4ax8 - Revision history

OCA at 10:54, 9 May 2024

OCA — Thu, 09 May 2024 10:54:53 GMT

←Older revision		Revision as of 10:54, 9 May 2024
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	<StructureSection load='4ax8' size='340' side='right'caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4ax8' size='340' side='right'caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [https://pdbe.org/4ax8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ax8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ax8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [https://pdbe.org/4ax8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ax8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ax8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/WBDD_ECOLX WBDD_ECOLX] Regulates the length of the LPS O-antigen polysaccharide chain. Stops the polymerization of the chain by phosphorylating and then methylating the phosphate on the terminal sugar. This terminal modification is essential for export of the O-antigen across the inner membrane. WbdD is also required for correct localization of the WbdA mannosyltransferase.<ref>PMID:15184370</ref> <ref>PMID:19734145</ref> <ref>PMID:21990359</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hagelueken, G]]	+	[[Category: Hagelueken G]]
-	[[Category: Huang, H]]	+	[[Category: Huang H]]
-	[[Category: Naismith~~, J H]]~~	+	[[Category: Naismith JH]]
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:49, 25 August 2022

OCA — Thu, 25 Aug 2022 05:49:23 GMT

←Older revision		Revision as of 05:49, 25 August 2022
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	<StructureSection load='4ax8' size='340' side='right'caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4ax8' size='340' side='right'caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [~~http~~://pdbe.org/4ax8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ax8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ax8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [https://pdbe.org/4ax8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ax8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ax8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Hagelueken, G]]		[[Category: Hagelueken, G]]
	[[Category: Huang, H]]		[[Category: Huang, H]]

OCA at 09:43, 6 March 2019

OCA — Wed, 06 Mar 2019 09:43:32 GMT

←Older revision		Revision as of 09:43, 6 March 2019
Line 1:		Line 1:

	==Medium resolution structure of the bifunctional kinase- methyltransferase WbdD==		==Medium resolution structure of the bifunctional kinase- methyltransferase WbdD==
-	<StructureSection load='4ax8' size='340' side='right' caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4ax8' size='340' side='right'caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>

OCA at 20:27, 11 August 2016

OCA — Thu, 11 Aug 2016 20:27:10 GMT

←Older revision		Revision as of 20:27, 11 August 2016
Line 1:		Line 1:
		+
	==Medium resolution structure of the bifunctional kinase- methyltransferase WbdD==		==Medium resolution structure of the bifunctional kinase- methyltransferase WbdD==
	<StructureSection load='4ax8' size='340' side='right' caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4ax8' size='340' side='right' caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ax8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [http://pdbe.org/4ax8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ax8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ax8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ax8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Hagelueken, G]]		[[Category: Hagelueken, G]]
	[[Category: Huang, H]]		[[Category: Huang, H]]

OCA at 13:16, 5 January 2015

OCA — Mon, 05 Jan 2015 13:16:25 GMT

←Older revision		Revision as of 13:16, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ax8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ax8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Hagelueken, G.]]	+	[[Category: Hagelueken, G]]
-	[[Category: Huang, H.]]	+	[[Category: Huang, H]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:50, 9 July 2014

OCA — Wed, 09 Jul 2014 07:50:52 GMT

←Older revision		Revision as of 07:50, 9 July 2014
Line 1:		Line 1:
-	[[~~Image:~~4ax8.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Medium resolution structure of the bifunctional kinase- methyltransferase WbdD==
		+	<StructureSection load='4ax8' size='340' side='right' caption='[[4ax8]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AX8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azs\|4azs]], [[4azt\|4azt]], [[4azv\|4azv]], [[4azw\|4azw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ax8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ax8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ax8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ax8 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	WbdD is a bifunctional kinase/methyltransferase that is responsible for regulation of lipopolysaccharide O antigen polysaccharide chain length in Escherichia coli serotype O9a. Solving the crystal structure of this protein proved to be a challenge because the available crystals belonging to space group I23 only diffracted to low resolution (>95% of the crystals diffracted to resolution lower than 4 A and most only to 8 A) and were non-isomorphous, with changes in unit-cell dimensions of greater than 10%. Data from a serendipitously found single native crystal that diffracted to 3.0 A resolution were non-isomorphous with a lower (3.5 A) resolution selenomethionine data set. Here, a strategy for improving poor (3.5 A resolution) initial phases by density modification and cross-crystal averaging with an additional 4.2 A resolution data set to build a crude model of WbdD is desribed. Using this crude model as a mask to cut out the 3.5 A resolution electron density yielded a successful molecular-replacement solution of the 3.0 A resolution data set. The resulting map was used to build a complete model of WbdD. The hydration status of individual crystals appears to underpin the variable diffraction quality of WbdD crystals. After the initial structure had been solved, methods to control the hydration status of WbdD were developed and it was thus possible to routinely obtain high-resolution diffraction (to better than 2.5 A resolution). This novel and facile crystal-dehydration protocol may be useful for similar challenging situations.

-	~~{{STRUCTURE_4ax8\| PDB=4ax8 \| SCENE= }}~~	+	Crystallization, dehydration and experimental phasing of WbdD, a bifunctional kinase and methyltransferase from Escherichia coli O9a.,Hagelueken G, Huang H, Harlos K, Clarke BR, Whitfield C, Naismith JH Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1371-9. Epub 2012 Sep 18. PMID:22993091<ref>PMID:22993091</ref>

-	~~===Medium resolution structure~~ of the ~~bifunctional kinase- methyltransferase WbdD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22993091}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4ax8]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA].~~	+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Hagelueken, G.]]		[[Category: Hagelueken, G.]]

OCA at 06:17, 3 October 2012

OCA — Wed, 03 Oct 2012 06:17:29 GMT

←Older revision		Revision as of 06:17, 3 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4ax8.jpg\|left\|200px]]

-	~~The entry~~ 4ax8 ~~is ON HOLD~~	+	{{STRUCTURE_4ax8\| PDB=4ax8 \| SCENE= }}

-	~~Authors: Hagelueken, G., Huang, H., Naismith, J.H.~~	+	===Medium resolution structure of the bifunctional kinase- methyltransferase WbdD===

-	~~Description: Medium resolution~~ structure ~~of the bifunctional kinase~~-~~methyltransferase WbdD~~	+	{{ABSTRACT_PUBMED_22993091}}
		+
		+	==About this Structure==
		+	[[4ax8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AX8 OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Hagelueken, G.]]
		+	[[Category: Huang, H.]]
		+	[[Category: Naismith, J H.]]
		+	[[Category: Kinase]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Transferase]]

OCA: Protected "4ax8" [edit=sysop:move=sysop]

OCA — Wed, 20 Jun 2012 07:16:49 GMT

Protected "4ax8" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:16, 20 June 2012

OCA: New page: '''Unreleased structure''' The entry 4ax8 is ON HOLD Authors: Hagelueken, G., Huang, H., Naismith, J.H. Description: Medium resolution structure of the bifunctional kinase-methyltransf...

OCA — Wed, 20 Jun 2012 07:16:48 GMT

New page: '''Unreleased structure''' The entry 4ax8 is ON HOLD Authors: Hagelueken, G., Huang, H., Naismith, J.H. Description: Medium resolution structure of the bifunctional kinase-methyltransf...

New page

'''Unreleased structure'''

The entry 4ax8 is ON HOLD

Authors: Hagelueken, G., Huang, H., Naismith, J.H.

Description: Medium resolution structure of the bifunctional kinase-methyltransferase WbdD