4by6 - Revision history

OCA at 11:06, 9 May 2024

OCA — Thu, 09 May 2024 11:06:52 GMT

←Older revision		Revision as of 11:06, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_CEN.PK113-7D Saccharomyces cerevisiae CEN.PK113-7D] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_CEN.PK113-7D Saccharomyces cerevisiae CEN.PK113-7D] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.797Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [https://pdbe.org/4by6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [https://www.ebi.ac.uk/pdbsum/4by6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4by6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [https://pdbe.org/4by6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [https://www.ebi.ac.uk/pdbsum/4by6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4by6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>	+	[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-A-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.
		+
		+	Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.,Bhaskar V, Roudko V, Basquin J, Sharma K, Urlaub H, Seraphin B, Conti E Nat Struct Mol Biol. 2013 Oct 13. doi: 10.1038/nsmb.2686. PMID:24121231<ref>PMID:24121231</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4by6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:16, 7 September 2022

OCA — Wed, 07 Sep 2022 17:16:08 GMT

←Older revision		Revision as of 17:16, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4by6' size='340' side='right'caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4by6' size='340' side='right'caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_CEN.PK113-7D Saccharomyces cerevisiae CEN.PK113-7D] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [~~http~~://pdbe.org/4by6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4by6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4by6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [https://pdbe.org/4by6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [https://www.ebi.ac.uk/pdbsum/4by6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4by6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/~~NOT5_YEAST NOT5_YEAST~~]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[http://www.uniprot.org/uniprot/NOT2_YEAST NOT2_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>	+	[[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-A-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.	+
-		+
-	Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.,Bhaskar V, Roudko V, Basquin J, Sharma K, Urlaub H, Seraphin B, Conti E Nat Struct Mol Biol. 2013 Oct 13. doi: 10.1038/nsmb.2686. PMID:24121231<ref>PMID:24121231</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4by6" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Basquin, J~~]]	+	[[Category: Saccharomyces cerevisiae CEN PK113-7D]]
-	[[Category: ~~Bhaskar, V~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Conti, E~~]]	+	[[Category: Basquin J]]
-	[[Category: ~~Cterminal components of the ccr4_not complex~~]]	+	[[Category: Bhaskar V]]
-	[[Category: ~~Transcription~~]]	+	[[Category: Conti E]]

OCA at 13:46, 13 March 2019

OCA — Wed, 13 Mar 2019 13:46:12 GMT

←Older revision		Revision as of 13:46, 13 March 2019
Line 1:		Line 1:

	==Yeast Not1-Not2-Not5 complex==		==Yeast Not1-Not2-Not5 complex==
-	<StructureSection load='4by6' size='340' side='right' caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4by6' size='340' side='right'caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Basquin, J]]		[[Category: Basquin, J]]
	[[Category: Bhaskar, V]]		[[Category: Bhaskar, V]]

OCA at 19:49, 4 August 2016

OCA — Thu, 04 Aug 2016 19:49:00 GMT

←Older revision		Revision as of 19:49, 4 August 2016
Line 1:		Line 1:
		+
	==Yeast Not1-Not2-Not5 complex==		==Yeast Not1-Not2-Not5 complex==
	<StructureSection load='4by6' size='340' side='right' caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4by6' size='340' side='right' caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [http://www.ebi.ac.uk/pdbsum/4by6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [http://pdbe.org/4by6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [http://www.ebi.ac.uk/pdbsum/4by6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4by6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4by6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:33, 25 December 2014

OCA — Thu, 25 Dec 2014 12:33:44 GMT

←Older revision		Revision as of 12:33, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4by6\| PDB~~=4by6 \| ~~SCENE~~= }}	+	==Yeast Not1-Not2-Not5 complex==
-	===~~Yeast Not1~~-~~Not2~~-~~Not5 complex~~===	+	<StructureSection load='4by6' size='340' side='right' caption='[[4by6]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24121231}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BY6 FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4by6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4by6 RCSB], [http://www.ebi.ac.uk/pdbsum/4by6 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/NOT5_YEAST NOT5_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[http://www.uniprot.org/uniprot/NOT2_YEAST NOT2_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>		[[http://www.uniprot.org/uniprot/NOT5_YEAST NOT5_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[http://www.uniprot.org/uniprot/NOT2_YEAST NOT2_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-A-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.

-	~~==About this~~ Structure==	+	Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.,Bhaskar V, Roudko V, Basquin J, Sharma K, Urlaub H, Seraphin B, Conti E Nat Struct Mol Biol. 2013 Oct 13. doi: 10.1038/nsmb.2686. PMID:24121231<ref>PMID:24121231</ref>
-	~~[[4by6]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Baker's_yeast Baker's yeast]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4BY6 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024121231</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
-	[[Category: Basquin, J.]]	+	[[Category: Basquin, J]]
-	[[Category: Bhaskar, V.]]	+	[[Category: Bhaskar, V]]
-	[[Category: Conti, E.]]	+	[[Category: Conti, E]]
	[[Category: Cterminal components of the ccr4_not complex]]		[[Category: Cterminal components of the ccr4_not complex]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 12:02, 20 November 2013

OCA — Wed, 20 Nov 2013 12:02:11 GMT

←Older revision		Revision as of 12:02, 20 November 2013
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_cen.pk113-7d Saccharomyces cerevisiae cen.pk113-7d] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA].	+	[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024121231</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024121231</ref><references group="xtra"/><references/>
-	[[Category: ~~Saccharomyces cerevisiae cen pk113-7d]]~~	+	[[Category: Baker's yeast]]
-	~~[[Category: Saccharomyces cerevisiae s288c~~]]	+
	[[Category: Basquin, J.]]		[[Category: Basquin, J.]]
	[[Category: Bhaskar, V.]]		[[Category: Bhaskar, V.]]

OCA at 05:02, 23 October 2013

OCA — Wed, 23 Oct 2013 05:02:22 GMT

←Older revision		Revision as of 05:02, 23 October 2013
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	{{STRUCTURE_4by6\| PDB=4by6 \| SCENE= }}		{{STRUCTURE_4by6\| PDB=4by6 \| SCENE= }}
	===Yeast Not1-Not2-Not5 complex===		===Yeast Not1-Not2-Not5 complex===
		+	{{ABSTRACT_PUBMED_24121231}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024121231</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae cen pk113-7d]]		[[Category: Saccharomyces cerevisiae cen pk113-7d]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]

OCA at 08:05, 16 October 2013

OCA — Wed, 16 Oct 2013 08:05:54 GMT

←Older revision		Revision as of 08:05, 16 October 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4by6\| PDB=4by6 \| SCENE= }}
		+	===Yeast Not1-Not2-Not5 complex===

-	The ~~entry 4by6~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/NOT5_YEAST NOT5_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[http://www.uniprot.org/uniprot/NOT2_YEAST NOT2_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>

-	~~Authors~~: ~~Bhaskar, V~~.~~, Basquin, J~~.~~, Conti, E~~.	+	==About this Structure==
		+	[[4by6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_cen.pk113-7d Saccharomyces cerevisiae cen.pk113-7d] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY6 OCA].

-	~~Description~~: ~~Yeast Not1~~-~~Not2-Not5~~ complex	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Saccharomyces cerevisiae cen pk113-7d]]
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Basquin, J.]]
		+	[[Category: Bhaskar, V.]]
		+	[[Category: Conti, E.]]
		+	[[Category: Cterminal components of the ccr4_not complex]]
		+	[[Category: Transcription]]

OCA at 19:42, 7 August 2013

OCA — Wed, 07 Aug 2013 19:42:32 GMT

←Older revision		Revision as of 19:42, 7 August 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4by6 is ON HOLD	+	The entry 4by6 is ON HOLD until Paper Publication

	Authors: Bhaskar, V., Basquin, J., Conti, E.		Authors: Bhaskar, V., Basquin, J., Conti, E.

	Description: Yeast Not1-Not2-Not5 complex		Description: Yeast Not1-Not2-Not5 complex

OCA: Protected "4by6" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2013 12:03:57 GMT

Protected "4by6" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:03, 24 July 2013