4ber - Revision history

OCA at 11:49, 20 December 2023

OCA — Wed, 20 Dec 2023 11:49:36 GMT

←Older revision		Revision as of 11:49, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4ber' size='340' side='right'caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4ber' size='340' side='right'caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_33152 Atcc 33152~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BER FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BER FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [https://pdbe.org/4ber PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [https://www.ebi.ac.uk/pdbsum/4ber PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ber ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [https://pdbe.org/4ber PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [https://www.ebi.ac.uk/pdbsum/4ber PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ber ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>	+	[https://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33152]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Campanacci, V]]	+	[[Category: Legionella pneumophila]]
-	[[Category: Cherfils, J]]	+	[[Category: Campanacci V]]
-	[[Category: Mukherjee, S]]	+	[[Category: Cherfils J]]
-	[[Category: Roy~~, C R]]~~	+	[[Category: Mukherjee S]]
-	~~[[Category: Transferase]]~~	+	[[Category: Roy CR]]
-	~~[[Category: Type iv secretion system effector~~]]	+

OCA at 07:10, 31 August 2022

OCA — Wed, 31 Aug 2022 07:10:57 GMT

←Older revision		Revision as of 07:10, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4ber' size='340' side='right'caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4ber' size='340' side='right'caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BER FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BER FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [~~http~~://pdbe.org/4ber PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ber PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ber ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [https://pdbe.org/4ber PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [https://www.ebi.ac.uk/pdbsum/4ber PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ber ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>	+	[[https://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:00, 13 March 2019

OCA — Wed, 13 Mar 2019 14:00:22 GMT

←Older revision		Revision as of 14:00, 13 March 2019
Line 1:		Line 1:

	==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate==		==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate==
-	<StructureSection load='4ber' size='340' side='right' caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4ber' size='340' side='right'caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BER FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BER FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 33152]]		[[Category: Atcc 33152]]
		+	[[Category: Large Structures]]
	[[Category: Campanacci, V]]		[[Category: Campanacci, V]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]

OCA at 20:53, 5 August 2016

OCA — Fri, 05 Aug 2016 20:53:55 GMT

←Older revision		Revision as of 20:53, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate==		==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate==
	<StructureSection load='4ber' size='340' side='right' caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4ber' size='340' side='right' caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Legionella_pneumophila Legionella pneumophila~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BER FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BER FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [http://www.ebi.ac.uk/pdbsum/4ber PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [http://pdbe.org/4ber PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [http://www.ebi.ac.uk/pdbsum/4ber PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ber ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ber" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Legionella pneumophila~~]]	+	[[Category: Atcc 33152]]
	[[Category: Campanacci, V]]		[[Category: Campanacci, V]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]

OCA at 21:49, 25 December 2014

OCA — Thu, 25 Dec 2014 21:49:54 GMT

←Older revision		Revision as of 21:49, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [http://www.ebi.ac.uk/pdbsum/4ber PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [http://www.ebi.ac.uk/pdbsum/4ber PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:08, 21 December 2014

OCA — Sun, 21 Dec 2014 10:08:13 GMT

←Older revision		Revision as of 10:08, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4ber\| PDB=4ber \| SCENE= }}~~	+	==Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate==
-	===Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate===	+	<StructureSection load='4ber' size='340' side='right' caption='[[4ber]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23572077}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BER FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bep\|4bep]], [[4bes\|4bes]], [[4bet\|4bet]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ber FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ber OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ber RCSB], [http://www.ebi.ac.uk/pdbsum/4ber PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The FIC motif and the eukaryotic-like ankyrin repeats are found in many bacterial type IV effectors, yet little is known about how these domains enable bacteria to modulate host cell functions. Bacterial FIC domains typically bind ATP and transfer adenosine monophosphate moiety onto target proteins. The ankyrin repeat-containing protein AnkX encoded by the intracellular pathogen Legionella pneumophila is unique in that its FIC domain binds to CDP-choline and transfers a phosphocholine residue onto proteins in the Rab1 GTPase family. By determining the structures of unbound AnkX and AnkX with bound CDP-choline, CMP/phosphocholine and CMP, we demonstrate that the orientation of substrate binding in relation to the catalytic FIC motif enables this protein to function as a phosphocholinating enzyme rather than a nucleotidyl transferase. Additionally, the structure reveals that the ankyrin repeats mediate scaffolding interactions that resemble those found in protein-protein interactions, but are unprecedented in intramolecular interactions. Together with phosphocholination experiments, our structures unify a general phosphoryl transferase mechanism common to all FIC enzymes that should be conserved from bacteria to human.

-	~~==Function==~~	+	Structure of the Legionella effector AnkX reveals the mechanism of phosphocholine transfer by the FIC domain.,Campanacci V, Mukherjee S, Roy CR, Cherfils J EMBO J. 2013 Apr 9. doi: 10.1038/emboj.2013.82. PMID:23572077<ref>PMID:23572077</ref>
-	~~[[http://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence~~ effector ~~that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to~~ the ~~cytosolic face~~ of ~~the Legionella-containing vacuole (LCVs). Acts as a~~ phosphocholine ~~transferase~~ by ~~mediating~~ the ~~addition of phosphocholine to Ser residues of host RAB1 (RAB1A~~, ~~RAB1B or RAB1C) and RAB35~~, ~~leading to displacement of GDP dissociation inhibitors (GDI)~~. ~~Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB~~. ~~Can act on both GDP-bound and GTP-bound Rab proteins~~.~~<ref>PMID:18566289<~~/~~ref> <ref>~~PMID:~~22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref>~~ <ref>PMID:~~22411835~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4ber]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Legionella_pneumophila Legionella pneumophila]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023572077</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
-	[[Category: Campanacci, V.]]	+	[[Category: Campanacci, V]]
-	[[Category: Cherfils, J.]]	+	[[Category: Cherfils, J]]
-	[[Category: Mukherjee, S.]]	+	[[Category: Mukherjee, S]]
-	[[Category: Roy, C R.]]	+	[[Category: Roy, C R]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Type iv secretion system effector]]		[[Category: Type iv secretion system effector]]

OCA at 16:29, 19 June 2013

OCA — Wed, 19 Jun 2013 16:29:47 GMT

←Older revision		Revision as of 16:29, 19 June 2013
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	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:023572077</ref><references group="xtra"/><references/>
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
	[[Category: Campanacci, V.]]		[[Category: Campanacci, V.]]

OCA at 11:23, 24 April 2013

OCA — Wed, 24 Apr 2013 11:23:05 GMT

←Older revision		Revision as of 11:23, 24 April 2013
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4ber\| PDB=4ber \| SCENE= }}
		+	===Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate===
		+	{{ABSTRACT_PUBMED_23572077}}

-	~~The entry 4ber is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ANKX_LEGPH ANKX_LEGPH]] Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.<ref>PMID:18566289</ref> <ref>PMID:22158903</ref> <ref>PMID:21822290</ref> <ref>PMID:22307087</ref> <ref>PMID:22411835</ref>

-	~~Authors~~: ~~Campanacci, V~~.~~, Mukherjee, S~~.~~, Roy, C~~.R.~~, Cherfils, J~~.	+	==About this Structure==
		+	[[4ber]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BER OCA].

-	~~Description~~: ~~Crystal structure of the~~ Legionella pneumophila ~~FIC domain-containing~~ effector ~~AnkX protein in complex with cytidine monophosphate~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Legionella pneumophila]]
		+	[[Category: Campanacci, V.]]
		+	[[Category: Cherfils, J.]]
		+	[[Category: Mukherjee, S.]]
		+	[[Category: Roy, C R.]]
		+	[[Category: Transferase]]
		+	[[Category: Type iv secretion system effector]]

OCA at 00:36, 4 April 2013

OCA — Thu, 04 Apr 2013 00:36:46 GMT

←Older revision		Revision as of 00:36, 4 April 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ber is ON HOLD	+	The entry 4ber is ON HOLD until Paper Publication

	Authors: Campanacci, V., Mukherjee, S., Roy, C.R., Cherfils, J.		Authors: Campanacci, V., Mukherjee, S., Roy, C.R., Cherfils, J.

	Description: Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate		Description: Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein in complex with cytidine monophosphate

OCA: Protected "4ber" [edit=sysop:move=sysop]

OCA — Wed, 20 Mar 2013 16:05:57 GMT

Protected "4ber" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:05, 20 March 2013