4c0a - Revision history

OCA at 12:01, 20 December 2023

OCA — Wed, 20 Dec 2023 12:01:17 GMT

←Older revision		Revision as of 12:01, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [https://pdbe.org/4c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [https://www.ebi.ac.uk/pdbsum/4c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [https://pdbe.org/4c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [https://www.ebi.ac.uk/pdbsum/4c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IQEC1_HUMAN IQEC1_HUMAN]] In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.<ref>PMID:11226253</ref> <ref>PMID:16461286</ref>	+	[https://www.uniprot.org/uniprot/IQEC1_HUMAN IQEC1_HUMAN] In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.<ref>PMID:11226253</ref> <ref>PMID:16461286</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mechanisms whereby guanine nucleotide exchange factors (GEFs) coordinate their subcellular targeting to their activation of small GTPases remain poorly understood. Here we analyzed how membranes control the efficiency of human BRAG2, an ArfGEF involved in receptor endocytosis, Wnt signaling, and tumor invasion. The crystal structure of an Arf1-BRAG2 complex that mimics a membrane-bound intermediate revealed an atypical PH domain that is constitutively anchored to the catalytic Sec7 domain and interacts with Arf. Combined with the quantitative analysis of BRAG2 exchange activity reconstituted on membranes, we find that this PH domain potentiates nucleotide exchange by about 2,000-fold by cumulative conformational and membrane-targeting contributions. Furthermore, it restricts BRAG2 activity to negatively charged membranes without phosphoinositide specificity, using a positively charged surface peripheral to but excluding the canonical lipid-binding pocket. This suggests a model of BRAG2 regulation along the early endosomal pathway that expands the repertoire of GEF regulatory mechanisms. Notably, it departs from the auto-inhibitory and feedback loop paradigm emerging from studies of SOS and cytohesins. It also uncovers a novel mechanism of unspecific lipid-sensing by PH domains that may allow sustained binding to maturating membranes.
		+
		+	Integrated Conformational and Lipid-Sensing Regulation of Endosomal ArfGEF BRAG2.,Aizel K, Biou V, Navaza J, Duarte LV, Campanacci V, Cherfils J, Zeghouf M PLoS Biol. 2013 Sep;11(9):e1001652. doi: 10.1371/journal.pbio.1001652. Epub 2013 , Sep 10. PMID:24058294<ref>PMID:24058294</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4c0a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:19, 7 September 2022

OCA — Wed, 07 Sep 2022 17:19:01 GMT

←Older revision		Revision as of 17:19, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4c0a' size='340' side='right'caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4c0a' size='340' side='right'caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4C0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [~~http~~://pdbe.org/4c0a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4c0a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [https://pdbe.org/4c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [https://www.ebi.ac.uk/pdbsum/4c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IQEC1_HUMAN IQEC1_HUMAN]] In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.<ref>PMID:11226253</ref> <ref>PMID:16461286</ref> [[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.	+	[[https://www.uniprot.org/uniprot/IQEC1_HUMAN IQEC1_HUMAN]] In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.<ref>PMID:11226253</ref> <ref>PMID:16461286</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The mechanisms whereby guanine nucleotide exchange factors (GEFs) coordinate their subcellular targeting to their activation of small GTPases remain poorly understood. Here we analyzed how membranes control the efficiency of human BRAG2, an ArfGEF involved in receptor endocytosis, Wnt signaling, and tumor invasion. The crystal structure of an Arf1-BRAG2 complex that mimics a membrane-bound intermediate revealed an atypical PH domain that is constitutively anchored to the catalytic Sec7 domain and interacts with Arf. Combined with the quantitative analysis of BRAG2 exchange activity reconstituted on membranes, we find that this PH domain potentiates nucleotide exchange by about 2,000-fold by cumulative conformational and membrane-targeting contributions. Furthermore, it restricts BRAG2 activity to negatively charged membranes without phosphoinositide specificity, using a positively charged surface peripheral to but excluding the canonical lipid-binding pocket. This suggests a model of BRAG2 regulation along the early endosomal pathway that expands the repertoire of GEF regulatory mechanisms. Notably, it departs from the auto-inhibitory and feedback loop paradigm emerging from studies of SOS and cytohesins. It also uncovers a novel mechanism of unspecific lipid-sensing by PH domains that may allow sustained binding to maturating membranes.	+
-		+
-	Integrated Conformational and Lipid-Sensing Regulation of Endosomal ArfGEF BRAG2.,Aizel K, Biou V, Navaza J, Duarte LV, Campanacci V, Cherfils J, Zeghouf M PLoS Biol. 2013 Sep;11(9):e1001652. doi: 10.1371/journal.pbio.1001652. Epub 2013 , Sep 10. PMID:24058294<ref>PMID:24058294</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4c0a" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aizel, K]]	+	[[Category: Aizel K]]
-	[[Category: Biou, V]]	+	[[Category: Biou V]]
-	[[Category: Campanacci, V]]	+	[[Category: Campanacci V]]
-	[[Category: Cherfils, J]]	+	[[Category: Cherfils J]]
-	[[Category: Duarte, L]]	+	[[Category: Duarte L]]
-	[[Category: Navaza, J]]	+	[[Category: Navaza J]]
-	[[Category: Zeghouf, M]]	+	[[Category: Zeghouf M]]
-	~~[[Category: Endocytosis]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 13:42, 13 March 2019

OCA — Wed, 13 Mar 2019 13:42:25 GMT

←Older revision		Revision as of 13:42, 13 March 2019
Line 1:		Line 1:

	==Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain==		==Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain==
-	<StructureSection load='4c0a' size='340' side='right' caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='4c0a' size='340' side='right'caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0A FirstGlance]. <br>
Line 24:		Line 24:
	[[Category: Bovin]]		[[Category: Bovin]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aizel, K]]		[[Category: Aizel, K]]
	[[Category: Biou, V]]		[[Category: Biou, V]]

OCA at 21:52, 5 August 2016

OCA — Fri, 05 Aug 2016 21:52:53 GMT

←Older revision		Revision as of 21:52, 5 August 2016
Line 1:		Line 1:
		+
	==Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain==		==Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain==
	<StructureSection load='4c0a' size='340' side='right' caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4c0a' size='340' side='right' caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [http://www.ebi.ac.uk/pdbsum/4c0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [http://pdbe.org/4c0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [http://www.ebi.ac.uk/pdbsum/4c0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.	+	[[http://www.uniprot.org/uniprot/IQEC1_HUMAN IQEC1_HUMAN]] In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.<ref>PMID:11226253</ref> <ref>PMID:16461286</ref> [[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4c0a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Aizel, K]]		[[Category: Aizel, K]]
	[[Category: Biou, V]]		[[Category: Biou, V]]

OCA at 22:13, 25 December 2014

OCA — Thu, 25 Dec 2014 22:13:23 GMT

←Older revision		Revision as of 22:13, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [http://www.ebi.ac.uk/pdbsum/4c0a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [http://www.ebi.ac.uk/pdbsum/4c0a PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:52, 21 December 2014

OCA — Sun, 21 Dec 2014 09:52:02 GMT

←Older revision		Revision as of 09:52, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4c0a\| PDB=4c0a \| SCENE= }}~~	+	==Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain==
-	===Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain===	+	<StructureSection load='4c0a' size='340' side='right' caption='[[4c0a]], [[Resolution\|resolution]] 3.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24058294}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c0a RCSB], [http://www.ebi.ac.uk/pdbsum/4c0a PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mechanisms whereby guanine nucleotide exchange factors (GEFs) coordinate their subcellular targeting to their activation of small GTPases remain poorly understood. Here we analyzed how membranes control the efficiency of human BRAG2, an ArfGEF involved in receptor endocytosis, Wnt signaling, and tumor invasion. The crystal structure of an Arf1-BRAG2 complex that mimics a membrane-bound intermediate revealed an atypical PH domain that is constitutively anchored to the catalytic Sec7 domain and interacts with Arf. Combined with the quantitative analysis of BRAG2 exchange activity reconstituted on membranes, we find that this PH domain potentiates nucleotide exchange by about 2,000-fold by cumulative conformational and membrane-targeting contributions. Furthermore, it restricts BRAG2 activity to negatively charged membranes without phosphoinositide specificity, using a positively charged surface peripheral to but excluding the canonical lipid-binding pocket. This suggests a model of BRAG2 regulation along the early endosomal pathway that expands the repertoire of GEF regulatory mechanisms. Notably, it departs from the auto-inhibitory and feedback loop paradigm emerging from studies of SOS and cytohesins. It also uncovers a novel mechanism of unspecific lipid-sensing by PH domains that may allow sustained binding to maturating membranes.

-	~~==Function==~~	+	Integrated Conformational and Lipid-Sensing Regulation of Endosomal ArfGEF BRAG2.,Aizel K, Biou V, Navaza J, Duarte LV, Campanacci V, Cherfils J, Zeghouf M PLoS Biol. 2013 Sep;11(9):e1001652. doi: 10.1371/journal.pbio.1001652. Epub 2013 , Sep 10. PMID:24058294<ref>PMID:24058294</ref>
-	~~[[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP~~-~~binding protein that functions as an allosteric activator~~ of ~~the cholera toxin catalytic subunit~~, ~~an ADP-ribosyltransferase~~. ~~Involved in protein trafficking among different compartments~~. ~~Modulates vesicle budding and uncoating within the Golgi complex~~. ~~Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking~~. ~~In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane~~. ~~The hydrolysis of ARF1-bound GTP~~, ~~which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4c0a]] is~~ a ~~8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus] and [http://en~~.~~wikipedia.org~~/~~wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024058294</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Aizel, K.]]	+	[[Category: Aizel, K]]
-	[[Category: Biou, V.]]	+	[[Category: Biou, V]]
-	[[Category: Campanacci, V.]]	+	[[Category: Campanacci, V]]
-	[[Category: Cherfils, J.]]	+	[[Category: Cherfils, J]]
-	[[Category: Duarte, L.]]	+	[[Category: Duarte, L]]
-	[[Category: Navaza, J.]]	+	[[Category: Navaza, J]]
-	[[Category: Zeghouf, M.]]	+	[[Category: Zeghouf, M]]
	[[Category: Endocytosis]]		[[Category: Endocytosis]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 06:22, 2 October 2013

OCA — Wed, 02 Oct 2013 06:22:10 GMT

←Older revision		Revision as of 06:22, 2 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4c0a\| PDB=4c0a \| SCENE= }}		{{STRUCTURE_4c0a\| PDB=4c0a \| SCENE= }}
	===Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain===		===Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain===
		+	{{ABSTRACT_PUBMED_24058294}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA].		[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024058294</ref><references group="xtra"/><references/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 08:16, 29 September 2013

OCA — Sun, 29 Sep 2013 08:16:40 GMT

←Older revision		Revision as of 08:16, 29 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4c0a\| PDB=4c0a \| SCENE= }}
		+	===Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain===

-	The ~~entry 4c0a~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ARF1_BOVIN ARF1_BOVIN]] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

-	~~Authors~~: Aizel, K., Biou, V., ~~Navaza~~, J., Duarte, L.~~, Campanacci, V., Cherfils~~, J., Zeghouf, M.	+	==About this Structure==
-		+	[[4c0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0A OCA].
-	~~Description~~: ~~Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain~~	+	[[Category: Bos taurus]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Aizel, K.]]
		+	[[Category: Biou, V.]]
		+	[[Category: Campanacci, V.]]
		+	[[Category: Cherfils, J.]]
		+	[[Category: Duarte, L.]]
		+	[[Category: Navaza, J.]]
		+	[[Category: Zeghouf, M.]]
		+	[[Category: Endocytosis]]
		+	[[Category: Protein transport]]

OCA at 03:17, 20 September 2013

OCA — Fri, 20 Sep 2013 03:17:03 GMT

←Older revision		Revision as of 03:17, 20 September 2013
Line 3:		Line 3:
	The entry 4c0a is ON HOLD		The entry 4c0a is ON HOLD

-	Authors: Aizel, K., Biou, V., Navaza, J., ~~Zeghouf~~, M., Cherfils, J.	+	Authors: Aizel, K., Biou, V., Navaza, J., Duarte, L., Campanacci, V., Cherfils, J., Zeghouf, M.

	Description: Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain		Description: Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain

OCA at 06:22, 28 August 2013

OCA — Wed, 28 Aug 2013 06:22:03 GMT

←Older revision		Revision as of 06:22, 28 August 2013
Line 3:		Line 3:
	The entry 4c0a is ON HOLD		The entry 4c0a is ON HOLD

-	Authors: Aizel, K., Biou, V., Navaza, J., ~~Duarte~~, ~~L., Campanacci, V~~., Cherfils, J~~., Zeghouf, M~~.	+	Authors: Aizel, K., Biou, V., Navaza, J., Zeghouf, M., Cherfils, J.

	Description: Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain		Description: Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain