4a8h - Revision history

OCA at 11:21, 20 December 2023

OCA — Wed, 20 Dec 2023 11:21:49 GMT

←Older revision		Revision as of 11:21, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4a8h' size='340' side='right'caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4a8h' size='340' side='right'caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A8H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4a8t\|4a8t]], [[4a8p\|4a8p]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [https://pdbe.org/4a8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [https://www.ebi.ac.uk/pdbsum/4a8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a8h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [https://pdbe.org/4a8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [https://www.ebi.ac.uk/pdbsum/4a8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PTC_ENTFA PTC_ENTFA]] Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.<ref>PMID:4621632</ref>	+	[https://www.uniprot.org/uniprot/PTC_ENTFA PTC_ENTFA] Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.<ref>PMID:4621632</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~proteiformis thiercelin and jouhaud 1903~~]]	+	[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Putrescine carbamoyltransferase]]~~	+	[[Category: Gil-Ortiz F]]
-	[[Category: Gil-Ortiz, F]]	+	[[Category: Polo LM]]
-	[[Category: Polo~~, L M~~]]	+	[[Category: Rubio V]]
-	[[Category: Rubio, V]]	+
-	~~[[Category: Agmatine deiminase route]]~~	+
-	~~[[Category: Ornithine]]~~	+
-	~~[[Category: Ornithine arginine deiminase]]~~	+
-	~~[[Category: Palo]]~~	+
-	~~[[Category: Papu]]~~	+
-	~~[[Category: Phosphonoacetylputrescine]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:52, 18 August 2022

OCA — Thu, 18 Aug 2022 07:52:06 GMT

←Older revision		Revision as of 07:52, 18 August 2022
Line 3:		Line 3:
	<StructureSection load='4a8h' size='340' side='right'caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4a8h' size='340' side='right'caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4A8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A8H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a8t\|4a8t]], [[4a8p\|4a8p]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4a8t\|4a8t]], [[4a8p\|4a8p]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [~~http~~://pdbe.org/4a8h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4a8h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4a8h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [https://pdbe.org/4a8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [https://www.ebi.ac.uk/pdbsum/4a8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PTC_ENTFA PTC_ENTFA]] Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.<ref>PMID:4621632</ref>	+	[[https://www.uniprot.org/uniprot/PTC_ENTFA PTC_ENTFA]] Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.<ref>PMID:4621632</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Putrescine carbamoyltransferase]]		[[Category: Putrescine carbamoyltransferase]]
	[[Category: Gil-Ortiz, F]]		[[Category: Gil-Ortiz, F]]

OCA at 09:41, 6 March 2019

OCA — Wed, 06 Mar 2019 09:41:54 GMT

←Older revision		Revision as of 09:41, 6 March 2019
Line 1:		Line 1:

	==Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine==		==Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine==
-	<StructureSection load='4a8h' size='340' side='right' caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4a8h' size='340' side='right'caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A8H FirstGlance]. <br>		<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A8H FirstGlance]. <br>

OCA at 07:49, 5 August 2016

OCA — Fri, 05 Aug 2016 07:49:03 GMT

←Older revision		Revision as of 07:49, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine==		==Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine==
	<StructureSection load='4a8h' size='340' side='right' caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4a8h' size='340' side='right' caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterococcus_faecalis Enterococcus faecalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A8H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a8t\|4a8t]], [[4a8p\|4a8p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a8t\|4a8t]], [[4a8p\|4a8p]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [http://www.ebi.ac.uk/pdbsum/4a8h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [http://pdbe.org/4a8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [http://www.ebi.ac.uk/pdbsum/4a8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a8h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4a8h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~faecalis~~]]	+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Putrescine carbamoyltransferase]]		[[Category: Putrescine carbamoyltransferase]]
	[[Category: Gil-Ortiz, F]]		[[Category: Gil-Ortiz, F]]

OCA at 17:01, 25 December 2014

OCA — Thu, 25 Dec 2014 17:01:53 GMT

←Older revision		Revision as of 17:01, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4a8h.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine==
		+	<StructureSection load='4a8h' size='340' side='right' caption='[[4a8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A8H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PUW:N-(PHOSPHONOACETYL)-PUTRESCINE'>PUW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a8t\|4a8t]], [[4a8p\|4a8p]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Putrescine_carbamoyltransferase Putrescine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.6 2.1.3.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a8h RCSB], [http://www.ebi.ac.uk/pdbsum/4a8h PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PTC_ENTFA PTC_ENTFA]] Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine.<ref>PMID:4621632</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transcarbamylases reversibly transfer a carbamyl group from carbamylphosphate (CP) to an amine. Although aspartate transcarbamylase and ornithine transcarbamylase (OTC) are well characterized, little was known about putrescine transcarbamylase (PTC), the enzyme that generates CP for ATP production in the fermentative catabolism of agmatine. We demonstrate that PTC (from Enterococcus faecalis), in addition to using putrescine, can utilize L-ornithine as a poor substrate. Crystal structures at 2.5 A and 2.0 A resolutions of PTC bound to its respective bisubstrate analog inhibitors for putrescine and ornithine use, N-(phosphonoacetyl)-putrescine and delta-N-(phosphonoacetyl)-L-ornithine, shed light on PTC preference for putrescine. Except for a highly prominent C-terminal helix that projects away and embraces an adjacent subunit, PTC closely resembles OTCs, suggesting recent divergence of the two enzymes. Since differences between the respective 230 and SMG loops of PTC and OTC appeared to account for the differential preference of these enzymes for putrescine and ornithine, we engineered the 230-loop of PTC to make it to resemble the SMG loop of OTCs, increasing the activity with ornithine and greatly decreasing the activity with putrescine. We also examined the role of the C-terminal helix that appears a constant and exclusive PTC trait. The enzyme lacking this helix remained active but the PTC trimer stability appeared decreased, since some of the enzyme eluted as monomers from a gel filtration column. In addition, truncated PTC tended to aggregate to hexamers, as shown both chromatographically and by X-ray crystallography. Therefore, the extra C-terminal helix plays a dual role: it stabilizes the PTC trimer and, by shielding helix 1 of an adjacent subunit, it prevents the supratrimeric oligomerizations of obscure significance observed with some OTCs. Guided by the structural data we identify signature traits that permit easy and unambiguous annotation of PTC sequences.

-	~~<!-~~-	+	New insight into the transcarbamylase family: the structure of putrescine transcarbamylase, a key catalyst for fermentative utilization of agmatine.,Polo LM, Gil-Ortiz F, Cantin A, Rubio V PLoS One. 2012;7(2):e31528. Epub 2012 Feb 20. PMID:22363663<ref>PMID:22363663</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4a8h"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_4a8h\| PDB=4a8h \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22363663}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22363663 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22363663}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4a8h]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022363663<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
	[[Category: Putrescine carbamoyltransferase]]		[[Category: Putrescine carbamoyltransferase]]
-	[[Category: Gil-Ortiz, F.]]	+	[[Category: Gil-Ortiz, F]]
-	[[Category: Polo, L M.]]	+	[[Category: Polo, L M]]
-	[[Category: Rubio, V.]]	+	[[Category: Rubio, V]]
	[[Category: Agmatine deiminase route]]		[[Category: Agmatine deiminase route]]
	[[Category: Ornithine]]		[[Category: Ornithine]]

OCA at 05:51, 14 March 2012

OCA — Wed, 14 Mar 2012 05:51:28 GMT

←Older revision		Revision as of 05:51, 14 March 2012
Line 1:		Line 1:
-	[[Image:4a8h.~~jpg~~\|left\|200px]]	+	[[Image:4a8h.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine===		===Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22363663}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22363663 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22363663}}

	==About this Structure==		==About this Structure==
	[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA].		[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022363663</ref><references group="xtra"/>
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
	[[Category: Putrescine carbamoyltransferase]]		[[Category: Putrescine carbamoyltransferase]]

OCA at 06:56, 15 February 2012

OCA — Wed, 15 Feb 2012 06:56:29 GMT

←Older revision		Revision as of 06:56, 15 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4a8h.jpg\|left\|200px]]

-	The ~~entry 4a8h~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4a8h", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4a8h\| PDB=4a8h \| SCENE= }}

-	~~Authors: Polo, L.M., Gil~~-~~Ortiz, F., Rubio, V.~~	+	===Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine===

-	~~Description: Crystal~~ structure ~~of putrescine transcarbamylase~~ from Enterococcus faecalis ~~with N~~-~~(phosphonoacetyl)~~-~~putrescine~~	+
		+	==About this Structure==
		+	[[4a8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A8H OCA].
		+	[[Category: Enterococcus faecalis]]
		+	[[Category: Putrescine carbamoyltransferase]]
		+	[[Category: Gil-Ortiz, F.]]
		+	[[Category: Polo, L M.]]
		+	[[Category: Rubio, V.]]
		+	[[Category: Agmatine deiminase route]]
		+	[[Category: Ornithine]]
		+	[[Category: Ornithine arginine deiminase]]
		+	[[Category: Palo]]
		+	[[Category: Papu]]
		+	[[Category: Phosphonoacetylputrescine]]
		+	[[Category: Transferase]]

OCA at 07:44, 7 December 2011

OCA — Wed, 07 Dec 2011 07:44:24 GMT

←Older revision		Revision as of 07:44, 7 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4a8h is ON HOLD	+	The entry 4a8h is ON HOLD until Paper Publication

	Authors: Polo, L.M., Gil-Ortiz, F., Rubio, V.		Authors: Polo, L.M., Gil-Ortiz, F., Rubio, V.

	Description: Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine		Description: Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine

OCA: Protected "4a8h" [edit=sysop:move=sysop]

OCA — Wed, 30 Nov 2011 06:00:35 GMT

Protected "4a8h" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:00, 30 November 2011

OCA: New page: '''Unreleased structure''' The entry 4a8h is ON HOLD Authors: Polo, L.M., Gil-Ortiz, F., Rubio, V. Description: Crystal structure of putrescine transcarbamylase from Enterococcus faeca...

OCA — Wed, 30 Nov 2011 06:00:34 GMT

New page: '''Unreleased structure''' The entry 4a8h is ON HOLD Authors: Polo, L.M., Gil-Ortiz, F., Rubio, V. Description: Crystal structure of putrescine transcarbamylase from Enterococcus faeca...

New page

'''Unreleased structure'''

The entry 4a8h is ON HOLD

Authors: Polo, L.M., Gil-Ortiz, F., Rubio, V.

Description: Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine