4b9i - Revision history

OCA at 10:58, 9 May 2024

OCA — Thu, 09 May 2024 10:58:31 GMT

←Older revision		Revision as of 10:58, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='4b9i' size='340' side='right'caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4b9i' size='340' side='right'caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B9I FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[4b9g\|4b9g]]~~, [[~~4b9j~~\|~~4b9j~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [https://pdbe.org/4b9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [https://www.ebi.ac.uk/pdbsum/4b9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [https://pdbe.org/4b9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [https://www.ebi.ac.uk/pdbsum/4b9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.	+	[https://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Knight~~, S D~~]]	+	[[Category: Knight SD]]
-	[[Category: Rahman~~, M M~~]]	+	[[Category: Rahman MM]]
-	[[Category: Roy~~, S P~~]]	+	[[Category: Roy SP]]
-	[[Category: Tuittila, M]]	+	[[Category: Tuittila M]]
-	[[Category: Yu~~, X D~~]]	+	[[Category: Yu XD]]
-	[[Category: Zavialov~~, A V]]~~	+	[[Category: Zavialov AV]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Diarrheal disease]]~~	+
-	~~[[Category: Fimbriae]]~~	+
-	~~[[Category: Fusion protein~~]]	+

OCA at 07:04, 31 August 2022

OCA — Wed, 31 Aug 2022 07:04:38 GMT

←Older revision		Revision as of 07:04, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4b9i' size='340' side='right'caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4b9i' size='340' side='right'caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B9I FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b9g\|4b9g]], [[4b9j\|4b9j]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4b9g\|4b9g]], [[4b9j\|4b9j]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [~~http~~://pdbe.org/4b9i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b9i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [https://pdbe.org/4b9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [https://www.ebi.ac.uk/pdbsum/4b9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.	+	[[https://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Pilin\|Pilin]]	+	*[[Pilin 3D structures\|Pilin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:16, 13 March 2019

OCA — Wed, 13 Mar 2019 13:16:53 GMT

←Older revision		Revision as of 13:16, 13 March 2019
Line 1:		Line 1:

	==Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6==		==Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6==
-	<StructureSection load='4b9i' size='340' side='right' caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='4b9i' size='340' side='right'caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B9I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B9I FirstGlance]. <br>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Knight, S D]]		[[Category: Knight, S D]]
	[[Category: Rahman, M M]]		[[Category: Rahman, M M]]

OCA at 21:37, 4 August 2016

OCA — Thu, 04 Aug 2016 21:37:58 GMT

←Older revision		Revision as of 21:37, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6==		==Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6==
	<StructureSection load='4b9i' size='340' side='right' caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4b9i' size='340' side='right' caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B9I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b9g\|4b9g]], [[4b9j\|4b9j]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b9g\|4b9g]], [[4b9j\|4b9j]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [http://www.ebi.ac.uk/pdbsum/4b9i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [http://pdbe.org/4b9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [http://www.ebi.ac.uk/pdbsum/4b9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b9i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pilin\|Pilin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Knight, S D]]		[[Category: Knight, S D]]
	[[Category: Rahman, M M]]		[[Category: Rahman, M M]]

OCA at 19:53, 24 December 2014

OCA — Wed, 24 Dec 2014 19:53:51 GMT

←Older revision		Revision as of 19:53, 24 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4b9i.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6==
		+	<StructureSection load='4b9i' size='340' side='right' caption='[[4b9i]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B9I FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b9g\|4b9g]], [[4b9j\|4b9j]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b9i RCSB], [http://www.ebi.ac.uk/pdbsum/4b9i PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coli surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone usher (CU) pathway. Here, we present an atomic resolution model for the structure of CS6 based on the results of X-ray crystallographic, spectroscopic and biochemical studies, and suggest a mechanism for CS6-mediated adhesion. We show that the CssA and CssB subunits are assembled alternately in linear fibres by the principle of donor strand complementation. This type of fibre assembly is novel for CU assembled adhesins. We also show that both subunits in the fibre bind to receptors on epithelial cells, and that CssB, but not CssA, specifically recognizes the extracellular matrix protein fibronectin. Taken together, structural and functional results suggest that CS6 is an adhesive organelle of a novel type, a hetero-polyadhesin that is capable of polyvalent attachment to different receptors.

-	~~{{STRUCTURE_4b9i\| PDB=4b9i \| SCENE= }}~~	+	Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.,Roy SP, Rahman MM, Yu XD, Tuittila M, Knight SD, Zavialov AV Mol Microbiol. 2012 Oct 10. doi: 10.1111/mmi.12044. PMID:23046340<ref>PMID:23046340</ref>

-	~~===Structure~~ of ~~CssA subunit complemented with donor strand from CssB subunit~~ of ~~enterotoxigenic Escherichia coli colonization factor CS6===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23046340}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4b9i]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA].~~	+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Knight, S D.]]	+	[[Category: Knight, S D]]
-	[[Category: Rahman, M M.]]	+	[[Category: Rahman, M M]]
-	[[Category: Roy, S P.]]	+	[[Category: Roy, S P]]
-	[[Category: Tuittila, M.]]	+	[[Category: Tuittila, M]]
-	[[Category: Yu, X D.]]	+	[[Category: Yu, X D]]
-	[[Category: Zavialov, A V.]]	+	[[Category: Zavialov, A V]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Diarrheal disease]]		[[Category: Diarrheal disease]]
	[[Category: Fimbriae]]		[[Category: Fimbriae]]
	[[Category: Fusion protein]]		[[Category: Fusion protein]]

OCA at 21:31, 12 December 2012

OCA — Wed, 12 Dec 2012 21:31:26 GMT

←Older revision		Revision as of 21:31, 12 December 2012
Line 1:		Line 1:
-	[[Image:4b9i.~~jpg~~\|left\|200px]]	+	[[Image:4b9i.png\|left\|200px]]

	{{STRUCTURE_4b9i\| PDB=4b9i \| SCENE= }}		{{STRUCTURE_4b9i\| PDB=4b9i \| SCENE= }}
Line 5:		Line 5:
	===Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6===		===Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6===

		+	{{ABSTRACT_PUBMED_23046340}}

	==About this Structure==		==About this Structure==

OCA at 13:45, 8 November 2012

OCA — Thu, 08 Nov 2012 13:45:48 GMT

←Older revision		Revision as of 13:45, 8 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4b9i.jpg\|left\|200px]]

-	~~The entry~~ 4b9i ~~is ON HOLD~~	+	{{STRUCTURE_4b9i\| PDB=4b9i \| SCENE= }}

-	~~Authors: Roy, S., Rahman, M., Yu, X.D., Tuittila, M., Knight, S.D., Zavialov, A.V.~~	+	===Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6===

-	~~Description:~~ Structure ~~of CssA subunit complemented~~ with ~~donor strand~~ from ~~CssB subunit of enterotoxigenic~~ Escherichia coli ~~colonization factor CS6~~	+
		+	==About this Structure==
		+	[[4b9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9I OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Knight, S D.]]
		+	[[Category: Rahman, M M.]]
		+	[[Category: Roy, S P.]]
		+	[[Category: Tuittila, M.]]
		+	[[Category: Yu, X D.]]
		+	[[Category: Zavialov, A V.]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Diarrheal disease]]
		+	[[Category: Fimbriae]]
		+	[[Category: Fusion protein]]

OCA: Protected "4b9i" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2012 04:42:57 GMT

Protected "4b9i" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:42, 12 September 2012

OCA: New page: '''Unreleased structure''' The entry 4b9i is ON HOLD Authors: Roy, S., Rahman, M., Yu, X.D., Tuittila, M., Knight, S.D., Zavialov, A.V. Description: Structure of CssA subunit complemen...

OCA — Wed, 12 Sep 2012 04:42:56 GMT

New page: '''Unreleased structure''' The entry 4b9i is ON HOLD Authors: Roy, S., Rahman, M., Yu, X.D., Tuittila, M., Knight, S.D., Zavialov, A.V. Description: Structure of CssA subunit complemen...

New page

'''Unreleased structure'''

The entry 4b9i is ON HOLD

Authors: Roy, S., Rahman, M., Yu, X.D., Tuittila, M., Knight, S.D., Zavialov, A.V.

Description: Structure of CssA subunit complemented with donor strand from CssB subunit of enterotoxigenic Escherichia coli colonization factor CS6