4bq4 - Revision history

OCA at 11:56, 20 December 2023

OCA — Wed, 20 Dec 2023 11:56:35 GMT

←Older revision		Revision as of 11:56, 20 December 2023
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	<StructureSection load='4bq4' size='340' side='right'caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4bq4' size='340' side='right'caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=AAL:3,6~~-~~ANHYDRO-L-GALACTOSE'>AAL</scene>~~, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [https://pdbe.org/4bq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4bq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [https://pdbe.org/4bq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4bq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q21HC5_SACD2 Q21HC5_SACD2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-agarase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Boraston, A B~~]]	+	[[Category: Saccharophagus degradans 2-40]]
-	[[Category: ~~Hehemann, J H~~]]	+	[[Category: Boraston AB]]
-	[[Category: ~~Pluvinage, B~~]]	+	[[Category: Hehemann JH]]
-	[[Category: ~~Hydrolase~~]]	+	[[Category: Pluvinage B]]

OCA at 07:21, 31 August 2022

OCA — Wed, 31 Aug 2022 07:21:42 GMT

←Older revision		Revision as of 07:21, 31 August 2022
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	<StructureSection load='4bq4' size='340' side='right'caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4bq4' size='340' side='right'caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~AAL~~:~~3,6-ANHYDRO-L-GALACTOSE~~'>~~AAL~~</scene>, <scene name='pdbligand=CA:~~CALCIUM+ION~~'>CA</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-GALACTOSE'>~~GAL~~</scene>, <scene name='pdbligand=~~GOL~~:~~GLYCEROL~~'>~~GOL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [~~http~~://pdbe.org/4bq4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bq4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [https://pdbe.org/4bq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4bq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	[[Category: Beta-agarase]]		[[Category: Beta-agarase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Microbulbifer degradans 2-40]]
	[[Category: Boraston, A B]]		[[Category: Boraston, A B]]
	[[Category: Hehemann, J H]]		[[Category: Hehemann, J H]]
	[[Category: Pluvinage, B]]		[[Category: Pluvinage, B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 14:00, 13 March 2019

OCA — Wed, 13 Mar 2019 14:00:45 GMT

←Older revision		Revision as of 14:00, 13 March 2019
Line 1:		Line 1:

	==Structural analysis of an exo-beta-agarase==		==Structural analysis of an exo-beta-agarase==
-	<StructureSection load='4bq4' size='340' side='right' caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='4bq4' size='340' side='right'caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:~~CALCIUM+ION~~'>CA</scene>, <scene name='pdbligand=~~GOL~~:~~GLYCEROL~~'>~~GOL~~</scene>, <scene name='pdbligand=~~AAL~~:~~3,6~~-~~ANHYDRO-L~~-GALACTOSE'>~~AAL~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Beta-agarase]]		[[Category: Beta-agarase]]
		+	[[Category: Large Structures]]
	[[Category: Microbulbifer degradans 2-40]]		[[Category: Microbulbifer degradans 2-40]]
	[[Category: Boraston, A B]]		[[Category: Boraston, A B]]

OCA at 05:11, 5 August 2016

OCA — Fri, 05 Aug 2016 05:11:43 GMT

←Older revision		Revision as of 05:11, 5 August 2016
Line 1:		Line 1:
		+
	==Structural analysis of an exo-beta-agarase==		==Structural analysis of an exo-beta-agarase==
	<StructureSection load='4bq4' size='340' side='right' caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4bq4' size='340' side='right' caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharophagus_degradans_2~~-40 ~~Saccharophagus~~ degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bq4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [http://pdbe.org/4bq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bq4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Beta-agarase]]		[[Category: Beta-agarase]]
-	[[Category: ~~Saccharophagus~~ degradans 2-40]]	+	[[Category: Microbulbifer degradans 2-40]]
	[[Category: Boraston, A B]]		[[Category: Boraston, A B]]
	[[Category: Hehemann, J H]]		[[Category: Hehemann, J H]]
	[[Category: Pluvinage, B]]		[[Category: Pluvinage, B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:08, 21 December 2014

OCA — Sun, 21 Dec 2014 10:08:36 GMT

←Older revision		Revision as of 10:08, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bq4~~\| ~~PDB~~=4bq4 \| ~~SCENE~~= }}	+	==Structural analysis of an exo-beta-agarase==
-	===~~Structural analysis of an exo~~-~~beta~~-agarase===	+	<StructureSection load='4bq4' size='340' side='right' caption='[[4bq4]], [[Resolution\|resolution]] 2.05Å' scene=''>
-	{{~~ABSTRACT_PUBMED_23921382}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bq2\|4bq2]], [[4bq3\|4bq3]], [[4bq5\|4bq5]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bq4 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacteria that metabolize agarose use multiple enzymes of complementary specificities to hydrolyze the glycosidic linkages in agarose, a linear polymer comprising the repeating disaccharide subunit of neoagarobiose (3,6-anhydro-L-galactose-alpha-(1,3)-D-galactose), which are beta-(1,4)-linked. Here we present the crystal structure of a glycoside hydrolase family 50 exo-beta-agarase Aga50D from the marine microbe Saccharophagus degradans. This enzyme catalyzes a critical step in the metabolism of agarose by S. degradans through cleaving agarose oligomers into neoagarobiose products that can be further processed into monomers. The crystal structure of Aga50D to 1.9 &{Aring] resolution reveals a (beta/alpha)8-barrel fold that is elaborated with a beta-sandwich domain and extensive loops. The structure of catalytically inactivated Aga50D in complex with non-hydrolyzed neoagarotetraose (2.05 &{Aring] resolution) and neoagarooctaose (2.30 &{Aring] resolution) provide views of Michaelis complexes for a beta-agarase. In these structures, the D-galactose residue in the -1 subsite is distorted into a 1S3 skew boat conformation. The relative positioning of the putative catalytic residues are most consistent with a retaining catalytic mechanism. Additionally, the neoagarooctaose complex showed that this extended substrate made substantial interactions with the beta-sandwich domain, which resembles a carbohydrate binding module, thus creating additional plus (+) subsites and funneling the polymeric substrate through the tunnel shaped active site. A synthesis of these results in combination with an additional neoagarobiose product complex suggests a potential exo-processive mode of action of Aga50D on the agarose double helix.

-	~~==About this Structure==~~	+	Substrate recognition and hydrolysis by a family 50 exo-beta-agarase Aga50D from the marine bacterium Saccharophagus degradans.,Pluvinage B, Hehemann JH, Boraston AB J Biol Chem. 2013 Aug 6. PMID:23921382<ref>PMID:23921382</ref>
-	~~[[4bq4]] is~~ a ~~2 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40~~ Saccharophagus degradans ~~2-40]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4BQ4 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023921382</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Beta-agarase]]		[[Category: Beta-agarase]]
	[[Category: Saccharophagus degradans 2-40]]		[[Category: Saccharophagus degradans 2-40]]
-	[[Category: Boraston, A B.]]	+	[[Category: Boraston, A B]]
-	[[Category: Hehemann, J H.]]	+	[[Category: Hehemann, J H]]
-	[[Category: Pluvinage, B.]]	+	[[Category: Pluvinage, B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:27, 21 August 2013

OCA — Wed, 21 Aug 2013 08:27:21 GMT

←Older revision		Revision as of 08:27, 21 August 2013
Line 1:		Line 1:
	{{STRUCTURE_4bq4\| PDB=4bq4 \| SCENE= }}		{{STRUCTURE_4bq4\| PDB=4bq4 \| SCENE= }}
	===Structural analysis of an exo-beta-agarase===		===Structural analysis of an exo-beta-agarase===
		+	{{ABSTRACT_PUBMED_23921382}}

	==About this Structure==		==About this Structure==
	[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA].		[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023921382</ref><references group="xtra"/><references/>
	[[Category: Beta-agarase]]		[[Category: Beta-agarase]]
	[[Category: Saccharophagus degradans 2-40]]		[[Category: Saccharophagus degradans 2-40]]

OCA at 07:57, 14 August 2013

OCA — Wed, 14 Aug 2013 07:57:43 GMT

←Older revision		Revision as of 07:57, 14 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bq4\| PDB=4bq4 \| SCENE= }}
		+	===Structural analysis of an exo-beta-agarase===

-	~~The entry~~ 4bq4 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4bq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ4 OCA].
-	~~Authors~~: ~~Pluvinage~~, B., Hehemann, J.H., ~~Boraston, A.~~B.	+	[[Category: Beta-agarase]]
-		+	[[Category: Saccharophagus degradans 2-40]]
-	~~Description~~: ~~Structural analysis of an exo-beta-agarase~~	+	[[Category: Boraston, A B.]]
		+	[[Category: Hehemann, J H.]]
		+	[[Category: Pluvinage, B.]]
		+	[[Category: Hydrolase]]

OCA: Protected "4bq4" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 10:53:42 GMT

Protected "4bq4" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4bq4 is ON HOLD until Paper Publication Authors: Pluvinage, B., Hehemann, J.H., Boraston, A.B. Description: Structural analysis of an exo-beta-agar...

OCA — Wed, 19 Jun 2013 10:53:39 GMT

New page: '''Unreleased structure''' The entry 4bq4 is ON HOLD until Paper Publication Authors: Pluvinage, B., Hehemann, J.H., Boraston, A.B. Description: Structural analysis of an exo-beta-agar...

New page

'''Unreleased structure'''

The entry 4bq4 is ON HOLD until Paper Publication

Authors: Pluvinage, B., Hehemann, J.H., Boraston, A.B.

Description: Structural analysis of an exo-beta-agarase