4cdb - Revision history

OCA at 12:08, 20 December 2023

OCA — Wed, 20 Dec 2023 12:08:11 GMT

←Older revision		Revision as of 12:08, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [https://pdbe.org/4cdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [https://www.ebi.ac.uk/pdbsum/4cdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [https://pdbe.org/4cdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [https://www.ebi.ac.uk/pdbsum/4cdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A0H3GD84_LISM4 A0A0H3GD84_LISM4]] A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.[RuleBase:RU364025]	+	[https://www.uniprot.org/uniprot/A0A0H3GD84_LISM4 A0A0H3GD84_LISM4] A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.[RuleBase:RU364025]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Listeriolysin O (LLO) is an essential virulence factor of Listeria monocytogenes that causes listeriosis. Listeria monocytogenes owes its ability to live within cells to the pH- and temperature-dependent pore-forming activity of LLO, which is unique among cholesterol-dependent cytolysins. LLO enables the bacteria to cross the phagosomal membrane and is also involved in activation of cellular processes, including the modulation of gene expression or intracellular Ca(2+) oscillations. Neither the pore-forming mechanism nor the mechanisms triggering the signalling processes in the host cell are known in detail. Here, we report the crystal structure of LLO, in which we identified regions important for oligomerization and pore formation. Mutants were characterized by determining their haemolytic and Ca(2+) uptake activity. We analysed the pore formation of LLO and its variants on erythrocyte ghosts by electron microscopy and show that pore formation requires precise interface interactions during toxin oligomerization on the membrane.
		+
		+	Crystal structure of listeriolysin O reveals molecular details of oligomerization and pore formation.,Koster S, van Pee K, Hudel M, Leustik M, Rhinow D, Kuhlbrandt W, Chakraborty T, Yildiz O Nat Commun. 2014 Apr 22;5:3690. doi: 10.1038/ncomms4690. PMID:24751541<ref>PMID:24751541</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cdb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cytolysin 3D structures\|Cytolysin 3D structures]]		*[[Cytolysin 3D structures\|Cytolysin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:40, 7 September 2022

OCA — Wed, 07 Sep 2022 17:40:06 GMT

←Older revision		Revision as of 17:40, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4cdb' size='340' side='right'caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4cdb' size='340' side='right'caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CDB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [~~http~~://pdbe.org/4cdb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cdb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [https://pdbe.org/4cdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [https://www.ebi.ac.uk/pdbsum/4cdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdb ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/A0A0H3GD84_LISM4 A0A0H3GD84_LISM4]] A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.[RuleBase:RU364025]
-	~~Listeriolysin O (LLO) is an essential virulence factor of Listeria monocytogenes that causes listeriosis~~. ~~Listeria monocytogenes owes its ability to live within cells to the pH- and temperature~~-dependent ~~pore-~~forming ~~activity of LLO, which is unique among~~ cholesterol~~-dependent cytolysins~~. ~~LLO enables the bacteria~~ to ~~cross~~ the ~~phagosomal membrane and is also involved in activation of cellular processes~~, ~~including the modulation of gene expression or intracellular Ca(2+) oscillations. Neither the pore-forming mechanism nor the mechanisms triggering the signalling processes~~ in the host ~~cell are known in detail. Here, we report the crystal structure of LLO, in which we identified regions important for oligomerization~~ and ~~pore formation. Mutants were characterized by determining their haemolytic and Ca(2+) uptake activity. We analysed the pore~~ formation of ~~LLO and its variants on erythrocyte ghosts by electron microscopy and show that~~ pore ~~formation requires precise interface interactions during toxin oligomerization on the~~ membrane.	+
-		+
-	~~Crystal structure of listeriolysin O reveals molecular details of oligomerization~~ and pore formation~~.,Koster S, van Pee K, Hudel M, Leustik M, Rhinow D, Kuhlbrandt W, Chakraborty T, Yildiz O Nat Commun. 2014 Apr 22~~;~~5:3690. doi: 10.1038/ncomms4690. PMID:24751541<ref>PMID:24751541</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®,~~ a ~~database of~~ the U.S. ~~National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4cdb" style="background-color~~:~~#fffaf0;"></div>~~	+

	==See Also==		==See Also==
-	*[[Cytolysin\|Cytolysin]]	+	*[[Cytolysin 3D structures\|Cytolysin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Listeria monocytogenes]]		[[Category: Listeria monocytogenes]]
-	[[Category: Koester, S]]	+	[[Category: Koester S]]
-	[[Category: Yildiz, O]]	+	[[Category: Yildiz O]]
-	~~[[Category: Cholesterol dependent cytolysin]]~~	+
-	~~[[Category: Hemolysis]]~~	+
-	~~[[Category: Membrane perforation]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:01, 20 March 2019

OCA — Wed, 20 Mar 2019 10:01:48 GMT

←Older revision		Revision as of 10:01, 20 March 2019
Line 1:		Line 1:

	==Crystal structure of listeriolysin O==		==Crystal structure of listeriolysin O==
-	<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4cdb' size='340' side='right'caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDB FirstGlance]. <br>
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 4cdb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cdb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytolysin\|Cytolysin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Listeria monocytogenes]]		[[Category: Listeria monocytogenes]]
	[[Category: Koester, S]]		[[Category: Koester, S]]

OCA at 20:28, 11 August 2016

OCA — Thu, 11 Aug 2016 20:28:30 GMT

←Older revision		Revision as of 20:28, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of listeriolysin O==		==Crystal structure of listeriolysin O==
	<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://pdbe.org/4cdb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cdb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:31, 4 January 2015

OCA — Sun, 04 Jan 2015 13:31:24 GMT

←Older revision		Revision as of 13:31, 4 January 2015
Line 2:		Line 2:
	<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>	+	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene~~><br>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 12:
	Crystal structure of listeriolysin O reveals molecular details of oligomerization and pore formation.,Koster S, van Pee K, Hudel M, Leustik M, Rhinow D, Kuhlbrandt W, Chakraborty T, Yildiz O Nat Commun. 2014 Apr 22;5:3690. doi: 10.1038/ncomms4690. PMID:24751541<ref>PMID:24751541</ref>		Crystal structure of listeriolysin O reveals molecular details of oligomerization and pore formation.,Koster S, van Pee K, Hudel M, Leustik M, Rhinow D, Kuhlbrandt W, Chakraborty T, Yildiz O Nat Commun. 2014 Apr 22;5:3690. doi: 10.1038/ncomms4690. PMID:24751541<ref>PMID:24751541</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Listeria monocytogenes]]		[[Category: Listeria monocytogenes]]
-	[[Category: Koester, S.]]	+	[[Category: Koester, S]]
-	[[Category: Yildiz, O.]]	+	[[Category: Yildiz, O]]
	[[Category: Cholesterol dependent cytolysin]]		[[Category: Cholesterol dependent cytolysin]]
	[[Category: Hemolysis]]		[[Category: Hemolysis]]
	[[Category: Membrane perforation]]		[[Category: Membrane perforation]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 07:09, 7 May 2014

OCA — Wed, 07 May 2014 07:09:09 GMT

←Older revision		Revision as of 07:09, 7 May 2014
Line 7:		Line 7:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Listeriolysin O (LLO) is an essential virulence factor of Listeria monocytogenes that causes listeriosis. Listeria monocytogenes owes its ability to live within cells to the pH- and temperature-dependent pore-forming activity of LLO, which is unique among cholesterol-dependent cytolysins. LLO enables the bacteria to cross the phagosomal membrane and is also involved in activation of cellular processes, including the modulation of gene expression or intracellular Ca(2+) oscillations. Neither the pore-forming mechanism nor the mechanisms triggering the signalling processes in the host cell are known in detail. Here, we report the crystal structure of LLO, in which we identified regions important for oligomerization and pore formation. Mutants were characterized by determining their haemolytic and Ca(2+) uptake activity. We analysed the pore formation of LLO and its variants on erythrocyte ghosts by electron microscopy and show that pore formation requires precise interface interactions during toxin oligomerization on the membrane.
		+
		+	Crystal structure of listeriolysin O reveals molecular details of oligomerization and pore formation.,Koster S, van Pee K, Hudel M, Leustik M, Rhinow D, Kuhlbrandt W, Chakraborty T, Yildiz O Nat Commun. 2014 Apr 22;5:3690. doi: 10.1038/ncomms4690. PMID:24751541<ref>PMID:24751541</ref>
		+
		+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:38, 1 May 2014

OCA — Thu, 01 May 2014 09:38:13 GMT

←Older revision		Revision as of 09:38, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>	+	<table><tr><td colspan='2'>[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>
-	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene><br>	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene><br>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span></td></tr>
		+	<table>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:12, 30 April 2014

OCA — Wed, 30 Apr 2014 10:12:17 GMT

←Older revision		Revision as of 10:12, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>		[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>
		+	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene><br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdb RCSB], [http://www.ebi.ac.uk/pdbsum/4cdb PDBsum]</span><br>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:34, 30 April 2014

OCA — Wed, 30 Apr 2014 05:34:07 GMT

←Older revision		Revision as of 05:34, 30 April 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4cdb\| PDB=4cdb \| SCENE= }}~~	+	==Crystal structure of listeriolysin O==
-	===Crystal structure of listeriolysin O===	+	<StructureSection load='4cdb' size='340' side='right' caption='[[4cdb]], [[Resolution\|resolution]] 2.15Å' scene=''>
-		+	== Structural highlights ==
-	==~~About this Structure~~==	+	[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA]. <br>
-	[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA].	+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Listeria monocytogenes]]		[[Category: Listeria monocytogenes]]
	[[Category: Koester, S.]]		[[Category: Koester, S.]]

OCA at 07:51, 23 April 2014

OCA — Wed, 23 Apr 2014 07:51:06 GMT

←Older revision		Revision as of 07:51, 23 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4cdb\| PDB=4cdb \| SCENE= }}
		+	===Crystal structure of listeriolysin O===

-	~~The entry~~ 4cdb is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4cdb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDB OCA].
-	~~Authors~~: Koester, S., Yildiz, O.	+	[[Category: Listeria monocytogenes]]
-		+	[[Category: Koester, S.]]
-	~~Description~~: ~~Crystal structure of listeriolysin O~~	+	[[Category: Yildiz, O.]]
		+	[[Category: Cholesterol dependent cytolysin]]
		+	[[Category: Hemolysis]]
		+	[[Category: Membrane perforation]]
		+	[[Category: Toxin]]