4bzk - Revision history

OCA at 11:07, 9 May 2024

OCA — Thu, 09 May 2024 11:07:10 GMT

←Older revision		Revision as of 11:07, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [https://pdbe.org/4bzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [https://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 40Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [https://pdbe.org/4bzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [https://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref>	+	[https://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.
		+
		+	The structure of the COPII transport-vesicle coat assembled on membranes.,Zanetti G, Prinz S, Daum S, Meister A, Schekman R, Bacia K, Briggs JA Elife. 2013 Sep 17;2:e00951. doi: 10.7554/eLife.00951. PMID:24062940<ref>PMID:24062940</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bzk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:17, 7 September 2022

OCA — Wed, 07 Sep 2022 17:17:38 GMT

←Older revision		Revision as of 17:17, 7 September 2022
Line 3:		Line 3:
	<SX load='4bzk' size='340' side='right' viewer='molstar' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>		<SX load='4bzk' size='340' side='right' viewer='molstar' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4BZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [https://pdbe.org/4bzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [https://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SEC31, WEB1, YDL195W, D1229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SEC13, ANU3, YLR208W, L8167.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [~~http~~://pdbe.org/4bzk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref> [[http://www.uniprot.org/uniprot/SEC13_YEAST SEC13_YEAST]] Functions as a component of the nuclear pore complex (NPC) and the COPII coat. It is one of 5 proteins constituting the COPII coat, which is involved in anterograde (ER to Golgi) double-membrane transport vesicle formation. First the small GTPase SAR1, activated by and binding to the integral ER membrane protein SEC12, exchanges GDP for GTP and recruits the heterodimer SEC23/24, which in turn recruits the heterotetramer SEC13-SEC31. The polymerization of COPII coat complexes then causes physically the deformation (budding) of the membrane, leading to the creation of a transport vesicle. The COPII complex is dissociated upon SAR1-GTP hydrolysis to SAR1-GDP. SEC23 functions as the SAR1 GTPase activating protein, whose activity is stimulated in the presence of SEC13/31. SEC13 is directly or indirectly required for normal ER membrane and nuclear envelope morphology. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution. Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.<ref>PMID:8565072</ref> <ref>PMID:6996832</ref> <ref>PMID:7026045</ref> <ref>PMID:2188733</ref> <ref>PMID:8548805</ref> <ref>PMID:8909535</ref> <ref>PMID:9409822</ref> <ref>PMID:9199164</ref> <ref>PMID:9427388</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:10747086</ref> <ref>PMID:11535824</ref> <ref>PMID:11717432</ref> <ref>PMID:12215173</ref> <ref>PMID:11823431</ref> <ref>PMID:12475940</ref> <ref>PMID:14627716</ref> <ref>PMID:21454883</ref>	+	[[https://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.	+
-		+
-	The structure of the COPII transport-vesicle coat assembled on membranes.,Zanetti G, Prinz S, Daum S, Meister A, Schekman R, Bacia K, Briggs JA Elife. 2013 Sep 17;2:e00951. doi: 10.7554/eLife.00951. PMID:24062940<ref>PMID:24062940</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4bzk" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bacia, K]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Briggs~~, J A.G~~]]	+	[[Category: Bacia K]]
-	[[Category: Daum, S]]	+	[[Category: Briggs JAG]]
-	[[Category: Meister, A]]	+	[[Category: Daum S]]
-	[[Category: Prinz, S]]	+	[[Category: Meister A]]
-	[[Category: Schekman, R]]	+	[[Category: Prinz S]]
-	[[Category: Zanetti, G]]	+	[[Category: Schekman R]]
-	~~[[Category: Protein transport]]~~	+	[[Category: Zanetti G]]
-	~~[[Category: Secretion]]~~	+
-	~~[[Category: Trafficking~~]]	+

OCA at 18:58, 10 April 2020

OCA — Fri, 10 Apr 2020 18:58:34 GMT

←Older revision		Revision as of 18:58, 10 April 2020
Line 3:		Line 3:
	<SX load='4bzk' size='340' side='right' viewer='molstar' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>		<SX load='4bzk' size='340' side='right' viewer='molstar' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SEC31, WEB1, YDL195W, D1229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SEC13, ANU3, YLR208W, L8167.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SEC31, WEB1, YDL195W, D1229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SEC13, ANU3, YLR208W, L8167.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://pdbe.org/4bzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://pdbe.org/4bzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:52, 6 March 2020

OCA — Fri, 06 Mar 2020 17:52:14 GMT

←Older revision		Revision as of 17:52, 6 March 2020
Line 1:		Line 1:

	==The structure of the COPII coat assembled on membranes==		==The structure of the COPII coat assembled on membranes==
-	<~~StructureSection~~ load='4bzk' size='340' side='right'caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>	+	<SX load='4bzk' size='340' side='right' viewer='molstar' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SEC31, WEB1, YDL195W, D1229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SEC13, ANU3, YLR208W, L8167.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://pdbe.org/4bzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://pdbe.org/4bzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 13:47, 13 March 2019

OCA — Wed, 13 Mar 2019 13:47:05 GMT

←Older revision		Revision as of 13:47, 13 March 2019
Line 1:		Line 1:

	==The structure of the COPII coat assembled on membranes==		==The structure of the COPII coat assembled on membranes==
-	<StructureSection load='4bzk' size='340' side='right' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>	+	<StructureSection load='4bzk' size='340' side='right'caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Bacia, K]]		[[Category: Bacia, K]]
	[[Category: Briggs, J A.G]]		[[Category: Briggs, J A.G]]

OCA at 11:46, 4 August 2016

OCA — Thu, 04 Aug 2016 11:46:23 GMT

←Older revision		Revision as of 11:46, 4 August 2016
Line 1:		Line 1:
		+
	==The structure of the COPII coat assembled on membranes==		==The structure of the COPII coat assembled on membranes==
	<StructureSection load='4bzk' size='340' side='right' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>		<StructureSection load='4bzk' size='340' side='right' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://pdbe.org/4bzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bzk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Bacia, K]]		[[Category: Bacia, K]]
	[[Category: Briggs, J A.G]]		[[Category: Briggs, J A.G]]

OCA at 08:59, 25 December 2014

OCA — Thu, 25 Dec 2014 08:59:16 GMT

←Older revision		Revision as of 08:59, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref> [[http://www.uniprot.org/uniprot/SEC13_YEAST SEC13_YEAST]] Functions as a component of the nuclear pore complex (NPC) and the COPII coat. It is one of 5 proteins constituting the COPII coat, which is involved in anterograde (ER to Golgi) double-membrane transport vesicle formation. First the small GTPase SAR1, activated by and binding to the integral ER membrane protein SEC12, exchanges GDP for GTP and recruits the heterodimer SEC23/24, which in turn recruits the heterotetramer SEC13-SEC31. The polymerization of COPII coat complexes then causes physically the deformation (budding) of the membrane, leading to the creation of a transport vesicle. The COPII complex is dissociated upon SAR1-GTP hydrolysis to SAR1-GDP. SEC23 functions as the SAR1 GTPase activating protein, whose activity is stimulated in the presence of SEC13/31. SEC13 is directly or indirectly required for normal ER membrane and nuclear envelope morphology. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution. Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.<ref>PMID:8565072</ref> <ref>PMID:6996832</ref> <ref>PMID:7026045</ref> <ref>PMID:2188733</ref> <ref>PMID:8548805</ref> <ref>PMID:8909535</ref> <ref>PMID:9409822</ref> <ref>PMID:9199164</ref> <ref>PMID:9427388</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:10747086</ref> <ref>PMID:11535824</ref> <ref>PMID:11717432</ref> <ref>PMID:12215173</ref> <ref>PMID:11823431</ref> <ref>PMID:12475940</ref> <ref>PMID:14627716</ref> <ref>PMID:21454883</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:56, 21 December 2014

OCA — Sun, 21 Dec 2014 09:56:38 GMT

←Older revision		Revision as of 09:56, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bzk\| PDB=4bzk \| SCENE= }}~~	+	==The structure of the COPII coat assembled on membranes==
-	===The structure of the COPII coat assembled on membranes===	+	<StructureSection load='4bzk' size='340' side='right' caption='[[4bzk]], [[Resolution\|resolution]] 40.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24062940}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZK FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bzi\|4bzi]], [[4bzj\|4bzj]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bzk RCSB], [http://www.ebi.ac.uk/pdbsum/4bzk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.

-	~~==Function==~~	+	The structure of the COPII transport-vesicle coat assembled on membranes.,Zanetti G, Prinz S, Daum S, Meister A, Schekman R, Bacia K, Briggs JA Elife. 2013 Sep 17;2:e00951. doi: 10.7554/eLife.00951. PMID:24062940<ref>PMID:24062940</ref>
-	~~[[http://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component~~ of the ~~coat protein complex II (~~COPII~~) which promotes the formation of~~ transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref> [[http://www.uniprot.org/uniprot/SEC13_YEAST SEC13_YEAST]] Functions as a component of the nuclear pore complex (NPC) and the COPII coat. It is one of 5 proteins constituting the COPII coat, which is involved in anterograde (ER to Golgi) double-~~membrane transport~~ vesicle ~~formation~~. ~~First the small GTPase SAR1~~, ~~activated by and binding to the integral ER membrane protein SEC12~~, ~~exchanges GDP for GTP and recruits the heterodimer SEC23/24~~, ~~which in turn recruits the heterotetramer SEC13-SEC31. The polymerization of COPII coat complexes then causes physically the deformation (budding) of the membrane~~, ~~leading to the creation of a transport vesicle. The COPII complex is dissociated upon SAR1-GTP hydrolysis to SAR1-GDP. SEC23 functions as the SAR1 GTPase activating protein~~, whose activity is stimulated in the presence of SEC13/31. SEC13 is directly or indirectly required for normal ER membrane and nuclear envelope morphology. It also functions as a component of the nuclear pore complex (NPC). NPC components, ~~collectively referred to as nucleoporins (NUPs)~~, ~~can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors~~. ~~SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution~~. ~~Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis~~.~~<ref>PMID:8565072<~~/~~ref> <ref>~~PMID:6996832</ref> <ref>PMID:7026045</ref> <ref>PMID:2188733</ref> <ref>PMID:8548805</ref> <ref>PMID:8909535</ref> <ref>PMID:9409822</ref> <ref>PMID:9199164</ref> <ref>PMID:9427388</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:10747086</ref> <ref>PMID:11535824</ref> <ref>PMID:11717432</ref> <ref>PMID:12215173</ref> <ref>PMID:11823431</ref> <ref>PMID:12475940</ref> <ref>PMID:14627716</ref> <ref>PMID:~~21454883~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4bzk]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024062940</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Bacia, K.]]	+	[[Category: Bacia, K]]
-	[[Category: Briggs, J A.G.]]	+	[[Category: Briggs, J A.G]]
-	[[Category: Daum, S.]]	+	[[Category: Daum, S]]
-	[[Category: Meister, A.]]	+	[[Category: Meister, A]]
-	[[Category: Prinz, S.]]	+	[[Category: Prinz, S]]
-	[[Category: Schekman, R.]]	+	[[Category: Schekman, R]]
-	[[Category: Zanetti, G.]]	+	[[Category: Zanetti, G]]
	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: Secretion]]		[[Category: Secretion]]
	[[Category: Trafficking]]		[[Category: Trafficking]]

OCA at 04:53, 10 October 2013

OCA — Thu, 10 Oct 2013 04:53:39 GMT

←Older revision		Revision as of 04:53, 10 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4bzk\| PDB=4bzk \| SCENE= }}		{{STRUCTURE_4bzk\| PDB=4bzk \| SCENE= }}
	===The structure of the COPII coat assembled on membranes===		===The structure of the COPII coat assembled on membranes===
		+	{{ABSTRACT_PUBMED_24062940}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024062940</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Bacia, K.]]		[[Category: Bacia, K.]]

OCA at 04:18, 20 September 2013

OCA — Fri, 20 Sep 2013 04:18:41 GMT

←Older revision		Revision as of 04:18, 20 September 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4bzk\| PDB=4bzk \| SCENE= }}
		+	===The structure of the COPII coat assembled on membranes===

-	The ~~entry 4bzk~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/SEC31_YEAST SEC31_YEAST]] Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.<ref>PMID:8548805</ref> <ref>PMID:8852839</ref> <ref>PMID:9190202</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:14627716</ref> [[http://www.uniprot.org/uniprot/SEC13_YEAST SEC13_YEAST]] Functions as a component of the nuclear pore complex (NPC) and the COPII coat. It is one of 5 proteins constituting the COPII coat, which is involved in anterograde (ER to Golgi) double-membrane transport vesicle formation. First the small GTPase SAR1, activated by and binding to the integral ER membrane protein SEC12, exchanges GDP for GTP and recruits the heterodimer SEC23/24, which in turn recruits the heterotetramer SEC13-SEC31. The polymerization of COPII coat complexes then causes physically the deformation (budding) of the membrane, leading to the creation of a transport vesicle. The COPII complex is dissociated upon SAR1-GTP hydrolysis to SAR1-GDP. SEC23 functions as the SAR1 GTPase activating protein, whose activity is stimulated in the presence of SEC13/31. SEC13 is directly or indirectly required for normal ER membrane and nuclear envelope morphology. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution. Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.<ref>PMID:8565072</ref> <ref>PMID:6996832</ref> <ref>PMID:7026045</ref> <ref>PMID:2188733</ref> <ref>PMID:8548805</ref> <ref>PMID:8909535</ref> <ref>PMID:9409822</ref> <ref>PMID:9199164</ref> <ref>PMID:9427388</ref> <ref>PMID:9023343</ref> <ref>PMID:10720463</ref> <ref>PMID:10747086</ref> <ref>PMID:11535824</ref> <ref>PMID:11717432</ref> <ref>PMID:12215173</ref> <ref>PMID:11823431</ref> <ref>PMID:12475940</ref> <ref>PMID:14627716</ref> <ref>PMID:21454883</ref>

-	~~Authors~~: ~~Zanetti, G~~.~~, Prinz, S~~.~~, Daum, S~~.~~, Meister, A., Schekman, R., Bacia, K~~.~~, Briggs, J~~.A.G.	+	==About this Structure==
		+	[[4bzk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZK OCA].

-	~~Description~~: ~~The structure of the COPII coat assembled on membranes~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Bacia, K.]]
		+	[[Category: Briggs, J A.G.]]
		+	[[Category: Daum, S.]]
		+	[[Category: Meister, A.]]
		+	[[Category: Prinz, S.]]
		+	[[Category: Schekman, R.]]
		+	[[Category: Zanetti, G.]]
		+	[[Category: Protein transport]]
		+	[[Category: Secretion]]
		+	[[Category: Trafficking]]