4akp - Revision history

OCA at 11:29, 20 December 2023

OCA — Wed, 20 Dec 2023 11:29:18 GMT

←Older revision		Revision as of 11:29, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4akp' size='340' side='right'caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4akp' size='340' side='right'caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AKP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AKP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [https://pdbe.org/4akp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [https://www.ebi.ac.uk/pdbsum/4akp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4akp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [https://pdbe.org/4akp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [https://www.ebi.ac.uk/pdbsum/4akp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4akp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.	+	[https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU] Involved in brown pigmentation during sporogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835]]~~	+	[[Category: Bacillus subtilis]]
-	~~[[Category: Laccase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bento, I]]	+	[[Category: Bento I]]
-	[[Category: Chen, Z]]	+	[[Category: Chen Z]]
-	[[Category: Durao, P]]	+	[[Category: Durao P]]
-	[[Category: Hildebrandt, P]]	+	[[Category: Hildebrandt P]]
-	[[Category: Lindley~~, P F~~]]	+	[[Category: Lindley PF]]
-	[[Category: Martins~~, L O~~]]	+	[[Category: Martins LO]]
-	[[Category: Pereira~~, M M~~]]	+	[[Category: Pereira MM]]
-	[[Category: Silva~~, C S~~]]	+	[[Category: Silva CS]]
-	[[Category: Todorovic, S]]	+	[[Category: Todorovic S]]
-	~~[[Category: Multi-copper oxidase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Trinuclear cluster oxygen reduction~~]]	+

OCA at 05:37, 25 August 2022

OCA — Thu, 25 Aug 2022 05:37:23 GMT

←Older revision		Revision as of 05:37, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4akp' size='340' side='right'caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4akp' size='340' side='right'caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AKP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AKP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Laccase Laccase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [~~http~~://pdbe.org/4akp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4akp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4akp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [https://pdbe.org/4akp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [https://www.ebi.ac.uk/pdbsum/4akp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4akp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.	+	[[https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[~~CotA laccase~~\|~~CotA laccase~~]]	+	*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Laccase]]		[[Category: Laccase]]
		+	[[Category: Large Structures]]
	[[Category: Bento, I]]		[[Category: Bento, I]]
	[[Category: Chen, Z]]		[[Category: Chen, Z]]

OCA at 09:03, 6 March 2019

OCA — Wed, 06 Mar 2019 09:03:55 GMT

←Older revision		Revision as of 09:03, 6 March 2019
Line 1:		Line 1:

	==Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant==		==Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant==
-	<StructureSection load='4akp' size='340' side='right' caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4akp' size='340' side='right'caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
Line 28:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Laccase]]		[[Category: Laccase]]
	[[Category: Bento, I]]		[[Category: Bento, I]]

OCA at 10:05, 4 August 2016

OCA — Thu, 04 Aug 2016 10:05:45 GMT

←Older revision		Revision as of 10:05, 4 August 2016
Line 1:		Line 1:
		+
	==Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant==		==Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant==
	<StructureSection load='4akp' size='340' side='right' caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4akp' size='340' side='right' caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [http://www.ebi.ac.uk/pdbsum/4akp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [http://pdbe.org/4akp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [http://www.ebi.ac.uk/pdbsum/4akp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4akp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4akp" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Laccase]]		[[Category: Laccase]]
	[[Category: Bento, I]]		[[Category: Bento, I]]

OCA at 08:11, 25 December 2014

OCA — Thu, 25 Dec 2014 08:11:05 GMT

←Older revision		Revision as of 08:11, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [http://www.ebi.ac.uk/pdbsum/4akp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [http://www.ebi.ac.uk/pdbsum/4akp PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:11, 9 December 2014

OCA — Tue, 09 Dec 2014 15:11:43 GMT

←Older revision		Revision as of 15:11, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4akp.~~png~~\|~~left~~\|~~200px~~]]	+	==Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant==
		+	<StructureSection load='4akp' size='340' side='right' caption='[[4akp]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gsk\|1gsk]], [[1of0\|1of0]], [[1uvw\|1uvw]], [[1w6l\|1w6l]], [[1w6w\|1w6w]], [[1w8e\|1w8e]], [[2bhf\|2bhf]], [[2x87\|2x87]], [[2x88\|2x88]], [[4a66\|4a66]], [[4a67\|4a67]], [[4a68\|4a68]], [[4ako\|4ako]], [[4akq\|4akq]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4akp RCSB], [http://www.ebi.ac.uk/pdbsum/4akp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The multicopper oxidases couple the one-electron oxidation of four substrate molecules to the four electron reductive cleavage of the O-O bond of dioxygen. This reduction takes place at the trinuclear copper centre of the enzyme and the dioxygen approaches this centre through an entrance channel. In this channel, an acidic residue plays a key role in steering the dioxygen to the trinuclear copper site, providing protons for the catalytic reaction and giving overall stability to this site. In this study, the role of the Glu(498) residue, located within the entrance channel to the trinuclear copper centre, has been investigated in the binding and reduction of dioxygen by the CotA-laccase from Bacillus subtilis. The absence of an acidic group at the 498 residue, as in the E498T and E498L mutants, results in a severe catalytic impairment, higher than 99%, for the phenolic and non-phenolic substrates tested. The replacement of this glutamate by aspartate leads to an activity that is around 10% relative to that of the wild-type. Furthermore, while this latter mutant shows a similar K(m) value for dioxygen, the E498T and E498L mutants show a decreased affinity, when compared to the wild-type. X-ray structural and spectroscopic analysis (UV-visible, electron paramagnetic resonance and resonance Raman) reveal perturbations of the structural properties of the catalytic centres in the Glu(498) mutants when compared to the wild-type protein. Overall, the results strongly suggest that Glu(498) plays a key role in the protonation events that occur at the trinuclear centre and in its stabilization, controlling therefore the binding of dioxygen and its further reduction.

-	~~{{STRUCTURE_4akp\| PDB=4akp \| SCENE= }}~~	+	The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis.,Chen Z, Durao P, Silva CS, Pereira MM, Todorovic S, Hildebrandt P, Bento I, Lindley PF, Martins LO Dalton Trans. 2010 Mar 21;39(11):2875-82. Epub 2010 Feb 5. PMID:20200715<ref>PMID:20200715</ref>

-	~~===Mutations in~~ the ~~neighbourhood~~ of ~~CotA-laccase trinuclear site: E498T mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_20200715}}~~	+	==See Also==
-		+	*[[CotA laccase\|CotA laccase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4akp~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:020200715<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Laccase]]		[[Category: Laccase]]
-	[[Category: Bento, I.]]	+	[[Category: Bento, I]]
-	[[Category: Chen, Z.]]	+	[[Category: Chen, Z]]
-	[[Category: Durao, P.]]	+	[[Category: Durao, P]]
-	[[Category: Hildebrandt, P.]]	+	[[Category: Hildebrandt, P]]
-	[[Category: Lindley, P F.]]	+	[[Category: Lindley, P F]]
-	[[Category: Martins, L O.]]	+	[[Category: Martins, L O]]
-	[[Category: Pereira, M M.]]	+	[[Category: Pereira, M M]]
-	[[Category: Silva, C S.]]	+	[[Category: Silva, C S]]
-	[[Category: Todorovic, S.]]	+	[[Category: Todorovic, S]]
	[[Category: Multi-copper oxidase]]		[[Category: Multi-copper oxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Trinuclear cluster oxygen reduction]]		[[Category: Trinuclear cluster oxygen reduction]]

OCA at 09:51, 21 October 2012

OCA — Sun, 21 Oct 2012 09:51:05 GMT

←Older revision		Revision as of 09:51, 21 October 2012
Line 9:		Line 9:
	==About this Structure==		==About this Structure==
	[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].		[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].
-
-	==See Also==
-	*[[Spore coat protein\|Spore coat protein]]

	==Reference==		==Reference==

OCA at 14:30, 20 October 2012

OCA — Sat, 20 Oct 2012 14:30:34 GMT

←Older revision		Revision as of 14:30, 20 October 2012
Line 1:		Line 1:
-	[[Image:4akp.~~jpg~~\|left\|200px]]	+	[[Image:4akp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4akp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4akp\| PDB=4akp \| SCENE= }}		{{STRUCTURE_4akp\| PDB=4akp \| SCENE= }}

	===Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant===		===Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20200715}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20200715 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20200715}}		{{ABSTRACT_PUBMED_20200715}}

	==About this Structure==		==About this Structure==
	[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].		[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].
		+
		+	==See Also==
		+	*[[Spore coat protein\|Spore coat protein]]

	==Reference==		==Reference==

OCA at 06:34, 14 March 2012

OCA — Wed, 14 Mar 2012 06:34:21 GMT

←Older revision		Revision as of 06:34, 14 March 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4akp.jpg\|left\|200px]]

-	The ~~entry 4akp~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4akp", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4akp\| PDB=4akp \| SCENE= }}

-	~~Authors~~: ~~Silva, C.S., Chen, Z., Durao, P., Pereira, M.M., Todorovic, S., Hildebrandt, P., Martins, L.O., Lindley, P.F., Bento, I.~~	+	===Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant===

-	~~Description~~: ~~Mutations in~~ the ~~neighbourhood of CotA~~-~~laccase trinuclear site~~: ~~E498T mutant~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20200715}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20200715 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20200715}}
		+
		+	==About this Structure==
		+	[[4akp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:020200715</ref><references group="xtra"/>
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Laccase]]
		+	[[Category: Bento, I.]]
		+	[[Category: Chen, Z.]]
		+	[[Category: Durao, P.]]
		+	[[Category: Hildebrandt, P.]]
		+	[[Category: Lindley, P F.]]
		+	[[Category: Martins, L O.]]
		+	[[Category: Pereira, M M.]]
		+	[[Category: Silva, C S.]]
		+	[[Category: Todorovic, S.]]
		+	[[Category: Multi-copper oxidase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Trinuclear cluster oxygen reduction]]

OCA: Protected "4akp" [edit=sysop:move=sysop]

OCA — Thu, 08 Mar 2012 05:22:35 GMT

Protected "4akp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 8 March 2012