4awn - Revision history

OCA at 11:37, 20 December 2023

OCA — Wed, 20 Dec 2023 11:37:19 GMT

←Older revision		Revision as of 11:37, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4awn' size='340' side='right'caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4awn' size='340' side='right'caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AWN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [https://pdbe.org/4awn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [https://www.ebi.ac.uk/pdbsum/4awn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4awn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [https://pdbe.org/4awn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [https://www.ebi.ac.uk/pdbsum/4awn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4awn ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).	+	[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deoxyribonuclease I~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bourne, Y]]	+	[[Category: Bourne Y]]
-	[[Category: Lazarus~~, R A~~]]	+	[[Category: Lazarus RA]]
-	[[Category: Noguere, C]]	+	[[Category: Noguere C]]
-	[[Category: Parsiegla, G]]	+	[[Category: Parsiegla G]]
-	[[Category: Santell, L]]	+	[[Category: Santell L]]
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Pulmozyme~~]]	+

OCA at 05:48, 25 August 2022

OCA — Thu, 25 Aug 2022 05:48:57 GMT

←Older revision		Revision as of 05:48, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4awn' size='340' side='right'caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4awn' size='340' side='right'caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AWN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [~~http~~://pdbe.org/4awn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4awn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4awn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [https://pdbe.org/4awn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [https://www.ebi.ac.uk/pdbsum/4awn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4awn ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry.	+	[[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).	+	[[https://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribonuclease I]]		[[Category: Deoxyribonuclease I]]
-	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]

OCA at 12:39, 10 May 2019

OCA — Fri, 10 May 2019 12:39:17 GMT

←Older revision		Revision as of 12:39, 10 May 2019
Line 27:		Line 27:
	[[Category: Deoxyribonuclease I]]		[[Category: Deoxyribonuclease I]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Lazarus, R A]]		[[Category: Lazarus, R A]]

OCA at 09:02, 6 March 2019

OCA — Wed, 06 Mar 2019 09:02:55 GMT

←Older revision		Revision as of 09:02, 6 March 2019
Line 1:		Line 1:

	==Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.==		==Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.==
-	<StructureSection load='4awn' size='340' side='right' caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='4awn' size='340' side='right'caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWN FirstGlance]. <br>

OCA at 02:50, 11 December 2016

OCA — Sun, 11 Dec 2016 02:50:32 GMT

←Older revision		Revision as of 02:50, 11 December 2016
Line 1:		Line 1:
		+
	==Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.==		==Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.==
	<StructureSection load='4awn' size='340' side='right' caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4awn' size='340' side='right' caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [http://www.ebi.ac.uk/pdbsum/4awn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [http://pdbe.org/4awn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [http://www.ebi.ac.uk/pdbsum/4awn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4awn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNAS1_HUMAN DNAS1_HUMAN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4awn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribonuclease I]]		[[Category: Deoxyribonuclease I]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Lazarus, R A]]		[[Category: Lazarus, R A]]

OCA at 15:08, 9 December 2014

OCA — Tue, 09 Dec 2014 15:08:59 GMT

←Older revision		Revision as of 15:08, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4awn.~~png~~\|~~left\|200px~~]]	+	==Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.==
		+	<StructureSection load='4awn' size='340' side='right' caption='[[4awn]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4awn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4awn RCSB], [http://www.ebi.ac.uk/pdbsum/4awn PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recombinant human DNase I (Pulmozyme, dornase alfa) is used for the treatment of cystic fibrosis where it improves lung function and reduces the number of exacerbations. The physiological mechanism of action is thought to involve the reduction of the viscoelasticity of cystic fibrosis sputum by hydrolyzing high concentrations of DNA into low-molecular mass fragments. Here we describe the 1.95 A resolution crystal structure of recombinant human DNase I (rhDNase I) in complex with magnesium and phosphate ions, both bound in the active site. Complementary mutagenesis data of rhDNase I coupled to a comprehensive structural analysis of the DNase I-like superfamily argue for the key catalytic role of Asn7, which is invariant among mammalian DNase I enzymes and members of this superfamily, through stabilization of the magnesium ion coordination sphere. Overall, our combined structural and mutagenesis data suggest the occurrence of a magnesium-assisted pentavalent phosphate transition state in human DNase I during catalysis, where Asp168 may play a key role as a general catalytic base.

-	~~{{STRUCTURE_4awn\| PDB=4awn \| SCENE= }}~~	+	The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily.,Parsiegla G, Noguere C, Santell L, Lazarus RA, Bourne Y Biochemistry. 2012 Dec 21;51(51):10250-8. doi: 10.1021/bi300873f. Epub 2012 Dec, 13. PMID:23215638<ref>PMID:23215638</ref>

-	~~===Structure~~ of ~~recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23215638}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4awn]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA].~~	+	</StructureSection>
	[[Category: Deoxyribonuclease I]]		[[Category: Deoxyribonuclease I]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Bourne, Y.]]	+	[[Category: Bourne, Y]]
-	[[Category: Lazarus, R A.]]	+	[[Category: Lazarus, R A]]
-	[[Category: Noguere, C.]]	+	[[Category: Noguere, C]]
-	[[Category: Parsiegla, G.]]	+	[[Category: Parsiegla, G]]
-	[[Category: Santell, L.]]	+	[[Category: Santell, L]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Pulmozyme]]		[[Category: Pulmozyme]]

OCA at 12:05, 23 January 2013

OCA — Wed, 23 Jan 2013 12:05:01 GMT

←Older revision		Revision as of 12:05, 23 January 2013
Line 1:		Line 1:
-	[[Image:4awn.~~jpg~~\|left\|200px]]	+	[[Image:4awn.png\|left\|200px]]

	{{STRUCTURE_4awn\| PDB=4awn \| SCENE= }}		{{STRUCTURE_4awn\| PDB=4awn \| SCENE= }}

OCA at 08:12, 9 January 2013

OCA — Wed, 09 Jan 2013 08:12:19 GMT

←Older revision		Revision as of 08:12, 9 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4awn.jpg\|left\|200px]]

-	~~The entry~~ 4awn ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4awn\| PDB=4awn \| SCENE= }}

-	~~Authors: Parsiegla, G., Noguere, C., Santell, L., Lazarus, R.A., Bourne, Y~~.	+	===Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.===

-	~~Description:~~ Structure ~~of recombinant human DNase I (rhDNaseI) in complex~~ with ~~Magnesium and Phosphate~~.	+	{{ABSTRACT_PUBMED_23215638}}
		+
		+	==About this Structure==
		+	[[4awn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWN OCA].
		+	[[Category: Deoxyribonuclease I]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Bourne, Y.]]
		+	[[Category: Lazarus, R A.]]
		+	[[Category: Noguere, C.]]
		+	[[Category: Parsiegla, G.]]
		+	[[Category: Santell, L.]]
		+	[[Category: Endonuclease]]
		+	[[Category: Hydrolase]]
		+	[[Category: Pulmozyme]]

OCA at 05:15, 27 June 2012

OCA — Wed, 27 Jun 2012 05:15:30 GMT

←Older revision		Revision as of 05:15, 27 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4awn is ON HOLD	+	The entry 4awn is ON HOLD until Paper Publication

	Authors: Parsiegla, G., Noguere, C., Santell, L., Lazarus, R.A., Bourne, Y.		Authors: Parsiegla, G., Noguere, C., Santell, L., Lazarus, R.A., Bourne, Y.

	Description: Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.		Description: Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.

OCA: Protected "4awn" [edit=sysop:move=sysop]

OCA — Wed, 13 Jun 2012 05:39:38 GMT

Protected "4awn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 13 June 2012