4b3v - Revision history

OCA at 11:42, 20 December 2023

OCA — Wed, 20 Dec 2023 11:42:42 GMT

←Older revision		Revision as of 11:42, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubella_virus Rubella virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [https://pdbe.org/4b3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [https://www.ebi.ac.uk/pdbsum/4b3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [https://pdbe.org/4b3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [https://www.ebi.ac.uk/pdbsum/4b3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM]] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.	+	[https://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~DuBois, R M~~]]	+	[[Category: Rubella virus]]
-	[[Category: ~~Rey, F A~~]]	+	[[Category: DuBois RM]]
-	[[Category: ~~Tortorici, M A~~]]	+	[[Category: Rey FA]]
-	[[Category: ~~Vaney, M C~~]]	+	[[Category: Tortorici MA]]
-	[[Category: ~~Envelope glycoprotein]]~~	+	[[Category: Vaney MC]]
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 06:59, 31 August 2022

OCA — Wed, 31 Aug 2022 06:59:00 GMT

←Older revision		Revision as of 06:59, 31 August 2022
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	<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Rubv Rubv]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B3V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [~~http~~://pdbe.org/4b3v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b3v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [https://pdbe.org/4b3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [https://www.ebi.ac.uk/pdbsum/4b3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM]] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.	+	[[https://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM]] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rubv]]
	[[Category: DuBois, R M]]		[[Category: DuBois, R M]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 06:16, 16 October 2019

OCA — Wed, 16 Oct 2019 06:16:26 GMT

←Older revision		Revision as of 06:16, 16 October 2019
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	<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rubv Rubv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Rubv]]
	[[Category: DuBois, R M]]		[[Category: DuBois, R M]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 16:49, 28 August 2019

OCA — Wed, 28 Aug 2019 16:49:03 GMT

←Older revision		Revision as of 16:49, 28 August 2019
Line 1:		Line 1:

	==Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate==		==Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate==
-	<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>	+	<StructureSection load='4b3v' size='340' side='right'caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: DuBois, R M]]		[[Category: DuBois, R M]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 11:36, 3 August 2017

OCA — Thu, 03 Aug 2017 11:36:17 GMT

←Older revision		Revision as of 11:36, 3 August 2017
Line 3:		Line 3:
	<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Rubv Rubv]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Rubv]]
	[[Category: DuBois, R M]]		[[Category: DuBois, R M]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 17:01, 2 January 2017

OCA — Mon, 02 Jan 2017 17:01:14 GMT

←Older revision		Revision as of 17:01, 2 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate==		==Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate==
	<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [http://www.ebi.ac.uk/pdbsum/4b3v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [http://pdbe.org/4b3v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [http://www.ebi.ac.uk/pdbsum/4b3v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b3v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:59, 18 March 2015

OCA — Wed, 18 Mar 2015 11:59:19 GMT

←Older revision		Revision as of 11:59, 18 March 2015
Line 2:		Line 2:
	<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rubella_virus Rubella virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rubv Rubv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [http://www.ebi.ac.uk/pdbsum/4b3v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [http://www.ebi.ac.uk/pdbsum/4b3v PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM]] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rubella virus~~]]	+	[[Category: Rubv]]
-	[[Category: ~~Dubois~~, R M]]	+	[[Category: DuBois, R M]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]
	[[Category: Tortorici, M A]]		[[Category: Tortorici, M A]]

OCA at 17:36, 9 December 2014

OCA — Tue, 09 Dec 2014 17:36:21 GMT

←Older revision		Revision as of 17:36, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4b3v.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate==
		+	<StructureSection load='4b3v' size='340' side='right' caption='[[4b3v]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b3v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rubella_virus Rubella virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4adg\|4adg]], [[4adi\|4adi]], [[4adj\|4adj]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3v RCSB], [http://www.ebi.ac.uk/pdbsum/4b3v PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Little is known about the three-dimensional organization of rubella virus, which causes a relatively mild measles-like disease in children but leads to serious congenital health problems when contracted in utero. Although rubella virus belongs to the same family as the mosquito-borne alphaviruses, in many respects it is more similar to other aerosol-transmitted human viruses such as the agents of measles and mumps. Although the use of the triple MMR (measles, mumps and rubella) live vaccine has limited its incidence in western countries, congenital rubella syndrome remains an important health problem in the developing world. Here we report the 1.8 A resolution crystal structure of envelope glycoprotein E1, the main antigen and sole target of neutralizing antibodies against rubella virus. E1 is the main player during entry into target cells owing to its receptor-binding and membrane-fusion functions. The structure reveals the epitope and the neutralization mechanism of an important category of protecting antibodies against rubella infection. It also shows that rubella virus E1 is a class II fusion protein, which had hitherto only been structurally characterized for the arthropod-borne alphaviruses and flaviviruses. In addition, rubella virus E1 has an extensive membrane-fusion surface that includes a metal site, reminiscent of the T-cell immunoglobulin and mucin family of cellular proteins that bind phosphatidylserine lipids at the plasma membrane of cells undergoing apoptosis. Such features have not been seen in any fusion protein crystallized so far. Structural comparisons show that the class II fusion proteins from alphaviruses and flaviviruses, despite belonging to different virus families, are closer to each other than they are to rubella virus E1. This suggests that the constraints on arboviruses imposed by alternating cycles between vertebrates and arthropods resulted in more conservative evolution. By contrast, in the absence of this constraint, the strictly human rubella virus seems to have drifted considerably into a unique niche as sole member of the Rubivirus genus.

-	~~{{STRUCTURE_4b3v\| PDB=4b3v \| SCENE= }}~~	+	Functional and evolutionary insight from the crystal structure of rubella virus protein E1.,Dubois RM, Vaney MC, Tortorici MA, Kurdi RA, Barba-Spaeth G, Krey T, Rey FA Nature. 2013 Jan 6. doi: 10.1038/nature11741. PMID:23292515<ref>PMID:23292515</ref>

-	~~===Crystal structure~~ of the ~~Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23292515}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4b3v]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rubella_virus Rubella virus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014737160<~~/~~ref~~><~~ref group="xtra">PMID:016407067</ref><ref group="xtra">PMID:014963486</ref><references group="xtra"~~/>	+
	[[Category: Rubella virus]]		[[Category: Rubella virus]]
-	[[Category: Dubois, R M.]]	+	[[Category: Dubois, R M]]
-	[[Category: Rey, F A.]]	+	[[Category: Rey, F A]]
-	[[Category: Tortorici, M A.]]	+	[[Category: Tortorici, M A]]
-	[[Category: Vaney, M C.]]	+	[[Category: Vaney, M C]]
	[[Category: Envelope glycoprotein]]		[[Category: Envelope glycoprotein]]
	[[Category: Membrane fusion]]		[[Category: Membrane fusion]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 11:42, 23 January 2013

OCA — Wed, 23 Jan 2013 11:42:45 GMT

←Older revision		Revision as of 11:42, 23 January 2013
Line 1:		Line 1:
-	[[Image:4b3v.~~jpg~~\|left\|200px]]	+	[[Image:4b3v.png\|left\|200px]]

	{{STRUCTURE_4b3v\| PDB=4b3v \| SCENE= }}		{{STRUCTURE_4b3v\| PDB=4b3v \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate===		===Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate===

-	{{~~ABSTRACT_PUBMED_11301009~~}}	+	{{ABSTRACT_PUBMED_23292515}}

	==About this Structure==		==About this Structure==
Line 11:		Line 11:

	==Reference==		==Reference==
-	~~<ref group="xtra">PMID:011301009</ref>~~<ref group="xtra">PMID:014737160</ref><ref group="xtra">PMID:016407067</ref><ref group="xtra">PMID:014963486</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014737160</ref><ref group="xtra">PMID:016407067</ref><ref group="xtra">PMID:014963486</ref><references group="xtra"/>
	[[Category: Rubella virus]]		[[Category: Rubella virus]]
	[[Category: Dubois, R M.]]		[[Category: Dubois, R M.]]

OCA at 08:22, 9 January 2013

OCA — Wed, 09 Jan 2013 08:22:23 GMT

←Older revision		Revision as of 08:22, 9 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4b3v.jpg\|left\|200px]]

-	~~The entry~~ 4b3v ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4b3v\| PDB=4b3v \| SCENE= }}

-	~~Authors: Vaney, M.C., DuBois, R.M., Tortorici, M.A., Rey, F.A.~~	+	===Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 20mM of Calcium Acetate===

-	~~Description: Crystal~~ structure ~~of the~~ Rubella virus ~~glycoprotein E1 in its post~~-fusion ~~form crystallized in presence of 20mM of Calcium Acetate~~	+	{{ABSTRACT_PUBMED_11301009}}
		+
		+	==About this Structure==
		+	[[4b3v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rubella_virus Rubella virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3V OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:011301009</ref><ref group="xtra">PMID:014737160</ref><ref group="xtra">PMID:016407067</ref><ref group="xtra">PMID:014963486</ref><references group="xtra"/>
		+	[[Category: Rubella virus]]
		+	[[Category: Dubois, R M.]]
		+	[[Category: Rey, F A.]]
		+	[[Category: Tortorici, M A.]]
		+	[[Category: Vaney, M C.]]
		+	[[Category: Envelope glycoprotein]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Viral protein]]