4atv - Revision history

OCA at 07:16, 1 May 2024

OCA — Wed, 01 May 2024 07:16:33 GMT

←Older revision		Revision as of 07:16, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='4atv' size='340' side='right'caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4atv' size='340' side='right'caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LMU:DODECYL~~-~~ALPHA-D-MALTOSIDE'>LMU</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>~~[[1zcd\|1zcd]]~~</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [https://pdbe.org/4atv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [https://www.ebi.ac.uk/pdbsum/4atv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [https://pdbe.org/4atv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [https://www.ebi.ac.uk/pdbsum/4atv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>	+	[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Sodium-proton antiporters rapidly exchange protons and sodium ions across the membrane to regulate intracellular pH, cell volume, and sodium concentration. How ion binding and release is coupled to the conformational changes associated with transport is not clear. Here, we report a crystal form of the prototypical sodium-proton antiporter NhaA from Escherichia coli in which the protein is seen as a dimer. In this new structure, we observe a salt bridge between an essential aspartic acid (Asp163) and a conserved lysine (Lys300). An equivalent salt bridge is present in the homologous transporter NapA, but not in the only other known crystal structure of NhaA, which provides the foundation of most existing structural models of electrogenic sodium-proton antiport. Molecular dynamics simulations show that the stability of the salt bridge is weakened by sodium ions binding to Asp164 and the neighboring Asp163. This suggests that the transport mechanism involves Asp163 switching between forming a salt bridge with Lys300 and interacting with the sodium ion. pKa calculations suggest that Asp163 is highly unlikely to be protonated when involved in the salt bridge. As it has been previously suggested that Asp163 is one of the two residues through which proton transport occurs, these results have clear implications to the current mechanistic models of sodium-proton antiport in NhaA.	+
-		+
-	Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.,Lee C, Yashiro S, Dotson DL, Uzdavinys P, Iwata S, Sansom MS, von Ballmoos C, Beckstein O, Drew D, Cameron AD J Gen Physiol. 2014 Dec;144(6):529-44. doi: 10.1085/jgp.201411219. PMID:25422503<ref>PMID:25422503</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4atv" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cameron~~, A D~~]]	+	[[Category: Cameron AD]]
-	[[Category: Drew, D]]	+	[[Category: Drew D]]
-	[[Category: Iwata, S]]	+	[[Category: Iwata S]]
-	[[Category: Lee, C]]	+	[[Category: Lee C]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Sodium proton antiporter]]~~	+
-	~~[[Category: Transporter~~]]	+

OCA at 05:46, 25 August 2022

OCA — Thu, 25 Aug 2022 05:46:06 GMT

←Older revision		Revision as of 05:46, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4atv' size='340' side='right'caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4atv' size='340' side='right'caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ATV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zcd\|1zcd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zcd\|1zcd]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [~~http~~://pdbe.org/4atv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4atv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4atv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [https://pdbe.org/4atv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [https://www.ebi.ac.uk/pdbsum/4atv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>	+	[[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:02, 29 January 2020

OCA — Wed, 29 Jan 2020 17:02:04 GMT

←Older revision		Revision as of 17:02, 29 January 2020
Line 1:		Line 1:

	==STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH==		==STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH==
-	<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='4atv' size='340' side='right'caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATV FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Cameron, A D]]		[[Category: Cameron, A D]]
	[[Category: Drew, D]]		[[Category: Drew, D]]

OCA at 13:27, 4 May 2017

OCA — Thu, 04 May 2017 13:27:22 GMT

←Older revision		Revision as of 13:27, 4 May 2017
Line 1:		Line 1:
		+
	==STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH==		==STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH==
	<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zcd\|1zcd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zcd\|1zcd]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [http://www.ebi.ac.uk/pdbsum/4atv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [http://pdbe.org/4atv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [http://www.ebi.ac.uk/pdbsum/4atv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4atv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4atv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:07, 29 July 2015

OCA — Wed, 29 Jul 2015 08:07:21 GMT

←Older revision		Revision as of 08:07, 29 July 2015
Line 2:		Line 2:
	<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>		[[http://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sodium-proton antiporters rapidly exchange protons and sodium ions across the membrane to regulate intracellular pH, cell volume, and sodium concentration. How ion binding and release is coupled to the conformational changes associated with transport is not clear. Here, we report a crystal form of the prototypical sodium-proton antiporter NhaA from Escherichia coli in which the protein is seen as a dimer. In this new structure, we observe a salt bridge between an essential aspartic acid (Asp163) and a conserved lysine (Lys300). An equivalent salt bridge is present in the homologous transporter NapA, but not in the only other known crystal structure of NhaA, which provides the foundation of most existing structural models of electrogenic sodium-proton antiport. Molecular dynamics simulations show that the stability of the salt bridge is weakened by sodium ions binding to Asp164 and the neighboring Asp163. This suggests that the transport mechanism involves Asp163 switching between forming a salt bridge with Lys300 and interacting with the sodium ion. pKa calculations suggest that Asp163 is highly unlikely to be protonated when involved in the salt bridge. As it has been previously suggested that Asp163 is one of the two residues through which proton transport occurs, these results have clear implications to the current mechanistic models of sodium-proton antiport in NhaA.
		+
		+	Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.,Lee C, Yashiro S, Dotson DL, Uzdavinys P, Iwata S, Sansom MS, von Ballmoos C, Beckstein O, Drew D, Cameron AD J Gen Physiol. 2014 Dec;144(6):529-44. doi: 10.1085/jgp.201411219. PMID:25422503<ref>PMID:25422503</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Cameron, A D]]		[[Category: Cameron, A D]]
	[[Category: Drew, D]]		[[Category: Drew, D]]

OCA at 10:23, 25 December 2014

OCA — Thu, 25 Dec 2014 10:23:09 GMT

←Older revision		Revision as of 10:23, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [http://www.ebi.ac.uk/pdbsum/4atv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [http://www.ebi.ac.uk/pdbsum/4atv PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:33, 21 December 2014

OCA — Sun, 21 Dec 2014 09:33:52 GMT

←Older revision		Revision as of 09:33, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4atv\| PDB=4atv \| SCENE= }}~~	+	==STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH==
-	===STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH===	+	<StructureSection load='4atv' size='340' side='right' caption='[[4atv]], [[Resolution\|resolution]] 3.50Å' scene=''>
-		+	== Structural highlights ==
-	==~~Function~~==	+	<table><tr><td colspan='2'>[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATV FirstGlance]. <br>
-	[[~~http://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI~~]] ~~Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons~~. ~~Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref~~>~~PMID:1645730</ref>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMU:DODECYL-ALPHA-D-MALTOSIDE'>LMU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	==~~About this Structure~~==	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zcd\|1zcd]]</td></tr>
-	[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA].	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4atv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4atv RCSB], [http://www.ebi.ac.uk/pdbsum/4atv PDBsum]</span></td></tr>
-		+	</table>
-	==~~Reference~~==	+	__TOC__
-	<~~references group~~="~~xtra~~"/><~~references~~/>	+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Cameron, A D.]]	+	[[Category: Cameron, A D]]
-	[[Category: Drew, D.]]	+	[[Category: Drew, D]]
-	[[Category: Iwata, S.]]	+	[[Category: Iwata, S]]
-	[[Category: Lee, C.]]	+	[[Category: Lee, C]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Sodium proton antiporter]]		[[Category: Sodium proton antiporter]]
	[[Category: Transporter]]		[[Category: Transporter]]

OCA at 15:49, 10 July 2013

OCA — Wed, 10 Jul 2013 15:49:16 GMT

←Older revision		Revision as of 15:49, 10 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4atv\| PDB=4atv \| SCENE= }}
		+	===STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH===

-	~~The entry 4atv~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>

-	~~Authors~~: ~~Drew, D~~.~~, Lee, C~~.~~, Iwata, S~~.~~, Cameron, A~~.D.	+	==About this Structure==
		+	[[4atv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATV OCA].

-	~~Description~~: ~~STRUCTURE OF~~ A ~~TRIPLE MUTANT OF THE NHAA DIMER~~, ~~CRYSTALLISED AT LOW PH~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Cameron, A D.]]
		+	[[Category: Drew, D.]]
		+	[[Category: Iwata, S.]]
		+	[[Category: Lee, C.]]
		+	[[Category: Membrane protein]]
		+	[[Category: Sodium proton antiporter]]
		+	[[Category: Transporter]]

OCA at 05:21, 30 May 2012

OCA — Wed, 30 May 2012 05:21:10 GMT

←Older revision		Revision as of 05:21, 30 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4atv is ON HOLD	+	The entry 4atv is ON HOLD until Paper Publication

	Authors: Drew, D., Lee, C., Iwata, S., Cameron, A.D.		Authors: Drew, D., Lee, C., Iwata, S., Cameron, A.D.

-	Description: ~~Structure of the NhaA dimer~~, ~~crystallised at low pH~~	+	Description: STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH

OCA: Protected "4atv" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2012 08:43:17 GMT

Protected "4atv" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 16 May 2012