4arf - Revision history

OCA at 11:33, 20 December 2023

OCA — Wed, 20 Dec 2023 11:33:51 GMT

←Older revision		Revision as of 11:33, 20 December 2023
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	<StructureSection load='4arf' size='340' side='right'caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4arf' size='340' side='right'caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg~~ and ~~seguin 1916~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hathewaya_histolytica Hathewaya histolytica] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~IP8~~:~~ISOPENTENYL~~+~~PHOSPHATE~~'>~~IP8~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[4ar1\|4ar1]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [https://pdbe.org/4arf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [https://www.ebi.ac.uk/pdbsum/4arf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4arf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [https://pdbe.org/4arf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [https://www.ebi.ac.uk/pdbsum/4arf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4arf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/COLH_HATHI COLH_HATHI] Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (PubMed:3002446). The full-length protein has collagenase activity, while both the 116 kDa and 98 kDa forms act on gelatin (PubMed:7961400). In vitro digestion of soluble calf skin collagen fibrils requires both ColG and ColH; ColG forms missing the second collagen-binding domain is also synergistic with ColH, although their overall efficiency is decreased (PubMed:18374061, PubMed:22099748). Digestion of collagen requires Ca(2+) and is inhibited by EDTA (PubMed:9452493). The activator domain (residues 119-388) and catalytic subdomain (330-601) open and close around substrate allowing digestion when the protein is closed (PubMed:23703618).<ref>PMID:18374061</ref> <ref>PMID:18937627</ref> <ref>PMID:22099748</ref> <ref>PMID:23703618</ref> <ref>PMID:24125730</ref> <ref>PMID:28820255</ref> <ref>PMID:3002446</ref> <ref>PMID:7961400</ref> <ref>PMID:9452493</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus histolyticus weinberg and seguin 1916~~]]	+	[[Category: Hathewaya histolytica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Microbial collagenase~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Brandstetter, H]]	+	[[Category: Brandstetter H]]
-	[[Category: Eckhard, U]]	+	[[Category: Eckhard U]]
-	~~[[Category: Collagenolysis]]~~	+
-	~~[[Category: Hexxh]]~~	+
-	~~[[Category: Hydrolase-inhibitor complex]]~~	+
-	~~[[Category: Hydrolyse]]~~	+
-	~~[[Category: Metalloprotease~~]]	+

OCA at 05:43, 25 August 2022

OCA — Thu, 25 Aug 2022 05:43:46 GMT

←Older revision		Revision as of 05:43, 25 August 2022
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	<StructureSection load='4arf' size='340' side='right'caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4arf' size='340' side='right'caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ARF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ar1\|4ar1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ar1\|4ar1]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [~~http~~://pdbe.org/4arf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4arf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4arf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [https://pdbe.org/4arf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [https://www.ebi.ac.uk/pdbsum/4arf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4arf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:40, 29 January 2020

OCA — Wed, 29 Jan 2020 16:40:39 GMT

←Older revision		Revision as of 16:40, 29 January 2020
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	==CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.==		==CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.==
-	<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>	+	<StructureSection load='4arf' size='340' side='right'caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4arf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4arf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Collagenase (non-MMP)\|Collagenase (non-MMP)]]
		+	*[[Collagenase 3D structures\|Collagenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus histolyticus weinberg and seguin 1916]]		[[Category: Bacillus histolyticus weinberg and seguin 1916]]
		+	[[Category: Large Structures]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 06:33, 7 February 2018

OCA — Wed, 07 Feb 2018 06:33:56 GMT

←Older revision		Revision as of 06:33, 7 February 2018
Line 3:		Line 3:
	<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 4arf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4arf" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Collagenase (non-MMP)\|Collagenase (non-MMP)]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus histolyticus weinberg and seguin 1916]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 06:52, 9 March 2017

OCA — Thu, 09 Mar 2017 06:52:09 GMT

←Older revision		Revision as of 06:52, 9 March 2017
Line 3:		Line 3:
	<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus histolyticus weinberg and seguin 1916]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 00:02, 5 August 2016

OCA — Fri, 05 Aug 2016 00:02:04 GMT

←Older revision		Revision as of 00:02, 5 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.==		==CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.==
	<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_histolyticum Clostridium histolyticum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ar1\|4ar1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ar1\|4ar1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [http://www.ebi.ac.uk/pdbsum/4arf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [http://pdbe.org/4arf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [http://www.ebi.ac.uk/pdbsum/4arf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4arf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4arf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Collagenase (non-MMP)\|Collagenase (non-MMP)]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium histolyticum~~]]	+	[[Category: Bacillus histolyticus weinberg and seguin 1916]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 08:55, 21 December 2014

OCA — Sun, 21 Dec 2014 08:55:55 GMT

←Older revision		Revision as of 08:55, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4arf\| PDB=4arf \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.==
-	===CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.===	+	<StructureSection load='4arf' size='340' side='right' caption='[[4arf]], [[Resolution\|resolution]] 1.77Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23703618}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_histolyticum Clostridium histolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IP8:ISOPENTENYL+PHOSPHATE'>IP8</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ar1\|4ar1]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4arf RCSB], [http://www.ebi.ac.uk/pdbsum/4arf PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridial collagenases are among the most efficient enzymes to degrade by far the most predominant protein in the biosphere. Here we present crystal structures of the peptidases of three clostridial collagenase isoforms (ColG, ColH and ColT). The comparison of unliganded and liganded structures reveal a quaternary subdomain dynamics. In the unliganded ColH structure this globular dynamics is modulated by an aspartate switch motion that binds to the catalytic zinc. We further identified a calcium binding site in proximity to the catalytic zinc. Both ions are required for full activity, explaining why calcium critically affects the enzymatic activity of clostridial collagenases. Our studies further reveal that loops close to the active site thus serve as characteristic substrate selectivity filter. These elements explain the distinct peptidolytic and collagenolytic activities of these enzymes and provide a rational to engineer collagenases with customized substrate specificity as well as for inhibitor design.

-	~~==About this Structure==~~	+	Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T.,Eckhard U, Schonauer E, Brandstetter H J Biol Chem. 2013 May 23. PMID:23703618<ref>PMID:23703618</ref>
-	~~[[4arf]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_histolyticum Clostridium histolyticum]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023703618</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium histolyticum]]		[[Category: Clostridium histolyticum]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
-	[[Category: Brandstetter, H.]]	+	[[Category: Brandstetter, H]]
-	[[Category: Eckhard, U.]]	+	[[Category: Eckhard, U]]
	[[Category: Collagenolysis]]		[[Category: Collagenolysis]]
	[[Category: Hexxh]]		[[Category: Hexxh]]

OCA at 13:30, 19 June 2013

OCA — Wed, 19 Jun 2013 13:30:01 GMT

←Older revision		Revision as of 13:30, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4arf\| PDB=4arf \| SCENE= }}
		+	===CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.===
		+	{{ABSTRACT_PUBMED_23703618}}

-	~~The entry~~ 4arf is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4arf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_histolyticum Clostridium histolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARF OCA].

-	~~Authors~~: ~~Eckhard, U.,~~ Brandstetter, H.	+	==Reference==
-		+	<ref group="xtra">PMID:023703618</ref><references group="xtra"/><references/>
-	~~Description~~: ~~CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL~~-~~GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.~~	+	[[Category: Clostridium histolyticum]]
		+	[[Category: Microbial collagenase]]
		+	[[Category: Brandstetter, H.]]
		+	[[Category: Eckhard, U.]]
		+	[[Category: Collagenolysis]]
		+	[[Category: Hexxh]]
		+	[[Category: Hydrolase-inhibitor complex]]
		+	[[Category: Hydrolyse]]
		+	[[Category: Metalloprotease]]

OCA: Protected "4arf" [edit=sysop:move=sysop]

OCA — Wed, 02 May 2012 18:50:17 GMT

Protected "4arf" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:50, 2 May 2012

OCA: New page: '''Unreleased structure''' The entry 4arf is ON HOLD until Paper Publication Authors: Eckhard, U., Brandstetter, H. Description: CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENAS...

OCA — Wed, 02 May 2012 18:50:16 GMT

New page: '''Unreleased structure''' The entry 4arf is ON HOLD until Paper Publication Authors: Eckhard, U., Brandstetter, H. Description: CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENAS...

New page

'''Unreleased structure'''

The entry 4arf is ON HOLD until Paper Publication

Authors: Eckhard, U., Brandstetter, H.

Description: CRYSTAL STRUCTURE OF THE PEPTIDASE DOMAIN OF COLLAGENASE H FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH THE PEPTIDIC INHIBITOR ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 1.77 ANGSTROM RESOLUTION.