4bxo - Revision history

OCA at 11:06, 9 May 2024

OCA — Thu, 09 May 2024 11:06:40 GMT

←Older revision		Revision as of 11:06, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [https://pdbe.org/4bxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [https://www.ebi.ac.uk/pdbsum/4bxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [https://pdbe.org/4bxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [https://www.ebi.ac.uk/pdbsum/4bxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxo ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8]	+	[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fanconi anemia (FA) is a disorder associated with a failure in DNA repair. FANCM (defective in FA complementation group M) and its partner FAAP24 target other FA proteins to sites of DNA damage. FANCM-FAAP24 is related to XPF/MUS81 endonucleases but lacks endonucleolytic activity. We report a structure of an FANCM C-terminal fragment (FANCMCTD) bound to FAAP24 and DNA. This S-shaped structure reveals the FANCM (HhH)2 domain is buried, whereas the FAAP24 (HhH)2 domain engages DNA. We identify a second DNA contact and a metal center within the FANCM pseudo-nuclease domain and demonstrate that mutations in either region impair double-stranded DNA binding in vitro and FANCM-FAAP24 function in vivo. We show the FANCM translocase domain lies in proximity to FANCMCTD by electron microscopy and that binding fork DNA structures stimulate its ATPase activity. This suggests a tracking model for FANCM-FAAP24 until an encounter with a stalled replication fork triggers ATPase-mediated fork remodeling.
		+
		+	Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex.,Coulthard R, Deans AJ, Swuec P, Bowles M, Costa A, West SC, McDonald NQ Structure. 2013 Aug 6. pii: S0969-2126(13)00255-4. doi:, 10.1016/j.str.2013.07.006. PMID:23932590<ref>PMID:23932590</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bxo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:15, 7 September 2022

OCA — Wed, 07 Sep 2022 17:15:16 GMT

←Older revision		Revision as of 17:15, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4bxo' size='340' side='right'caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4bxo' size='340' side='right'caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BXO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [https://pdbe.org/4bxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [https://www.ebi.ac.uk/pdbsum/4bxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxo ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [~~http~~://pdbe.org/4bxo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bxo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxo ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.	+	[[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8] [[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.	+	[[https://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Fanconi anemia (FA) is a disorder associated with a failure in DNA repair. FANCM (defective in FA complementation group M) and its partner FAAP24 target other FA proteins to sites of DNA damage. FANCM-FAAP24 is related to XPF/MUS81 endonucleases but lacks endonucleolytic activity. We report a structure of an FANCM C-terminal fragment (FANCMCTD) bound to FAAP24 and DNA. This S-shaped structure reveals the FANCM (HhH)2 domain is buried, whereas the FAAP24 (HhH)2 domain engages DNA. We identify a second DNA contact and a metal center within the FANCM pseudo-nuclease domain and demonstrate that mutations in either region impair double-stranded DNA binding in vitro and FANCM-FAAP24 function in vivo. We show the FANCM translocase domain lies in proximity to FANCMCTD by electron microscopy and that binding fork DNA structures stimulate its ATPase activity. This suggests a tracking model for FANCM-FAAP24 until an encounter with a stalled replication fork triggers ATPase-mediated fork remodeling.	+
-		+
-	Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex.,Coulthard R, Deans AJ, Swuec P, Bowles M, Costa A, West SC, McDonald NQ Structure. 2013 Aug 6. pii: S0969-2126(13)00255-4. doi:, 10.1016/j.str.2013.07.006. PMID:23932590<ref>PMID:23932590</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4bxo" style="background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RNA helicase~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Bowles, M]]	+	[[Category: Bowles M]]
-	[[Category: Costa, A]]	+	[[Category: Costa A]]
-	[[Category: Coulthard, R]]	+	[[Category: Coulthard R]]
-	[[Category: Deans, A]]	+	[[Category: Deans A]]
-	[[Category: McDonald, N]]	+	[[Category: McDonald N]]
-	[[Category: Purkiss, A]]	+	[[Category: Purkiss A]]
-	[[Category: Swuec, P]]	+	[[Category: Swuec P]]
-	[[Category: West, S]]	+	[[Category: West S]]
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Pseudo-nuclease~~]]	+

OCA at 17:45, 14 August 2019

OCA — Wed, 14 Aug 2019 17:45:31 GMT

←Older revision		Revision as of 17:45, 14 August 2019
Line 1:		Line 1:

	==Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex==		==Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex==
-	<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4bxo' size='340' side='right'caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Bowles, M]]		[[Category: Bowles, M]]

OCA at 08:16, 3 August 2017

OCA — Thu, 03 Aug 2017 08:16:44 GMT

←Older revision		Revision as of 08:16, 3 August 2017
Line 3:		Line 3:
	<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Bowles, M]]		[[Category: Bowles, M]]

OCA at 00:01, 5 August 2016

OCA — Fri, 05 Aug 2016 00:01:54 GMT

←Older revision		Revision as of 00:01, 5 August 2016
Line 1:		Line 1:
		+
	==Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex==		==Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex==
	<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [http://www.ebi.ac.uk/pdbsum/4bxo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [http://pdbe.org/4bxo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [http://www.ebi.ac.uk/pdbsum/4bxo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxo ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bxo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Bowles, M]]		[[Category: Bowles, M]]

OCA at 08:55, 21 December 2014

OCA — Sun, 21 Dec 2014 08:55:29 GMT

←Older revision		Revision as of 08:55, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bxo\| PDB=4bxo \| SCENE= }}~~	+	==Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex==
-	===Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex===	+	<StructureSection load='4bxo' size='340' side='right' caption='[[4bxo]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23932590}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4bxo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXO FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bxo RCSB], [http://www.ebi.ac.uk/pdbsum/4bxo PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.		[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.
-		+	== Function ==
-	==Function==	+
	[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8] [[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.		[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8] [[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fanconi anemia (FA) is a disorder associated with a failure in DNA repair. FANCM (defective in FA complementation group M) and its partner FAAP24 target other FA proteins to sites of DNA damage. FANCM-FAAP24 is related to XPF/MUS81 endonucleases but lacks endonucleolytic activity. We report a structure of an FANCM C-terminal fragment (FANCMCTD) bound to FAAP24 and DNA. This S-shaped structure reveals the FANCM (HhH)2 domain is buried, whereas the FAAP24 (HhH)2 domain engages DNA. We identify a second DNA contact and a metal center within the FANCM pseudo-nuclease domain and demonstrate that mutations in either region impair double-stranded DNA binding in vitro and FANCM-FAAP24 function in vivo. We show the FANCM translocase domain lies in proximity to FANCMCTD by electron microscopy and that binding fork DNA structures stimulate its ATPase activity. This suggests a tracking model for FANCM-FAAP24 until an encounter with a stalled replication fork triggers ATPase-mediated fork remodeling.

-	~~==About this~~ Structure==	+	Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex.,Coulthard R, Deans AJ, Swuec P, Bowles M, Costa A, West SC, McDonald NQ Structure. 2013 Aug 6. pii: S0969-2126(13)00255-4. doi:, 10.1016/j.str.2013.07.006. PMID:23932590<ref>PMID:23932590</ref>
-	~~[[4bxo]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4BXO OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023932590</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
-	[[Category: Bowles, M.]]	+	[[Category: Bowles, M]]
-	[[Category: Costa, A.]]	+	[[Category: Costa, A]]
-	[[Category: Coulthard, R.]]	+	[[Category: Coulthard, R]]
-	[[Category: Deans, A.]]	+	[[Category: Deans, A]]
-	[[Category: McDonald, N.]]	+	[[Category: McDonald, N]]
-	[[Category: Purkiss, A.]]	+	[[Category: Purkiss, A]]
-	[[Category: Swuec, P.]]	+	[[Category: Swuec, P]]
-	[[Category: West, S.]]	+	[[Category: West, S]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Pseudo-nuclease]]		[[Category: Pseudo-nuclease]]

OCA at 07:29, 28 August 2013

OCA — Wed, 28 Aug 2013 07:29:52 GMT

←Older revision		Revision as of 07:29, 28 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bxo\| PDB=4bxo \| SCENE= }}
		+	===Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex===
		+	{{ABSTRACT_PUBMED_23932590}}

-	The ~~entry 4bxo~~ is ~~ON HOLD until Paper Publication~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.

-	~~Authors~~: ~~Coulthard, R~~.~~, Deans, A~~., ~~Swuec, P~~.~~, Bowles, M~~., ~~Purkiss, A~~.~~, Costa, A~~., ~~West, S~~., ~~McDonald, N~~.	+	==Function==
		+	[[http://www.uniprot.org/uniprot/FANCM_HUMAN FANCM_HUMAN]] ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.<ref>PMID:16116422</ref> <ref>PMID:16116434</ref> [REFERENCE:7][REFERENCE:8] [[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.

-	~~Description~~: ~~Architecture and DNA recognition elements of the Fanconi anemia FANCM~~-~~FAAP24~~ complex	+	==About this Structure==
		+	[[4bxo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023932590</ref><references group="xtra"/><references/>
		+	[[Category: Homo sapiens]]
		+	[[Category: RNA helicase]]
		+	[[Category: Bowles, M.]]
		+	[[Category: Costa, A.]]
		+	[[Category: Coulthard, R.]]
		+	[[Category: Deans, A.]]
		+	[[Category: McDonald, N.]]
		+	[[Category: Purkiss, A.]]
		+	[[Category: Swuec, P.]]
		+	[[Category: West, S.]]
		+	[[Category: Dna binding]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Pseudo-nuclease]]

OCA: Protected "4bxo" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2013 12:03:07 GMT

Protected "4bxo" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:03, 24 July 2013

OCA: New page: '''Unreleased structure''' The entry 4bxo is ON HOLD until Paper Publication Authors: Coulthard, R., Deans, A., Swuec, P., Bowles, M., Purkiss, A., Costa, A., West, S., McDonald, N. De...

OCA — Wed, 24 Jul 2013 12:03:06 GMT

New page: '''Unreleased structure''' The entry 4bxo is ON HOLD until Paper Publication Authors: Coulthard, R., Deans, A., Swuec, P., Bowles, M., Purkiss, A., Costa, A., West, S., McDonald, N. De...

New page

'''Unreleased structure'''

The entry 4bxo is ON HOLD until Paper Publication

Authors: Coulthard, R., Deans, A., Swuec, P., Bowles, M., Purkiss, A., Costa, A., West, S., McDonald, N.

Description: Architecture and DNA recognition elements of the Fanconi anemia FANCM-FAAP24 complex