4c9e - Revision history

OCA at 17:33, 7 September 2022

OCA — Wed, 07 Sep 2022 17:33:12 GMT

←Older revision		Revision as of 17:33, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4c9e' size='340' side='right'caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4c9e' size='340' side='right'caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c9e]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~] and [~~http~~://en.wikipedia.org/wiki/~~Silurana_(xenopus)_tropicalis Silurana (xenopus)~~ tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4C9E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c9e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Xenopus_tropicalis Xenopus tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C9E FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c84\|4c84]], [[4c85\|4c85]], [[4c86\|4c86]], [[4c8a\|4c8a]], [[4c8c\|4c8c]], [[4c8f\|4c8f]], [[4c8p\|4c8p]], [[4c8t\|4c8t]], [[4c8u\|4c8u]], [[4c8v\|4c8v]], [[4c8w\|4c8w]], [[4c99\|4c99]], [[4c9a\|4c9a]], [[4c9r\|4c9r]], [[4c9u\|4c9u]], [[4c9v\|4c9v]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c9e OCA], [https://pdbe.org/4c9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c9e RCSB], [https://www.ebi.ac.uk/pdbsum/4c9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c9e ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4c9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c9e OCA], [~~http~~://pdbe.org/4c9e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4c9e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4c9e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4c9e ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ZNRF3_MOUSE ZNRF3_MOUSE]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:22575959</ref> <ref>PMID:22895187</ref> [[http://www.uniprot.org/uniprot/RSPO2_XENTR RSPO2_XENTR]] Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Activates neural markers and promotes muscle formation. Overexpression blocks activin, nodal and BMP4 signaling, suggesting that it may negatively regulate the TGF-beta pathway (By similarity).	+	[[https://www.uniprot.org/uniprot/ZNRF3_MOUSE ZNRF3_MOUSE]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:22575959</ref> <ref>PMID:22895187</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The four R-spondin (Rspo) proteins are secreted agonists of Wnt signalling in vertebrates, functioning in embryogenesis and adult stem cell biology. Through ubiquitination and degradation of Wnt receptors, the transmembrane E3 ubiquitin ligase ZNRF3 and related RNF43 antagonize Wnt signalling. Rspo ligands have been reported to inhibit the ligase activity through direct interaction with ZNRF3 and RNF43. Here we report multiple crystal structures of the ZNRF3 ectodomain (ZNRF3ecto), a signalling-competent Furin1-Furin2 (Fu1-Fu2) fragment of Rspo2 (Rspo2Fu1-Fu2), and Rspo2Fu1-Fu2 in complex with ZNRF3ecto, or RNF43ecto. A prominent loop in Fu1 clamps into equivalent grooves in the ZNRF3ecto and RNF43ecto surface. Rspo binding enhances dimerization of ZNRF3ecto but not of RNF43ecto. Comparison of the four Rspo proteins, mutants and chimeras in biophysical and cellular assays shows that their signalling potency depends on their ability to recruit ZNRF3 or RNF43 via Fu1 into a complex with LGR receptors, which interact with Rspo via Fu2.	+
-		+
-	Structural and molecular basis of ZNRF3/RNF43 transmembrane ubiquitin ligase inhibition by the Wnt agonist R-spondin.,Zebisch M, Xu Y, Krastev C, Macdonald BT, Chen M, Gilbert RJ, He X, Jones EY Nat Commun. 2013 Nov 14;4:2787. doi: 10.1038/ncomms3787. PMID:24225776<ref>PMID:24225776</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4c9e" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
-	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]	+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Jones, E Y~~]]	+	[[Category: Xenopus tropicalis]]
-	[[Category: ~~Zebisch, M~~]]	+	[[Category: Jones EY]]
-	[[Category: ~~Lgr4]]~~	+	[[Category: Zebisch M]]
-	~~[[Category: Lgr5]]~~	+
-	~~[[Category: Lgr6]]~~	+
-	~~[[Category: Ligase-signaling protein complex]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: R-spo]]~~	+
-	~~[[Category: Receptor]]~~	+
-	~~[[Category: Rnf43]]~~	+
-	~~[[Category: Rspo]]~~	+
-	~~[[Category: Rspo1]]~~	+
-	~~[[Category: Rspo3]]~~	+
-	~~[[Category: Rspo4]]~~	+
-	~~[[Category: Signalling]]~~	+
-	~~[[Category: Wnt~~]]	+

OCA at 09:53, 20 March 2019

OCA — Wed, 20 Mar 2019 09:53:06 GMT

←Older revision		Revision as of 09:53, 20 March 2019
Line 1:		Line 1:

	==Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II==		==Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II==
-	<StructureSection load='4c9e' size='340' side='right' caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4c9e' size='340' side='right'caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c9e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Silurana_(xenopus)_tropicalis Silurana (xenopus) tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C9E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c9e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Silurana_(xenopus)_tropicalis Silurana (xenopus) tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C9E FirstGlance]. <br>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Jones, E Y]]		[[Category: Jones, E Y]]

OCA at 15:28, 5 August 2016

OCA — Fri, 05 Aug 2016 15:28:46 GMT

←Older revision		Revision as of 15:28, 5 August 2016
Line 1:		Line 1:
		+
	==Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II==		==Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II==
	<StructureSection load='4c9e' size='340' side='right' caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4c9e' size='340' side='right' caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c84\|4c84]], [[4c85\|4c85]], [[4c86\|4c86]], [[4c8a\|4c8a]], [[4c8c\|4c8c]], [[4c8f\|4c8f]], [[4c8p\|4c8p]], [[4c8t\|4c8t]], [[4c8u\|4c8u]], [[4c8v\|4c8v]], [[4c8w\|4c8w]], [[4c99\|4c99]], [[4c9a\|4c9a]], [[4c9r\|4c9r]], [[4c9u\|4c9u]], [[4c9v\|4c9v]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c84\|4c84]], [[4c85\|4c85]], [[4c86\|4c86]], [[4c8a\|4c8a]], [[4c8c\|4c8c]], [[4c8f\|4c8f]], [[4c8p\|4c8p]], [[4c8t\|4c8t]], [[4c8u\|4c8u]], [[4c8v\|4c8v]], [[4c8w\|4c8w]], [[4c99\|4c99]], [[4c9a\|4c9a]], [[4c9r\|4c9r]], [[4c9u\|4c9u]], [[4c9v\|4c9v]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c9e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c9e RCSB], [http://www.ebi.ac.uk/pdbsum/4c9e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c9e OCA], [http://pdbe.org/4c9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c9e RCSB], [http://www.ebi.ac.uk/pdbsum/4c9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c9e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4c9e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 03:36, 25 December 2014

OCA — Thu, 25 Dec 2014 03:36:30 GMT

←Older revision		Revision as of 03:36, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4c9e\| PDB=4c9e \| SCENE= }}~~	+	==Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II==
-	===Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II===	+	<StructureSection load='4c9e' size='340' side='right' caption='[[4c9e]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24225776}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4c9e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Silurana_(xenopus)_tropicalis Silurana (xenopus) tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C9E FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c84\|4c84]], [[4c85\|4c85]], [[4c86\|4c86]], [[4c8a\|4c8a]], [[4c8c\|4c8c]], [[4c8f\|4c8f]], [[4c8p\|4c8p]], [[4c8t\|4c8t]], [[4c8u\|4c8u]], [[4c8v\|4c8v]], [[4c8w\|4c8w]], [[4c99\|4c99]], [[4c9a\|4c9a]], [[4c9r\|4c9r]], [[4c9u\|4c9u]], [[4c9v\|4c9v]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c9e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c9e RCSB], [http://www.ebi.ac.uk/pdbsum/4c9e PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/ZNRF3_MOUSE ZNRF3_MOUSE]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:22575959</ref> <ref>PMID:22895187</ref> [[http://www.uniprot.org/uniprot/RSPO2_XENTR RSPO2_XENTR]] Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Activates neural markers and promotes muscle formation. Overexpression blocks activin, nodal and BMP4 signaling, suggesting that it may negatively regulate the TGF-beta pathway (By similarity).		[[http://www.uniprot.org/uniprot/ZNRF3_MOUSE ZNRF3_MOUSE]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:22575959</ref> <ref>PMID:22895187</ref> [[http://www.uniprot.org/uniprot/RSPO2_XENTR RSPO2_XENTR]] Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Activates neural markers and promotes muscle formation. Overexpression blocks activin, nodal and BMP4 signaling, suggesting that it may negatively regulate the TGF-beta pathway (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The four R-spondin (Rspo) proteins are secreted agonists of Wnt signalling in vertebrates, functioning in embryogenesis and adult stem cell biology. Through ubiquitination and degradation of Wnt receptors, the transmembrane E3 ubiquitin ligase ZNRF3 and related RNF43 antagonize Wnt signalling. Rspo ligands have been reported to inhibit the ligase activity through direct interaction with ZNRF3 and RNF43. Here we report multiple crystal structures of the ZNRF3 ectodomain (ZNRF3ecto), a signalling-competent Furin1-Furin2 (Fu1-Fu2) fragment of Rspo2 (Rspo2Fu1-Fu2), and Rspo2Fu1-Fu2 in complex with ZNRF3ecto, or RNF43ecto. A prominent loop in Fu1 clamps into equivalent grooves in the ZNRF3ecto and RNF43ecto surface. Rspo binding enhances dimerization of ZNRF3ecto but not of RNF43ecto. Comparison of the four Rspo proteins, mutants and chimeras in biophysical and cellular assays shows that their signalling potency depends on their ability to recruit ZNRF3 or RNF43 via Fu1 into a complex with LGR receptors, which interact with Rspo via Fu2.
		+
		+	Structural and molecular basis of ZNRF3/RNF43 transmembrane ubiquitin ligase inhibition by the Wnt agonist R-spondin.,Zebisch M, Xu Y, Krastev C, Macdonald BT, Chen M, Gilbert RJ, He X, Jones EY Nat Commun. 2013 Nov 14;4:2787. doi: 10.1038/ncomms3787. PMID:24225776<ref>PMID:24225776</ref>

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4c9e]] is~~ a ~~8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en~~.~~wikipedia.org~~/~~wiki/Silurana_(xenopus)_tropicalis Silurana (xenopus) tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:024225776</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
-	[[Category: Jones, E Y.]]	+	[[Category: Jones, E Y]]
-	[[Category: Zebisch, M.]]	+	[[Category: Zebisch, M]]
	[[Category: Lgr4]]		[[Category: Lgr4]]
	[[Category: Lgr5]]		[[Category: Lgr5]]

OCA at 08:49, 27 November 2013

OCA — Wed, 27 Nov 2013 08:49:19 GMT

←Older revision		Revision as of 08:49, 27 November 2013
Line 1:		Line 1:
	{{STRUCTURE_4c9e\| PDB=4c9e \| SCENE= }}		{{STRUCTURE_4c9e\| PDB=4c9e \| SCENE= }}
	===Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II===		===Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II===
		+	{{ABSTRACT_PUBMED_24225776}}

	==Function==		==Function==
Line 6:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4c9e]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA].	+	[[4c9e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Silurana_(xenopus)_tropicalis Silurana (xenopus) tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA].

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024225776</ref><references group="xtra"/><references/>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Jones, E Y.]]		[[Category: Jones, E Y.]]
	[[Category: Zebisch, M.]]		[[Category: Zebisch, M.]]

OCA at 13:40, 20 November 2013

OCA — Wed, 20 Nov 2013 13:40:31 GMT

←Older revision		Revision as of 13:40, 20 November 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4c9e\| PDB=4c9e \| SCENE= }}
		+	===Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II===

-	~~The entry 4c9e is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ZNRF3_MOUSE ZNRF3_MOUSE]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:22575959</ref> <ref>PMID:22895187</ref> [[http://www.uniprot.org/uniprot/RSPO2_XENTR RSPO2_XENTR]] Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Activates neural markers and promotes muscle formation. Overexpression blocks activin, nodal and BMP4 signaling, suggesting that it may negatively regulate the TGF-beta pathway (By similarity).

-	~~Authors~~: ~~Zebisch, M~~.~~, Jones, E~~.Y.	+	==About this Structure==
		+	[[4c9e]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C9E OCA].

-	~~Description~~: ~~Mouse ZNRF3 ectodomain in~~ complex ~~with Xenopus RSPO2 Fu1~~-~~Fu2 (Seleno Met) crystal form II~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Jones, E Y.]]
		+	[[Category: Zebisch, M.]]
		+	[[Category: Lgr4]]
		+	[[Category: Lgr5]]
		+	[[Category: Lgr6]]
		+	[[Category: Ligase-signaling protein complex]]
		+	[[Category: Membrane]]
		+	[[Category: R-spo]]
		+	[[Category: Receptor]]
		+	[[Category: Rnf43]]
		+	[[Category: Rspo]]
		+	[[Category: Rspo1]]
		+	[[Category: Rspo3]]
		+	[[Category: Rspo4]]
		+	[[Category: Signalling]]
		+	[[Category: Wnt]]

OCA: Protected "4c9e" [edit=sysop:move=sysop]

OCA — Thu, 10 Oct 2013 05:29:09 GMT

Protected "4c9e" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:29, 10 October 2013

OCA: New page: '''Unreleased structure''' The entry 4c9e is ON HOLD Authors: Zebisch, M., Jones, E.Y. Description: Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal fo...

OCA — Thu, 10 Oct 2013 05:29:08 GMT

New page: '''Unreleased structure''' The entry 4c9e is ON HOLD Authors: Zebisch, M., Jones, E.Y. Description: Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal fo...

New page

'''Unreleased structure'''

The entry 4c9e is ON HOLD

Authors: Zebisch, M., Jones, E.Y.

Description: Mouse ZNRF3 ectodomain in complex with Xenopus RSPO2 Fu1-Fu2 (Seleno Met) crystal form II