4c3s - Revision history

OCA at 12:02, 20 December 2023

OCA — Wed, 20 Dec 2023 12:02:57 GMT

←Older revision		Revision as of 12:02, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnoclostridium_phytofermentans Lachnoclostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C3S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnoclostridium_phytofermentans Lachnoclostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [https://pdbe.org/4c3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [https://www.ebi.ac.uk/pdbsum/4c3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [https://pdbe.org/4c3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [https://www.ebi.ac.uk/pdbsum/4c3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A9KN57_LACP7 A9KN57_LACP7]]	+	[https://www.uniprot.org/uniprot/A9KN57_LACP7 A9KN57_LACP7]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The breakdown of fucose and rhamnose released from plant cell walls by the cellulolytic soil bacterium Clostridium phytofermentans produces toxic aldehyde intermediates. To enable growth on these carbon sources, the pathway for the breakdown of fucose and rhamnose is encapsulated within a bacterial microcompartment (BMC). These proteinaceous organelles sequester the toxic aldehyde intermediates and allow the efficient action of acylating aldehyde dehydrogenase enzymes to produce an acyl-CoA that is ultimately used in substrate-level phosphorylation to produce ATP. Here we analyse the kinetics of the aldehyde dehydrogenase enzyme from the fucose/rhamnose utilisation BMC with different short-chain fatty aldehydes and show that it has activity against substrates with up to six carbon atoms, with optimal activity against propionaldehyde. We have also determined the X-ray crystal structure of this enzyme in complex with CoA and show that the adenine nucleotide of this cofactor is bound in a distinct pocket to the same group in NAD(+). This work is the first report of the structure of CoA bound to an aldehyde dehydrogenase enzyme and our crystallographic model provides important insight into the differences within the active site that distinguish the acylating from non-acylating aldehyde dehydrogenase enzymes.
		+
		+	Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.,Tuck LR, Altenbach K, Ang TF, Crawshaw AD, Campopiano DJ, Clarke DJ, Marles-Wright J Sci Rep. 2016 Feb 22;6:22108. doi: 10.1038/srep22108. PMID:26899032<ref>PMID:26899032</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:24, 7 September 2022

OCA — Wed, 07 Sep 2022 17:24:40 GMT

←Older revision		Revision as of 17:24, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4c3s' size='340' side='right'caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='4c3s' size='340' side='right'caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"lachnoclostridium_phytofermentans"_yutin_and_galperin_2013 "lachnoclostridium~~ phytofermentans~~" yutin and galperin 2013~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4C3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnoclostridium_phytofermentans Lachnoclostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [https://pdbe.org/4c3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [https://www.ebi.ac.uk/pdbsum/4c3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3s ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [~~http~~://pdbe.org/4c3s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4c3s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3s ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/A9KN57_LACP7 A9KN57_LACP7]]
-	The breakdown of fucose and rhamnose released from plant cell walls by the cellulolytic soil bacterium Clostridium phytofermentans produces toxic aldehyde intermediates. To enable growth on these carbon sources, the pathway for the breakdown of fucose and rhamnose is encapsulated within a bacterial microcompartment (BMC). These proteinaceous organelles sequester the toxic aldehyde intermediates and allow the efficient action of acylating aldehyde dehydrogenase enzymes to produce an acyl-CoA that is ultimately used in substrate-level phosphorylation to produce ATP. Here we analyse the kinetics of the aldehyde dehydrogenase enzyme from the fucose/rhamnose utilisation BMC with different short-chain fatty aldehydes and show that it has activity against substrates with up to six carbon atoms, with optimal activity against propionaldehyde. We have also determined the X-ray crystal structure of this enzyme in complex with CoA and show that the adenine nucleotide of this cofactor is bound in a distinct pocket to the same group in NAD(+). This work is the first report of the structure of CoA bound to an aldehyde dehydrogenase enzyme and our crystallographic model provides important insight into the differences within the active site that distinguish the acylating from non-acylating aldehyde dehydrogenase enzymes.	+
-		+
-	Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.,Tuck LR, Altenbach K, Ang TF, Crawshaw AD, Campopiano DJ, Clarke DJ, Marles-Wright J Sci Rep. 2016 Feb 22;6:~~22108. doi: 10.1038~~/~~srep22108. PMID:26899032<ref>PMID:26899032<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Lachnoclostridium phytofermentans ~~yutin and galperin 2013~~]]	+	[[Category: Lachnoclostridium phytofermentans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Altenbach, K]]	+	[[Category: Altenbach K]]
-	[[Category: Ang~~, T F~~]]	+	[[Category: Ang TF]]
-	[[Category: Crawshaw, A]]	+	[[Category: Crawshaw A]]
-	[[Category: Marles-Wright, J]]	+	[[Category: Marles-Wright J]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 12:46, 10 May 2019

OCA — Fri, 10 May 2019 12:46:29 GMT

←Older revision		Revision as of 12:46, 10 May 2019
Line 1:		Line 1:

	==Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment==		==Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment==
-	<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>	+	<StructureSection load='4c3s' size='340' side='right'caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lachnoclostridium_phytofermentans"_yutin_and_galperin_2013 "lachnoclostridium phytofermentans" yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lachnoclostridium_phytofermentans"_yutin_and_galperin_2013 "lachnoclostridium phytofermentans" yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Lachnoclostridium phytofermentans yutin and galperin 2013]]		[[Category: Lachnoclostridium phytofermentans yutin and galperin 2013]]
		+	[[Category: Large Structures]]
	[[Category: Altenbach, K]]		[[Category: Altenbach, K]]
	[[Category: Ang, T F]]		[[Category: Ang, T F]]

OCA at 12:55, 16 November 2017

OCA — Thu, 16 Nov 2017 12:55:29 GMT

←Older revision		Revision as of 12:55, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_700394 Atcc 700394~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lachnoclostridium_phytofermentans"_yutin_and_galperin_2013 "lachnoclostridium phytofermentans" yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [http://pdbe.org/4c3s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [http://www.ebi.ac.uk/pdbsum/4c3s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [http://pdbe.org/4c3s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [http://www.ebi.ac.uk/pdbsum/4c3s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3s ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 700394~~]]	+	[[Category: Lachnoclostridium phytofermentans yutin and galperin 2013]]
	[[Category: Altenbach, K]]		[[Category: Altenbach, K]]
	[[Category: Ang, T F]]		[[Category: Ang, T F]]

OCA at 16:31, 10 May 2016

OCA — Tue, 10 May 2016 16:31:38 GMT

←Older revision		Revision as of 16:31, 10 May 2016
Line 1:		Line 1:
		+
	==Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment==		==Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment==
	<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_phytofermentans Clostridium phytofermentans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700394 Atcc 700394]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [http://www.ebi.ac.uk/pdbsum/4c3s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [http://pdbe.org/4c3s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [http://www.ebi.ac.uk/pdbsum/4c3s PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The breakdown of fucose and rhamnose released from plant cell walls by the cellulolytic soil bacterium Clostridium phytofermentans produces toxic aldehyde intermediates. To enable growth on these carbon sources, the pathway for the breakdown of fucose and rhamnose is encapsulated within a bacterial microcompartment (BMC). These proteinaceous organelles sequester the toxic aldehyde intermediates and allow the efficient action of acylating aldehyde dehydrogenase enzymes to produce an acyl-CoA that is ultimately used in substrate-level phosphorylation to produce ATP. Here we analyse the kinetics of the aldehyde dehydrogenase enzyme from the fucose/rhamnose utilisation BMC with different short-chain fatty aldehydes and show that it has activity against substrates with up to six carbon atoms, with optimal activity against propionaldehyde. We have also determined the X-ray crystal structure of this enzyme in complex with CoA and show that the adenine nucleotide of this cofactor is bound in a distinct pocket to the same group in NAD(+). This work is the first report of the structure of CoA bound to an aldehyde dehydrogenase enzyme and our crystallographic model provides important insight into the differences within the active site that distinguish the acylating from non-acylating aldehyde dehydrogenase enzymes.
		+
		+	Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.,Tuck LR, Altenbach K, Ang TF, Crawshaw AD, Campopiano DJ, Clarke DJ, Marles-Wright J Sci Rep. 2016 Feb 22;6:22108. doi: 10.1038/srep22108. PMID:26899032<ref>PMID:26899032</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4c3s" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium phytofermentans~~]]	+	[[Category: Atcc 700394]]
	[[Category: Altenbach, K]]		[[Category: Altenbach, K]]
	[[Category: Ang, T F]]		[[Category: Ang, T F]]

OCA at 10:17, 20 January 2015

OCA — Tue, 20 Jan 2015 10:17:27 GMT

←Older revision		Revision as of 10:17, 20 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4c3s\| PDB=4c3s \| SCENE= }}~~	+	==Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment==
-	===Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment===	+	<StructureSection load='4c3s' size='340' side='right' caption='[[4c3s]], [[Resolution\|resolution]] 1.64Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_phytofermentans Clostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c3s RCSB], [http://www.ebi.ac.uk/pdbsum/4c3s PDBsum]</span></td></tr>
		+	</table>

-	==~~About this Structure~~==	+	==See Also==
-	[[~~4c3s~~]] ~~is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Clostridium_phytofermentans Clostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA].~~	+	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium phytofermentans]]		[[Category: Clostridium phytofermentans]]
-	[[Category: Altenbach, K.]]	+	[[Category: Altenbach, K]]
-	[[Category: Ang, T F.]]	+	[[Category: Ang, T F]]
-	[[Category: Crawshaw, A.]]	+	[[Category: Crawshaw, A]]
-	[[Category: Marles-Wright, J.]]	+	[[Category: Marles-Wright, J]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 05:29, 12 September 2013

OCA — Thu, 12 Sep 2013 05:29:30 GMT

←Older revision		Revision as of 05:29, 12 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4c3s\| PDB=4c3s \| SCENE= }}
		+	===Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment===

-	~~The entry~~ 4c3s is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4c3s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_phytofermentans Clostridium phytofermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3S OCA].
-	~~Authors~~: ~~Marles-Wright~~, J.~~, Crawshaw, A.,~~ Ang, T.F., ~~Altenbach~~, K.	+	[[Category: Clostridium phytofermentans]]
-		+	[[Category: Altenbach, K.]]
-	~~Description~~: ~~Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment~~	+	[[Category: Ang, T F.]]
		+	[[Category: Crawshaw, A.]]
		+	[[Category: Marles-Wright, J.]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "4c3s" [edit=sysop:move=sysop]

OCA — Wed, 04 Sep 2013 09:31:59 GMT

Protected "4c3s" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:31, 4 September 2013

OCA: New page: '''Unreleased structure''' The entry 4c3s is ON HOLD Authors: Marles-Wright, J., Crawshaw, A., Ang, T.F., Altenbach, K. Description: Structure of a propionaldehyde dehydrogenase from t...

OCA — Wed, 04 Sep 2013 09:31:57 GMT

New page: '''Unreleased structure''' The entry 4c3s is ON HOLD Authors: Marles-Wright, J., Crawshaw, A., Ang, T.F., Altenbach, K. Description: Structure of a propionaldehyde dehydrogenase from t...

New page

'''Unreleased structure'''

The entry 4c3s is ON HOLD

Authors: Marles-Wright, J., Crawshaw, A., Ang, T.F., Altenbach, K.

Description: Structure of a propionaldehyde dehydrogenase from the Clostridium phytofermentans fucose utilisation bacterial microcompartment