4bup - Revision history

OCA at 11:58, 20 December 2023

OCA — Wed, 20 Dec 2023 11:58:19 GMT

←Older revision		Revision as of 11:58, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BUP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BUP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.166Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [https://pdbe.org/4bup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [https://www.ebi.ac.uk/pdbsum/4bup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bup ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [https://pdbe.org/4bup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [https://www.ebi.ac.uk/pdbsum/4bup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bup ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KMT5B_MOUSE KMT5B_MOUSE]] Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:28114273, PubMed:24049080, PubMed:15145825). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression (PubMed:15145825). Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions (PubMed:15145825). KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (PubMed:16612004, PubMed:15750587). Plays a role in myogenesis by regulating the expression of target genes, such as EID3 (PubMed:23720823). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273).[UniProtKB:Q4FZB7]<ref>PMID:15145825</ref> <ref>PMID:15750587</ref> <ref>PMID:16612004</ref> <ref>PMID:23720823</ref> <ref>PMID:24049080</ref> <ref>PMID:28114273</ref>	+	[https://www.uniprot.org/uniprot/KMT5B_MOUSE KMT5B_MOUSE] Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:28114273, PubMed:24049080, PubMed:15145825). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression (PubMed:15145825). Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions (PubMed:15145825). KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (PubMed:16612004, PubMed:15750587). Plays a role in myogenesis by regulating the expression of target genes, such as EID3 (PubMed:23720823). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273).[UniProtKB:Q4FZB7]<ref>PMID:15145825</ref> <ref>PMID:15750587</ref> <ref>PMID:16612004</ref> <ref>PMID:23720823</ref> <ref>PMID:24049080</ref> <ref>PMID:28114273</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The delivery of site-specific post-translational modifications to histones generates an epigenetic regulatory network that directs fundamental DNA-mediated processes and governs key stages in development. Methylation of histone H4 lysine-20 has been implicated in DNA repair, transcriptional silencing, genomic stability and regulation of replication. We present the structure of the histone H4K20 methyltransferase Suv4-20h2 in complex with its histone H4 peptide substrate and S-adenosyl methionine cofactor. Analysis of the structure reveals that the Suv4-20h2 active site diverges from the canonical SET domain configuration and generates a high degree of both substrate and product specificity. Together with supporting biochemical data comparing Suv4-20h1 and Suv4-20h2, we demonstrate that the Suv4-20 family enzymes take a previously mono-methylated H4K20 substrate and generate an exclusively di-methylated product. We therefore predict that other enzymes are responsible for the tri-methylation of histone H4K20 that marks silenced heterochromatin.
		+
		+	A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases.,Southall SM, Cronin NB, Wilson JR Nucleic Acids Res. 2013 Sep 18. PMID:24049080<ref>PMID:24049080</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bup" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 17:09, 7 September 2022

OCA — Wed, 07 Sep 2022 17:09:05 GMT

←Older revision		Revision as of 17:09, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4bup' size='340' side='right'caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>		<StructureSection load='4bup' size='340' side='right'caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BUP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BUP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4au7\|4au7]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [https://pdbe.org/4bup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [https://www.ebi.ac.uk/pdbsum/4bup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bup ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [~~http~~://pdbe.org/4bup PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bup PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bup ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/~~SV421_MOUSE SV421_MOUSE~~]] Histone methyltransferase that specifically ~~trimethylates~~ 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. ~~SUV420H1~~ is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2)~~.<ref>PMID~~:~~15145825</ref>~~	+	[[https://www.uniprot.org/uniprot/KMT5B_MOUSE KMT5B_MOUSE]] Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:28114273, PubMed:24049080, PubMed:15145825). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression (PubMed:15145825). Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions (PubMed:15145825). KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (PubMed:16612004, PubMed:15750587). Plays a role in myogenesis by regulating the expression of target genes, such as EID3 (PubMed:23720823). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273).[UniProtKB:Q4FZB7]<ref>PMID:15145825</ref> <ref>PMID:15750587</ref> <ref>PMID:16612004</ref> <ref>PMID:23720823</ref> <ref>PMID:24049080</ref> <ref>PMID:28114273</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from~~ PubMed ==	+
-	~~The delivery~~ of ~~site~~-~~specific post~~-~~translational modifications to histones generates an epigenetic regulatory network that directs fundamental~~ DNA-~~mediated processes~~ and ~~governs key stages in development. Methylation~~ of ~~histone H4 lysine~~-20 ~~has been implicated in DNA repair, transcriptional silencing, genomic stability and regulation~~ of ~~replication. We present the structure of the histone H4K20 methyltransferase Suv4-20h2 in complex with its~~ histone H4 ~~peptide substrate and S-adenosyl methionine cofactor~~. ~~Analysis of the structure reveals that the Suv4-20h2 active site diverges from the canonical SET domain configuration and generates~~ a ~~high degree of both substrate and product specificity. Together with supporting biochemical data comparing Suv4-20h1 and Suv4-20h2, we demonstrate that~~ the ~~Suv4~~-~~20 family enzymes take a previously mono-methylated H4K20 substrate~~ and ~~generate an exclusively di-methylated product. We therefore predict that other enzymes are responsible for the tri-methylation~~ of ~~histone H4K20 that marks silenced heterochromatin.~~	+
-		+
-	~~A novel route to product specificity in the Suv4~~-20 ~~family~~ of histone ~~H4K20 methyltransferases.,Southall SM, Cronin NB, Wilson JR Nucleic Acids Res. 2013 Sep 18~~. PMID:~~24049080~~<ref>PMID:~~24049080~~</ref>	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 4bup" style="background-color~~:~~#fffaf0;"~~></~~div~~>	+

	==See Also==		==See Also==
-	*[[Histone methyltransferase\|Histone methyltransferase]]	+	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Histone-lysine N-methyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Cronin~~, N B~~]]	+	[[Category: Cronin NB]]
-	[[Category: Southall~~, S M~~]]	+	[[Category: Southall SM]]
-	[[Category: Wilson~~, J R]]~~	+	[[Category: Wilson JR]]
-	~~[[Category: Epigenetic]]~~	+
-	~~[[Category: Histone]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:19, 13 March 2019

OCA — Wed, 13 Mar 2019 13:19:49 GMT

←Older revision		Revision as of 13:19, 13 March 2019
Line 1:		Line 1:

	==A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases==		==A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases==
-	<StructureSection load='4bup' size='340' side='right' caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>	+	<StructureSection load='4bup' size='340' side='right'caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BUP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BUP FirstGlance]. <br>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Histone-lysine N-methyltransferase]]		[[Category: Histone-lysine N-methyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Cronin, N B]]		[[Category: Cronin, N B]]

OCA at 23:07, 4 August 2016

OCA — Thu, 04 Aug 2016 23:07:44 GMT

←Older revision		Revision as of 23:07, 4 August 2016
Line 1:		Line 1:
		+
	==A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases==		==A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases==
	<StructureSection load='4bup' size='340' side='right' caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>		<StructureSection load='4bup' size='340' side='right' caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4au7\|4au7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4au7\|4au7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [http://www.ebi.ac.uk/pdbsum/4bup PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [http://pdbe.org/4bup PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [http://www.ebi.ac.uk/pdbsum/4bup PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bup ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bup" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:51, 25 December 2014

OCA — Thu, 25 Dec 2014 13:51:50 GMT

←Older revision		Revision as of 13:51, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bup\| PDB=4bup \| SCENE= }}~~	+	==A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases==
-	===A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases===	+	<StructureSection load='4bup' size='340' side='right' caption='[[4bup]], [[Resolution\|resolution]] 2.17Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24049080}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BUP FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4au7\|4au7]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bup OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bup RCSB], [http://www.ebi.ac.uk/pdbsum/4bup PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/SV421_MOUSE SV421_MOUSE]] Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).<ref>PMID:15145825</ref>		[[http://www.uniprot.org/uniprot/SV421_MOUSE SV421_MOUSE]] Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).<ref>PMID:15145825</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The delivery of site-specific post-translational modifications to histones generates an epigenetic regulatory network that directs fundamental DNA-mediated processes and governs key stages in development. Methylation of histone H4 lysine-20 has been implicated in DNA repair, transcriptional silencing, genomic stability and regulation of replication. We present the structure of the histone H4K20 methyltransferase Suv4-20h2 in complex with its histone H4 peptide substrate and S-adenosyl methionine cofactor. Analysis of the structure reveals that the Suv4-20h2 active site diverges from the canonical SET domain configuration and generates a high degree of both substrate and product specificity. Together with supporting biochemical data comparing Suv4-20h1 and Suv4-20h2, we demonstrate that the Suv4-20 family enzymes take a previously mono-methylated H4K20 substrate and generate an exclusively di-methylated product. We therefore predict that other enzymes are responsible for the tri-methylation of histone H4K20 that marks silenced heterochromatin.
		+
		+	A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases.,Southall SM, Cronin NB, Wilson JR Nucleic Acids Res. 2013 Sep 18. PMID:24049080<ref>PMID:24049080</ref>

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4bup]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:024049080</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Histone methyltransferase\|Histone methyltransferase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Histone-lysine N-methyltransferase]]		[[Category: Histone-lysine N-methyltransferase]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
-	[[Category: Cronin, N B.]]	+	[[Category: Cronin, N B]]
-	[[Category: Southall, S M.]]	+	[[Category: Southall, S M]]
-	[[Category: Wilson, J R.]]	+	[[Category: Wilson, J R]]
	[[Category: Epigenetic]]		[[Category: Epigenetic]]
	[[Category: Histone]]		[[Category: Histone]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:09, 15 January 2014

OCA — Wed, 15 Jan 2014 07:09:27 GMT

←Older revision		Revision as of 07:09, 15 January 2014
Line 2:		Line 2:
	===A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases===		===A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases===
	{{ABSTRACT_PUBMED_24049080}}		{{ABSTRACT_PUBMED_24049080}}
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/SV421_MOUSE SV421_MOUSE]] Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).<ref>PMID:15145825</ref>

	==About this Structure==		==About this Structure==
-	[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA].	+	[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024049080</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024049080</ref><references group="xtra"/><references/>
	[[Category: Histone-lysine N-methyltransferase]]		[[Category: Histone-lysine N-methyltransferase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Cronin, N B.]]		[[Category: Cronin, N B.]]
	[[Category: Southall, S M.]]		[[Category: Southall, S M.]]

OCA at 07:25, 2 October 2013

OCA — Wed, 02 Oct 2013 07:25:22 GMT

←Older revision		Revision as of 07:25, 2 October 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bup\| PDB=4bup \| SCENE= }}
		+	===A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases===
		+	{{ABSTRACT_PUBMED_24049080}}

-	~~The entry~~ 4bup is ~~ON HOLD~~	+	==About this Structure==
		+	[[4bup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BUP OCA].

-	~~Authors~~: ~~Southall, S.M.,~~ Cronin, N.B., Wilson, J.R.	+	==Reference==
-		+	<ref group="xtra">PMID:024049080</ref><references group="xtra"/><references/>
-	~~Description~~: ~~A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases~~	+	[[Category: Histone-lysine N-methyltransferase]]
		+	[[Category: Mus musculus]]
		+	[[Category: Cronin, N B.]]
		+	[[Category: Southall, S M.]]
		+	[[Category: Wilson, J R.]]
		+	[[Category: Epigenetic]]
		+	[[Category: Histone]]
		+	[[Category: Transferase]]

OCA: Protected "4bup" [edit=sysop:move=sysop]

OCA — Wed, 03 Jul 2013 12:52:59 GMT

Protected "4bup" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:52, 3 July 2013

OCA: New page: '''Unreleased structure''' The entry 4bup is ON HOLD Authors: Southall, S.M., Cronin, N.B., Wilson, J.R. Description: A novel route to product specificity in the Suv4-20 family of hist...

OCA — Wed, 03 Jul 2013 12:52:58 GMT

New page: '''Unreleased structure''' The entry 4bup is ON HOLD Authors: Southall, S.M., Cronin, N.B., Wilson, J.R. Description: A novel route to product specificity in the Suv4-20 family of hist...

New page

'''Unreleased structure'''

The entry 4bup is ON HOLD

Authors: Southall, S.M., Cronin, N.B., Wilson, J.R.

Description: A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases