4cfi - Revision history

OCA at 12:09, 20 December 2023

OCA — Wed, 20 Dec 2023 12:09:22 GMT

←Older revision		Revision as of 12:09, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CFI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [https://pdbe.org/4cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [https://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [https://pdbe.org/4cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [https://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/P70944_BURPE P70944_BURPE]]	+	[https://www.uniprot.org/uniprot/P70944_BURPE P70944_BURPE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.
		+
		+	Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.,Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio-Sole O, Ferrer-Navarro M, Gourlay LJ, Nardini M, Vila J, Daura X, Colombo G, Bolognesi M, Lertmemonkolchai G PLoS Negl Trop Dis. 2015 Jul 29;9(7):e0003917. doi: 10.1371/journal.pntd.0003917., eCollection 2015 Jul. PMID:26222657<ref>PMID:26222657</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Flagellin 3D structures\|Flagellin 3D structures]]		*[[Flagellin 3D structures\|Flagellin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:43, 7 September 2022

OCA — Wed, 07 Sep 2022 17:43:06 GMT

←Older revision		Revision as of 17:43, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4cfi' size='340' side='right'caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4cfi' size='340' side='right'caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burps Burps~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [~~http~~://pdbe.org/4cfi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [https://pdbe.org/4cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [https://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/P70944_BURPE P70944_BURPE]]
-	Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.	+
-		+
-	Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.,Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio-Sole O, Ferrer-Navarro M, Gourlay LJ, Nardini M, Vila J, Daura X, Colombo G, Bolognesi M, Lertmemonkolchai G PLoS Negl Trop Dis. 2015 Jul 29;9(7):~~e0003917. doi: 10.1371~~/~~journal.pntd.0003917., eCollection 2015 Jul. PMID:26222657<ref>PMID:26222657<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
-	*[[Flagellin\|Flagellin]]	+	*[[Flagellin 3D structures\|Flagellin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Burps~~]]	+	[[Category: Burkholderia pseudomallei K96243]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Colombo, G]]	+	[[Category: Colombo G]]
-	[[Category: Conchillo-Sole, O]]	+	[[Category: Conchillo-Sole O]]
-	[[Category: Daura, X]]	+	[[Category: Daura X]]
-	[[Category: Ferrer-Navarro, M]]	+	[[Category: Ferrer-Navarro M]]
-	[[Category: Gourlay~~, L J~~]]	+	[[Category: Gourlay LJ]]
-	[[Category: Lassaux, P]]	+	[[Category: Lassaux P]]
-	[[Category: Peri, C]]	+	[[Category: Peri C]]
-	~~[[Category: Epitope]]~~	+
-	~~[[Category: Flagella]]~~	+
-	~~[[Category: Immune response]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 10:02, 20 March 2019

OCA — Wed, 20 Mar 2019 10:02:03 GMT

←Older revision		Revision as of 10:02, 20 March 2019
Line 1:		Line 1:

	==3D structure of FliC from Burkholderia pseudomallei==		==3D structure of FliC from Burkholderia pseudomallei==
-	<StructureSection load='4cfi' size='340' side='right' caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='4cfi' size='340' side='right'caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [http://pdbe.org/4cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [http://www.ebi.ac.uk/pdbsum/4cfi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [http://pdbe.org/4cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [http://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellin\|Flagellin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burps]]
		+	[[Category: Large Structures]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Colombo, G]]		[[Category: Colombo, G]]

OCA at 07:08, 2 March 2016

OCA — Wed, 02 Mar 2016 07:08:38 GMT

←Older revision		Revision as of 07:08, 2 March 2016
Line 1:		Line 1:
		+
	==3D structure of FliC from Burkholderia pseudomallei==		==3D structure of FliC from Burkholderia pseudomallei==
	<StructureSection load='4cfi' size='340' side='right' caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4cfi' size='340' side='right' caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CFI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [http://www.ebi.ac.uk/pdbsum/4cfi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [http://pdbe.org/4cfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [http://www.ebi.ac.uk/pdbsum/4cfi PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.
		+
		+	Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.,Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio-Sole O, Ferrer-Navarro M, Gourlay LJ, Nardini M, Vila J, Daura X, Colombo G, Bolognesi M, Lertmemonkolchai G PLoS Negl Trop Dis. 2015 Jul 29;9(7):e0003917. doi: 10.1371/journal.pntd.0003917., eCollection 2015 Jul. PMID:26222657<ref>PMID:26222657</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:17, 10 December 2014

OCA — Wed, 10 Dec 2014 16:17:32 GMT

←Older revision		Revision as of 16:17, 10 December 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==3D structure of FliC from Burkholderia pseudomallei==
-		+	<StructureSection load='4cfi' size='340' side='right' caption='[[4cfi]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~The entry~~ 4cfi is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CFI FirstGlance]. <br>
-	~~Authors~~: ~~Lassaux, P~~.~~, Peri, C~~., ~~Ferrer~~-~~Navarro~~, M., ~~Gourlay, L~~.J., Conchillo-Sole, O., Daura, X., ~~Colombo~~, G., ~~Bolognesi~~, M.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [http://www.ebi.ac.uk/pdbsum/4cfi PDBsum]</span></td></tr>
-		+	</table>
-	~~Description~~: ~~3D structure of FliC from Burkholderia pseudomallei~~	+	__TOC__
		+	</StructureSection>
		+	[[Category: Bolognesi, M]]
		+	[[Category: Colombo, G]]
		+	[[Category: Conchillo-Sole, O]]
		+	[[Category: Daura, X]]
		+	[[Category: Ferrer-Navarro, M]]
		+	[[Category: Gourlay, L J]]
		+	[[Category: Lassaux, P]]
		+	[[Category: Peri, C]]
		+	[[Category: Epitope]]
		+	[[Category: Flagella]]
		+	[[Category: Immune response]]
		+	[[Category: Structural protein]]

OCA: Protected "4cfi" [edit=sysop:move=sysop]

OCA — Wed, 27 Nov 2013 09:19:48 GMT

Protected "4cfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 27 November 2013

OCA: New page: '''Unreleased structure''' The entry 4cfi is ON HOLD until Paper Publication Authors: Lassaux, P., Peri, C., Ferrer-Navarro, M., Gourlay, L.J., Conchillo-Sole, O., Daura, X., Colombo, G...

OCA — Wed, 27 Nov 2013 09:19:46 GMT

New page: '''Unreleased structure''' The entry 4cfi is ON HOLD until Paper Publication Authors: Lassaux, P., Peri, C., Ferrer-Navarro, M., Gourlay, L.J., Conchillo-Sole, O., Daura, X., Colombo, G...

New page

'''Unreleased structure'''

The entry 4cfi is ON HOLD until Paper Publication

Authors: Lassaux, P., Peri, C., Ferrer-Navarro, M., Gourlay, L.J., Conchillo-Sole, O., Daura, X., Colombo, G., Bolognesi, M.

Description: 3D structure of FliC from Burkholderia pseudomallei