4bxp - Revision history

OCA at 11:06, 9 May 2024

OCA — Thu, 09 May 2024 11:06:40 GMT

←Older revision		Revision as of 11:06, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxp OCA], [https://pdbe.org/4bxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxp RCSB], [https://www.ebi.ac.uk/pdbsum/4bxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxp OCA], [https://pdbe.org/4bxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxp RCSB], [https://www.ebi.ac.uk/pdbsum/4bxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/E7FCY1_DANRE E7FCY1_DANRE]]	+	[https://www.uniprot.org/uniprot/E7FCY1_DANRE E7FCY1_DANRE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Centrioles organise centrosomes and template cilia and flagella. Several centriole and centrosome proteins have been linked to microcephaly (MCPH), a neuro-developmental disease associated with small brain size. CPAP (MCPH6) and STIL (MCPH7) are required for centriole assembly, but it is unclear how mutations in them lead to microcephaly. We show that the TCP domain of CPAP constitutes a novel proline recognition domain that forms a 1:1 complex with a short, highly conserved target motif in STIL. Crystal structures of this complex reveal an unusual, all-beta structure adopted by the TCP domain and explain how a microcephaly mutation in CPAP compromises complex formation. Through point mutations, we demonstrate that complex formation is essential for centriole duplication in vivo. Our studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis. DOI:http://dx.doi.org/10.7554/eLife.01071.001.
		+
		+	Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly.,Cottee MA, Muschalik N, Wong YL, Johnson CM, Johnson S, Andreeva A, Oegema K, Lea SM, Raff JW, van Breugel M Elife. 2013 Sep 17;2:e01071. doi: 10.7554/eLife.01071. PMID:24052813<ref>PMID:24052813</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bxp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:15, 7 September 2022

OCA — Wed, 07 Sep 2022 17:15:19 GMT

←Older revision		Revision as of 17:15, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4bxp' size='340' side='right'caption='[[4bxp]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4bxp' size='340' side='right'caption='[[4bxp]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brachidanio_rerio Brachidanio~~ rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BXP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXP FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bxq\|4bxq]], [[4bxr\|4bxr]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxp OCA], [https://pdbe.org/4bxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxp RCSB], [https://www.ebi.ac.uk/pdbsum/4bxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxp ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxp OCA], [~~http~~://pdbe.org/4bxp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bxp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bxp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxp ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/E7FCY1_DANRE E7FCY1_DANRE]]
-	Centrioles organise centrosomes and template cilia and flagella. Several centriole and centrosome proteins have been linked to microcephaly (MCPH), a neuro-developmental disease associated with small brain size. CPAP (MCPH6) and STIL (MCPH7) are required for centriole assembly, but it is unclear how mutations in them lead to microcephaly. We show that the TCP domain of CPAP constitutes a novel proline recognition domain that forms a 1:1 complex with a short, highly conserved target motif in STIL. Crystal structures of this complex reveal an unusual, all-beta structure adopted by the TCP domain and explain how a microcephaly mutation in CPAP compromises complex formation. Through point mutations, we demonstrate that complex formation is essential for centriole duplication in vivo. Our studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis. DOI:http://dx.~~doi~~.org/~~10.7554/eLife.01071.001.~~	+
-		+
-	Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly.,Cottee MA, Muschalik N, Wong YL, Johnson CM, Johnson S, Andreeva A, Oegema K, Lea SM, Raff JW, van Breugel M Elife. 2013 Sep 17;2:e01071. doi: 10.7554/eLife.01071. PMID:24052813<ref>PMID:24052813</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4bxp" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brachidanio~~ rerio]]	+	[[Category: Danio rerio]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Breugel, M ~~van]]~~	+	[[Category: Van Breugel M]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Centriole duplication~~]]	+

OCA at 16:37, 29 January 2020

OCA — Wed, 29 Jan 2020 16:37:52 GMT

←Older revision		Revision as of 16:37, 29 January 2020
Line 1:		Line 1:

	==Structure of the wild-type TCP10 domain of Danio rerio CPAP==		==Structure of the wild-type TCP10 domain of Danio rerio CPAP==
-	<StructureSection load='4bxp' size='340' side='right' caption='[[4bxp]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4bxp' size='340' side='right'caption='[[4bxp]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXP FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Brachidanio rerio]]		[[Category: Brachidanio rerio]]
		+	[[Category: Large Structures]]
	[[Category: Breugel, M van]]		[[Category: Breugel, M van]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Centriole duplication]]		[[Category: Centriole duplication]]

OCA at 02:38, 5 August 2016

OCA — Fri, 05 Aug 2016 02:38:39 GMT

←Older revision		Revision as of 02:38, 5 August 2016
Line 1:		Line 1:
-	{{STRUCTURE_4bxp\| PDB=4bxp \| SCENE= }}
-	===Structure of the wild-type TCP10 domain of Danio rerio CPAP===
-	{{ABSTRACT_PUBMED_24052813}}

-	==~~About this~~ Structure==	+	==Structure of the wild-type TCP10 domain of Danio rerio CPAP==
-	[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Danio_rerio Danio~~ rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA].	+	<StructureSection load='4bxp' size='340' side='right' caption='[[4bxp]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXP FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bxq\|4bxq]], [[4bxr\|4bxr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxp OCA], [http://pdbe.org/4bxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bxp RCSB], [http://www.ebi.ac.uk/pdbsum/4bxp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxp ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Centrioles organise centrosomes and template cilia and flagella. Several centriole and centrosome proteins have been linked to microcephaly (MCPH), a neuro-developmental disease associated with small brain size. CPAP (MCPH6) and STIL (MCPH7) are required for centriole assembly, but it is unclear how mutations in them lead to microcephaly. We show that the TCP domain of CPAP constitutes a novel proline recognition domain that forms a 1:1 complex with a short, highly conserved target motif in STIL. Crystal structures of this complex reveal an unusual, all-beta structure adopted by the TCP domain and explain how a microcephaly mutation in CPAP compromises complex formation. Through point mutations, we demonstrate that complex formation is essential for centriole duplication in vivo. Our studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis. DOI:http://dx.doi.org/10.7554/eLife.01071.001.

-	~~==Reference==~~	+	Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly.,Cottee MA, Muschalik N, Wong YL, Johnson CM, Johnson S, Andreeva A, Oegema K, Lea SM, Raff JW, van Breugel M Elife. 2013 Sep 17;2:e01071. doi: 10.7554/eLife.01071. PMID:24052813<ref>PMID:24052813</ref>
-	<ref ~~group="xtra"~~>PMID:~~024052813~~</ref><~~references group~~="~~xtra~~"/><references/>	+
-	[[Category: ~~Danio~~ rerio]]	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: Breugel, M van.]]	+	</div>
		+	<div class="pdbe-citations 4bxp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brachidanio rerio]]
		+	[[Category: Breugel, M van]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Centriole duplication]]		[[Category: Centriole duplication]]

OCA at 06:00, 2 October 2013

OCA — Wed, 02 Oct 2013 06:00:27 GMT

←Older revision		Revision as of 06:00, 2 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4bxp\| PDB=4bxp \| SCENE= }}		{{STRUCTURE_4bxp\| PDB=4bxp \| SCENE= }}
	===Structure of the wild-type TCP10 domain of Danio rerio CPAP===		===Structure of the wild-type TCP10 domain of Danio rerio CPAP===
		+	{{ABSTRACT_PUBMED_24052813}}

	==About this Structure==		==About this Structure==
	[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA].		[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024052813</ref><references group="xtra"/><references/>
	[[Category: Danio rerio]]		[[Category: Danio rerio]]
	[[Category: Breugel, M van.]]		[[Category: Breugel, M van.]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Centriole duplication]]		[[Category: Centriole duplication]]

OCA at 08:16, 29 September 2013

OCA — Sun, 29 Sep 2013 08:16:04 GMT

←Older revision		Revision as of 08:16, 29 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bxp\| PDB=4bxp \| SCENE= }}
		+	===Structure of the wild-type TCP10 domain of Danio rerio CPAP===

-	~~The entry~~ 4bxp is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4bxp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXP OCA].
-	~~Authors~~: ~~van~~ Breugel, M.	+	[[Category: Danio rerio]]
-		+	[[Category: Breugel, M van.]]
-	~~Description~~: ~~Structure of the wild-type TCP10 domain of Danio rerio CPAP~~	+	[[Category: Cell cycle]]
		+	[[Category: Centriole duplication]]

OCA: Protected "4bxp" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2013 12:03:10 GMT

Protected "4bxp" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:03, 24 July 2013

OCA: New page: '''Unreleased structure''' The entry 4bxp is ON HOLD until Paper Publication Authors: van Breugel, M. Description: Structure of the wild-type TCP10 domain of Danio rerio CPAP

OCA — Wed, 24 Jul 2013 12:03:09 GMT

New page: '''Unreleased structure''' The entry 4bxp is ON HOLD until Paper Publication Authors: van Breugel, M. Description: Structure of the wild-type TCP10 domain of Danio rerio CPAP

New page

'''Unreleased structure'''

The entry 4bxp is ON HOLD until Paper Publication

Authors: van Breugel, M.

Description: Structure of the wild-type TCP10 domain of Danio rerio CPAP