4b0j - Revision history

OCA at 11:40, 20 December 2023

OCA — Wed, 20 Dec 2023 11:40:26 GMT

←Older revision		Revision as of 11:40, 20 December 2023
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	<StructureSection load='4b0j' size='340' side='right'caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4b0j' size='340' side='right'caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B0J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=3MQ:(5-THIOPHEN~~-2~~-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/3~~-~~hydroxyacyl~~-~~[acyl~~-~~carrier~~-~~protein]_dehydratase~~ 3-~~hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [https://pdbe.org/4b0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [https://www.ebi.ac.uk/pdbsum/4b0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b0j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [https://pdbe.org/4b0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [https://www.ebi.ac.uk/pdbsum/4b0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).	+	[https://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Duthie~~, F G~~]]	+	[[Category: Duthie FG]]
-	[[Category: McMahon~~, S A~~]]	+	[[Category: McMahon SA]]
-	[[Category: Moynie, L]]	+	[[Category: Moynie L]]
-	[[Category: Naismith~~, J H]]~~	+	[[Category: Naismith JH]]
-	~~[[Category: Bacterial virulence]]~~	+
-	~~[[Category: Drug discovery]]~~	+
-	~~[[Category: Fatty acid biosynthesis]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 05:52, 25 August 2022

OCA — Thu, 25 Aug 2022 05:52:56 GMT

←Older revision		Revision as of 05:52, 25 August 2022
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	<StructureSection load='4b0j' size='340' side='right'caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4b0j' size='340' side='right'caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B0J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-hydroxyacyl-[acyl-carrier-protein]_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-hydroxyacyl-[acyl-carrier-protein]_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [~~http~~://pdbe.org/4b0j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b0j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b0j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [https://pdbe.org/4b0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [https://www.ebi.ac.uk/pdbsum/4b0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).	+	[[https://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:55, 18 March 2020

OCA — Wed, 18 Mar 2020 10:55:34 GMT

←Older revision		Revision as of 10:55, 18 March 2020
Line 1:		Line 1:

	==Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol==		==Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol==
-	<StructureSection load='4b0j' size='340' side='right' caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4b0j' size='340' side='right'caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B0J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B0J FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Beta-hydroxyacyl-acyl carrier protein dehydratase\|Beta-hydroxyacyl-acyl carrier protein dehydratase]]	+	*[[Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures\|Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Duthie, F G]]		[[Category: Duthie, F G]]

OCA at 06:43, 5 August 2016

OCA — Fri, 05 Aug 2016 06:43:46 GMT

←Older revision		Revision as of 06:43, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol==		==Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol==
	<StructureSection load='4b0j' size='340' side='right' caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4b0j' size='340' side='right' caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B0J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-hydroxyacyl-[acyl-carrier-protein]_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-hydroxyacyl-[acyl-carrier-protein]_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [http://www.ebi.ac.uk/pdbsum/4b0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [http://pdbe.org/4b0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [http://www.ebi.ac.uk/pdbsum/4b0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b0j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas aeruginosa pao1~~]]	+	[[Category: Pseae]]
	[[Category: Duthie, F G]]		[[Category: Duthie, F G]]
	[[Category: McMahon, S A]]		[[Category: McMahon, S A]]

OCA at 16:37, 25 December 2014

OCA — Thu, 25 Dec 2014 16:37:01 GMT

←Older revision		Revision as of 16:37, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [http://www.ebi.ac.uk/pdbsum/4b0j PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [http://www.ebi.ac.uk/pdbsum/4b0j PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:54, 21 December 2014

OCA — Sun, 21 Dec 2014 08:54:14 GMT

←Older revision		Revision as of 08:54, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}~~	+	==Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol==
-	===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===	+	<StructureSection load='4b0j' size='340' side='right' caption='[[4b0j]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23174186}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B0J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MQ:(5-THIOPHEN-2-YLISOXAZOL-3-YL)METHANOL'>3MQ</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b0b\|4b0b]], [[4b0c\|4b0c]], [[4b0i\|4b0i]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-hydroxyacyl-[acyl-carrier-protein]_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.59 4.2.1.59] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b0j RCSB], [http://www.ebi.ac.uk/pdbsum/4b0j PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fatty acid biosynthesis is an essential component of metabolism in both eukaryotes and prokaryotes. The fatty acid biosynthetic pathway of Gram-negative bacteria is an established therapeutic target. Two homologous enzymes FabA and FabZ catalyze a key step in fatty acid biosynthesis; both dehydrate hydroxyacyl fatty acids that are coupled via a phosphopantetheine to an acyl carrier protein (ACP). The resulting trans-2-enoyl-ACP is further polymerized in a processive manner. FabA, however, carries out a second reaction involving isomerization of trans-2-enoyl fatty acid to cis-3-enoyl fatty acid. We have solved the structure of Pseudomonas aeruginosa FabA with a substrate allowing detailed molecular insight into the interactions of the active site. This has allowed a detailed examination of the factors governing the second catalytic step. We have also determined the structure of FabA in complex with small molecules (so-called fragments). These small molecules occupy distinct regions of the active site and form the basis for a rational inhibitor design program.

-	~~==Function==~~	+	Structural Insights into the Mechanism and Inhibition of the beta-Hydroxydecanoyl-Acyl Carrier Protein Dehydratase from Pseudomonasaeruginosa.,Moynie L, Leckie SM, McMahon SA, Duthie FG, Koehnke A, Taylor JW, Alphey MS, Brenk R, Smith AD, Naismith JH J Mol Biol. 2013 Jan 23;425(2):365-77. doi: 10.1016/j.jmb.2012.11.017. Epub 2012 , Nov 19. PMID:23174186<ref>PMID:23174186</ref>
-	~~[[http://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for~~ the ~~introduction~~ of ~~cis unsaturation into fatty acids. Catalyzes~~ the ~~dehydration of (3R)~~-~~3-hydroxydecanoyl-ACP to E~~-(2)-~~decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP~~. ~~Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16~~:0, ~~being most active on intermediate chain length (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4b0j]] is~~ a ~~20 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023174186</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Beta-hydroxyacyl-acyl carrier protein dehydratase\|Beta-hydroxyacyl-acyl carrier protein dehydratase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]
-	[[Category: Duthie, F G.]]	+	[[Category: Duthie, F G]]
-	[[Category: McMahon, S A.]]	+	[[Category: McMahon, S A]]
-	[[Category: Moynie, L.]]	+	[[Category: Moynie, L]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
	[[Category: Bacterial virulence]]		[[Category: Bacterial virulence]]
	[[Category: Drug discovery]]		[[Category: Drug discovery]]
	[[Category: Fatty acid biosynthesis]]		[[Category: Fatty acid biosynthesis]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 08:49, 4 September 2013

OCA — Wed, 04 Sep 2013 08:49:17 GMT

←Older revision		Revision as of 08:49, 4 September 2013
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-	[[Image:4b0j.png\|left\|200px]]
-
	{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}		{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}
-
	===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===		===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===
-
	{{ABSTRACT_PUBMED_23174186}}		{{ABSTRACT_PUBMED_23174186}}
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/FABA_PSEAE FABA_PSEAE]] Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).

	==About this Structure==		==About this Structure==
	[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA].		[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023174186</ref><references group="xtra"/><references/>
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]
	[[Category: Duthie, F G.]]		[[Category: Duthie, F G.]]
-	[[Category: ~~Mcmahon~~, S A.]]	+	[[Category: McMahon, S A.]]
	[[Category: Moynie, L.]]		[[Category: Moynie, L.]]
	[[Category: Naismith, J H.]]		[[Category: Naismith, J H.]]

OCA at 12:28, 30 January 2013

OCA — Wed, 30 Jan 2013 12:28:01 GMT

←Older revision		Revision as of 12:28, 30 January 2013
Line 1:		Line 1:
-	[[Image:4b0j.~~jpg~~\|left\|200px]]	+	[[Image:4b0j.png\|left\|200px]]

	{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}		{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}
Line 5:		Line 5:
	===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===		===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===

		+	{{ABSTRACT_PUBMED_23174186}}

	==About this Structure==		==About this Structure==

OCA at 14:37, 28 November 2012

OCA — Wed, 28 Nov 2012 14:37:14 GMT

←Older revision		Revision as of 14:37, 28 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4b0j.jpg\|left\|200px]]

-	~~The entry~~ 4b0j ~~is ON HOLD~~	+	{{STRUCTURE_4b0j\| PDB=4b0j \| SCENE= }}

-	~~Authors: Moynie, L., McMahon, S.A., Duthie, F.G., Naismith, J.H.~~	+	===Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 5-(2- thienyl)-3-isoxazolyl methanol===

-	~~Description: Crystal~~ Structure ~~of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA)~~ from Pseudomonas aeruginosa ~~in complex with 5~~-~~(2-thienyl)-3-isoxazolyl methanol~~	+
		+	==About this Structure==
		+	[[4b0j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B0J OCA].
		+	[[Category: Pseudomonas aeruginosa pao1]]
		+	[[Category: Duthie, F G.]]
		+	[[Category: Mcmahon, S A.]]
		+	[[Category: Moynie, L.]]
		+	[[Category: Naismith, J H.]]
		+	[[Category: Bacterial virulence]]
		+	[[Category: Drug discovery]]
		+	[[Category: Fatty acid biosynthesis]]
		+	[[Category: Lyase]]

OCA: Protected "4b0j" [edit=sysop:move=sysop]

OCA — Wed, 11 Jul 2012 07:41:30 GMT

Protected "4b0j" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:41, 11 July 2012