4bz4 - Revision history

OCA at 17:17, 7 September 2022

OCA — Wed, 07 Sep 2022 17:17:01 GMT

←Older revision		Revision as of 17:17, 7 September 2022
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	<StructureSection load='4bz4' size='340' side='right'caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4bz4' size='340' side='right'caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Methylomicrobium_album_bg8~~ Methylomicrobium album ~~bg8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylomicrobium_album_BG8 Methylomicrobium album BG8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=~~P6G~~:~~HEXAETHYLENE~~+~~GLYCOL~~'>~~P6G~~</scene>, <scene name='pdbligand=~~PG4~~:~~TETRAETHYLENE~~+GLYCOL'>~~PG4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~KYN~~:~~(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC~~+~~ACID~~'>~~KYN~~</scene></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [https://pdbe.org/4bz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [https://www.ebi.ac.uk/pdbsum/4bz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bz4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [~~http~~://pdbe.org/4bz4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bz4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bz4 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/H8GPE3_METAL H8GPE3_METAL]]
-	CorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per protein molecule, which, based on X-ray Absorption Near Edge Structure analysis, is in the reduced state (Cu(I)). The structure of endogenously expressed CorA was solved using X-ray crystallography. The 1.6 A three-dimensional structure confirmed the binding of copper and revealed that the copper atom was coordinated in a mononuclear binding site defined by two histidines, one water molecule, and the tryptophan metabolite, kynurenine. This arrangement of the copper-binding site is similar to that of its homologous protein MopE* from Metylococcus capsulatus Bath, confirming the importance of kynurenine for copper binding in these proteins. Our findings show that CorA has an overall fold similar to MopE, including the unique copper(I)-binding site and most of the secondary structure elements. We suggest that CorA plays a role in the M. album BG8 copper acquisition.	+
-		+
-	CorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8.,Johnson KA, Ve T, Larsen O, Pedersen RB, Lillehaug JR, Jensen HB, Helland R, Karlsen OA PLoS One. 2014 Feb 3;9(2):~~e87750. doi: 10.1371~~/~~journal.pone.0087750. eCollection , 2014. PMID:24498370<ref>PMID:24498370<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4bz4" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Methylomicrobium album ~~bg8~~]]	+	[[Category: Methylomicrobium album BG8]]
-	[[Category: Helland, R]]	+	[[Category: Helland R]]
-	[[Category: Jensen~~, H B~~]]	+	[[Category: Jensen HB]]
-	[[Category: Johnson~~, K A~~]]	+	[[Category: Johnson KA]]
-	[[Category: Karlsen~~, O A~~]]	+	[[Category: Karlsen OA]]
-	[[Category: Lillehaug~~, J R~~]]	+	[[Category: Lillehaug JR]]
-	[[Category: Pedersen~~, R B~~]]	+	[[Category: Pedersen RB]]
-	[[Category: Ve, T]]	+	[[Category: Ve T]]
-	~~[[Category: Copper acquisition]]~~	+
-	~~[[Category: Copper-binding protein]]~~	+
-	~~[[Category: Methanotroph~~]]	+

OCA at 13:41, 13 March 2019

OCA — Wed, 13 Mar 2019 13:41:32 GMT

←Older revision		Revision as of 13:41, 13 March 2019
Line 1:		Line 1:

	==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition==		==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition==
-	<StructureSection load='4bz4' size='340' side='right' caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4bz4' size='340' side='right'caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8 Methylomicrobium album bg8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8 Methylomicrobium album bg8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Methylomicrobium album bg8]]		[[Category: Methylomicrobium album bg8]]
	[[Category: Helland, R]]		[[Category: Helland, R]]

OCA at 18:18, 11 August 2016

OCA — Thu, 11 Aug 2016 18:18:37 GMT

←Older revision		Revision as of 18:18, 11 August 2016
Line 1:		Line 1:
		+
	==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition==		==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition==
	<StructureSection load='4bz4' size='340' side='right' caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4bz4' size='340' side='right' caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bz4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [http://pdbe.org/4bz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bz4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bz4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:00, 5 January 2015

OCA — Mon, 05 Jan 2015 13:00:42 GMT

←Older revision		Revision as of 13:00, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4bz4\| PDB=4bz4 \| SCENE= }}~~	+	==CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition==
-	===CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition===	+	<StructureSection load='4bz4' size='340' side='right' caption='[[4bz4]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24498370}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8 Methylomicrobium album bg8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZ4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bz4 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per protein molecule, which, based on X-ray Absorption Near Edge Structure analysis, is in the reduced state (Cu(I)). The structure of endogenously expressed CorA was solved using X-ray crystallography. The 1.6 A three-dimensional structure confirmed the binding of copper and revealed that the copper atom was coordinated in a mononuclear binding site defined by two histidines, one water molecule, and the tryptophan metabolite, kynurenine. This arrangement of the copper-binding site is similar to that of its homologous protein MopE* from Metylococcus capsulatus Bath, confirming the importance of kynurenine for copper binding in these proteins. Our findings show that CorA has an overall fold similar to MopE, including the unique copper(I)-binding site and most of the secondary structure elements. We suggest that CorA plays a role in the M. album BG8 copper acquisition.

-	~~==About this Structure==~~	+	CorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8.,Johnson KA, Ve T, Larsen O, Pedersen RB, Lillehaug JR, Jensen HB, Helland R, Karlsen OA PLoS One. 2014 Feb 3;9(2):e87750. doi: 10.1371/journal.pone.0087750. eCollection , 2014. PMID:24498370<ref>PMID:24498370</ref>
-	~~[[4bz4]] is~~ a ~~6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8~~ Methylomicrobium album ~~bg8]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4BZ4 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024498370</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Methylomicrobium album bg8]]		[[Category: Methylomicrobium album bg8]]
-	[[Category: Helland, R.]]	+	[[Category: Helland, R]]
-	[[Category: Jensen, H B.]]	+	[[Category: Jensen, H B]]
-	[[Category: Johnson, K A.]]	+	[[Category: Johnson, K A]]
-	[[Category: Karlsen, O A.]]	+	[[Category: Karlsen, O A]]
-	[[Category: Lillehaug, J R.]]	+	[[Category: Lillehaug, J R]]
-	[[Category: Pedersen, R B.]]	+	[[Category: Pedersen, R B]]
-	[[Category: Ve, T.]]	+	[[Category: Ve, T]]
	[[Category: Copper acquisition]]		[[Category: Copper acquisition]]
	[[Category: Copper-binding protein]]		[[Category: Copper-binding protein]]
	[[Category: Methanotroph]]		[[Category: Methanotroph]]

OCA at 17:35, 19 February 2014

OCA — Wed, 19 Feb 2014 17:35:12 GMT

←Older revision		Revision as of 17:35, 19 February 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bz4\| PDB=4bz4 \| SCENE= }}
		+	===CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition===
		+	{{ABSTRACT_PUBMED_24498370}}

-	~~The entry~~ 4bz4 is ~~ON HOLD until Jul 24 2015~~	+	==About this Structure==
		+	[[4bz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylomicrobium_album_bg8 Methylomicrobium album bg8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ4 OCA].

-	~~Authors~~: ~~Johnson~~, ~~K.A~~., ~~Ve, T~~., ~~Pedersen, R.B~~., Lillehaug, J.R., ~~Jensen, H.~~B., ~~Helland, R., Karlsen, O.A~~.	+	==Reference==
-		+	<ref group="xtra">PMID:024498370</ref><references group="xtra"/><references/>
-	~~Description~~: ~~CorA is a surface-associated copper~~-binding protein ~~important in Methylomicrobium album BG8 copper acquisition~~	+	[[Category: Methylomicrobium album bg8]]
		+	[[Category: Helland, R.]]
		+	[[Category: Jensen, H B.]]
		+	[[Category: Johnson, K A.]]
		+	[[Category: Karlsen, O A.]]
		+	[[Category: Lillehaug, J R.]]
		+	[[Category: Pedersen, R B.]]
		+	[[Category: Ve, T.]]
		+	[[Category: Copper acquisition]]
		+	[[Category: Copper-binding protein]]
		+	[[Category: Methanotroph]]

OCA: Protected "4bz4" [edit=sysop:move=sysop]

OCA — Thu, 01 Aug 2013 04:00:32 GMT

Protected "4bz4" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:00, 1 August 2013

OCA: New page: '''Unreleased structure''' The entry 4bz4 is ON HOLD until Jul 24 2015 Authors: Johnson, K.A., Ve, T., Pedersen, R.B., Lillehaug, J.R., Jensen, H.B., Helland, R., Karlsen, O.A. Descrip...

OCA — Thu, 01 Aug 2013 04:00:31 GMT

New page: '''Unreleased structure''' The entry 4bz4 is ON HOLD until Jul 24 2015 Authors: Johnson, K.A., Ve, T., Pedersen, R.B., Lillehaug, J.R., Jensen, H.B., Helland, R., Karlsen, O.A. Descrip...

New page

'''Unreleased structure'''

The entry 4bz4 is ON HOLD until Jul 24 2015

Authors: Johnson, K.A., Ve, T., Pedersen, R.B., Lillehaug, J.R., Jensen, H.B., Helland, R., Karlsen, O.A.

Description: CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition