4alr - Revision history

OCA at 11:29, 20 December 2023

OCA — Wed, 20 Dec 2023 11:29:49 GMT

←Older revision		Revision as of 11:29, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4alr' size='340' side='right'caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='4alr' size='340' side='right'caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ALR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ALR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.49Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[4a02\|4a02]]~~, ~~[[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [https://pdbe.org/4alr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [https://www.ebi.ac.uk/pdbsum/4alr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4alr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [https://pdbe.org/4alr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [https://www.ebi.ac.uk/pdbsum/4alr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4alr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LCHMO_ENTFA LCHMO_ENTFA] Involved in chitin degradation. Catalyzes the oxidative cleavage of glycosidic bonds in both alpha- and beta-chitin via a copper-dependent mechanism, leading to oxidized chitooligosaccharides with a dominance of even-numbered products. Acts synergistically with the chitinase EfChi18A, and combining the two enzymes leads to rapid and complete depolymerization of crystalline chitin, especially with beta-chitin as a substrate. Is likely involved in a chitin degradation pathway that allows E.faecalis V583 to grow on chitin as a carbon source.<ref>PMID:22210154</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~proteiformis thiercelin and jouhaud 1903~~]]	+	[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eijsink, V]]	+	[[Category: Eijsink V]]
-	[[Category: Gudmundsson, M]]	+	[[Category: Gudmundsson M]]
-	[[Category: Ishida, T]]	+	[[Category: Ishida T]]
-	[[Category: Momeni~~, M H~~]]	+	[[Category: Momeni MH]]
-	[[Category: Sandgren, M]]	+	[[Category: Sandgren M]]
-	[[Category: Vaaje-Kolstad, G]]	+	[[Category: Vaaje-Kolstad G]]
-	[[Category: Wu, M]]	+	[[Category: Wu M]]
-	~~[[Category: Chitin binding protein]]~~	+
-	~~[[Category: Chitin degradation]]~~	+
-	~~[[Category: Chitin-binding protein]]~~	+
-	~~[[Category: Microspectrophotometry x-ray induced photo reduction~~]]	+

OCA at 05:38, 25 August 2022

OCA — Thu, 25 Aug 2022 05:38:20 GMT

←Older revision		Revision as of 05:38, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4alr' size='340' side='right'caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='4alr' size='340' side='right'caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ALR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ALR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [~~http~~://pdbe.org/4alr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4alr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4alr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [https://pdbe.org/4alr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [https://www.ebi.ac.uk/pdbsum/4alr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4alr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:36, 10 May 2019

OCA — Fri, 10 May 2019 12:36:30 GMT

←Older revision		Revision as of 12:36, 10 May 2019
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Eijsink, V]]		[[Category: Eijsink, V]]
	[[Category: Gudmundsson, M]]		[[Category: Gudmundsson, M]]

OCA at 09:34, 6 March 2019

OCA — Wed, 06 Mar 2019 09:34:27 GMT

←Older revision		Revision as of 09:34, 6 March 2019
Line 1:		Line 1:

-	==X-Ray photoreduction of Polysaccharide ~~monooxigenase~~ CBM33==	+	==X-Ray photoreduction of Polysaccharide monooxygenase CBM33==
-	<StructureSection load='4alr' size='340' side='right' caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>	+	<StructureSection load='4alr' size='340' side='right'caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALR FirstGlance]. <br>

OCA at 10:31, 11 August 2016

OCA — Thu, 11 Aug 2016 10:31:30 GMT

←Older revision		Revision as of 10:31, 11 August 2016
Line 1:		Line 1:
		+
	==X-Ray photoreduction of Polysaccharide monooxigenase CBM33==		==X-Ray photoreduction of Polysaccharide monooxigenase CBM33==
	<StructureSection load='4alr' size='340' side='right' caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='4alr' size='340' side='right' caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [http://www.ebi.ac.uk/pdbsum/4alr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [http://pdbe.org/4alr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [http://www.ebi.ac.uk/pdbsum/4alr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4alr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4alr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:55, 5 January 2015

OCA — Mon, 05 Jan 2015 14:55:15 GMT

←Older revision		Revision as of 14:55, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [http://www.ebi.ac.uk/pdbsum/4alr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [http://www.ebi.ac.uk/pdbsum/4alr PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
-	[[Category: Eijsink, V.]]	+	[[Category: Eijsink, V]]
-	[[Category: Gudmundsson, M.]]	+	[[Category: Gudmundsson, M]]
-	[[Category: Ishida, T.]]	+	[[Category: Ishida, T]]
-	[[Category: Momeni, M H.]]	+	[[Category: Momeni, M H]]
-	[[Category: Sandgren, M.]]	+	[[Category: Sandgren, M]]
-	[[Category: Vaaje-Kolstad, G.]]	+	[[Category: Vaaje-Kolstad, G]]
-	[[Category: Wu, M.]]	+	[[Category: Wu, M]]
	[[Category: Chitin binding protein]]		[[Category: Chitin binding protein]]
	[[Category: Chitin degradation]]		[[Category: Chitin degradation]]
	[[Category: Chitin-binding protein]]		[[Category: Chitin-binding protein]]
	[[Category: Microspectrophotometry x-ray induced photo reduction]]		[[Category: Microspectrophotometry x-ray induced photo reduction]]

OCA at 06:26, 13 August 2014

OCA — Wed, 13 Aug 2014 06:26:11 GMT

←Older revision		Revision as of 06:26, 13 August 2014
Line 13:		Line 13:
	Structural and electronic snapshots during the transition from a Cu(II) to Cu(I) metal center of a lytic polysaccharide monooxygenase by X-ray photo-reduction.,Gudmundsson M, Kim S, Wu M, Ishida T, Haddad Momeni M, Vaaje-Kolstad G, Lundberg D, Royant A, Stahlberg J, Eijsink VG, Beckham GT, Sandgren M J Biol Chem. 2014 May 14. pii: jbc.M114.563494. PMID:24828494<ref>PMID:24828494</ref>		Structural and electronic snapshots during the transition from a Cu(II) to Cu(I) metal center of a lytic polysaccharide monooxygenase by X-ray photo-reduction.,Gudmundsson M, Kim S, Wu M, Ishida T, Haddad Momeni M, Vaaje-Kolstad G, Lundberg D, Royant A, Stahlberg J, Eijsink VG, Beckham GT, Sandgren M J Biol Chem. 2014 May 14. pii: jbc.M114.563494. PMID:24828494<ref>PMID:24828494</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 07:01, 28 May 2014

OCA — Wed, 28 May 2014 07:01:46 GMT

←Older revision		Revision as of 07:01, 28 May 2014
Line 1:		Line 1:
-	[[~~Image:~~4alr.~~jpg~~\|~~left~~\|~~200px~~]]	+	==X-Ray photoreduction of Polysaccharide monooxigenase CBM33==
		+	<StructureSection load='4alr' size='340' side='right' caption='[[4alr]], [[Resolution\|resolution]] 1.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4a02\|4a02]], [[4alc\|4alc]], [[4ale\|4ale]], [[4alq\|4alq]], [[4alt\|4alt]], [[4als\|4als]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4alr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4alr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4alr RCSB], [http://www.ebi.ac.uk/pdbsum/4alr PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lytic polysaccharide monooxygenases (LPMOs) are a recently discovered class of enzymes that employ a copper-mediated, oxidative mechanism to cleave glycosidic bonds. The LPMO catalytic mechanism likely requires that molecular oxygen first binds to Cu(I), but the oxidation state in many reported LPMO structures is ambiguous, and the changes in the LPMO active site required to accommodate both oxidation states of copper have not been fully elucidated. Here, a helical X-ray diffraction method with minimal X-ray dose was used to solve the crystal structure of a chitin-specific LPMO from Enterococcus faecalis (EfaCBM33A) in the Cu(II)-bound form. Subsequently, the crystal was X-ray photo-reduced, which revealed structural changes associated with the conversion from the initial Cu(II)-oxidized form with two coordinated water molecules, which adopts a trigonal bipyramidal geometry, to a reduced Cu(I) form in a T-shaped geometry with no coordinated water molecules. A comprehensive survey of Cu(II) and Cu(I) structures in the Cambridge Structural Database unambiguously shows that the geometries observed in the least and most reduced structures reflect binding of Cu(II) and Cu(I), respectively. Quantum mechanical calculations of the oxidized and reduced active sites reveal little change in the electronic structure of the active site measured by the active site partial charges. Together with a previous theoretical investigation of a fungal LPMO, this suggests significant functional plasticity in LPMO active sites. Overall, this study provides molecular snapshots along the reduction process to activate the LPMO catalytic machinery, and provides a general method for solving LPMO structures in both copper oxidation states.

-	~~{{STRUCTURE_4alr\| PDB=4alr \| SCENE= }}~~	+	Structural and electronic snapshots during the transition from a Cu(II) to Cu(I) metal center of a lytic polysaccharide monooxygenase by X-ray photo-reduction.,Gudmundsson M, Kim S, Wu M, Ishida T, Haddad Momeni M, Vaaje-Kolstad G, Lundberg D, Royant A, Stahlberg J, Eijsink VG, Beckham GT, Sandgren M J Biol Chem. 2014 May 14. pii: jbc.M114.563494. PMID:24828494<ref>PMID:24828494</ref>

-	~~===X-Ray photoreduction~~ of ~~Polysaccharide monooxigenase CBM33===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[4alr]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA].~~	+	__TOC__
-	[[Category: Enterococcus ~~faecalis~~]]	+	</StructureSection>
		+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Eijsink, V.]]		[[Category: Eijsink, V.]]
	[[Category: Gudmundsson, M.]]		[[Category: Gudmundsson, M.]]

OCA at 14:52, 27 February 2013

OCA — Wed, 27 Feb 2013 14:52:44 GMT

←Older revision		Revision as of 14:52, 27 February 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4alr.jpg\|left\|200px]]

-	~~The entry~~ 4alr ~~is ON HOLD~~	+	{{STRUCTURE_4alr\| PDB=4alr \| SCENE= }}

-	~~Authors: Gudmundsson, M., Wu, M., Ishida, T., Momeni, M.H., Vaaje~~-~~Kolstad, G., Eijsink, V., Sandgren, M.~~	+	===X-Ray photoreduction of Polysaccharide monooxigenase CBM33===

-	~~Description~~: X-~~Ray photoreduction of Polysaccharide monooxigenase CBM33~~	+
		+	==About this Structure==
		+	[[4alr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALR OCA].
		+	[[Category: Enterococcus faecalis]]
		+	[[Category: Eijsink, V.]]
		+	[[Category: Gudmundsson, M.]]
		+	[[Category: Ishida, T.]]
		+	[[Category: Momeni, M H.]]
		+	[[Category: Sandgren, M.]]
		+	[[Category: Vaaje-Kolstad, G.]]
		+	[[Category: Wu, M.]]
		+	[[Category: Chitin binding protein]]
		+	[[Category: Chitin degradation]]
		+	[[Category: Chitin-binding protein]]
		+	[[Category: Microspectrophotometry x-ray induced photo reduction]]

OCA: Protected "4alr" [edit=sysop:move=sysop]

OCA — Wed, 14 Mar 2012 06:08:17 GMT

Protected "4alr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:08, 14 March 2012