4b20 - Revision history

OCA at 10:55, 9 May 2024

OCA — Thu, 09 May 2024 10:55:54 GMT

←Older revision		Revision as of 10:55, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='4b20' size='340' side='right'caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4b20' size='340' side='right'caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B20 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B20 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2w35\|2w35]], [[2w36\|2w36]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [https://pdbe.org/4b20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [https://www.ebi.ac.uk/pdbsum/4b20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b20 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [https://pdbe.org/4b20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [https://www.ebi.ac.uk/pdbsum/4b20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b20 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>	+	[https://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43589]]
-	[[Category: Deoxyribonuclease V]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alseth, I]]	+	[[Category: Thermotoga maritima]]
-	[[Category: Bjoras, M]]	+	[[Category: Alseth I]]
-	[[Category: Dalhus, B]]	+	[[Category: Bjoras M]]
-	[[Category: Forstrom~~, R J~~]]	+	[[Category: Dalhus B]]
-	[[Category: Rosnes, I]]	+	[[Category: Forstrom RJ]]
-	[[Category: Rowe~~, A D]]~~	+	[[Category: Rosnes I]]
-	~~[[Category: Hydrolase~~]]	+	[[Category: Rowe AD]]

OCA at 06:57, 31 August 2022

OCA — Wed, 31 Aug 2022 06:57:04 GMT

←Older revision		Revision as of 06:57, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4b20' size='340' side='right'caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4b20' size='340' side='right'caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B20 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B20 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2w35\|2w35]], [[2w36\|2w36]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2w35\|2w35]], [[2w36\|2w36]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [~~http~~://pdbe.org/4b20 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b20 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b20 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [https://pdbe.org/4b20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [https://www.ebi.ac.uk/pdbsum/4b20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b20 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>	+	[[https://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:12, 13 March 2019

OCA — Wed, 13 Mar 2019 13:12:06 GMT

←Older revision		Revision as of 13:12, 13 March 2019
Line 1:		Line 1:

	==Structural basis of DNA loop recognition by Endonuclease V==		==Structural basis of DNA loop recognition by Endonuclease V==
-	<StructureSection load='4b20' size='340' side='right' caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='4b20' size='340' side='right'caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B20 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B20 FirstGlance]. <br>
Line 29:		Line 29:
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
	[[Category: Deoxyribonuclease V]]		[[Category: Deoxyribonuclease V]]
		+	[[Category: Large Structures]]
	[[Category: Alseth, I]]		[[Category: Alseth, I]]
	[[Category: Bjoras, M]]		[[Category: Bjoras, M]]

OCA at 00:11, 5 August 2016

OCA — Fri, 05 Aug 2016 00:11:51 GMT

←Older revision		Revision as of 00:11, 5 August 2016
Line 1:		Line 1:
		+
	==Structural basis of DNA loop recognition by Endonuclease V==		==Structural basis of DNA loop recognition by Endonuclease V==
	<StructureSection load='4b20' size='340' side='right' caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4b20' size='340' side='right' caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B20 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B20 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2w35\|2w35]], [[2w36\|2w36]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2w35\|2w35]], [[2w36\|2w36]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [http://www.ebi.ac.uk/pdbsum/4b20 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [http://pdbe.org/4b20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [http://www.ebi.ac.uk/pdbsum/4b20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b20 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b20" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43589]]
	[[Category: Deoxyribonuclease V]]		[[Category: Deoxyribonuclease V]]
-	[[Category: Thermotoga maritima]]
	[[Category: Alseth, I]]		[[Category: Alseth, I]]
	[[Category: Bjoras, M]]		[[Category: Bjoras, M]]

OCA at 21:03, 24 December 2014

OCA — Wed, 24 Dec 2014 21:03:57 GMT

←Older revision		Revision as of 21:03, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [http://www.ebi.ac.uk/pdbsum/4b20 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [http://www.ebi.ac.uk/pdbsum/4b20 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:09, 21 December 2014

OCA — Sun, 21 Dec 2014 09:09:34 GMT

←Older revision		Revision as of 09:09, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4b20\| PDB=4b20 \| SCENE= }}~~	+	==Structural basis of DNA loop recognition by Endonuclease V==
-	===Structural basis of DNA loop recognition by Endonuclease V===	+	<StructureSection load='4b20' size='340' side='right' caption='[[4b20]], [[Resolution\|resolution]] 2.75Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23313664}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B20 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2w35\|2w35]], [[2w36\|2w36]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_V Deoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.7 3.1.21.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b20 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b20 RCSB], [http://www.ebi.ac.uk/pdbsum/4b20 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The DNA repair enzyme endonuclease V (EndoV) recognizes and cleaves DNA at deaminated adenine lesions (hypoxanthine). In addition, EndoV cleaves DNA containing various helical distortions such as loops, hairpins, and flaps. To understand the molecular basis of EndoV's ability to recognize and incise DNA structures with helical distortions, we solved the crystal structure of Thermotoga maritima EndoV in complex with DNA containing a one-nucleotide loop. The structure shows that a strand-separating wedge is crucial for DNA loop recognition, with DNA strands separated precisely at the helical distortion. The additional nucleotide forming the loop rests on the surface of the wedge, while the normal adenine opposite the loop is flipped into a base recognition pocket. Our data show a different principle for DNA loop recognition and cleavage by EndoV, in which a coordinated action of a DNA-intercalating wedge and a base pocket accommodating a flipped normal base facilitate strand incision.

-	~~==Function==~~	+	Structural basis of DNA loop recognition by endonuclease V.,Rosnes I, Rowe AD, Vik ES, Forstrom RJ, Alseth I, Bjoras M, Dalhus B Structure. 2013 Feb 5;21(2):257-65. doi: 10.1016/j.str.2012.12.007. Epub 2013 Jan, 11. PMID:23313664<ref>PMID:23313664</ref>
-	~~[[http://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked~~ DNA. ~~Acts in DNA repair. In vitro~~, ~~can also cleave single-stranded substrates with inosine~~, ~~double-stranded DNA with apurinic sites~~, ~~or DNA sites with uracil or a mismatched base. When present in molar excess~~, ~~two protein molecules can bind to the same DNA substrate and effect cleavage of both strands~~ (~~in vitro~~).<ref>PMID:~~12081482~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4b20]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	<references ~~group="xtra"~~/><~~references~~/>	+	*[[Endonuclease\|Endonuclease]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Deoxyribonuclease V]]		[[Category: Deoxyribonuclease V]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-	[[Category: Alseth, I.]]	+	[[Category: Alseth, I]]
-	[[Category: Bjoras, M.]]	+	[[Category: Bjoras, M]]
-	[[Category: Dalhus, B.]]	+	[[Category: Dalhus, B]]
-	[[Category: Forstrom, R J.]]	+	[[Category: Forstrom, R J]]
-	[[Category: Rosnes, I.]]	+	[[Category: Rosnes, I]]
-	[[Category: Rowe, A D.]]	+	[[Category: Rowe, A D]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 20:22, 17 April 2013

OCA — Wed, 17 Apr 2013 20:22:29 GMT

←Older revision		Revision as of 20:22, 17 April 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4b20\| PDB=4b20 \| SCENE= }}
		+	===Structural basis of DNA loop recognition by Endonuclease V===
		+	{{ABSTRACT_PUBMED_23313664}}

-	~~The entry 4b20 is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/NFI_THEMA NFI_THEMA]] Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).<ref>PMID:12081482</ref>

-	~~Authors~~: ~~Rosnes, I~~.~~, Rowe, A~~.D.~~, Forstrom, R.J~~.~~, Alseth, I~~.~~, Bjoras, M~~.~~, Dalhus, B~~.	+	==About this Structure==
		+	[[4b20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B20 OCA].

-	~~Description~~: ~~Structural basis of DNA loop recognition by Endonuclease~~ V	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Deoxyribonuclease V]]
		+	[[Category: Thermotoga maritima]]
		+	[[Category: Alseth, I.]]
		+	[[Category: Bjoras, M.]]
		+	[[Category: Dalhus, B.]]
		+	[[Category: Forstrom, R J.]]
		+	[[Category: Rosnes, I.]]
		+	[[Category: Rowe, A D.]]
		+	[[Category: Hydrolase]]

OCA at 13:53, 28 November 2012

OCA — Wed, 28 Nov 2012 13:53:12 GMT

←Older revision		Revision as of 13:53, 28 November 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4b20 is ON HOLD	+	The entry 4b20 is ON HOLD until Paper Publication

	Authors: Rosnes, I., Rowe, A.D., Forstrom, R.J., Alseth, I., Bjoras, M., Dalhus, B.		Authors: Rosnes, I., Rowe, A.D., Forstrom, R.J., Alseth, I., Bjoras, M., Dalhus, B.

	Description: Structural basis of DNA loop recognition by Endonuclease V		Description: Structural basis of DNA loop recognition by Endonuclease V

OCA: Protected "4b20" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2012 04:55:22 GMT

Protected "4b20" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 18 July 2012

OCA: New page: '''Unreleased structure''' The entry 4b20 is ON HOLD Authors: Rosnes, I., Rowe, A.D., Forstrom, R.J., Alseth, I., Bjoras, M., Dalhus, B. Description: Structural basis of DNA loop recog...

OCA — Wed, 18 Jul 2012 04:55:21 GMT

New page: '''Unreleased structure''' The entry 4b20 is ON HOLD Authors: Rosnes, I., Rowe, A.D., Forstrom, R.J., Alseth, I., Bjoras, M., Dalhus, B. Description: Structural basis of DNA loop recog...

New page

'''Unreleased structure'''

The entry 4b20 is ON HOLD

Authors: Rosnes, I., Rowe, A.D., Forstrom, R.J., Alseth, I., Bjoras, M., Dalhus, B.

Description: Structural basis of DNA loop recognition by Endonuclease V