4bir - Revision history

OCA at 06:49, 9 August 2023

OCA — Wed, 09 Aug 2023 06:49:48 GMT

←Older revision		Revision as of 06:49, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='4bir' size='340' side='right'caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4bir' size='340' side='right'caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aspoz Aspoz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BIR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BIR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SYNTHETIC GENE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~5062 ASPOZ])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [https://pdbe.org/4bir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [https://www.ebi.ac.uk/pdbsum/4bir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bir ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [https://pdbe.org/4bir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [https://www.ebi.ac.uk/pdbsum/4bir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bir ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNT1_ASPOR RNT1_ASPOR]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspoz~~]]	+	[[Category: Aspergillus oryzae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Doumen, J]]	+	[[Category: Doumen J]]
-	[[Category: Steyaert, J]]	+	[[Category: Steyaert J]]
-	~~[[Category: Endoribonuclease]]~~	+
-	~~[[Category: His to gln mutant]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ribonuclease~~]]	+

OCA at 15:48, 3 November 2021

OCA — Wed, 03 Nov 2021 15:48:44 GMT

←Older revision		Revision as of 15:48, 3 November 2021
Line 1:		Line 1:

	==RIBONUCLEASE T1: FREE HIS92GLN MUTANT==		==RIBONUCLEASE T1: FREE HIS92GLN MUTANT==
-	<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4bir' size='340' side='right'caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aspoz Aspoz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BIR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspoz Aspoz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BIR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [~~http~~://pdbe.org/4bir PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bir PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bir ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [https://pdbe.org/4bir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [https://www.ebi.ac.uk/pdbsum/4bir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bir ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]	+
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Aspoz]]		[[Category: Aspoz]]
		+	[[Category: Large Structures]]
	[[Category: Doumen, J]]		[[Category: Doumen, J]]
	[[Category: Steyaert, J]]		[[Category: Steyaert, J]]

OCA at 06:06, 18 April 2018

OCA — Wed, 18 Apr 2018 06:06:50 GMT

←Older revision		Revision as of 06:06, 18 April 2018
Line 1:		Line 1:
		+
	==RIBONUCLEASE T1: FREE HIS92GLN MUTANT==		==RIBONUCLEASE T1: FREE HIS92GLN MUTANT==
	<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [http://pdbe.org/4bir PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [http://www.ebi.ac.uk/pdbsum/4bir PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [http://pdbe.org/4bir PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [http://www.ebi.ac.uk/pdbsum/4bir PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bir ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/4bir_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/4bir_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:37, 9 February 2016

OCA — Tue, 09 Feb 2016 01:37:48 GMT

←Older revision		Revision as of 01:37, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspergillus_oryzae Aspergillus oryzae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BIR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspoz Aspoz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BIR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ~~Aspergillus oryzae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [http://www.ebi.ac.uk/pdbsum/4bir PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [http://pdbe.org/4bir PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [http://www.ebi.ac.uk/pdbsum/4bir PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4bir ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bir" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspergillus oryzae~~]]	+	[[Category: Aspoz]]
	[[Category: Doumen, J]]		[[Category: Doumen, J]]
	[[Category: Steyaert, J]]		[[Category: Steyaert, J]]

OCA at 08:03, 15 February 2015

OCA — Sun, 15 Feb 2015 08:03:19 GMT

←Older revision		Revision as of 08:03, 15 February 2015
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-	*[[~~User:Jaime.Prilusky/Test/tree~~\|~~User:Jaime.Prilusky/Test/tree~~]]	+	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:32, 9 December 2014

OCA — Tue, 09 Dec 2014 17:32:36 GMT

←Older revision		Revision as of 17:32, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4bir.~~png~~\|~~left~~\|200px]]	+	==RIBONUCLEASE T1: FREE HIS92GLN MUTANT==
		+	<StructureSection load='4bir' size='340' side='right' caption='[[4bir]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bir]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BIR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 Aspergillus oryzae])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bir OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bir RCSB], [http://www.ebi.ac.uk/pdbsum/4bir PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/4bir_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Histidine-40 is known to participate in phosphodiester transesterification catalyzed by the enzyme ribonuclease T1. A mutant enzyme with a lysine replacing the histidine-40 (His40Lys RNase T1) retains considerable catalytic activity [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. We report on the crystal structures of His40Lys RNase T1 containing a phosphate anion and a guanosine 2'-phosphate inhibitor in the active site, respectively. Similar to previously described structures, the phosphate-containing crystals are of space group P2(1)2(1)2(1), with one molecule per asymmetric unit (a = 48.27 A, b = 46.50 A, c = 41.14 A). The complex with 2'-GMP crystallized in the lower symmetry space group P2(1), with two molecules per asymmetric unit (a = 49.20 A, b = 48.19 A, c = 40.16 A, beta = 90.26). The crystal structures have been solved at 1.8- and 2.0-A resolution yielding R values of 14.5% and 16.0%, respectively. Comparison of these His40Lys structures with the corresponding wild-type structures, containing 2'-GMP [Arni, R., Heinemann, U., Tokuoka, R., & Saenger, W. (1988) J. Biol. Chem. 263, 15358-15368] and vanadate [Kostrewa, D., Hui-Woog Choe, Heinemann, U., & Saenger, W. (1989) Biochemistry 28, 7692-7600] in the active site, respectively, leads to the following conclusions. First, the His40Lys mutation causes no significant changes in the overall structure of RNase T1; second, the Lys40 side chains in the mutant structures occupy roughly the same space as His40 in the corresponding wild-type RNase T1 structures.(ABSTRACT TRUNCATED AT 250 WORDS)

-	~~{{STRUCTURE_4bir\| PDB=4bir \| SCENE= }}~~	+	Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant.,Zegers I, Verhelst P, Choe HW, Steyaert J, Heinemann U, Saenger W, Wyns L Biochemistry. 1992 Nov 24;31(46):11317-25. PMID:1445870<ref>PMID:1445870</ref>

-	~~===RIBONUCLEASE T1: FREE HIS92GLN MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_1445870}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4bir]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aspergillus_oryzae Aspergillus oryzae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:001445870<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
-	[[Category: Doumen, J.]]	+	[[Category: Doumen, J]]
-	[[Category: Steyaert, J.]]	+	[[Category: Steyaert, J]]
	[[Category: Endoribonuclease]]		[[Category: Endoribonuclease]]
	[[Category: His to gln mutant]]		[[Category: His to gln mutant]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ribonuclease]]		[[Category: Ribonuclease]]

OCA at 12:11, 20 October 2012

OCA — Sat, 20 Oct 2012 12:11:34 GMT

←Older revision		Revision as of 12:11, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[4bir]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].	+	[[4bir]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].

	==See Also==		==See Also==

OCA: Protected "4bir" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 07:54:54 GMT

Protected "4bir" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:54, 26 July 2012

OCA at 07:54, 26 July 2012

OCA — Thu, 26 Jul 2012 07:54:53 GMT

←Older revision		Revision as of 07:54, 26 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:4bir.png\|left\|200px]]		[[Image:4bir.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4bir", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4bir\| PDB=4bir \| SCENE= }}		{{STRUCTURE_4bir\| PDB=4bir \| SCENE= }}

	===RIBONUCLEASE T1: FREE HIS92GLN MUTANT===		===RIBONUCLEASE T1: FREE HIS92GLN MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1445870}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1445870 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_1445870}}		{{ABSTRACT_PUBMED_1445870}}

	==About this Structure==		==About this Structure==
-	~~4BIR~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].	+	[[4bir]] is a 1 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].
		+
		+	==See Also==
		+	*[[Ribonuclease\|Ribonuclease]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1445870~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001445870</ref><references group="xtra"/>
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
	[[Category: Doumen, J.]]		[[Category: Doumen, J.]]
Line 31:		Line 22:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ribonuclease]]		[[Category: Ribonuclease]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:24:45 2009''

OCA at 03:24, 17 February 2009

OCA — Tue, 17 Feb 2009 03:24:46 GMT

←Older revision		Revision as of 03:24, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	4BIR is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].	+	4BIR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BIR OCA].

	==Reference==		==Reference==
-	Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant., Zegers I, Verhelst P, Choe HW, Steyaert J, Heinemann U, Saenger W, Wyns L, Biochemistry. 1992 Nov 24;31(46):11317-25. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/1445870 1445870]~~	+	<ref group="xtra">PMID:1445870</ref><references group="xtra"/>
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
-	[[Category: Single protein]]
	[[Category: Doumen, J.]]		[[Category: Doumen, J.]]
	[[Category: Steyaert, J.]]		[[Category: Steyaert, J.]]
Line 33:		Line 32:
	[[Category: Ribonuclease]]		[[Category: Ribonuclease]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:29:~~41 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:24:45 2009''