4a5k - Revision history

OCA at 11:19, 20 December 2023

OCA — Wed, 20 Dec 2023 11:19:54 GMT

←Older revision		Revision as of 11:19, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4a5k' size='340' side='right'caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='4a5k' size='340' side='right'caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A5K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A5K FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.76Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [https://pdbe.org/4a5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [https://www.ebi.ac.uk/pdbsum/4a5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [https://pdbe.org/4a5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [https://www.ebi.ac.uk/pdbsum/4a5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SLM1_YEAST SLM1_YEAST]] Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.<ref>PMID:15372071</ref> <ref>PMID:15689497</ref> <ref>PMID:16959779</ref> <ref>PMID:16738335</ref>	+	[https://www.uniprot.org/uniprot/SLM1_YEAST SLM1_YEAST] Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.<ref>PMID:15372071</ref> <ref>PMID:15689497</ref> <ref>PMID:16959779</ref> <ref>PMID:16738335</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Anand, K~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Gavin, A C~~]]	+	[[Category: Anand K]]
-	[[Category: ~~Inositol phosphate]]~~	+	[[Category: Gavin AC]]
-	~~[[Category: Non-canonical binding site]]~~	+
-	~~[[Category: Phosphoserine]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 07:46, 18 August 2022

OCA — Thu, 18 Aug 2022 07:46:20 GMT

←Older revision		Revision as of 07:46, 18 August 2022
Line 3:		Line 3:
	<StructureSection load='4a5k' size='340' side='right'caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='4a5k' size='340' side='right'caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4A5K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A5K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [~~http~~://pdbe.org/4a5k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4a5k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [https://pdbe.org/4a5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [https://www.ebi.ac.uk/pdbsum/4a5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SLM1_YEAST SLM1_YEAST]] Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.<ref>PMID:15372071</ref> <ref>PMID:15689497</ref> <ref>PMID:16959779</ref> <ref>PMID:16738335</ref>	+	[[https://www.uniprot.org/uniprot/SLM1_YEAST SLM1_YEAST]] Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.<ref>PMID:15372071</ref> <ref>PMID:15689497</ref> <ref>PMID:16959779</ref> <ref>PMID:16738335</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Anand, K]]		[[Category: Anand, K]]
	[[Category: Gavin, A C]]		[[Category: Gavin, A C]]

OCA at 09:32, 6 March 2019

OCA — Wed, 06 Mar 2019 09:32:27 GMT

←Older revision		Revision as of 09:32, 6 March 2019
Line 1:		Line 1:

	==Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site==		==Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site==
-	<StructureSection load='4a5k' size='340' side='right' caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>	+	<StructureSection load='4a5k' size='340' side='right'caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>

OCA at 20:40, 5 August 2016

OCA — Fri, 05 Aug 2016 20:40:45 GMT

←Older revision		Revision as of 20:40, 5 August 2016
Line 1:		Line 1:
		+
	==Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site==		==Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site==
	<StructureSection load='4a5k' size='340' side='right' caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='4a5k' size='340' side='right' caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [http://www.ebi.ac.uk/pdbsum/4a5k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [http://pdbe.org/4a5k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [http://www.ebi.ac.uk/pdbsum/4a5k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4a5k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Anand, K]]		[[Category: Anand, K]]
	[[Category: Gavin, A C]]		[[Category: Gavin, A C]]

OCA at 05:42, 25 December 2014

OCA — Thu, 25 Dec 2014 05:42:03 GMT

←Older revision		Revision as of 05:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [http://www.ebi.ac.uk/pdbsum/4a5k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [http://www.ebi.ac.uk/pdbsum/4a5k PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SLM1_YEAST SLM1_YEAST]] Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.<ref>PMID:15372071</ref> <ref>PMID:15689497</ref> <ref>PMID:16959779</ref> <ref>PMID:16738335</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Anand, K.]]	+	[[Category: Anand, K]]
-	[[Category: Gavin, A C.]]	+	[[Category: Gavin, A C]]
	[[Category: Inositol phosphate]]		[[Category: Inositol phosphate]]
	[[Category: Non-canonical binding site]]		[[Category: Non-canonical binding site]]
	[[Category: Phosphoserine]]		[[Category: Phosphoserine]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 08:20, 5 June 2014

OCA — Thu, 05 Jun 2014 08:20:48 GMT

←Older revision		Revision as of 08:20, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4a5k.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site==
		+	<StructureSection load='4a5k' size='340' side='right' caption='[[4a5k]], [[Resolution\|resolution]] 1.76Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a6f\|4a6f]], [[4a6h\|4a6h]], [[4a6k\|4a6k]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a5k RCSB], [http://www.ebi.ac.uk/pdbsum/4a5k PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Pleckstrin homology (PH) domains are common membrane-targeting modules and their best characterized ligands are a set of important signaling lipids that include phosphatidylinositol phosphates (PtdInsPs). PH domains recognize PtdInsPs through two distinct mechanisms that use different binding pockets on opposite sides of the beta-strands 1 and 2: i) a canonical binding site delimited by the beta1-beta2 and beta3-beta4loops and ii) a non-canonical binding site bordered by the beta1-beta2 and beta5-beta6loops. The PH domain-containing protein Slm1 from budding yeast Saccharomyces cerevisiae is required for actin cytoskeleton polarization and cell growth. We recently reported that this PH domain binds PtdInsPs and phosphorylated sphingolipids in a cooperative manner. PRINCIPAL FINDINGS: To study the structural basis for the Slm1-PH domain (Slm1-PH) specificity, we co-crystallized this domain with different soluble compounds that have structures analogous to anionic lipid head groups of reported Slm1 ligands: inositol 4-phosphate, which mimics phosphatidylinositol-4-phosphate (PtdIns(4)P), and phosphoserine as a surrogate for dihydrosphingosine 1-phosphate (DHS1-P). We found electron densities for the ligands within the so-called non-canonical binding site. An additional positively charged surface that contacts a phosphate group was identified next to the canonical binding site. CONCLUSIONS: Our results suggest that Slm1-PH utilizes a non-canonical binding site to bind PtdInsPs, similar to that described for the PH domains of beta-spectrin, Tiam1 and ArhGAP9. Additionally, Slm1-PH may have retained an active canonical site. We propose that the presence of both a canonical and a non-canonical binding pocket in Slm1-PH may account for the cooperative binding to PtdInsPs and DHS-1P.

-	<!--	+	Structural Analyses of the Slm1-PH Domain Demonstrate Ligand Binding in the Non-Canonical Site.,Anand K, Maeda K, Gavin AC PLoS One. 2012;7(5):e36526. Epub 2012 May 4. PMID:22574179<ref>PMID:22574179</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4a5k"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_4a5k\| PDB=4a5k \| SCENE= }}~~	+

-	~~===Structural analyses~~ of ~~Slm1-PH domain demonstrate ligand binding in~~ the ~~non-canonical site===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22574179}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22574179 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22574179}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4a5k]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022574179<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Anand, K.]]		[[Category: Anand, K.]]

OCA at 06:08, 13 June 2012

OCA — Wed, 13 Jun 2012 06:08:15 GMT

←Older revision		Revision as of 06:08, 13 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4a5k.jpg\|left\|200px]]

-	The ~~entry 4a5k~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4a5k", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4a5k\| PDB=4a5k \| SCENE= }}

-	~~Authors: Anand, K., Gavin, A.C.~~	+	===Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site===

-	~~Description: Structural analyses of Slm1~~-~~PH domain demonstrate ligand binding in~~ the ~~non~~-canonical site	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22574179}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22574179 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22574179}}
		+
		+	==About this Structure==
		+	[[4a5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5K OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022574179</ref><references group="xtra"/>
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Anand, K.]]
		+	[[Category: Gavin, A C.]]
		+	[[Category: Inositol phosphate]]
		+	[[Category: Non-canonical binding site]]
		+	[[Category: Phosphoserine]]
		+	[[Category: Signaling protein]]

OCA at 18:47, 2 May 2012

OCA — Wed, 02 May 2012 18:47:55 GMT

←Older revision		Revision as of 18:47, 2 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4a5k is ON HOLD ~~until sometime in the future~~	+	The entry 4a5k is ON HOLD

	Authors: Anand, K., Gavin, A.C.		Authors: Anand, K., Gavin, A.C.

	Description: Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site		Description: Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site

OCA at 08:41, 25 April 2012

OCA — Wed, 25 Apr 2012 08:41:00 GMT

←Older revision		Revision as of 08:41, 25 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4a5k is ON HOLD	+	The entry 4a5k is ON HOLD until sometime in the future

	Authors: Anand, K., Gavin, A.C.		Authors: Anand, K., Gavin, A.C.

	Description: Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site		Description: Structural analyses of Slm1-PH domain demonstrate ligand binding in the non-canonical site

OCA: Protected "4a5k" [edit=sysop:move=sysop]

OCA — Wed, 02 Nov 2011 08:05:05 GMT

Protected "4a5k" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:05, 2 November 2011