4bea - Revision history

OCA at 07:47, 7 February 2024

OCA — Wed, 07 Feb 2024 07:47:23 GMT

←Older revision		Revision as of 07:47, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.57Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [https://pdbe.org/4bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [https://www.ebi.ac.uk/pdbsum/4bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [https://pdbe.org/4bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [https://www.ebi.ac.uk/pdbsum/4bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>		[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	eIF4E is frequently over-expressed in different cancers and causes increased translation of oncogenic proteins via deregulated cap-dependent translation. Inhibitors of the eIF4E:eIF4G interactions represent an approach that would normalize cap-dependent translation. Stapled peptides represent an emerging class of therapeutics that can target protein: protein interactions. We present here molecular dynamics simulations for a set of rationally designed stapled peptides in solution and in complex with eIF4E, supported with biophysical and crystallographic data. Clustering of the simulated structures revealed the favoured conformational states of the stapled peptides in their bound or free forms in solution. Identifying these populations has allowed us to design peptides with improved affinities by introducing mutations into the peptide sequence to alter their conformational distributions. These studies emphasise the effects that engineered mutations have on the conformations of free and bound peptides, and illustrate that both states must be considered in efforts to attain high affinity binding.
-
-	Rational Optimization of Conformational Effects Induced By Hydrocarbon Staples in Peptides and their Binding Interfaces.,Lama D, Quah ST, Verma CS, Lakshminarayanan R, Beuerman RW, Lane DP, Brown CJ Sci Rep. 2013 Dec 13;3:3451. doi: 10.1038/srep03451. PMID:24336354<ref>PMID:24336354</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4bea" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:56, 15 March 2023

OCA — Wed, 15 Mar 2023 09:56:09 GMT

←Older revision		Revision as of 09:56, 15 March 2023
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	<StructureSection load='4bea' size='340' side='right'caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>		<StructureSection load='4bea' size='340' side='right'caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=~~NME~~:~~METHYLAMINE~~'>~~NME~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [https://pdbe.org/4bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [https://www.ebi.ac.uk/pdbsum/4bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [https://pdbe.org/4bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [https://www.ebi.ac.uk/pdbsum/4bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>	+	[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Brown, C J~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Lama, D~~]]	+	[[Category: Brown CJ]]
-	[[Category: ~~Lane,~~ D P]]	+	[[Category: Lama D]]
-	[[Category: ~~Quah, S T~~]]	+	[[Category: Lane DP]]
-	[[Category: ~~Verma, C S~~]]	+	[[Category: Quah ST]]
-	[[Category: ~~Translation~~]]	+	[[Category: Verma CS]]

OCA at 07:10, 31 August 2022

OCA — Wed, 31 Aug 2022 07:10:29 GMT

←Older revision		Revision as of 07:10, 31 August 2022
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	<StructureSection load='4bea' size='340' side='right'caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>		<StructureSection load='4bea' size='340' side='right'caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BEA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [~~http~~://pdbe.org/4bea PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bea PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [https://pdbe.org/4bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [https://www.ebi.ac.uk/pdbsum/4bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>	+	[[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:27, 29 January 2020

OCA — Wed, 29 Jan 2020 17:27:06 GMT

←Older revision		Revision as of 17:27, 29 January 2020
Line 1:		Line 1:

	==Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative==		==Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative==
-	<StructureSection load='4bea' size='340' side='right' caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>	+	<StructureSection load='4bea' size='340' side='right'caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]	+	*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Brown, C J]]		[[Category: Brown, C J]]
	[[Category: Lama, D]]		[[Category: Lama, D]]

OCA at 10:50, 4 August 2016

OCA — Thu, 04 Aug 2016 10:50:15 GMT

←Older revision		Revision as of 10:50, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative==		==Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative==
	<StructureSection load='4bea' size='340' side='right' caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>		<StructureSection load='4bea' size='340' side='right' caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [http://www.ebi.ac.uk/pdbsum/4bea PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [http://pdbe.org/4bea PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [http://www.ebi.ac.uk/pdbsum/4bea PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bea" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:32, 25 December 2014

OCA — Thu, 25 Dec 2014 08:32:23 GMT

←Older revision		Revision as of 08:32, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [http://www.ebi.ac.uk/pdbsum/4bea PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [http://www.ebi.ac.uk/pdbsum/4bea PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:36, 21 December 2014

OCA — Sun, 21 Dec 2014 17:36:55 GMT

←Older revision		Revision as of 17:36, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bea\| PDB=4bea \| SCENE= }}~~	+	==Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative==
-	===Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative===	+	<StructureSection load='4bea' size='340' side='right' caption='[[4bea]], [[Resolution\|resolution]] 2.57Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24336354}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEA FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bea RCSB], [http://www.ebi.ac.uk/pdbsum/4bea PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	eIF4E is frequently over-expressed in different cancers and causes increased translation of oncogenic proteins via deregulated cap-dependent translation. Inhibitors of the eIF4E:eIF4G interactions represent an approach that would normalize cap-dependent translation. Stapled peptides represent an emerging class of therapeutics that can target protein: protein interactions. We present here molecular dynamics simulations for a set of rationally designed stapled peptides in solution and in complex with eIF4E, supported with biophysical and crystallographic data. Clustering of the simulated structures revealed the favoured conformational states of the stapled peptides in their bound or free forms in solution. Identifying these populations has allowed us to design peptides with improved affinities by introducing mutations into the peptide sequence to alter their conformational distributions. These studies emphasise the effects that engineered mutations have on the conformations of free and bound peptides, and illustrate that both states must be considered in efforts to attain high affinity binding.

-	~~==Function==~~	+	Rational Optimization of Conformational Effects Induced By Hydrocarbon Staples in Peptides and their Binding Interfaces.,Lama D, Quah ST, Verma CS, Lakshminarayanan R, Beuerman RW, Lane DP, Brown CJ Sci Rep. 2013 Dec 13;3:3451. doi: 10.1038/srep03451. PMID:24336354<ref>PMID:24336354</ref>
-	~~[[http://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (~~By ~~similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step~~ in ~~the initiation of protein synthesis~~ and ~~facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures~~. ~~Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression~~. ~~In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap~~.<ref>PMID:~~16271312~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4bea]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4BEA OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024336354</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
-	[[Category: Brown, C J.]]	+	</StructureSection>
-	[[Category: Lama, D.]]	+	[[Category: Human]]
-	[[Category: Lane, D P.]]	+	[[Category: Brown, C J]]
-	[[Category: Quah, S T.]]	+	[[Category: Lama, D]]
-	[[Category: Verma, C S.]]	+	[[Category: Lane, D P]]
		+	[[Category: Quah, S T]]
		+	[[Category: Verma, C S]]
	[[Category: Translation]]		[[Category: Translation]]

OCA at 08:21, 8 January 2014

OCA — Wed, 08 Jan 2014 08:21:49 GMT

←Older revision		Revision as of 08:21, 8 January 2014
Line 1:		Line 1:
	{{STRUCTURE_4bea\| PDB=4bea \| SCENE= }}		{{STRUCTURE_4bea\| PDB=4bea \| SCENE= }}
	===Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative===		===Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative===
		+	{{ABSTRACT_PUBMED_24336354}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024336354</ref><references group="xtra"/><references/>
	[[Category: Brown, C J.]]		[[Category: Brown, C J.]]
	[[Category: Lama, D.]]		[[Category: Lama, D.]]

OCA at 13:43, 11 December 2013

OCA — Wed, 11 Dec 2013 13:43:53 GMT

←Older revision		Revision as of 13:43, 11 December 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bea\| PDB=4bea \| SCENE= }}
		+	===Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative===

-	~~The entry 4bea~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>

-	~~Authors~~: ~~Quah, S.T., Lama, D., Verma, C.S., Lane, D~~.P.~~, Brown, C~~.J.	+	==About this Structure==
		+	[[4bea]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEA OCA].

-	~~Description~~: ~~Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Brown, C J.]]
		+	[[Category: Lama, D.]]
		+	[[Category: Lane, D P.]]
		+	[[Category: Quah, S T.]]
		+	[[Category: Verma, C S.]]
		+	[[Category: Translation]]

OCA at 19:41, 17 April 2013

OCA — Wed, 17 Apr 2013 19:41:05 GMT

←Older revision		Revision as of 19:41, 17 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4bea is ON HOLD	+	The entry 4bea is ON HOLD until Paper Publication

	Authors: Quah, S.T., Lama, D., Verma, C.S., Lane, D.P., Brown, C.J.		Authors: Quah, S.T., Lama, D., Verma, C.S., Lane, D.P., Brown, C.J.

	Description: Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative		Description: Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative