4cpe - Revision history

OCA at 12:14, 20 December 2023

OCA — Wed, 20 Dec 2023 12:14:20 GMT

←Older revision		Revision as of 12:14, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CPE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CPE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.06Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [https://pdbe.org/4cpe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [https://www.ebi.ac.uk/pdbsum/4cpe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cpe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [https://pdbe.org/4cpe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [https://www.ebi.ac.uk/pdbsum/4cpe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cpe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:26, 14 September 2022

OCA — Wed, 14 Sep 2022 07:26:07 GMT

←Older revision		Revision as of 07:26, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4cpe' size='340' side='right'caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>		<StructureSection load='4cpe' size='340' side='right'caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CPE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CPE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [https://pdbe.org/4cpe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [https://www.ebi.ac.uk/pdbsum/4cpe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cpe ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [~~http~~://pdbe.org/4cpe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cpe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cpe ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chan~~, L K.M~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Donohoe~~, T J~~]]	+	[[Category: Chan LKM]]
-	[[Category: Fairhead, M]]	+	[[Category: Donohoe TJ]]
-	[[Category: Howarth, M]]	+	[[Category: Fairhead M]]
-	[[Category: Lowe~~, E D~~]]	+	[[Category: Howarth M]]
-	[[Category: Shen, D]]	+	[[Category: Lowe ED]]
-	~~[[Category: Affinity]]~~	+	[[Category: Shen D]]
-	~~[[Category: Avidin]]~~	+
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Biotin-binding protein]]~~	+
-	~~[[Category: Biotinylated]]~~	+
-	~~[[Category: Force]]~~	+
-	~~[[Category: Hindered]]~~	+
-	~~[[Category: Ligand series]]~~	+
-	~~[[Category: Steric clash]]~~	+
-	~~[[Category: Strain]]~~	+
-	~~[[Category: Strained~~]]	+

OCA at 12:50, 10 May 2019

OCA — Fri, 10 May 2019 12:50:15 GMT

←Older revision		Revision as of 12:50, 10 May 2019
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4cpe" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cpe" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:49, 20 March 2019

OCA — Wed, 20 Mar 2019 08:49:26 GMT

←Older revision		Revision as of 08:49, 20 March 2019
Line 1:		Line 1:

	==Wild-type streptavidin in complex with love-hate ligand 1 (LH1)==		==Wild-type streptavidin in complex with love-hate ligand 1 (LH1)==
-	<StructureSection load='4cpe' size='340' side='right' caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>	+	<StructureSection load='4cpe' size='340' side='right'caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CPE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CPE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Chan, L K.M]]		[[Category: Chan, L K.M]]
	[[Category: Donohoe, T J]]		[[Category: Donohoe, T J]]

OCA at 13:03, 5 August 2016

OCA — Fri, 05 Aug 2016 13:03:07 GMT

←Older revision		Revision as of 13:03, 5 August 2016
Line 1:		Line 1:
		+
	==Wild-type streptavidin in complex with love-hate ligand 1 (LH1)==		==Wild-type streptavidin in complex with love-hate ligand 1 (LH1)==
	<StructureSection load='4cpe' size='340' side='right' caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>		<StructureSection load='4cpe' size='340' side='right' caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://pdbe.org/4cpe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cpe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cpe" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:41, 25 December 2014

OCA — Thu, 25 Dec 2014 02:41:47 GMT

←Older revision		Revision as of 02:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CPE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CPE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chan, L K.M.]]	+	[[Category: Chan, L K.M]]
-	[[Category: Donohoe, T J.]]	+	[[Category: Donohoe, T J]]
-	[[Category: Fairhead, M.]]	+	[[Category: Fairhead, M]]
-	[[Category: Howarth, M.]]	+	[[Category: Howarth, M]]
-	[[Category: Lowe, E D.]]	+	[[Category: Lowe, E D]]
-	[[Category: Shen, D.]]	+	[[Category: Shen, D]]
	[[Category: Affinity]]		[[Category: Affinity]]
	[[Category: Avidin]]		[[Category: Avidin]]

OCA at 05:56, 27 August 2014

OCA — Wed, 27 Aug 2014 05:56:05 GMT

←Older revision		Revision as of 05:56, 27 August 2014
Line 7:		Line 7:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The pathway of ligand dissociation and how binding sites respond to force are not well understood for any macromolecule. Force effects on biological receptors have been studied through simulation or force spectroscopy, but not by high resolution structural experiments. To investigate this challenge, we took advantage of the extreme stability of the streptavidin-biotin interaction, a paradigm for understanding non-covalent binding as well as a ubiquitous research tool. We synthesized a series of biotin-conjugates having an unchanged strong-binding biotin moiety, along with pincer-like arms designed to clash with the protein surface: 'Love-Hate ligands'. The Love-Hate ligands contained various 2,6-di-ortho aryl groups, installed using Suzuki coupling as the last synthetic step, making the steric repulsion highly modular. We determined binding affinity, as well as solving 1.1-1.6A resolution crystal structures of streptavidin bound to Love-Hate ligands. Striking distortion of streptavidin's binding contacts was found for these complexes. Hydrogen bonds to biotin's ureido and thiophene rings were preserved for all the ligands, but biotin's valeryl tail was distorted from the classic conformation. Streptavidin's L3/4 loop, normally forming multiple energetically-important hydrogen bonds to biotin, was forced away by clashes with Love-Hate ligands, but Ser45 from L3/4 could adapt to hydrogen-bond to a different part of the ligand. This approach of preparing conflicted ligands represents a direct way to visualize strained biological interactions and test protein plasticity.
		+
		+	Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction.,Fairhead M, Shen D, Chan LK, Lowe ED, Donohoe TJ, Howarth M Bioorg Med Chem. 2014 Jul 24. pii: S0968-0896(14)00545-8. doi:, 10.1016/j.bmc.2014.07.029. PMID:25128469<ref>PMID:25128469</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 02:37, 7 August 2014

OCA — Thu, 07 Aug 2014 02:37:19 GMT

←Older revision		Revision as of 02:37, 7 August 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Wild-type streptavidin in complex with love-hate ligand 1 (LH1)==
-		+	<StructureSection load='4cpe' size='340' side='right' caption='[[4cpe]], [[Resolution\|resolution]] 1.06Å' scene=''>
-	~~The entry~~ 4cpe is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cpe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CPE FirstGlance]. <br>
-	~~Authors~~: ~~Fairhead~~, M., ~~Shen~~, D., ~~Chan~~, L.K.M., ~~Lowe~~, E.D., Donohoe, T.J., Howarth, M.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LUV:(3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-+D]IMIDAZOLIDIN-4-YL]-N-{2-[(2,6-+DIPHENYLPHENYL)FORMAMIDO]ETHYL}PENTANAMIDE'>LUV</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cpf\|4cpf]], [[4cph\|4cph]], [[4cpi\|4cpi]]</td></tr>
-	~~Description~~: ~~Wild~~-~~type streptavidin in complex with love-hate ligand 1 (LH1)~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cpe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cpe RCSB], [http://www.ebi.ac.uk/pdbsum/4cpe PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chan, L K.M.]]
		+	[[Category: Donohoe, T J.]]
		+	[[Category: Fairhead, M.]]
		+	[[Category: Howarth, M.]]
		+	[[Category: Lowe, E D.]]
		+	[[Category: Shen, D.]]
		+	[[Category: Affinity]]
		+	[[Category: Avidin]]
		+	[[Category: Biotin]]
		+	[[Category: Biotin-binding protein]]
		+	[[Category: Biotinylated]]
		+	[[Category: Force]]
		+	[[Category: Hindered]]
		+	[[Category: Ligand series]]
		+	[[Category: Steric clash]]
		+	[[Category: Strain]]
		+	[[Category: Strained]]

OCA at 06:28, 9 July 2014

OCA — Wed, 09 Jul 2014 06:28:30 GMT

←Older revision		Revision as of 06:28, 9 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4cpe is ON HOLD	+	The entry 4cpe is ON HOLD until Paper Publication

	Authors: Fairhead, M., Shen, D., Chan, L.K.M., Lowe, E.D., Donohoe, T.J., Howarth, M.		Authors: Fairhead, M., Shen, D., Chan, L.K.M., Lowe, E.D., Donohoe, T.J., Howarth, M.

	Description: Wild-type streptavidin in complex with love-hate ligand 1 (LH1)		Description: Wild-type streptavidin in complex with love-hate ligand 1 (LH1)

OCA: Protected "4cpe" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2014 04:44:55 GMT

Protected "4cpe" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:44, 13 February 2014