4cag - Revision history

OCA at 12:06, 20 December 2023

OCA — Wed, 20 Dec 2023 12:06:39 GMT

←Older revision		Revision as of 12:06, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CAG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CAG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.498Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [https://pdbe.org/4cag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [https://www.ebi.ac.uk/pdbsum/4cag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [https://pdbe.org/4cag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [https://www.ebi.ac.uk/pdbsum/4cag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q65KY4_BACLD Q65KY4_BACLD]]	+	[https://www.uniprot.org/uniprot/Q65KY4_BACLD Q65KY4_BACLD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.-) catalyze cleavage of alpha-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by beta-elimination. In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions. These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT). The second-generation mutants involved combinations of two to seven individually favorable single mutations. Thermal stability was examined as half-life at 60 degrees C and by recording of thermal transitions by circular dichroism. Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme. A ~ twofold improvement in thermal stability at 60 degrees C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type. The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
		+
		+	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.,Silva IR, Jers C, Otten H, Nyffenegger C, Larsen DM, Derkx PM, Meyer AS, Mikkelsen JD, Larsen S Appl Microbiol Biotechnol. 2014 May;98(10):4521-31. doi:, 10.1007/s00253-013-5483-8. Epub 2014 Jan 14. PMID:24419797<ref>PMID:24419797</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cag" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:34, 7 September 2022

OCA — Wed, 07 Sep 2022 17:34:44 GMT

←Older revision		Revision as of 17:34, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4cag' size='340' side='right'caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4cag' size='340' side='right'caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CAG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CAG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [~~http~~://pdbe.org/4cag PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cag PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [https://pdbe.org/4cag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [https://www.ebi.ac.uk/pdbsum/4cag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/Q65KY4_BACLD Q65KY4_BACLD]]
-	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.-) catalyze cleavage of alpha-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by beta-elimination. In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions. These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT). The second-generation mutants involved combinations of two to seven individually favorable single mutations. Thermal stability was examined as half-life at 60 degrees C and by recording of thermal transitions by circular dichroism. Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme. A ~ twofold improvement in thermal stability at 60 degrees C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type. The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.	+
-		+
-	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.,Silva IR, Jers C, Otten H, Nyffenegger C, Larsen DM, Derkx PM, Meyer AS, Mikkelsen JD, Larsen S Appl Microbiol Biotechnol. 2014 May;98(10):~~4521-31. doi:, 10.1007~~/~~s00253-013-5483-8. Epub 2014 Jan 14. PMID:24419797<ref>PMID:24419797<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4cag" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium licheniforme weigmann 1898~~]]	+	[[Category: Bacillus licheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jers, C]]	+	[[Category: Jers C]]
-	[[Category: Larsen~~, D M~~]]	+	[[Category: Larsen DM]]
-	[[Category: Larsen, S]]	+	[[Category: Larsen S]]
-	[[Category: Mikkelsen~~, J D~~]]	+	[[Category: Mikkelsen JD]]
-	[[Category: Nyffenegger, C]]	+	[[Category: Nyffenegger C]]
-	[[Category: Otten, H]]	+	[[Category: Otten H]]
-	[[Category: ~~Silva, I I.C~~ Rodrigues da]]	+	[[Category: Rodrigues da Silva IIC]]
-	~~[[Category: Beta-propeller]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 09:10, 20 March 2019

OCA — Wed, 20 Mar 2019 09:10:38 GMT

←Older revision		Revision as of 09:10, 20 March 2019
Line 1:		Line 1:

	==Bacillus licheniformis Rhamnogalacturonan Lyase PL11==		==Bacillus licheniformis Rhamnogalacturonan Lyase PL11==
-	<StructureSection load='4cag' size='340' side='right' caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4cag' size='340' side='right'caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CAG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CAG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://pdbe.org/4cag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://pdbe.org/4cag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium licheniforme weigmann 1898]]
		+	[[Category: Large Structures]]
	[[Category: Jers, C]]		[[Category: Jers, C]]
	[[Category: Larsen, D M]]		[[Category: Larsen, D M]]

OCA at 15:18, 11 August 2016

OCA — Thu, 11 Aug 2016 15:18:30 GMT

←Older revision		Revision as of 15:18, 11 August 2016
Line 1:		Line 1:
		+
	==Bacillus licheniformis Rhamnogalacturonan Lyase PL11==		==Bacillus licheniformis Rhamnogalacturonan Lyase PL11==
	<StructureSection load='4cag' size='340' side='right' caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4cag' size='340' side='right' caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CAG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CAG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://pdbe.org/4cag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cag ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cag" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:15, 25 January 2015

OCA — Sun, 25 Jan 2015 08:15:45 GMT

←Older revision		Revision as of 08:15, 25 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Jers, C.]]	+	[[Category: Jers, C]]
-	[[Category: Larsen, D M.]]	+	[[Category: Larsen, D M]]
-	[[Category: Larsen, S.]]	+	[[Category: Larsen, S]]
-	[[Category: Mikkelsen, J D.]]	+	[[Category: Mikkelsen, J D]]
-	[[Category: Nyffenegger, C.]]	+	[[Category: Nyffenegger, C]]
-	[[Category: Otten, H.]]	+	[[Category: Otten, H]]
-	[[Category: Silva, I I.C Rodrigues da.]]	+	[[Category: Silva, I I.C Rodrigues da]]
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 08:28, 22 October 2014

OCA — Wed, 22 Oct 2014 08:28:51 GMT

←Older revision		Revision as of 08:28, 22 October 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.-) catalyze cleavage of alpha-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by beta-elimination. In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions. These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT). The second-generation mutants involved combinations of two to seven individually favorable single mutations. Thermal stability was examined as half-life at 60 degrees C and by recording of thermal transitions by circular dichroism. Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme. A ~ twofold improvement in thermal stability at 60 degrees C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type. The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
		+
		+	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.,Silva IR, Jers C, Otten H, Nyffenegger C, Larsen DM, Derkx PM, Meyer AS, Mikkelsen JD, Larsen S Appl Microbiol Biotechnol. 2014 May;98(10):4521-31. doi:, 10.1007/s00253-013-5483-8. Epub 2014 Jan 14. PMID:24419797<ref>PMID:24419797</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:50, 20 October 2014

OCA — Mon, 20 Oct 2014 10:50:02 GMT

←Older revision		Revision as of 10:50, 20 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Bacillus licheniformis Rhamnogalacturonan Lyase PL11==
-		+	<StructureSection load='4cag' size='340' side='right' caption='[[4cag]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~The entry~~ 4cag is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cag]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CAG FirstGlance]. <br>
-	~~Authors~~: ~~Otten~~, H., ~~Rodrigues da Silva, I~~.I.C., Jers, C.~~, Nyffenegger, C.,~~ Larsen, D.M., Mikkelsen, J.D., ~~Larsen~~, S.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cag OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cag RCSB], [http://www.ebi.ac.uk/pdbsum/4cag PDBsum]</span></td></tr>
-	~~Description~~: ~~Bacillus licheniformis Rhamnogalacturonan~~ Lyase ~~PL11~~	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jers, C.]]
		+	[[Category: Larsen, D M.]]
		+	[[Category: Larsen, S.]]
		+	[[Category: Mikkelsen, J D.]]
		+	[[Category: Nyffenegger, C.]]
		+	[[Category: Otten, H.]]
		+	[[Category: Silva, I I.C Rodrigues da.]]
		+	[[Category: Beta-propeller]]
		+	[[Category: Lyase]]

OCA at 07:12, 13 November 2013

OCA — Wed, 13 Nov 2013 07:12:46 GMT

←Older revision		Revision as of 07:12, 13 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4cag is ON HOLD	+	The entry 4cag is ON HOLD until Paper Publication

	Authors: Otten, H., Rodrigues da Silva, I.I.C., Jers, C., Nyffenegger, C., Larsen, D.M., Mikkelsen, J.D., Larsen, S.		Authors: Otten, H., Rodrigues da Silva, I.I.C., Jers, C., Nyffenegger, C., Larsen, D.M., Mikkelsen, J.D., Larsen, S.

	Description: Bacillus licheniformis Rhamnogalacturonan Lyase PL11		Description: Bacillus licheniformis Rhamnogalacturonan Lyase PL11

OCA: Protected "4cag" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2013 07:09:21 GMT

Protected "4cag" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:09, 16 October 2013

OCA: New page: '''Unreleased structure''' The entry 4cag is ON HOLD Authors: Otten, H., Rodrigues da Silva, I.I.C., Jers, C., Nyffenegger, C., Larsen, D.M., Mikkelsen, J.D., Larsen, S. Description: B...

OCA — Wed, 16 Oct 2013 07:09:20 GMT

New page: '''Unreleased structure''' The entry 4cag is ON HOLD Authors: Otten, H., Rodrigues da Silva, I.I.C., Jers, C., Nyffenegger, C., Larsen, D.M., Mikkelsen, J.D., Larsen, S. Description: B...

New page

'''Unreleased structure'''

The entry 4cag is ON HOLD

Authors: Otten, H., Rodrigues da Silva, I.I.C., Jers, C., Nyffenegger, C., Larsen, D.M., Mikkelsen, J.D., Larsen, S.

Description: Bacillus licheniformis Rhamnogalacturonan Lyase PL11