4cdg - Revision history

OCA at 12:08, 20 December 2023

OCA — Wed, 20 Dec 2023 12:08:19 GMT

←Older revision		Revision as of 12:08, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.794Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [https://pdbe.org/4cdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [https://www.ebi.ac.uk/pdbsum/4cdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [https://pdbe.org/4cdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [https://www.ebi.ac.uk/pdbsum/4cdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdg ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>	+	[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.
		+
		+	Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.,Newman JA, Savitsky P, Allerston CK, Bizard AH, Ozer O, Sarlos K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O Nucleic Acids Res. 2015 Apr 21. pii: gkv373. PMID:25901030<ref>PMID:25901030</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 17:40, 7 September 2022

OCA — Wed, 07 Sep 2022 17:40:34 GMT

←Older revision		Revision as of 17:40, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4cdg' size='340' side='right'caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='4cdg' size='340' side='right'caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus glama~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CDG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CDG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [https://pdbe.org/4cdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [https://www.ebi.ac.uk/pdbsum/4cdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [~~http~~://pdbe.org/4cdg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cdg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.	+	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>	+	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.	+
-		+
-	Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.,Newman JA, Savitsky P, Allerston CK, Bizard AH, Ozer O, Sarlos K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O Nucleic Acids Res. 2015 Apr 21. pii: gkv373. PMID:25901030<ref>PMID:25901030</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus glama~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~DNA helicase]]~~	+	[[Category: Lama glama]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allerston~~, C K~~]]	+	[[Category: Allerston CK]]
-	[[Category: Arrowsmith~~, C H~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: Bountra, C]]	+	[[Category: Bountra C]]
-	~~[[Category: Delft, F von~~]]	+	[[Category: Edwards A]]
-	[[Category: Edwards, A]]	+	[[Category: Gileadi O]]
-	[[Category: Gileadi, O]]	+	[[Category: Newman JA]]
-	[[Category: Newman~~, J A~~]]	+	[[Category: Pardon E]]
-	[[Category: Pardon, E]]	+	[[Category: Pike ACW]]
-	[[Category: Pike~~, A C.W~~]]	+	[[Category: Savitsky P]]
-	[[Category: Savitsky, P]]	+	[[Category: Steyaert J]]
-	[[Category: Steyaert, J]]	+	[[Category: Von Delft F]]
-	[[Category: ~~Hydrolase~~]]	+

OCA at 09:32, 5 February 2020

OCA — Wed, 05 Feb 2020 09:32:29 GMT

←Older revision		Revision as of 09:32, 5 February 2020
Line 1:		Line 1:

	==Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody==		==Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody==
-	<StructureSection load='4cdg' size='340' side='right' caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>	+	<StructureSection load='4cdg' size='340' side='right'caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDG FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 31:
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Allerston, C K]]		[[Category: Allerston, C K]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]

OCA at 19:48, 24 January 2018

OCA — Wed, 24 Jan 2018 19:48:12 GMT

←Older revision		Revision as of 19:48, 24 January 2018
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Helicase\|Helicase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 31:
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
	[[Category: Bountra, C]]		[[Category: Bountra, C]]
-	[[Category: Delft, F ~~Von~~]]	+	[[Category: Delft, F von]]
	[[Category: Edwards, A]]		[[Category: Edwards, A]]
	[[Category: Gileadi, O]]		[[Category: Gileadi, O]]

OCA at 02:03, 11 March 2017

OCA — Sat, 11 Mar 2017 02:03:38 GMT

←Older revision		Revision as of 02:03, 11 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody==		==Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody==
	<StructureSection load='4cdg' size='340' side='right' caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='4cdg' size='340' side='right' caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [http://www.ebi.ac.uk/pdbsum/4cdg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [http://pdbe.org/4cdg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [http://www.ebi.ac.uk/pdbsum/4cdg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cdg ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cdg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:20, 6 May 2015

OCA — Wed, 06 May 2015 07:20:01 GMT

←Older revision		Revision as of 07:20, 6 May 2015
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>		[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.
		+
		+	Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.,Newman JA, Savitsky P, Allerston CK, Bizard AH, Ozer O, Sarlos K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O Nucleic Acids Res. 2015 Apr 21. pii: gkv373. PMID:25901030<ref>PMID:25901030</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+
		+	==See Also==
		+	*[[Helicase\|Helicase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:44, 21 December 2014

OCA — Sun, 21 Dec 2014 16:44:03 GMT

←Older revision		Revision as of 16:44, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4cdg\| PDB=4cdg \| SCENE= }}~~	+	==Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody==
-	===Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody===	+	<StructureSection load='4cdg' size='340' side='right' caption='[[4cdg]], [[Resolution\|resolution]] 2.79Å' scene=''>
-		+	== Structural highlights ==
-	==Disease==	+	<table><tr><td colspan='2'>[[4cdg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CDG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cdg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cdg RCSB], [http://www.ebi.ac.uk/pdbsum/4cdg PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.		[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
-		+	== Function ==
-	==Function==	+
	[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>		[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
-		+	== References ==
-	==~~About this Structure==~~	+	<references/>
-	[[4cdg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA].	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Camelus glama]]		[[Category: Camelus glama]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Allerston, C K.]]	+	[[Category: Allerston, C K]]
-	[[Category: Arrowsmith, C H.]]	+	[[Category: Arrowsmith, C H]]
-	[[Category: Bountra, C.]]	+	[[Category: Bountra, C]]
-	[[Category: Delft, F Von.]]	+	[[Category: Delft, F Von]]
-	[[Category: Edwards, A.]]	+	[[Category: Edwards, A]]
-	[[Category: Gileadi, O.]]	+	[[Category: Gileadi, O]]
-	[[Category: Newman, J A.]]	+	[[Category: Newman, J A]]
-	[[Category: Pardon, E.]]	+	[[Category: Pardon, E]]
-	[[Category: Pike, A C.W.]]	+	[[Category: Pike, A C.W]]
-	[[Category: Savitsky, P.]]	+	[[Category: Savitsky, P]]
-	[[Category: Steyaert, J.]]	+	[[Category: Steyaert, J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:25, 8 January 2014

OCA — Wed, 08 Jan 2014 08:25:25 GMT

←Older revision		Revision as of 08:25, 8 January 2014
Line 9:		Line 9:

	==About this Structure==		==About this Structure==
-	[[4cdg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA].	+	[[4cdg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA].

	==Reference==		==Reference==
	<references group="xtra"/><references/>		<references group="xtra"/><references/>
		+	[[Category: Camelus glama]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
		+	[[Category: Human]]
	[[Category: Allerston, C K.]]		[[Category: Allerston, C K.]]
	[[Category: Arrowsmith, C H.]]		[[Category: Arrowsmith, C H.]]

OCA: Protected "4cdg" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2013 08:26:39 GMT

Protected "4cdg" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:26, 13 November 2013

OCA: New page: {{STRUCTURE_4cdg| PDB=4cdg | SCENE= }} ===Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody=== ==Disease== [[http://www.uniprot.org/uniprot/BLM_HUMAN BL...

OCA — Wed, 13 Nov 2013 08:26:37 GMT

New page: {{STRUCTURE_4cdg| PDB=4cdg | SCENE= }} ===Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody=== ==Disease== [[http://www.uniprot.org/uniprot/BLM_HUMAN BL...

New page

{{STRUCTURE_4cdg| PDB=4cdg | SCENE= }}
===Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody===

==Disease==
[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.

==Function==
[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>

==About this Structure==
[[4cdg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CDG OCA].

==Reference==
<references group="xtra"/><references/>
[[Category: DNA helicase]]
[[Category: Allerston, C K.]]
[[Category: Arrowsmith, C H.]]
[[Category: Bountra, C.]]
[[Category: Delft, F Von.]]
[[Category: Edwards, A.]]
[[Category: Gileadi, O.]]
[[Category: Newman, J A.]]
[[Category: Pardon, E.]]
[[Category: Pike, A C.W.]]
[[Category: Savitsky, P.]]
[[Category: Steyaert, J.]]
[[Category: Hydrolase]]