[[Category: Cook, P D.]]	+	[[Category: Minasov, G]]
-	[[Category: Egli, M.]]	+	[[Category: Teplova, M]]
-	[[Category: Inamati, G.]]	+	[[Category: Tereshko, V]]
-	[[Category: Minasov, G.]]	+
-	[[Category: Teplova, M.]]	+
-	[[Category: Tereshko, V.]]	+
	[[Category: Ribonucleic acid]]		[[Category: Ribonucleic acid]]
	[[Category: Rna]]		[[Category: Rna]]
	[[Category: Rna dodecamer duplex]]		[[Category: Rna dodecamer duplex]]

OCA: Protected "471d" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2013 09:19:00 GMT

Protected "471d" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 16 January 2013

OCA at 09:18, 16 January 2013

OCA — Wed, 16 Jan 2013 09:18:59 GMT

←Older revision		Revision as of 09:18, 16 January 2013
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-	{{Seed}}
	[[Image:471d.png\|left\|200px]]		[[Image:471d.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_471d", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_471d\| PDB=471d \| SCENE= }}		{{STRUCTURE_471d\| PDB=471d \| SCENE= }}

	===CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA===		===CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10360355}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10360355 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10360355}}		{{ABSTRACT_PUBMED_10360355}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].	+	[[471d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].

	==Reference==		==Reference==
-	~~Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~10360355 10360355]~~	+	<ref group="xtra">PMID:010360355</ref><references group="xtra"/>
	[[Category: Cook, P D.]]		[[Category: Cook, P D.]]
	[[Category: Egli, M.]]		[[Category: Egli, M.]]
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	[[Category: Tereshko, V.]]		[[Category: Tereshko, V.]]
	[[Category: Ribonucleic acid]]		[[Category: Ribonucleic acid]]
		+	[[Category: Rna]]
	[[Category: Rna dodecamer duplex]]		[[Category: Rna dodecamer duplex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:24:27 2008''

OCA at 10:24, 3 July 2008

OCA — Thu, 03 Jul 2008 10:24:27 GMT

←Older revision		Revision as of 10:24, 3 July 2008
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-	[[Image:471d.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:471d.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_471d\| PDB=471d \| SCENE= }}		{{STRUCTURE_471d\| PDB=471d \| SCENE= }}

-	~~'''~~CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA~~'''~~	+	===CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA===


-	~~==Overview==~~	+	<!--
-	2'-O-~~(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA)~~, the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (~~CGCGAAUUCGCG) was determined~~ at 1.~~7 A resolution~~. ~~In the majority of the MOE substituents~~, ~~the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups~~ is ~~consistent with~~ the ~~improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA~~. ~~A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE~~-~~RNA may explain its high nuclease resistance.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_10360355}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10360355 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10360355}}

	==About this Structure==		==About this Structure==
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	[[Category: Ribonucleic acid]]		[[Category: Ribonucleic acid]]
	[[Category: Rna dodecamer duplex]]		[[Category: Rna dodecamer duplex]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:19:30 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:24:27 2008''

OCA at 19:19, 4 May 2008

OCA — Sun, 04 May 2008 19:19:31 GMT

←Older revision		Revision as of 19:19, 4 May 2008
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	[[Image:471d.gif\|left\|200px]]		[[Image:471d.gif\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 471d \|SIZE=350\|CAPTION= <~~scene ~~name='initialview01'>471d</scene>~~, ~~resolution 2~~.~~70Å~~	+	The line below this paragraph, containing "STRUCTURE_471d", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=A44:2'~~-O-METHYOXYETHYL-ADENOSINE+5'-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2'-O-METHYOXYETHYL-CYTIDINE-5'-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2'-O-METHYOXYETHYL-GUANOSINE-5'-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U36:2'-O-METHYOXYETHYL-URIDINE-5'-MONOPHOSPHATE'>U36</scene>	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	\|~~ACTIVITY~~=	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_471d\| PDB=471d \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://www.ebi.ac.uk/pdbsum/471d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB]</span>	+
-	}}	+

	'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA'''		'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA'''
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	==About this Structure==		==About this Structure==
-	~~471D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].	+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].

	==Reference==		==Reference==
	Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360355 10360355]		Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360355 10360355]
-	[[Category: Protein complex]]
	[[Category: Cook, P D.]]		[[Category: Cook, P D.]]
	[[Category: Egli, M.]]		[[Category: Egli, M.]]
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	[[Category: Teplova, M.]]		[[Category: Teplova, M.]]
	[[Category: Tereshko, V.]]		[[Category: Tereshko, V.]]
-	[[Category: ~~2'-o-(2-methoxyethyl) ribose]]~~	+	[[Category: Ribonucleic acid]]
-	~~[[Category: ribonucleic~~ acid]]	+	[[Category: Rna dodecamer duplex]]
-	[[Category: ~~rna~~ dodecamer duplex]]	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:30 2008''
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:37:38 2008''	+

OCA at 02:37, 31 March 2008

OCA — Mon, 31 Mar 2008 02:37:39 GMT

←Older revision		Revision as of 02:37, 31 March 2008
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	\|PDB= 471d \|SIZE=350\|CAPTION= <scene name='initialview01'>471d</scene>, resolution 2.70Å		\|PDB= 471d \|SIZE=350\|CAPTION= <scene name='initialview01'>471d</scene>, resolution 2.70Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>	+	\|LIGAND= <scene name='pdbligand=A44:2'-O-METHYOXYETHYL-ADENOSINE+5'-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2'-O-METHYOXYETHYL-CYTIDINE-5'-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2'-O-METHYOXYETHYL-GUANOSINE-5'-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U36:2'-O-METHYOXYETHYL-URIDINE-5'-MONOPHOSPHATE'>U36</scene>
	\|ACTIVITY=		\|ACTIVITY=
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=471d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=471d OCA], [http://www.ebi.ac.uk/pdbsum/471d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=471d RCSB]</span>
	}}		}}

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	[[Category: Teplova, M.]]		[[Category: Teplova, M.]]
	[[Category: Tereshko, V.]]		[[Category: Tereshko, V.]]
-	[[Category: MG]]
	[[Category: 2'-o-(2-methoxyethyl) ribose]]		[[Category: 2'-o-(2-methoxyethyl) ribose]]
	[[Category: ribonucleic acid]]		[[Category: ribonucleic acid]]
	[[Category: rna dodecamer duplex]]		[[Category: rna dodecamer duplex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19:08~~:37 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:37:38 2008''

OCA at 17:08, 20 March 2008

OCA — Thu, 20 Mar 2008 17:08:38 GMT

←Older revision		Revision as of 17:08, 20 March 2008
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-	[[Image:471d.gif\|left\|200px]]~~<br /><applet load~~="471d~~" size~~="350~~" color~~=~~"white" frame="true" align="right" spinBox="true"~~	+	[[Image:471d.gif\|left\|200px]]
-	~~caption~~="471d, resolution 2.70Å" />	+
-	'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA'''~~<br />~~	+	{{Structure
		+	\|PDB= 471d \|SIZE=350\|CAPTION= <scene name='initialview01'>471d</scene>, resolution 2.70Å
		+	\|SITE=
		+	\|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
		+	\|ACTIVITY=
		+	\|GENE=
		+	}}
		+
		+	'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA'''
		+

	==Overview==		==Overview==
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	==About this Structure==		==About this Structure==
-	471D is a [~~http://en.wikipedia.org/wiki/Protein_complex~~ Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ~~] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].	+	471D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].

	==Reference==		==Reference==
-	Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~10360355 10360355]	+	Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360355 10360355]
	[[Category: Protein complex]]		[[Category: Protein complex]]
	[[Category: Cook, P D.]]		[[Category: Cook, P D.]]
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	[[Category: rna dodecamer duplex]]		[[Category: rna dodecamer duplex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21~~ 19:12:28 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:37 2008''

OCA at 17:12, 21 February 2008

OCA — Thu, 21 Feb 2008 17:12:33 GMT

←Older revision		Revision as of 17:12, 21 February 2008
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	==Overview==		==Overview==
-	2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with, promising features for antisense applications. Compared with, phosphorothioate DNA (PS-DNA), the MOE modification offers improved, nuclease resistance, enhanced RNA affinity, improved cellular uptake and, intestinal absorption, reduced toxicity and immune stimulation. The, crystal structure of a fully modified MOE-RNA dodecamer duplex, (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the, MOE substituents, the torsion angle around the ethylene alkyl chain, assumes a gauche conformation. The conformational preorganization of the, MOE groups is consistent with the improved RNA affinity and the extensive, hydration of the substituents could play a role in the improved cellular, uptake of MOE-RNA. A specific hydration pattern that bridges substituent, and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its, high nuclease resistance.	+	2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.

	==About this Structure==		==About this Structure==
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	Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10360355 10360355]		Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10360355 10360355]
	[[Category: Protein complex]]		[[Category: Protein complex]]
-	[[Category: Cook, P.D.]]	+	[[Category: Cook, P D.]]
	[[Category: Egli, M.]]		[[Category: Egli, M.]]
	[[Category: Inamati, G.]]		[[Category: Inamati, G.]]
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	[[Category: rna dodecamer duplex]]		[[Category: rna dodecamer duplex]]

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OCA: New page: 200px
'''CRYSTAL STRUCTURE AN...
OCA — Tue, 29 Jan 2008 19:44:50 GMT

New page: 200px<br /><applet load="471d" size="350" color="white" frame="true" align="right" spinBox="true" caption="471d, resolution 2.70Å" /> '''CRYSTAL STRUCTURE AN...

New page
[[Image:471d.gif|left|200px]]<br /><applet load="471d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="471d, resolution 2.70Å" />
'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA'''<br />

==Overview==
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with, promising features for antisense applications. Compared with, phosphorothioate DNA (PS-DNA), the MOE modification offers improved, nuclease resistance, enhanced RNA affinity, improved cellular uptake and, intestinal absorption, reduced toxicity and immune stimulation. The, crystal structure of a fully modified MOE-RNA dodecamer duplex, (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the, MOE substituents, the torsion angle around the ethylene alkyl chain, assumes a gauche conformation. The conformational preorganization of the, MOE groups is consistent with the improved RNA affinity and the extensive, hydration of the substituents could play a role in the improved cellular, uptake of MOE-RNA. A specific hydration pattern that bridges substituent, and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its, high nuclease resistance.

==About this Structure==
471D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=471D OCA].

==Reference==
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10360355 10360355]
[[Category: Protein complex]]
[[Category: Cook, P.D.]]
[[Category: Egli, M.]]
[[Category: Inamati, G.]]
[[Category: Minasov, G.]]
[[Category: Teplova, M.]]
[[Category: Tereshko, V.]]
[[Category: MG]]
[[Category: 2'-o-(2-methoxyethyl) ribose]]
[[Category: ribonucleic acid]]
[[Category: rna dodecamer duplex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:44:49 2008''