4aaf - Revision history

OCA at 11:23, 20 December 2023

OCA — Wed, 20 Dec 2023 11:23:03 GMT

←Older revision		Revision as of 11:23, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4aaf' size='340' side='right'caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4aaf' size='340' side='right'caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AAF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AAF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [https://pdbe.org/4aaf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [https://www.ebi.ac.uk/pdbsum/4aaf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aaf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [https://pdbe.org/4aaf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [https://www.ebi.ac.uk/pdbsum/4aaf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aaf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.	+	[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlre~~]]	+	[[Category: Chlamydomonas reinhardtii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abramo, M D]]	+	[[Category: Synthetic construct]]
-	[[Category: Duchateau, P]]	+	[[Category: D'Abramo M]]
-	[[Category: Epinat~~, J C~~]]	+	[[Category: Duchateau P]]
-	[[Category: Gervasio~~, F L~~]]	+	[[Category: Epinat JC]]
-	[[Category: Grizot, S]]	+	[[Category: Gervasio FL]]
-	[[Category: Marenchino, M]]	+	[[Category: Grizot S]]
-	[[Category: Molina, R]]	+	[[Category: Marenchino M]]
-	[[Category: Montoya, G]]	+	[[Category: Molina R]]
-	[[Category: Prieto, J]]	+	[[Category: Montoya G]]
-	[[Category: Redondo, P]]	+	[[Category: Prieto J]]
-	[[Category: Stella, S]]	+	[[Category: Redondo P]]
-	[[Category: Valton, J]]	+	[[Category: Stella S]]
-	~~[[Category: Gene targeting]]~~	+	[[Category: Valton J]]
-	~~[[Category: Homing endonuclease]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Protein-dna interaction~~]]	+

OCA at 07:55, 18 August 2022

OCA — Thu, 18 Aug 2022 07:55:49 GMT

←Older revision		Revision as of 07:55, 18 August 2022
Line 3:		Line 3:
	<StructureSection load='4aaf' size='340' side='right'caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4aaf' size='340' side='right'caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AAF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AAF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [~~http~~://pdbe.org/4aaf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4aaf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4aaf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [https://pdbe.org/4aaf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [https://www.ebi.ac.uk/pdbsum/4aaf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aaf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.	+	[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
		+	[[Category: Large Structures]]
	[[Category: Abramo, M D]]		[[Category: Abramo, M D]]
	[[Category: Duchateau, P]]		[[Category: Duchateau, P]]

OCA at 09:15, 6 March 2019

OCA — Wed, 06 Mar 2019 09:15:26 GMT

←Older revision		Revision as of 09:15, 6 March 2019
Line 1:		Line 1:

	==Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')==		==Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')==
-	<StructureSection load='4aaf' size='340' side='right' caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4aaf' size='340' side='right'caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAF FirstGlance]. <br>

OCA at 05:55, 5 August 2016

OCA — Fri, 05 Aug 2016 05:55:35 GMT

←Older revision		Revision as of 05:55, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')==		==Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')==
	<StructureSection load='4aaf' size='340' side='right' caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4aaf' size='340' side='right' caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [http://www.ebi.ac.uk/pdbsum/4aaf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [http://pdbe.org/4aaf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [http://www.ebi.ac.uk/pdbsum/4aaf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aaf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4aaf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlamydomonas reinhardtii~~]]	+	[[Category: Chlre]]
	[[Category: Abramo, M D]]		[[Category: Abramo, M D]]
	[[Category: Duchateau, P]]		[[Category: Duchateau, P]]

OCA at 16:18, 25 December 2014

OCA — Thu, 25 Dec 2014 16:18:43 GMT

←Older revision		Revision as of 16:18, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4aaf.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')==
		+	<StructureSection load='4aaf' size='340' side='right' caption='[[4aaf]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g9z\|1g9z]], [[1bp7\|1bp7]], [[2vbl\|2vbl]], [[4aae\|4aae]], [[2vbo\|2vbo]], [[1mow\|1mow]], [[1u0d\|1u0d]], [[4aad\|4aad]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1n3e\|1n3e]], [[4aab\|4aab]], [[1af5\|1af5]], [[2vbj\|2vbj]], [[1g9y\|1g9y]], [[1n3f\|1n3f]], [[2vbn\|2vbn]], [[1u0c\|1u0c]], [[4aag\|4aag]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aaf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aaf RCSB], [http://www.ebi.ac.uk/pdbsum/4aaf PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Homing endonucleases represent protein scaffolds that provide powerful tools for genome manipulation, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. The basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. Protein-DNA interaction engineering of homing endonucleases has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Protein-DNA interface studies have been focused mostly on specific contacts between amino acid side chains and bases to redesign the binding interface. However, it has been shown that 4 bp in the central DNA sequence of the 22-bp substrate of a homing endonuclease (I-CreI), which do not show specific protein-DNA interactions, is not devoid of content information. Here, we analyze the mechanism of target discrimination in this substrate region by the I-CreI protein, determining how it can occur independently of the specific protein-DNA interactions. Our data suggest the important role of indirect readout in this substrate region, opening the possibility for a fully rational search of new target sequences, thus improving the development of redesigned enzymes for therapeutic and biotechnological applications.

-	~~{{STRUCTURE_4aaf\| PDB=4aaf \| SCENE= }}~~	+	Non-specific protein-DNA interactions control I-CreI target binding and cleavage.,Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Charles Epinat J, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G Nucleic Acids Res. 2012 Apr 11. PMID:22495931<ref>PMID:22495931</ref>

-	~~===Crystal structure~~ of the ~~mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22495931}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4aaf]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].~~	+

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022495931<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
-	[[Category: Abramo, M D.]]	+	[[Category: Abramo, M D]]
-	[[Category: Duchateau, P.]]	+	[[Category: Duchateau, P]]
-	[[Category: Epinat, J C.]]	+	[[Category: Epinat, J C]]
-	[[Category: Gervasio, F L.]]	+	[[Category: Gervasio, F L]]
-	[[Category: Grizot, S.]]	+	[[Category: Grizot, S]]
-	[[Category: Marenchino, M.]]	+	[[Category: Marenchino, M]]
-	[[Category: Molina, R.]]	+	[[Category: Molina, R]]
-	[[Category: Montoya, G.]]	+	[[Category: Montoya, G]]
-	[[Category: Prieto, J.]]	+	[[Category: Prieto, J]]
-	[[Category: Redondo, P.]]	+	[[Category: Redondo, P]]
-	[[Category: Stella, S.]]	+	[[Category: Stella, S]]
-	[[Category: Valton, J.]]	+	[[Category: Valton, J]]
	[[Category: Gene targeting]]		[[Category: Gene targeting]]
	[[Category: Homing endonuclease]]		[[Category: Homing endonuclease]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Protein-dna interaction]]		[[Category: Protein-dna interaction]]

OCA at 18:49, 20 October 2012

OCA — Sat, 20 Oct 2012 18:49:05 GMT

←Older revision		Revision as of 18:49, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[4aaf]] is a 4 chain structure ~~of [[Endonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].	+	[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].

	==See Also==		==See Also==

OCA at 05:19, 27 July 2012

OCA — Fri, 27 Jul 2012 05:19:47 GMT

←Older revision		Revision as of 05:19, 27 July 2012
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-	[[Image:4aaf.~~jpg~~\|left\|200px]]	+	[[Image:4aaf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4aaf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4aaf\| PDB=4aaf \| SCENE= }}		{{STRUCTURE_4aaf\| PDB=4aaf \| SCENE= }}

	===Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')===		===Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22495931}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22495931 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22495931}}		{{ABSTRACT_PUBMED_22495931}}

	==About this Structure==		==About this Structure==
-	[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].	+	[[4aaf]] is a 4 chain structure of [[Endonuclease]] with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]

	==Reference==		==Reference==

OCA at 19:15, 2 May 2012

OCA — Wed, 02 May 2012 19:15:09 GMT

←Older revision		Revision as of 19:15, 2 May 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4aaf.jpg\|left\|200px]]

-	The ~~entry 4aaf~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4aaf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4aaf\| PDB=4aaf \| SCENE= }}

-	~~Authors: Molina~~, R., ~~Redondo, P., Stella, S., Marenchino, M., D~~'~~Abramo, M., Gervasio, F., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.~~	+	===Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')===

-	~~Description: Crystal structure of the mutant D75N I~~-~~CreI in complex with an altered target (~~The ~~four central bases~~, ~~2NN region~~, ~~are composed by TGCA from 5'~~ to 3')	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22495931}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22495931 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22495931}}
		+
		+	==About this Structure==
		+	[[4aaf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022495931</ref><references group="xtra"/>
		+	[[Category: Chlamydomonas reinhardtii]]
		+	[[Category: Abramo, M D.]]
		+	[[Category: Duchateau, P.]]
		+	[[Category: Epinat, J C.]]
		+	[[Category: Gervasio, F L.]]
		+	[[Category: Grizot, S.]]
		+	[[Category: Marenchino, M.]]
		+	[[Category: Molina, R.]]
		+	[[Category: Montoya, G.]]
		+	[[Category: Prieto, J.]]
		+	[[Category: Redondo, P.]]
		+	[[Category: Stella, S.]]
		+	[[Category: Valton, J.]]
		+	[[Category: Gene targeting]]
		+	[[Category: Homing endonuclease]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Protein-dna interaction]]

OCA at 07:00, 28 December 2011

OCA — Wed, 28 Dec 2011 07:00:00 GMT

←Older revision		Revision as of 07:00, 28 December 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4aaf is ON HOLD	+	The entry 4aaf is ON HOLD until Paper Publication

	Authors: Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.		Authors: Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.

	Description: Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')		Description: Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')

OCA: Protected "4aaf" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2011 11:22:13 GMT

Protected "4aaf" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:22, 14 December 2011