4bt0 - Revision history

OCA at 11:04, 9 May 2024

OCA — Thu, 09 May 2024 11:04:52 GMT

←Older revision		Revision as of 11:04, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 17Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [https://pdbe.org/4bt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [https://pdbe.org/4bt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/O67198_AQUAE O67198_AQUAE]]	+	[https://www.uniprot.org/uniprot/O67198_AQUAE O67198_AQUAE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MuB is an ATP-dependent nonspecific DNA-binding protein that regulates the activity of the MuA transposase and captures target DNA for transposition. Mechanistic understanding of MuB function has previously been hindered by MuB's poor solubility. Here we combine bioinformatic, mutagenic, biochemical, and electron microscopic analyses to unmask the structure and function of MuB. We demonstrate that MuB is an ATPase associated with diverse cellular activities (AAA+ ATPase) and forms ATP-dependent filaments with or without DNA. We also identify critical residues for MuB's ATPase, DNA binding, protein polymerization, and MuA interaction activities. Using single-particle electron microscopy, we show that MuB assembles into a helical filament, which binds the DNA in the axial channel. The helical parameters of the MuB filament do not match those of the coated DNA. Despite this protein-DNA symmetry mismatch, MuB does not deform the DNA duplex. These findings, together with the influence of MuB filament size on strand-transfer efficiency, lead to a model in which MuB-imposed symmetry transiently deforms the DNA at the boundary of the MuB filament and results in a bent DNA favored by MuA for transposition.
		+
		+	MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition.,Mizuno N, Dramicanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramon-Maiques S Proc Natl Acad Sci U S A. 2013 Jun 17. PMID:23776210<ref>PMID:23776210</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bt0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 17:06, 7 September 2022

OCA — Wed, 07 Sep 2022 17:06:13 GMT

←Older revision		Revision as of 17:06, 7 September 2022
Line 3:		Line 3:
	<SX load='4bt0' size='340' side='right' viewer='molstar' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>		<SX load='4bt0' size='340' side='right' viewer='molstar' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpmu Bpmu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [https://pdbe.org/4bt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [~~http~~://pdbe.org/4bt0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/O67198_AQUAE O67198_AQUAE]]
-	MuB is an ATP-dependent nonspecific DNA-binding protein that regulates the activity of the MuA transposase and captures target DNA for transposition. Mechanistic understanding of MuB function has previously been hindered by MuB's poor solubility. Here we combine bioinformatic, mutagenic, biochemical, and electron microscopic analyses to unmask the structure and function of MuB. We demonstrate that MuB is an ATPase associated with diverse cellular activities (AAA+ ATPase) and forms ATP-dependent filaments with or without DNA. We also identify critical residues for MuB's ATPase, DNA binding, protein polymerization, and MuA interaction activities. Using single-particle electron microscopy, we show that MuB assembles into a helical filament, which binds the DNA in the axial channel. The helical parameters of the MuB filament do not match those of the coated DNA. Despite this protein-DNA symmetry mismatch, MuB does not deform the DNA duplex. These findings, together with the influence of MuB filament size on strand-transfer efficiency, lead to a model in which MuB-imposed symmetry transiently deforms the DNA at the boundary of the MuB filament and results in a bent DNA favored by MuA for transposition.	+
-		+
-	MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition.,Mizuno N, Dramicanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramon-Maiques S Proc Natl Acad Sci U S A. 2013 Jun 17. PMID:~~23776210<ref>PMID:23776210<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4bt0" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Bpmu~~]]	+	[[Category: Escherichia virus Mu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adam, J]]	+	[[Category: Adam J]]
-	[[Category: Dramicanin, M]]	+	[[Category: Dramicanin M]]
-	[[Category: Han~~, Y W~~]]	+	[[Category: Han YW]]
-	[[Category: Mizuno, N]]	+	[[Category: Mizuno N]]
-	[[Category: Mizuuchi, K]]	+	[[Category: Mizuuchi K]]
-	[[Category: Mizuuchi, M]]	+	[[Category: Mizuuchi M]]
-	[[Category: Ramon-Maiques, S]]	+	[[Category: Ramon-Maiques S]]
-	[[Category: Steven~~, A C~~]]	+	[[Category: Steven AC]]
-	[[Category: Wang, Y]]	+	[[Category: Wang Y]]
-	[[Category: Yang, W]]	+	[[Category: Yang W]]
-	~~[[Category: Aaa+ atpase]]~~	+
-	~~[[Category: Dna transposition]]~~	+
-	~~[[Category: Nucleoprotein filament]]~~	+
-	~~[[Category: Symmetry mismatch]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 18:57, 10 April 2020

OCA — Fri, 10 Apr 2020 18:57:03 GMT

←Older revision		Revision as of 18:57, 10 April 2020
Line 3:		Line 3:
	<SX load='4bt0' size='340' side='right' viewer='molstar' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>		<SX load='4bt0' size='340' side='right' viewer='molstar' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [http://pdbe.org/4bt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [http://pdbe.org/4bt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:51, 6 March 2020

OCA — Fri, 06 Mar 2020 17:51:12 GMT

←Older revision		Revision as of 17:51, 6 March 2020
Line 1:		Line 1:

	==MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition==		==MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition==
-	<~~StructureSection~~ load='4bt0' size='340' side='right'caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>	+	<SX load='4bt0' size='340' side='right' viewer='molstar' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bpmu]]		[[Category: Bpmu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 16:52, 28 August 2019

OCA — Wed, 28 Aug 2019 16:52:27 GMT

←Older revision		Revision as of 16:52, 28 August 2019
Line 1:		Line 1:

	==MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition==		==MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition==
-	<StructureSection load='4bt0' size='340' side='right' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>	+	<StructureSection load='4bt0' size='340' side='right'caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bpmu]]		[[Category: Bpmu]]
		+	[[Category: Large Structures]]
	[[Category: Adam, J]]		[[Category: Adam, J]]
	[[Category: Dramicanin, M]]		[[Category: Dramicanin, M]]

OCA at 22:17, 15 November 2017

OCA — Wed, 15 Nov 2017 22:17:54 GMT

←Older revision		Revision as of 22:17, 15 November 2017
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [http://pdbe.org/4bt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [http://pdbe.org/4bt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:34, 11 May 2016

OCA — Wed, 11 May 2016 05:34:28 GMT

←Older revision		Revision as of 05:34, 11 May 2016
Line 1:		Line 1:
-	{{STRUCTURE_4bt0\| PDB=4bt0 \| SCENE= }}
-	===MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition===
-	{{ABSTRACT_PUBMED_23776210}}

-	==~~About this Structure~~==	+	==MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition==
-	[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_mu Enterobacteria phage mu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA].	+	<StructureSection load='4bt0' size='340' side='right' caption='[[4bt0]], [[Resolution\|resolution]] 17.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BT0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bs1\|4bs1]], [[4bt1\|4bt1]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bt0 OCA], [http://pdbe.org/4bt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bt0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bt0 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MuB is an ATP-dependent nonspecific DNA-binding protein that regulates the activity of the MuA transposase and captures target DNA for transposition. Mechanistic understanding of MuB function has previously been hindered by MuB's poor solubility. Here we combine bioinformatic, mutagenic, biochemical, and electron microscopic analyses to unmask the structure and function of MuB. We demonstrate that MuB is an ATPase associated with diverse cellular activities (AAA+ ATPase) and forms ATP-dependent filaments with or without DNA. We also identify critical residues for MuB's ATPase, DNA binding, protein polymerization, and MuA interaction activities. Using single-particle electron microscopy, we show that MuB assembles into a helical filament, which binds the DNA in the axial channel. The helical parameters of the MuB filament do not match those of the coated DNA. Despite this protein-DNA symmetry mismatch, MuB does not deform the DNA duplex. These findings, together with the influence of MuB filament size on strand-transfer efficiency, lead to a model in which MuB-imposed symmetry transiently deforms the DNA at the boundary of the MuB filament and results in a bent DNA favored by MuA for transposition.

-	~~==Reference==~~	+	MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition.,Mizuno N, Dramicanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramon-Maiques S Proc Natl Acad Sci U S A. 2013 Jun 17. PMID:23776210<ref>PMID:23776210</ref>
-	<ref ~~group="xtra"~~>PMID:~~023776210~~</ref><~~references group~~="~~xtra~~"/><references/>	+
-	[[Category: ~~Enterobacteria phage mu~~]]	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: Adam, J.]]	+	</div>
-	[[Category: Dramicanin, M.]]	+	<div class="pdbe-citations 4bt0" style="background-color:#fffaf0;"></div>
-	[[Category: Han, Y W.]]	+	== References ==
-	[[Category: Mizuno, N.]]	+	<references/>
-	[[Category: Mizuuchi, K.]]	+	__TOC__
-	[[Category: Mizuuchi, M.]]	+	</StructureSection>
-	[[Category: Ramon-Maiques, S.]]	+	[[Category: Bpmu]]
-	[[Category: Steven, A C.]]	+	[[Category: Adam, J]]
-	[[Category: Wang, Y.]]	+	[[Category: Dramicanin, M]]
-	[[Category: Yang, W.]]	+	[[Category: Han, Y W]]
		+	[[Category: Mizuno, N]]
		+	[[Category: Mizuuchi, K]]
		+	[[Category: Mizuuchi, M]]
		+	[[Category: Ramon-Maiques, S]]
		+	[[Category: Steven, A C]]
		+	[[Category: Wang, Y]]
		+	[[Category: Yang, W]]
	[[Category: Aaa+ atpase]]		[[Category: Aaa+ atpase]]
	[[Category: Dna transposition]]		[[Category: Dna transposition]]

OCA at 14:03, 3 July 2013

OCA — Wed, 03 Jul 2013 14:03:59 GMT

←Older revision		Revision as of 14:03, 3 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bt0\| PDB=4bt0 \| SCENE= }}
		+	===MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition===
		+	{{ABSTRACT_PUBMED_23776210}}

-	~~The entry~~ 4bt0 is ~~ON HOLD until sometime in the future~~	+	==About this Structure==
		+	[[4bt0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BT0 OCA].

-	~~Authors~~: ~~Mizuno~~, N., Dramicanin, M., ~~Mizuuchi, M~~., ~~Adam, J~~., ~~Wang, Y~~., ~~Han, Y.W~~., ~~Yang, W~~., Steven, A.C., ~~Mizuuchi, K~~., ~~Ramon-Maiques, S~~.	+	==Reference==
-		+	<ref group="xtra">PMID:023776210</ref><references group="xtra"/><references/>
-	~~Description~~: ~~MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA~~ transposition	+	[[Category: Enterobacteria phage mu]]
		+	[[Category: Adam, J.]]
		+	[[Category: Dramicanin, M.]]
		+	[[Category: Han, Y W.]]
		+	[[Category: Mizuno, N.]]
		+	[[Category: Mizuuchi, K.]]
		+	[[Category: Mizuuchi, M.]]
		+	[[Category: Ramon-Maiques, S.]]
		+	[[Category: Steven, A C.]]
		+	[[Category: Wang, Y.]]
		+	[[Category: Yang, W.]]
		+	[[Category: Aaa+ atpase]]
		+	[[Category: Dna transposition]]
		+	[[Category: Nucleoprotein filament]]
		+	[[Category: Symmetry mismatch]]
		+	[[Category: Transcription]]

OCA: Protected "4bt0" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 10:58:14 GMT

Protected "4bt0" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:58, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4bt0 is ON HOLD until sometime in the future Authors: Mizuno, N., Dramicanin, M., Mizuuchi, M., Adam, J., Wang, Y., Han, Y.W., Yang, W., Steven, A.C...

OCA — Wed, 19 Jun 2013 10:58:13 GMT

New page: '''Unreleased structure''' The entry 4bt0 is ON HOLD until sometime in the future Authors: Mizuno, N., Dramicanin, M., Mizuuchi, M., Adam, J., Wang, Y., Han, Y.W., Yang, W., Steven, A.C...

New page

'''Unreleased structure'''

The entry 4bt0 is ON HOLD until sometime in the future

Authors: Mizuno, N., Dramicanin, M., Mizuuchi, M., Adam, J., Wang, Y., Han, Y.W., Yang, W., Steven, A.C., Mizuuchi, K., Ramon-Maiques, S.

Description: MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition