4btg - Revision history

OCA at 11:05, 9 May 2024

OCA — Thu, 09 May 2024 11:05:06 GMT

←Older revision		Revision as of 11:05, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BTG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [https://pdbe.org/4btg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [https://www.ebi.ac.uk/pdbsum/4btg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [https://pdbe.org/4btg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [https://www.ebi.ac.uk/pdbsum/4btg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.	+	[https://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.
		+
		+	Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4btg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 17:06, 7 September 2022

OCA — Wed, 07 Sep 2022 17:06:43 GMT

←Older revision		Revision as of 17:06, 7 September 2022
Line 3:		Line 3:
	<SX load='4btg' size='340' side='right' viewer='molstar' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='4btg' size='340' side='right' viewer='molstar' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpph6 Bpph6~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4BTG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [~~http~~://pdbe.org/4btg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4btg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [https://pdbe.org/4btg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [https://www.ebi.ac.uk/pdbsum/4btg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.	+	[[https://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.	+
-		+
-	Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4btg" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Bpph6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boura, E]]	+	[[Category: Pseudomonas virus phi6]]
-	[[Category: Cheng, N]]	+	[[Category: Boura E]]
-	[[Category: Heymann~~, J B~~]]	+	[[Category: Cheng N]]
-	[[Category: Hurley~~, J H~~]]	+	[[Category: Heymann JB]]
-	[[Category: Mindich, L]]	+	[[Category: Hurley JH]]
-	[[Category: Nemecek, D]]	+	[[Category: Mindich L]]
-	[[Category: Plevka, P]]	+	[[Category: Nemecek D]]
-	[[Category: Qiao, J]]	+	[[Category: Plevka P]]
-	[[Category: Steven~~, A C~~]]	+	[[Category: Qiao J]]
-	[[Category: Wu, W]]	+	[[Category: Steven AC]]
-	~~[[Category: Cystoviridae]]~~	+	[[Category: Wu W]]
-	~~[[Category: Flexible fitting]]~~	+
-	~~[[Category: Procapsid structure]]~~	+
-	~~[[Category: Virus~~]]	+

OCA at 18:57, 10 April 2020

OCA — Fri, 10 Apr 2020 18:57:28 GMT

←Older revision		Revision as of 18:57, 10 April 2020
Line 3:		Line 3:
	<SX load='4btg' size='340' side='right' viewer='molstar' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='4btg' size='340' side='right' viewer='molstar' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BTG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://pdbe.org/4btg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://pdbe.org/4btg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:51, 6 March 2020

OCA — Fri, 06 Mar 2020 17:51:20 GMT

←Older revision		Revision as of 17:51, 6 March 2020
Line 1:		Line 1:

	==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==		==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==
-	<~~StructureSection~~ load='4btg' size='340' side='right'caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>	+	<SX load='4btg' size='340' side='right' viewer='molstar' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bpph6]]		[[Category: Bpph6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 16:22, 28 August 2019

OCA — Wed, 28 Aug 2019 16:22:15 GMT

←Older revision		Revision as of 16:22, 28 August 2019
Line 1:		Line 1:

	==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==		==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==
-	<StructureSection load='4btg' size='340' side='right' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>	+	<StructureSection load='4btg' size='340' side='right'caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bpph6]]		[[Category: Bpph6]]
		+	[[Category: Large Structures]]
	[[Category: Boura, E]]		[[Category: Boura, E]]
	[[Category: Cheng, N]]		[[Category: Cheng, N]]

OCA at 14:30, 5 August 2016

OCA — Fri, 05 Aug 2016 14:30:00 GMT

←Older revision		Revision as of 14:30, 5 August 2016
Line 1:		Line 1:
		+
	==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==		==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==
	<StructureSection load='4btg' size='340' side='right' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='4btg' size='340' side='right' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_phage_phi6 Pseudomonas phage phi6~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://pdbe.org/4btg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4btg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4btg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas phage phi6~~]]	+	[[Category: Bpph6]]
	[[Category: Boura, E]]		[[Category: Boura, E]]
	[[Category: Cheng, N]]		[[Category: Cheng, N]]

OCA at 19:34, 25 December 2014

OCA — Thu, 25 Dec 2014 19:34:59 GMT

←Older revision		Revision as of 19:34, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:00, 21 December 2014

OCA — Sun, 21 Dec 2014 09:00:17 GMT

←Older revision		Revision as of 09:00, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4btg\| PDB=4btg \| SCENE= }}~~	+	==Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution==
-	===Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution===	+	<StructureSection load='4btg' size='340' side='right' caption='[[4btg]], [[Resolution\|resolution]] 4.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23891288}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTG FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btg RCSB], [http://www.ebi.ac.uk/pdbsum/4btg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.

-	~~==Function==~~	+	Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref>
-	~~[[http://www~~.~~uniprot~~.~~org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid~~ (~~core~~) ~~protein of the polyhedral procapsid, which is responsible for genomic replication and transcription~~. ~~Forms a dodecahedral shell from 60 asymmetric dimers~~. ~~Binds to RNA and may be involved in genomic packaging~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4btg]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023891288</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas phage phi6]]		[[Category: Pseudomonas phage phi6]]
-	[[Category: Boura, E.]]	+	[[Category: Boura, E]]
-	[[Category: Cheng, N.]]	+	[[Category: Cheng, N]]
-	[[Category: Heymann, J B.]]	+	[[Category: Heymann, J B]]
-	[[Category: Hurley, J H.]]	+	[[Category: Hurley, J H]]
-	[[Category: Mindich, L.]]	+	[[Category: Mindich, L]]
-	[[Category: Nemecek, D.]]	+	[[Category: Nemecek, D]]
-	[[Category: Plevka, P.]]	+	[[Category: Plevka, P]]
-	[[Category: Qiao, J.]]	+	[[Category: Qiao, J]]
-	[[Category: Steven, A C.]]	+	[[Category: Steven, A C]]
-	[[Category: Wu, W.]]	+	[[Category: Wu, W]]
	[[Category: Cystoviridae]]		[[Category: Cystoviridae]]
	[[Category: Flexible fitting]]		[[Category: Flexible fitting]]
	[[Category: Procapsid structure]]		[[Category: Procapsid structure]]
	[[Category: Virus]]		[[Category: Virus]]

OCA at 07:53, 14 August 2013

OCA — Wed, 14 Aug 2013 07:53:46 GMT

←Older revision		Revision as of 07:53, 14 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4btg\| PDB=4btg \| SCENE= }}
		+	===Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution===
		+	{{ABSTRACT_PUBMED_23891288}}

-	~~The entry 4btg~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.

-	~~Authors~~: ~~Nemecek, D~~.~~, Boura, E~~.~~, Wu, W~~.~~, Cheng, N., Plevka, P., Qiao, J., Mindich, L., Heymann, J.B., Hurley, J~~.H.~~, Steven, A~~.C.	+	==About this Structure==
		+	[[4btg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTG OCA].

-	~~Description~~: ~~Coordinates of the bacteriophage~~ phi6 ~~capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4~~.4 A ~~resolution~~	+	==Reference==
		+	<ref group="xtra">PMID:023891288</ref><references group="xtra"/><references/>
		+	[[Category: Pseudomonas phage phi6]]
		+	[[Category: Boura, E.]]
		+	[[Category: Cheng, N.]]
		+	[[Category: Heymann, J B.]]
		+	[[Category: Hurley, J H.]]
		+	[[Category: Mindich, L.]]
		+	[[Category: Nemecek, D.]]
		+	[[Category: Plevka, P.]]
		+	[[Category: Qiao, J.]]
		+	[[Category: Steven, A C.]]
		+	[[Category: Wu, W.]]
		+	[[Category: Cystoviridae]]
		+	[[Category: Flexible fitting]]
		+	[[Category: Procapsid structure]]
		+	[[Category: Virus]]

OCA: Protected "4btg" [edit=sysop:move=sysop]

OCA — Sun, 30 Jun 2013 08:29:14 GMT

Protected "4btg" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:29, 30 June 2013